Diseño y manufactura de pie Protésico

El término prótesis hace referencia al dispositivo que sustituye o compensa la pérdida de un miembro perdido, para el caso objeto de estudio, se da en una prótesis de miembro inferior, en el caso de una amputación de miembros inferiores a nivel transtibial, el pie es el elemento ausente más significativo ya que asume la estabilidad mínima del cuerpo del paciente, además de interferir en el balance intrínseco del cuerpo humano. Por lo anteriormente descrito uno de los pilares más importantes de la investigación consiste en el desarrollo y ensamble de un pie protésico que cumpla con las características morfológicas, físicas y mecánicas de pie a sustituir en las prótesis propias de nuestros pacientes caso de estudio

Hematological parameters in Oreochromis niloticus cultivated in tropical conditions in experimental farm Santo Domingo of University of Amazonia, Florencia, Caquetá, Colombia.

The hematological parameters were evaluated in the Oreochromis niloticus species, weighing between 120 and 375 g, where chemical standards of water were included (T °, pH and OD), morphometry and haematological diagnosis, carried out at the Santo Domingo Experimental Farm from the University of the Amazonia, Florencia - Caquetá-Colombia. A directed and completely random sampling of 32 individuals was used, the blood was extracted by cardiac puncture and placed in Eppendorf tubes with EDTA. Blood smears were stained with Ema staining color for its laboratory analysis. The InfoStat was applied as a statistical method for the analysis of the variables to be measured. A total of leukocytes of 1.11 celx105 / mm3 was found, predominating neutrophils with 49.3% followed by lymphocytes with 38.8%, monocytes with 5.9%, eosinophils with 4.3%, basophils with 1.7 % and thrombocytes with 63.8% as an independent series. For the red series, an average of erythrocytes of 1.5 celx106 / mm3, hemoglobin of 7.9 g / dL; hematocrit of 23.3%, plasma proteins 3.3 g / dL, mean corpuscular volume 165 (u3), mean corpuscular hemoglobin 55 (uug) and mean corpuscular hemoglobin concentration of 33.3%. In conclusion, it was determined that some individuals presented Leukopenia with Lymphopenia and thrombocytosis. In addition, two individuals presented Normochromic Microcytic Anemia (AMN) and 19 individuals presented Normochromic Normocitic Anemia (ANN). The analysis of Total Plasma Proteins (PPT) determined that the high mortality rate was not due to nutritional deficiency. There was a positive similarity between the VCM and HCM, and negatively with the total number of erythrocytes.The hematological parameters were evaluated in the Oreochromis niloticus species, weighing between 120 and 375 g, where chemical standards of water were included (T °, pH and OD), morphometry and haematological diagnosis, carried out at the Santo Domingo Experimental Farm from the University of the Amazonia, Florencia - Caquetá-Colombia. A directed and completely random sampling of 32 individuals was used, the blood was extracted by cardiac puncture and placed in Eppendorf tubes with EDTA. Blood smears were stained with Ema staining color for its laboratory analysis. The InfoStat was applied as a statistical method for the analysis of the variables to be measured. A total of leukocytes of 1.11 celx105 / mm3 was found, predominating neutrophils with 49.3% followed by lymphocytes with 38.8%, monocytes with 5.9%, eosinophils with 4.3%, basophils with 1.7 % and thrombocytes with 63.8% as an independent series. For the red series, an average of erythrocytes of 1.5 celx106 / mm3, hemoglobin of 7.9 g / dL; hematocrit of 23.3%, plasma proteins 3.3 g / dL, mean corpuscular volume 165 (u3), mean corpuscular hemoglobin 55 (uug) and mean corpuscular hemoglobin concentration of 33.3%. In conclusion, it was determined that some individuals presented Leukopenia with Lymphopenia and thrombocytosis. In addition, two individuals presented Normochromic Microcytic Anemia (AMN) and 19 individuals presented Normochromic Normocitic Anemia (ANN). The analysis of Total Plasma Proteins (PPT) determined that the high mortality rate was not due to nutritional deficiency. There was a positive similarity between the VCM and HCM, and negatively with the total number of erythrocyte

Hematological parameters in Oreochromis niloticus cultivated in tropical conditions in experimental farm Santo Domingo of University of Amazonia, Florencia, Caquetá, Colombia.

The hematological parameters were evaluated in the Oreochromis niloticus species, weighing between 120 and 375 g, where chemical standards of water were included (T °, pH and OD), morphometry and haematological diagnosis, carried out at the Santo Domingo Experimental Farm from the University of the Amazonia, Florencia - Caquetá-Colombia. A directed and completely random sampling of 32 individuals was used, the blood was extracted by cardiac puncture and placed in Eppendorf tubes with EDTA. Blood smears were stained with Ema staining color for its laboratory analysis. The InfoStat was applied as a statistical method for the analysis of the variables to be measured. A total of leukocytes of 1.11 celx105 / mm3 was found, predominating neutrophils with 49.3% followed by lymphocytes with 38.8%, monocytes with 5.9%, eosinophils with 4.3%, basophils with 1.7 % and thrombocytes with 63.8% as an independent series. For the red series, an average of erythrocytes of 1.5 celx106 / mm3, hemoglobin of 7.9 g / dL; hematocrit of 23.3%, plasma proteins 3.3 g / dL, mean corpuscular volume 165 (u3), mean corpuscular hemoglobin 55 (uug) and mean corpuscular hemoglobin concentration of 33.3%. In conclusion, it was determined that some individuals presented Leukopenia with Lymphopenia and thrombocytosis. In addition, two individuals presented Normochromic Microcytic Anemia (AMN) and 19 individuals presented Normochromic Normocitic Anemia (ANN). The analysis of Total Plasma Proteins (PPT) determined that the high mortality rate was not due to nutritional deficiency. There was a positive similarity between the VCM and HCM, and negatively with the total number of erythrocytes.The hematological parameters were evaluated in the Oreochromis niloticus species, weighing between 120 and 375 g, where chemical standards of water were included (T °, pH and OD), morphometry and haematological diagnosis, carried out at the Santo Domingo Experimental Farm from the University of the Amazonia, Florencia - Caquetá-Colombia. A directed and completely random sampling of 32 individuals was used, the blood was extracted by cardiac puncture and placed in Eppendorf tubes with EDTA. Blood smears were stained with Ema staining color for its laboratory analysis. The InfoStat was applied as a statistical method for the analysis of the variables to be measured. A total of leukocytes of 1.11 celx105 / mm3 was found, predominating neutrophils with 49.3% followed by lymphocytes with 38.8%, monocytes with 5.9%, eosinophils with 4.3%, basophils with 1.7 % and thrombocytes with 63.8% as an independent series. For the red series, an average of erythrocytes of 1.5 celx106 / mm3, hemoglobin of 7.9 g / dL; hematocrit of 23.3%, plasma proteins 3.3 g / dL, mean corpuscular volume 165 (u3), mean corpuscular hemoglobin 55 (uug) and mean corpuscular hemoglobin concentration of 33.3%. In conclusion, it was determined that some individuals presented Leukopenia with Lymphopenia and thrombocytosis. In addition, two individuals presented Normochromic Microcytic Anemia (AMN) and 19 individuals presented Normochromic Normocitic Anemia (ANN). The analysis of Total Plasma Proteins (PPT) determined that the high mortality rate was not due to nutritional deficiency. There was a positive similarity between the VCM and HCM, and negatively with the total number of erythrocyte

Transforming European Water Governance? Participation and River Basin Management under the EU Water Framework Directive in 13 Member States

The European Union (EU) Water Framework Directive (WFD) requires EU member states to produce and implement river basin management plans, which are to be designed and updated via participatory processes that inform, consult with, and actively involve all interested stakeholders. The assumption of the European Commission is that stakeholder participation, and institutional adaptation and procedural innovation to facilitate it, are essential to the effectiveness of river basin planning and, ultimately, the environmental impact of the Directive. We analyzed official documents and the WFD literature to compare implementation of the Directive in EU member states in the initial WFD planning phase (2000–2009). Examining the development of participatory approaches to river basin management planning, we consider the extent of transformation in EU water governance over the period. Employing a mixed quantitative and qualitative approach, we map the implementation “trajectories” of 13 member states, and then provide a detailed examination of shifts in river basin planning and participation in four member states (Germany, Sweden, Poland and France) to illustrate the diversity of institutional approaches observed. We identify a general tendency towards increased, yet circumscribed, stakeholder participation in river basin management in the member states examined, alongside clear continuities in terms of their respective pre-WFD institutional and procedural arrangements. Overall, the WFD has driven a highly uneven shift to river basin-level planning among the member states, and instigated a range of efforts to institutionalize stakeholder involvement—often through the establishment of advisory groups to bring organized stakeholders into the planning process

Exploring the link between MORF4L1 and risk of breast cancer.

INTRODUCTION: Proteins encoded by Fanconi anemia (FA) and/or breast cancer (BrCa) susceptibility genes cooperate in a common DNA damage repair signaling pathway. To gain deeper insight into this pathway and its influence on cancer risk, we searched for novel components through protein physical interaction screens. METHODS: Protein physical interactions were screened using the yeast two-hybrid system. Co-affinity purifications and endogenous co-immunoprecipitation assays were performed to corroborate interactions. Biochemical and functional assays in human, mouse and Caenorhabditis elegans models were carried out to characterize pathway components. Thirteen FANCD2-monoubiquitinylation-positive FA cell lines excluded for genetic defects in the downstream pathway components and 300 familial BrCa patients negative for BRCA1/2 mutations were analyzed for genetic mutations. Common genetic variants were genotyped in 9,573 BRCA1/2 mutation carriers for associations with BrCa risk. RESULTS: A previously identified co-purifying protein with PALB2 was identified, MRG15 (MORF4L1 gene). Results in human, mouse and C. elegans models delineate molecular and functional relationships with BRCA2, PALB2, RAD51 and RPA1 that suggest a role for MRG15 in the repair of DNA double-strand breaks. Mrg15-deficient murine embryonic fibroblasts showed moderate sensitivity to γ-irradiation relative to controls and reduced formation of Rad51 nuclear foci. Examination of mutants of MRG15 and BRCA2 C. elegans orthologs revealed phenocopy by accumulation of RPA-1 (human RPA1) nuclear foci and aberrant chromosomal compactions in meiotic cells. However, no alterations or mutations were identified for MRG15/MORF4L1 in unclassified FA patients and BrCa familial cases. Finally, no significant associations between common MORF4L1 variants and BrCa risk for BRCA1 or BRCA2 mutation carriers were identified: rs7164529, Ptrend = 0.45 and 0.05, P2df = 0.51 and 0.14, respectively; and rs10519219, Ptrend = 0.92 and 0.72, P2df = 0.76 and 0.07, respectively. CONCLUSIONS: While the present study expands on the role of MRG15 in the control of genomic stability, weak associations cannot be ruled out for potential low-penetrance variants at MORF4L1 and BrCa risk among BRCA2 mutation carriers.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

Prospective individual patient data meta-analysis of two randomized trials on convalescent plasma for COVID-19 outpatients

Data on convalescent plasma (CP) treatment in COVID-19 outpatients are scarce. We aimed to assess whether CP administered during the first week of symptoms reduced the disease progression or risk of hospitalization of outpatients. Two multicenter, double-blind randomized trials (NCT04621123, NCT04589949) were merged with data pooling starting when = 50 years and symptomatic for <= 7days were included. The intervention consisted of 200-300mL of CP with a predefined minimum level of antibodies. Primary endpoints were a 5-point disease severity scale and a composite of hospitalization or death by 28 days. Amongst the 797 patients included, 390 received CP and 392 placebo; they had a median age of 58 years, 1 comorbidity, 5 days symptoms and 93% had negative IgG antibody-test. Seventy-four patients were hospitalized, 6 required mechanical ventilation and 3 died. The odds ratio (OR) of CP for improved disease severity scale was 0.936 (credible interval (CI) 0.667-1.311); OR for hospitalization or death was 0.919 (CI 0.592-1.416). CP effect on hospital admission or death was largest in patients with <= 5 days of symptoms (OR 0.658, 95%CI 0.394-1.085). CP did not decrease the time to full symptom resolution

Diseño y manufactura de pie Protésico

El término prótesis hace referencia al dispositivo que sustituye o compensa la pérdida de un miembro perdido, para el caso objeto de estudio, se da en una prótesis de miembro inferior, en el caso de una amputación de miembros inferiores a nivel transtibial, el pie es el elemento ausente más significativo ya que asume la estabilidad mínima del cuerpo del paciente, además de interferir en el balance intrínseco del cuerpo humano. Por lo anteriormente descrito uno de los pilares más importantes de la investigación consiste en el desarrollo y ensamble de un pie protésico que cumpla con las características morfológicas, físicas y mecánicas de pie a sustituir en las prótesis propias de nuestros pacientes caso de estudio

Knowledge synthesis for environmental decisions : an evaluation of existing methods, and guidance for their selection, use and development : a report from the EKLIPSE project

Environmental governance in context: a study of process dynamics, contextual features and outcomes in four empirical case