Assessment of Nutritional Status in Children from Eastern Sudan

Background: Malnutrition is a very important risk factor leading illness and death in children worldwide.Objective: The aim of this study was to assess the nutritional status and relevant haematological and biochemical parameters in school children.Materials and Methods: Cross sectional study, was conducted in 120 (70 boys and 50 girls) school going children of 6-7 years of age, for the assessment of their nutritional status. The haemogloblin concentration (Hb%) was measured by equation method, packed cell volume (PCV) was estimated by scale of microhameatocrit reader, and mean corpuscular hemoglobin concentration (MCHC) was calculated. Serum total protein, albumin, iron, total iron binding capacity and transferrin were measured by colorimetric methods. Weight (kg) and height (cm) were measured and body mass index was calculated. Data were analyzed using SPSS version13.0.Results: The mean values for hematological, biochemical and  anthropometrical measurements were much below the normal ranges. The anthropometric percentile measured in the children showed malnutrition cases in 32 (26.7%) and malnutrition and underweight in 73 (60.8%) while the body mass index showed underweight in males in 23 (19.2%) and in females 11 (9.2%).Conclusions: Malnutrition is common in our study population and was seen in 48.3 of children. It was accompanied by anaemia in 60.8 % of children.Key words: Biochemical parameters, Hematological parameters,  Anthropometric measurements, Nutritional status, Anemia

Phagocytosis-dependent activation of a TLR9-BTK-calcineurin-NFAT pathway co-ordinates innate immunity to Aspergillus fumigatus.

Transplant recipients on calcineurin inhibitors are at high risk of invasive fungal infection. Understanding how calcineurin inhibitors impair fungal immunity is a key priority for defining risk of infection. Here, we show that the calcineurin inhibitor tacrolimus impairs clearance of the major mould pathogen Aspergillus fumigatus from the airway, by inhibiting macrophage inflammatory responses. This leads to defective early neutrophil recruitment and fungal clearance. We confirm these findings in zebrafish, showing an evolutionarily conserved role for calcineurin signalling in neutrophil recruitment during inflammation. We find that calcineurin-NFAT activation is phagocytosis dependent and collaborates with NF-κB for TNF-α production. For yeast zymosan particles, activation of macrophage calcineurin-NFAT occurs via the phagocytic Dectin-1-spleen tyrosine kinase pathway, but for A. fumigatus, activation occurs via a phagosomal TLR9-dependent and Bruton's tyrosine kinase-dependent signalling pathway that is independent of MyD88. We confirm the collaboration between NFAT and NF-κB for TNF-α production in primary alveolar macrophages. These observations identify inhibition of a newly discovered macrophage TLR9-BTK-calcineurin-NFAT signalling pathway as a key immune defect that leads to organ transplant-related invasive aspergillosis