646 research outputs found
A combined analysis technique for the search for fast magnetic monopoles with the MACRO detector
We describe a search method for fast moving ()
magnetic monopoles using simultaneously the scintillator, streamer tube and
track-etch subdetectors of the MACRO apparatus. The first two subdetectors are
used primarily for the identification of candidates while the track-etch one is
used as the final tool for their rejection or confirmation. Using this
technique, a first sample of more than two years of data has been analyzed
without any evidence of a magnetic monopole. We set a 90% CL upper limit to the
local monopole flux of in the
velocity range and for nucleon decay
catalysis cross section smaller than .Comment: 29 pages (12 figures). Accepted by Astroparticle Physic
Final results of magnetic monopole searches with the MACRO experiment
We present the final results obtained by the MACRO experiment in the search
for GUT magnetic monopoles in the penetrating cosmic radiation, for the range
. Several searches with all the MACRO sub-detectors
(i.e. scintillation counters, limited streamer tubes and nuclear track
detectors) were performed, both in stand alone and combined ways. No candidates
were detected and a 90% Confidence Level (C.L.) upper limit to the local
magnetic monopole flux was set at the level of cm
s sr. This result is the first experimental limit obtained in
direct searches which is well below the Parker bound in the whole range
in which GUT magnetic monopoles are expected.Comment: 12 pages, Latex, 9 figures and 2 Table
The Observation of Up-going Charged Particles Produced by High Energy Muons in Underground Detectors
An experimental study of the production of up-going charged particles in
inelastic interactions of down-going underground muons is reported, using data
obtained from the MACRO detector at the Gran Sasso Laboratory. In a sample of
12.2 10^6 single muons, corresponding to a detector livetime of 1.55 y, 243
events are observed having an up-going particle associated with a down-going
muon. These events are analysed to determine the range and emission angle
distributions of the up-going particle, corrected for detection and
reconstruction efficiency. Measurements of the muon neutrino flux by
underground detectors are often based on the observation of through-going and
stopping muons produced in interactions in the rock below the
detector. Up-going particles produced by an undetected down-going muon are a
potential background source in these measurements. The implications of this
background for neutrino studies using MACRO are discussed.Comment: 18 pages, 9 figures. Accepted by Astrop. Physic
Nuclearite search with the MACRO detector at Gran Sasso
In this paper we present the results of a search for nuclearites in the
penetrating cosmic radiation using the scintillator and track-etch subdetectors
of the MACRO apparatus. The analyses cover the beta =v/c range at the detector
depth (3700 hg/cm^2) 10^-5 < beta < 1; for beta = 2 x 10^-3 the flux limit is
2.7 x 10^-16 cm^-2 s^-1 sr^-1 for an isotropic flux of nuclearites, and twice
this value for a flux of downgoing nuclearites.Comment: 16 pages, 4 Encapsulated Postscript figures, uses article.sty.
Submitted to The European Physical Journal
Atmospheric neutrino induced muons in the MACRO detector
A measurement of the flux of neutrino-induced muons using the MACRO detector
is presented. Different event topologies, corresponding to different neutrino
parent energies can be detected. The upward throughgoing muon sample is the
larger event sample. The observed upward-throughgoing muons are 26% fewer than
expected and the zenith angle distribution does not fit with the expected one.
Assuming neutrino oscillations, both measurements suggest maximum mixing and
Dm2 of a few times 10-3 eV2. The other samples are due to the internally
produced events and to upward-going stopping muons. These data show a regular
deficit of observed events in each angular bin, as expected assuming neutrino
oscillations with maximum mixing, in agreement with the analysis of the
upward-throughgoing muon sample.Comment: 7 pages 6 figures to appear in the proceedings of XVIII International
Conference on Neutrino Physics and Astrophysics (Neutrino'98), Takayama,
Japan 4-9 June, 199
New MACRO results on atmospheric neutrino oscillations
The final results of the MACRO experiment on atmospheric neutrino
oscillations are presented and discussed. The data concern different event
topologies with average neutrino energies of ~3 and ~50 GeV. Multiple Coulomb
Scattering of the high energy muons in absorbers was used to estimate the
neutrino energy of each event. The angular distributions, the L/E_nu
distribution, the particle ratios and the absolute fluxes all favour nu_mu -->
nu_tau oscillations with maximal mixing and Delta m^2 =0.0023 eV^2. A
discussion is made on the Monte Carlos used for the atmospheric neutrino flux.
Some results on neutrino astrophysics are also briefly discussed.Comment: Invited Paper at the NANP03 Int. Conf., Dubna, 200
Observation of associated near-side and away-side long-range correlations in √sNN=5.02 TeV proton-lead collisions with the ATLAS detector
Two-particle correlations in relative azimuthal angle (Δϕ) and pseudorapidity (Δη) are measured in √sNN=5.02 TeV p+Pb collisions using the ATLAS detector at the LHC. The measurements are performed using approximately 1 μb-1 of data as a function of transverse momentum (pT) and the transverse energy (ΣETPb) summed over 3.1<η<4.9 in the direction of the Pb beam. The correlation function, constructed from charged particles, exhibits a long-range (2<|Δη|<5) “near-side” (Δϕ∼0) correlation that grows rapidly with increasing ΣETPb. A long-range “away-side” (Δϕ∼π) correlation, obtained by subtracting the expected contributions from recoiling dijets and other sources estimated using events with small ΣETPb, is found to match the near-side correlation in magnitude, shape (in Δη and Δϕ) and ΣETPb dependence. The resultant Δϕ correlation is approximately symmetric about π/2, and is consistent with a dominant cos2Δϕ modulation for all ΣETPb ranges and particle pT
Virulence Regulator EspR of Mycobacterium tuberculosis Is a Nucleoid-Associated Protein
The principal virulence determinant of Mycobacterium tuberculosis (Mtb), the ESX-1 protein secretion system, is positively controlled at the transcriptional level by EspR. Depletion of EspR reportedly affects a small number of genes, both positively or negatively, including a key ESX-1 component, the espACD operon. EspR is also thought to be an ESX-1 substrate. Using EspR-specific antibodies in ChIP-Seq experiments (chromatin immunoprecipitation followed by ultra-high throughput DNA sequencing) we show that EspR binds to at least 165 loci on the Mtb genome. Included in the EspR regulon are genes encoding not only EspA, but also EspR itself, the ESX-2 and ESX-5 systems, a host of diverse cell wall functions, such as production of the complex lipid PDIM (phenolthiocerol dimycocerosate) and the PE/PPE cell-surface proteins. EspR binding sites are not restricted to promoter regions and can be clustered. This suggests that rather than functioning as a classical regulatory protein EspR acts globally as a nucleoid-associated protein capable of long-range interactions consistent with a recently established structural model. EspR expression was shown to be growth phase-dependent, peaking in the stationary phase. Overexpression in Mtb strain H37Rv revealed that EspR influences target gene expression both positively or negatively leading to growth arrest. At no stage was EspR secreted into the culture filtrate. Thus, rather than serving as a specific activator of a virulence locus, EspR is a novel nucleoid-associated protein, with both architectural and regulatory roles, that impacts cell wall functions and pathogenesis through multiple genes
Simultaneous Analysis of Multiple Mycobacterium tuberculosis Knockdown Mutants In Vitro and In Vivo
Mycobacterium tuberculosis (Mtb) represents one of the most persistent bacterial threats to human health and new drugs are needed to limit its impact. Conditional knockdown mutants can help validate new drug targets, but the analysis of individual mutants is laborious and time consuming. Here, we describe quantitative DNA tags (qTags) and their use to simultaneously analyze conditional Mtb knockdown mutants that allowed silencing the glyoxylate and methylcitrate cycles (via depletion of isocitrate lyase, ICL), the serine protease Rv3671c, and the core subunits of the mycobacterial proteasome, PrcB and PrcA. The impact of gene silencing in multi-strain cultures was determined by measuring the relative abundance of mutant-specific qTags with real-time PCR. This achieved accurate quantification over a broad range of qTag abundances and depletion of ICL, Rv3671c, or PrcBA resulted in the expected impairment of growth of Mtb with butyrate as the primary carbon source, survival during oxidative stress, acid stress and starvation. The impact of depleting ICL, Rv3671c, or PrcBA in multi-strain mouse infections was analyzed with two approaches. We first measured the relative abundance of mutant-specific qTags in total chromosomal DNA isolated from bacteria that were recovered from infected lungs on agar plates. We then developed a two-step amplification procedure, which allowed us to measure the abundances of individual mutants directly in infected lung tissue. Both strategies confirmed that inactivation of Rv3671c and PrcBA severely reduced persistence of Mtb in mice. The multi-strain infections furthermore suggested that silencing ICL not only prevented growth of Mtb during acute infections but also prevented survival of Mtb during chronic infections. Analyses of the ICL knockdown mutant in single-strain infections confirmed this and demonstrated that silencing of ICL during chronic infections impaired persistence of Mtb to the extent that the pathogen was cleared from the lungs of most mice
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