60 research outputs found
The relationship between prior night's sleep and measures of infant imitation.
We examined whether sleep quality during the night and naps during the day preceding a learning event are related to memory encoding in human infants. Twenty-four 6- and twenty-four 12-month-old infants' natural sleeping behavior was monitored for 24âhr using actigraphy. After the recording period, encoding was assessed using an imitation paradigm. In an initial baseline phase, infants were allowed to interact with the stimulus to assess spontaneous production of any target actions. Infants then watched an experimenter demonstrate a sequence of three target actions and were immediately given the opportunity to reproduce the demonstrated target actions to assess memory encoding. Analyses revealed significant correlations between nighttime sleep quality variables (sleep efficiency, sleep fragmentation) and immediate imitation in 6-month-olds, but not in 12-month-olds. High sleep quality in the preceding night was thus positively associated with next day's memory encoding in 6-month-old infants. © 2016 Wiley Periodicals, Inc. Dev Psychobiol 9999: 1-12, 2016
On-Chip Integration of Functional Hybrid Materials and Components in Nanophotonics and Optoelectronics
[No abstract available
Recommended from our members
Surfactant-Free dâLimonene Encapsulation in Spray-Dried Alginate Microcapsules Cross-Linked by In Situ Internal Gelation
Microencapsulation facilitates incorporating bioactive volatile compounds into products throughout the food, health, and cosmetics industries. To minimize the number of ingredients when microencapsulating volatile oils, we examined surfactant-free encapsulation of d-limonene in cross-linked alginate microcapsules (CLAMs) via in situ cross-linking during spray drying. Surfactant-free CLAMs (SF-CLAMs) were prepared by forming a Pickering d-limonene emulsion stabilized by calcium carbonate nanoparticles (CaCO3-NPs), combining with alginates, and then spray drying. CaCO3-NPs served as both the emulsifier and the reservoir of alginate cross-linking agent. SF-CLAMs, with a volatile retention of 73.1 ± 4.7% and total limonene content of 13.6 ± 8.6% (w/w, d.b.), exhibited core-shell morphology where CaCO3-NPs surrounded large emulsion cores (âŒ5 ÎŒm) encased in densely cross-linked alginate shells. Limonene was fully retained for up to 4 h in SF-CLAMs in water at 37 °C. Moreover, microencapsulation in SF-CLAMs minimized release in simulated gastric fluid (2.2 ± 0.3% in 2 h) while fully releasing in simulated gastric fluid at 37 °C
Comparative Technoeconomic Process Analysis of Industrial-Scale Microencapsulation of Bioactives in Cross-Linked Alginate
The food, chemical, and biotechnology industries offer many potential applications for calcium alginate microencapsulation, but this technique is largely confined to the laboratory bench due to scalability challenges. Scaling up the traditional external gelation method requires several costly unit operations. Alternatively, a consolidated process accomplishes alginate cross-linking in situ during spray-drying to form cross-linked alginate microcapsules (âthe CLAMs processâ). This work examined the process economics of these two microencapsulation processes through technoeconomic analysis. Parallel batch process models were constructed in SuperPro Designer, initially for encapsulating emulsified fish oil. At all production scales examined, the capital investment and annual operating cost were lower for the CLAMs process. Modifying the external gelation process marginally improved the process economics, but costs remained elevated. The CLAMs processâ economic advantage stemmed from reducing the number of unit procedures, which lowered the equipment purchase cost and the dependent components of capital investment and annual operating cost. Upon modifying the models for microencapsulating hydrophilic cargo (e.g. enzymes, vitamins, microbial concentrates), the CLAMs process remained favorable at all cargo material costs and cargo loadings examined. This work demonstrates the utility of technoeconomic analysis for evaluating microencapsulation processes and may justify applying the CLAMs process at the industrial scale. </div
Recommended from our members
Chelator Regulation of In Situ Calcium Availability to Enable Spray-Dry Microencapsulation in Cross-Linked Alginates.
A recently patented one-step in situ cross-linked alginate microencapsulation (CLAM) by spray-drying (i.e., the UC Davis CLAMs technology) can overcome the high cost of scale-up that limits commercial applications. While increasing calcium loading in the CLAMs process can increase the extent of cross-linking and improve retention and protection of the encapsulated cargo, the potential for residual undissolved calcium salt crystals in the final product can be a concern for some applications. Here, we demonstrate an alternate one-step spray-dry CLAMs process using pH-responsive chelation of calcium. The "Chelate CLAMs" process is an improvement over the patented process that controls ion availability based on pH-responsive solubility of the calcium salt. Hyaluronic acid was encapsulated in CLAMs to minimize swelling and release in aqueous formulations. CLAMs with 61% (d.b.) hyaluronic acid (HA-CLAMs) demonstrated restricted plumping, limited water absorption capacity, and reduced leaching, retaining up to 49% hyaluronic acid after 2 h in water. Alternatively, "Chelate HA-CLAMs" formed by the improved process exhibited nearly full retention of hyaluronic acid over 2 h in water and remained visibly insoluble after 1 year of storage in water at 4 °C. Successful hyaluronic acid retention in CLAMs is likely due in part to its ability to cross-link with calcium
Recommended from our members
Industrially-Scalable Microencapsulation of Plant Beneficial Bacteria in Dry Cross-Linked Alginate Matrix.
Microencapsulation of plant-beneficial bacteria, such as pink pigmented facultative methylotrophs (PPFM), may greatly extend the shelf life of these Gram-negative microorganisms and facilitate their application to crops for sustainable agriculture. A species of PPFM designated Methylobacterium radiotolerans was microencapsulated in cross-linked alginate microcapsules (CLAMs) prepared by an innovative and industrially scalable process that achieves polymer cross-linking during spray-drying. PPFM survived the spray-drying microencapsulation process with no significant loss in viable population, and the initial population of PPFM in CLAMs exceeded 1010 CFU/g powder. The PPFM population in CLAMs gradually declined by 4 to 5 log CFU/g over one year of storage. The extent of alginate cross-linking, modulated by adjusting the calcium phosphate content in the spray-dryer feed, did not influence cell viability after spray-drying, viability over storage, or dry particle size. However, particle size measurements and light microscopy of aqueous CLAMs suggest that enhanced crosslinking may limit the release of encapsulated bacteria. This work demonstrates an industrially scalable method for producing alginate-based inoculants that may be suitable for on-seed or foliar spray applications
How alginate properties influence in situ internal gelation in Crosslinked Alginate Microcapsules (CLAMs) formed by spray drying
Alginates gel rapidly under ambient conditions and have
widely documented potential to form protective matrices for sensitive bioactive
cargo. Most commonly, alginate gelation occurs via calcium mediated
electrostatic crosslinks between the linear polyuronic acid polymers. A recent
breakthrough to form crosslinked alginate microcapsules (CLAMs) by in situ
gelation during spray drying (âCLAMs processâ) has demonstrated applications in
protection and controlled delivery of bioactives in food, cosmetics, and
agriculture. The extent of crosslinking of alginates in CLAMs impacts the
effectiveness of its barrier properties. For example, higher crosslinking
extents can improve oxidative stability and limit diffusion of the encapsulated
cargo. Crosslinking in CLAMs can be controlled by varying the calcium to
alginate ratio; however, the choice of alginates used in the process also influences
the ultimate extent of crosslinking. To understand how to select alginates to
target crosslinking in CLAMs, we examined the roles of alginate molecular properties.
A surprise finding was the formation of alginic acid in the CLAMs that is a
consequence of simultaneous and rapid pH reduction and moisture removal that occurs
during spray drying. Thus, spray dried CLAMs gelation is due to calcium
crosslinking and alginic acid formation, and unlike external gelation methods, is
insensitive to the molecular composition of the alginates. The âextent of
gelationâ of spray dried CLAMs is influenced by the molecular weights of the
alginates at saturating calcium concentrations. Alginate viscosity correlates
with molecular weight; thus, viscosity is a convenient criterion for selecting
commercial alginates to target gelation extent in CLAMs.</p
- âŠ