On the use of Ajisai and Jason-1 satellites for tests of General Relativity

Here we analyze in detail some aspects of the proposed use of Ajisai and Jason-1, together with the LAGEOS satellites, to measure the general relativistic Lense-Thirring effect in the gravitational field of the Earth. A linear combination of the nodes of such satellites is the proposed observable. The systematic error due to the mismodelling in the uncancelled even zonal harmonics would be \sim 1% according to the latest present-day CHAMP/GRACE-based Earth gravity models. In regard to the non-gravitational perturbations especially affecting Jason-1, only relatively high-frequency harmonic perturbations should occur: neither semisecular nor secular bias of non-gravitational origin should affect the proposed combination: their maximum impact is evaluated to \sim 4% over 2 years. Our estimation of the root-sum-square total error is about 4-5% over at least 3 years of data analysis required to average out the uncancelled tidal perturbations.Comment: Latex, 24 pages, 5 tables, 1 figure. Two references added, minor modifications. To appear in New Astronom

Pharmacokinetics, pharmacodynamics, and safety of moss-aGalactosidase A in patients with Fabry disease

Moss-aGalactosidase A (moss-aGal) is a moss-derived version of human α-galactosidase developed for enzyme replacement therapy in patients with Fabry disease. It exhibits a homogenous N-glycosylation profile with >90% mannose-terminated glycans. In contrast to mammalian cell produced α-galactosidase, moss-aGal does not rely on mannose-6-phosphate receptor mediated endocytosis but targets the mannose receptor for tissue uptake. We conducted a phase 1 clinical trial with moss-aGal in six patients with confirmed diagnosis of Fabry disease during a 28-day schedule. All patients received a single dose of 0.2 mg/kg moss-aGal by i.v.-infusion. Primary endpoints of the trial were safety and pharmacokinetics; secondary endpoints were pharmacodynamics by analyzing urine and plasma Gb3 and lyso-Gb3 concentrations. In all patients, the administered single dose was well tolerated. No safety issues were observed. Pharmacokinetic data revealed a stable nonlinear profile with a short plasma half-life of moss-aGal of 14 minutes. After one single dose of moss-aGal, urinary Gb3 concentrations decreased up to 23% 7 days and up to 60% 28 days post-dose. Plasma concentrations of lyso-Gb3 decreased by 3.8% and of Gb3 by 11% 28 days post-dose. These data reveal that a single dose of moss-aGal was safe, well tolerated, and led to a prolonged reduction of Gb3 excretion. As previously shown, moss-aGal is taken up via the mannose receptor, which is expressed on macrophages but also on endothelial and kidney cells. Thus, these data indicate that moss-aGal may target kidney cells. After these promising results, phase 2/3 clinical trials are in preparation

Penguatan Kapasitas Petani Kopi Gunung Wayang Menuju Pengembangan Wisata Desa Sumberwuluh Melalui Metode CBPR

Gunung Wayang Tourism Development through coffee farmers in order to realize the optimization of the potential in Mount Wayang Sumberwuluh Village Candipuro District Lumajang Regency with Community based participatori researchMethod. The process of utilizing local natural resources from coffee farming as one of the development of the enthusiasm of local residents to make Sumberwuluh Village a tourist village. The results of the study were conducted through several processes of empowering coffee farmers in Sumberwuluh Village.The process of empowering coffee farmers continues in three stages, namely the implementation of inculturation, assistance and strengthening the capacity of coffee farmers. This study uses the CBPR method, research with several stages in the field starting from laying the foundation, research planning, gathering and acting on findings. Steps are taken to start deeper into the life and potential that exists in Sumberwuluh Village. Strategies in developing coffee capacity, by providing coffee in the form of ground coffee and coffee muffins. In this case, cooperation with village institutions is urgently needed. The results of this empowerment of coffee farmers, support to add to the aesthetic value of packaging coffee and processed coffee products with a modern touch as a souvenir in Gunung Wayang Tourism which is a product of Sumberwuluh Village. Forms of farmer empowerment, starting from planning, implementation and evaluation. Related to the challenges in empowering coffee farmers related to efforts to maintain old habits that have been implemented by coffee farmers before this innovation, the attitude of people who need can not be supported and try new things, the limited need for tourism assistance while improving promotional activities. Community empowerment through village development has implications for socio-cultural resilience including strengthening and various changes in social, cultural and environmental values. Keywords: Empowerment of Coffee Farmers; Gunung Wayang; Tourism Village Development Abstrak Pengembangan Wisata Gunung Wayang melalui optimalisasi potensi ekonomi petani kopi diDesa Sumberwuluh Kecamatan Candipuro Kabupaten Lumajang melalui metode Community based participatori research. Proses pemanfaatan sumber daya alam lokal daripertanian kopi sebagai salah satu pembangkit semangat warga sekitar untuk mengembangkan potensi wisataDesa Sumberwuluh. Hasil proses pemberdayaan petani kopi yang didahului dengan penelitian di Desa Sumberwuluhberlangsung selama satu bulan, dalam tiga tahapan, yaitu tahap layying the foundation, research planning, gathering dan acting on finding. Langkah itu dilakukan untuk menelusuri lebih dalam kehidupan dan potensi yang ada Desa Sumberwuluh. Strategi dalam pengembangan kapasitas petani kopi hingga menghasilkan beberapa olahan dari kopi robusta, antara lain kopi original, kopi jahe dan kue muffin coffee. Hasil dari pemberdayaan petani kopi ini, bertujuan untuk menambah nilai ekonomi potensi desa dan estetika pengemasan produk kopisebagai olahan kopi dengan sentuhan modern sebagai buah tangan di Wisata Gunung Wayang yang menjadi produk dari Desa Sumberwuluh. Bentuk-bentuk pemberdayaan petani kopi melibatkan partisipasi masyarakat mulai dari perencanaan, pelaksanaan hingga evaluasi. Proses awal pemberdayaan membutuhkan bantuan stakeholder lokal dalam membangun komitmen masyarakat dalam mengembangkan wisata Gunung Wayang Desa Sumberwuluh. Kata Kunci: Pemberdayaan Petani Kopi; Gunung Wayang; Pengembangan Desa Wisat

Regulation of expression of the rat orthologue of mouse double minute 2 (MDM2) by H2O2-induced oxidative stress in neonatal rat cardiac myocytes.

The Mdm2 ubiquitin ligase is an important regulator of p53 abundance and p53-dependent apoptosis. Mdm2 expression is frequently regulated by a p53 Mdm2 autoregulatory loop whereby p53 stimulates Mdm2 expression and hence its own degradation. Although extensively studied in cell lines, relatively little is known about Mdm2 expression in heart where oxidative stress (exacerbated during ischemia-reperfusion) is an important pro-apoptotic stimulus. We demonstrate that Mdm2 transcript and protein expression are induced by oxidative stress (0.2 mm H(2)O(2)) in neonatal rat cardiac myocytes. In other cells, constitutive Mdm2 expression is regulated by the P1 promoter (5' to exon 1), with inducible expression regulated by the P2 promoter (in intron 1). In myocytes, H(2)O(2) increased Mdm2 expression from the P2 promoter, which contains two p53-response elements (REs), one AP-1 RE, and two Ets REs. H(2)O(2) did not detectably increase expression of p53 mRNA or protein but did increase expression of several AP-1 transcription factors. H(2)O(2) increased binding of AP-1 proteins (c-Jun, JunB, JunD, c-Fos, FosB, and Fra-1) to an Mdm2 AP-1 oligodeoxynucleotide probe, and chromatin immunoprecipitation assays showed it increased binding of c-Jun or JunB to the P2 AP-1 RE. Finally, antisense oligonucleotide-mediated reduction of H(2)O(2)-induced Mdm2 expression increased caspase 3 activation. Thus, increased Mdm2 expression is associated with transactivation at the P2 AP-1 RE (rather than the p53 or Ets REs), and Mdm2 induction potentially represents a cardioprotective response to oxidative stress

Urban–rural differences of gynaecological malignancies in Egypt (1999–2002)

Please cite this paper as: Dey S, Hablas A, Seifeldin I, Ismail K, Ramadan M, El-Hamzawy H, Wilson M, Banerjee M, Boffetta P, Harford J, Merajver S, Soliman A. Urban–rural differences of gynaecological malignancies in Egypt (1999–2002). BJOG 2010;117:348–355.In previous studies, we have shown a three to four times higher urban incidence of breast cancer and estrogen receptor-positive breast cancers in the Gharbiah Province of Egypt. We investigated the urban–rural incidence differences of gynaecologic malignancies (uterine, ovarian and cervical cancers) to explore if they show the same trend that we found for breast cancer.Cancer registry-based incidence comparison.Gharbiah population-based cancer registry (GPCR), Tanta, Egypt.All patients with uterine, ovarian and cervical cancer in GPCR from 1999 to 2002.We calculated uterine, ovarian and cervical cancer incidence from 1999 to 2002. For each of the three cancers, we calculated the overall and age-specific rates for the province as a whole, and by urban–rural status, as well as for the eight districts of the province.Incidence of all three cancer sites was higher in urban than in rural areas. Uterine cancer showed the highest urban–rural incidence rate ratio (IRR = 6.07, 95% CI = 4.17, 8.85). Uterine cancer also showed the highest urban incidence in the oldest age group (70+ age category, IRR = 14.39, 95% CI = 4.24, 48.87) and in developed districts (Tanta, IRR = 4.14, 95% CI = 0.41, 42.04). Incidence rates by groups of cancer sites showed an increasing gradient of urban incidence for cancers related to hormonal aetiology, mainly of the breast and uterus (IRR = 4.96, 95% CI = 2.86, 8.61).The higher urban incidence of uterine cancer, coupled with our previous findings of higher incidence of breast cancer and estrogen receptor positive breast cancer in urban areas in this region, may be suggestive of possible higher exposure to environmental estrogenic compounds, such as xenoestrogens, in urban areas.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/78726/1/j.1471-0528.2009.02447.x.pd

How to reach a few percent level in determining the Lense-Thirring effect?

In this paper we discuss and compare a node-only LAGEOS-LAGEOS II combination and a node-only LAGEOS-LAGEOS II-Ajisai-Jason1 combination for the determination of the Lense-Thirring effect. The new combined EIGEN-CG01C Earth gravity model has been adopted. The second combination cancels the first three even zonal harmonics along with their secular variations but introduces the non-gravitational perturbations of Jason1. The first combination is less sensitive to the non-conservative forces but is sensitive to the secular variations of the uncancelled even zonal harmonics of low degree J4 and J6 whose impact grows linearly in time.Comment: Latex2e, 22 pag. 1 table, 2 figures, 45 references. Changes in the Abstract, Introduction and Conclusions. Discussion on the non-gravitational perturbations on Ajisai and on the impact of the secular rates of the even zonal harmonics added. EIGEN-CG01C CHAMP+GRACE+terrestrial gravimetry/altimetry Earth gravity model used. Reference adde

Perception of general and oral health in White and African American adults: assessing the effect of neighborhood socioeconomic conditions 1

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/74693/1/j.1600-0528.2004.00177.x.pd

Opposite poles: A comparison between two Spanish regions in health-related quality of life, with implications for health policy

<p>Abstract</p> <p>Background</p> <p>Although health is one of the main determinants of the welfare of societies, few studies have evaluated health related quality of life in representative samples of the population of a region or a country. Our aim is to describe the health-related quality of life of the inhabitants of two quite different Spanish regions (Canary Islands and Catalonia) and to compare the prevalence of health problems between age-sex groups.</p> <p>Methods</p> <p>We use data obtained from the 2006 Health Survey of Catalonia and the 2004 Canary Islands Health Survey. With an ordinal composite variable measuring HRQOL we identify the association of characteristics of individuals with self-reported quality of life and test for differences between the regions.</p> <p>Results</p> <p>The prevalence of problems in the five EQ-5 D dimensions increases with age and is generally higher for women than for men. The dimension with the highest prevalence of problems is "anxiety/depression", and there is noteworthy the extent of discomfort and pain among Canary Island women. Education, especially among the elderly, has an important effect on health-related quality of life.</p> <p>Conclusions</p> <p>There are substantial structural and compositional differences between the two regions. Regional context is a significant factor, independent of the compositional differences, and the effects of context are manifest above all in women. The findings show the importance of disease prevention and the need for improving the educational level of the population in order to reduce health inequalities.</p

Coralline algae (Rhodophyta) in a changing world: integrating ecological, physiological, and geochemical responses to global change

Coralline algae are globally distributed benthic primary producers that secrete calcium carbonate skeletons. In the context of ocean acidification, they have received much recent attention due to the potential vulnerability of their high-Mg calcite skeletons and their many important ecological roles. Herein, we summarize what is known about coralline algal ecology and physiology, providing context to understand their responses to global climate change. We review the impacts of these changes, including ocean acidification, rising temperatures, and pollution, on coralline algal growth and calcification. We also assess the ongoing use of coralline algae as marine climate proxies via calibration of skeletal morphology and geochemistry to environmental conditions. Finally, we indicate critical gaps in our understanding of coralline algal calcification and physiology and highlight key areas for future research. These include analytical areas that recently have become more accessible, such as resolving phylogenetic relationships at all taxonomic ranks, elucidating the genes regulating algal photosynthesis and calcification, and calibrating skeletal geochemical metrics, as well as research directions that are broadly applicable to global change ecology, such as the importance of community-scale and long-term experiments in stress response