Evolution-guided functional analyses reveal diverse antiviral specificities encoded by IFIT1 genes in mammals.

IFIT (interferon-induced with tetratricopeptide repeats) proteins are critical mediators of mammalian innate antiviral immunity. Mouse IFIT1 selectively inhibits viruses that lack 2'O-methylation of their mRNA 5' caps. Surprisingly, human IFIT1 does not share this antiviral specificity. Here, we resolve this discrepancy by demonstrating that human and mouse IFIT1 have evolved distinct functions using a combination of evolutionary, genetic and virological analyses. First, we show that human IFIT1 and mouse IFIT1 (renamed IFIT1B) are not orthologs, but are paralogs that diverged >100 mya. Second, using a yeast genetic assay, we show that IFIT1 and IFIT1B proteins differ in their ability to be suppressed by a cap 2'O-methyltransferase. Finally, we demonstrate that IFIT1 and IFIT1B have divergent antiviral specificities, including the discovery that only IFIT1 proteins inhibit a virus encoding a cap 2'O-methyltransferase. These functional data, combined with widespread turnover of mammalian IFIT genes, reveal dramatic species-specific differences in IFIT-mediated antiviral repertoires

Binding and Relocalization of PKR 1 by Murine Cytomegalovirus

Many viruses have evolved mechanisms to evade the repression of translation mediated by protein kinase R (PKR). In the case of murine cytomegalovirus (MCMV), the protein products of two essential genes, m142 and m143, bind to dsRNA and block phosphorylation of PKR and eukaryotic initiation factor 2. A distinctive feature of MCMV is that two proteins are required to block PKR activation whereas other viral dsRNA-binding proteins that prevent PKR activation contain all the necessary functions in a single protein. In order to better understand the mechanism by which MCMV evades the PKR response, we investigated the associations of pm142 and pm143 with each other and with PKR. Both pm142 and pm143 interact with PKR in infected and transfected cells. However, the ~200 kDa pm142:pm143 complex that forms in these cells does not contain substantial amounts of PKR, suggesting that the interactions between pm142:pm143 and PKR are unstable or transient. The stable, soluble pm142:pm143 complex appears to be a heterotetramer consisting of two molecules of pm142 associated with each other and each one binding to and stabilizing a monomer of pm143. MCMV infection also causes relocalization of PKR into the nucleus and to an insoluble cytoplasmic compartment. These results suggest a model in which the pm142:pm143 multimer interacts with PKR and causes its sequestration in cellular compartments where it is unable to shut off translation and repress viral replication

Humoral immune response to proteins of human cytomegalovirus latency-associated transcripts

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ULAS J141623.94+134836.3: a Blue T Dwarf Companion to a Blue L Dwarf

We confirm the substellar nature of ULAS J141623.94+134836.3, a common proper motion companion to the blue L dwarf SDSS J141624.08+134826.7 identified by Burningham et al. and Scholz. Low-resolution 0.8-2.4 micron spectroscopy obtained with IRTF/SpeX shows strong H2O and CH4 absorption bands, consistent with a T7.5 spectral type, and we see possible indications of NH3 absorption in the 1.0-1.3 micron region. More importantly, the spectrum of ULAS J1416+1348 shows a broadened Y-band peak and highly suppressed K-band flux, both indicative of high surface gravity and/or subsolar metallicity. These traits are verified through spectral model fits, from which we derive atmospheric parameters Teff = 650+/-60 K, log g = 5.2+/-0.4 cgs, [M/H] <= -0.3 and Kzz = 10^4 cm^2/s, the temperature being significantly warmer than that estimated by Burningham et al. These fits also indicate a model-dependent spectroscopic distance of 10.6(+3.0,-2.8) pc for ULAS J1416+1348, formally consistent with the 7.9+/-1.7 pc astrometric distance for SDSS J1416+1348 from Scholz. The common peculiarities of these two co-spatial, co-moving sources suggest that their unusual blue colors - and those of other blue L and T dwarfs in general - arise from age or metallicity, rather than cloud properties alone.Comment: 21 pages, 3 figures (manuscript format); submitted to ApJ, constructive comments welcom

Discovery of An Unusually Blue L Dwarf Within 10 pc of the Sun

We report the discovery of an unusually blue L5 dwarf within 10 pc of the Sun from a search of Sloan Digital Sky Survey (SDSS) spectra. A spectrophotometric distance estimate of 8.0+/-1.6 pc places SDSS J141624.08+134826.7 among the six closest known L dwarfs. SDSS 1416+13 was overlooked in infrared color-based searches because of its unusually blue J-K_S color, which also identifies it as the nearest member of the blue L dwarf subclass. We present additional infrared and optical spectroscopy from the IRTF/SpeX and Magellan/MagE spectrographs and determine UVW motions that indicate thin disk kinematics. The inclusion of SDSS 1416+13 in the 20 pc sample of L dwarfs increases the number of L5 dwarfs by 20% suggesting that the L dwarf luminosity function may be far from complete.Comment: 15 pages, 3 figures, accepted for publication in AJ; updated version includes corrected radial velocit

WISEP J180026.60+013453.1: A Nearby Late L Dwarf Near the Galactic Plane

We report a nearby L7.5 dwarf discovered using the Preliminary Data Release of the Wide-field Infrared Survey Explorer (WISE) and the Two Micron All-Sky Survey (2MASS). WISEP J180026.60+013453.1 has a motion of 0.42 arcsec/yr and an estimated distance of 8.8 \pm 1.0 pc. With this distance, it currently ranks as the sixth closest known L dwarf, although a trigonometric parallax is needed to confirm this distance. It was previously overlooked because it lies near the Galactic Plane (b=12). As a relatively bright and nearby late L dwarf with normal near-infrared colors, W1800+0134 will serve as a benchmark for studies of cloud-related phenomena in cool substellar atmospheres.Comment: 12 pages, 2 figure, accepted to the Astronomical Journal (AJ

Atmospheric circulation of hot Jupiters: Coupled radiative-dynamical general circulation model simulations of HD 189733b and HD 209458b

We present global, three-dimensional numerical simulations of HD 189733b and HD 209458b that couple the atmospheric dynamics to a realistic representation of non-gray cloud-free radiative transfer. The model, which we call the Substellar and Planetary Atmospheric Radiation and Circulation (SPARC) model, adopts the MITgcm for the dynamics and uses the radiative model of McKay, Marley, Fortney, and collaborators for the radiation. Like earlier work with simplified forcing, our simulations develop a broad eastward equatorial jet, mean westward flow at higher latitudes, and substantial flow over the poles at low pressure. For HD 189733b, our simulations without TiO and VO opacity can explain the broad features of the observed 8 and 24-micron light curves, including the modest day-night flux variation and the fact that the planet/star flux ratio peaks before the secondary eclipse. Our simulations also provide reasonable matches to the Spitzer secondary-eclipse depths at 4.5, 5.8, 8, 16, and 24 microns and the groundbased upper limit at 2.2 microns. However, we substantially underpredict the 3.6-micron secondary-eclipse depth, suggesting that our simulations are too cold in the 0.1-1 bar region. Predicted temporal variability in secondary-eclipse depths is ~1% at Spitzer bandpasses, consistent with recent observational upper limits at 8 microns. We also show that nonsynchronous rotation can significantly alter the jet structure. For HD 209458b, we include TiO and VO opacity; these simulations develop a hot (>2000 K) dayside stratosphere. Despite this stratosphere, we do not reproduce current Spitzer photometry of this planet. Light curves in Spitzer bandpasses show modest phase variation and satisfy the observational upper limit on day-night phase variation at 8 microns. (abridged)Comment: 20 pages (emulate-apj format), 21 figures, final version now published in ApJ. Includes expanded discussion of radiative-transfer methods and two new figure

Resolved Spectroscopy of M Dwarf/L Dwarf Binaries. IV. Discovery of an M9 + L6 BInary Separated by Over 100 AU

We report the discovery of a faint L6 \pm 1 companion to the previously known M9 dwarf, 2MASS J01303563-4445411, based on our near-infrared imaging and spectroscopic observations with the 3m Infrared Telescope Facility SpeX imager/spectrometer. The visual binary is separated by 3. 28 \pm 0. 05 on the sky at a spectrophotometric distance of 40 \pm 14 pc. The projected physical separation is 130 \pm 50 AU, making it one of the widest VLM field multiples containing a brown dwarf companion. 2MASS J0130-4445 is only one of ten wide VLM pairs and only one of six in the field. The secondary is considerably fainter ({\Delta}K ~ 2.35 mag) and redder ({\Delta} (J - Ks) ~ 0.81 dex), consistent with component near-infrared types of M9.0 \pm 0.5 and L6 \pm 1 based on our resolved spectroscopy. The component types suggest a secondary mass well within the hydrogen-burning limit and an age-dependent mass ratio of 0.6-0.9. The system's space motion and spectroscopic indicators suggest an age of 2-4 Gyr while the model-dependent masses and binding energies suggest that this system is unlikely to have formed via dynamical ejection. The age, composition, and separation of the 2MASS J01303563-4445411 system make it useful for tests of VLM formation theories and of condensate cloud formation in L dwarfs.Comment: Accepted by the AJ (8 pages, emulateapj format

Clouds in the Coldest Brown Dwarfs: FIRE Spectroscopy of Ross 458C

Condensate clouds are a salient feature of L dwarf atmospheres, but have been assumed to play little role in shaping the spectra of the coldest T-type brown dwarfs. Here we report evidence of condensate opacity in the near-infrared spectrum of the brown dwarf candidate Ross 458C, obtained with the Folded-Port Infrared Echellette (FIRE) spectrograph at the Magellan Telescopes. These data verify the low-temperature nature of this source, indicating a T8 spectral classification, log Lbol/Lsun = -5.62+/-0.03, Teff = 650+/-25 K, and a mass at or below the deuterium burning limit. The data also reveal enhanced emission at K-band associated with youth (low surface gravity) and supersolar metallicity, reflecting the properties of the Ross 458 system (age = 150-800 Myr, [Fe/H] = +0.2 to +0.3). We present fits of FIRE data for Ross 458C, the T9 dwarf ULAS J133553.45+113005.2, and the blue T7.5 dwarf SDSS J141624.08+134826.7B, to cloudless and cloudy spectral models from Saumon & Marley. For Ross 458C we confirm a low surface gravity and supersolar metallicity, while the temperature differs depending on the presence (635 [+25,-35] K) or absence (760 [+70,-45] K) of cloud extinction. ULAS J1335+1130 and SDSS J1416+1348B have similar temperatures (595 [+25,-45] K), but distinct surface gravities (log g = 4.0-4.5 cgs versus 5.0-5.5 cgs) and metallicities ([M/H] ~ +0.2 versus -0.2). In all three cases, cloudy models provide better fits to the spectral data, significantly so for Ross 458C. These results indicate that clouds are an important opacity source in the spectra of young cold T dwarfs, and should be considered when characterizing the spectra of planetary-mass objects in young clusters and directly-imaged exoplanets. The characteristics of Ross 458C suggest it could itself be regarded as a planet, albeit one whose cosmogony does not conform with current planet formation theories.Comment: Accepted for publication to ApJ: 18 pages, 11 figures in emulateapj forma

A Disk Around the Planetary-Mass Companion GSC 06214-00210 b: Clues About the Formation of Gas Giants on Wide Orbits

We present Keck/OSIRIS 1.1-1.8 um adaptive optics integral field spectroscopy of the planetary-mass companion to GSC 06214-00210, a member of the ~5 Myr Upper Scorpius OB association. We infer a spectral type of L0+/-1, and our spectrum exhibits multiple signs of youth. The most notable feature is exceptionally strong PaBeta emission (EW=-11.4 +/- 0.3 A) which signals the presence of a circumplanetary accretion disk. The luminosity of GSC 06214-00210 b combined with its age yields a model-dependent mass of 14 +/- 2 MJup, making it the lowest-mass companion to show evidence of a disk. With a projected separation of 320 AU, the formation of GSC 06214-00210 b and other very low-mass companions on similarly wide orbits is unclear. One proposed mechanism is formation at close separations followed by planet-planet scattering to much larger orbits. Since that scenario involves a close encounter with another massive body, which is probably destructive to circumplanetary disks, it is unlikely that GSC 06214-00210 b underwent a scattering event in the past. This implies that planet-planet scattering is not solely responsible for the population of gas giants on wide orbits. More generally, the identification of disks around young planetary companions on wide orbits offers a novel method to constrain the formation pathway of these objects, which is otherwise notoriously difficult to do for individual systems. We also refine the spectral type of the primary from M1 to K7 and detect a mild (2-sigma) excess at 22 um using WISE photometry.Comment: 25 pages, 13 figures; Accepted by Ap