Earliest direct evidence of plant processing in prehistoric Saharan pottery

The invention of thermally resistant ceramic cooking vessels around 15,000 years ago was a major advance in human diet and nutrition(1-3), opening up new food groups and preparation techniques. Previous investigations of lipid biomarkers contained in food residues have routinely demonstrated the importance of prehistoric cooking pots for the processing of animal products across the world(4). Remarkably, however, direct evidence for plant processing in prehistoric pottery has not been forthcoming, despite the potential to cook otherwise unpalatable or even toxic plants(2,5). In North Africa, archaeobotanical evidence of charred and desiccated plant organs denotes that Early Holocene hunter-gatherers routinely exploited a wide range of plant resources(6). Here, we reveal the earliest direct evidence for plant processing in pottery globally, from the sites of Takarkori and Uan Afuda in the Libyan Sahara, dated to 8200-6400 bc. Characteristic carbon number distributions and \u3b4(13)C values for plant wax-derived n-alkanes and alkanoic acids indicate sustained and systematic processing of C3/C4 grasses and aquatic plants, gathered from the savannahs and lakes in the Early to Middle Holocene green Sahara

Climate change in healthcare: exploring the potential role of inhaler prescribing

Climate change has been described as the biggest global health threat of the 21st century. As a result, governments around the world are committing to legislative change in order to reduce greenhouse gas emissions (GHGEs). The healthcare sector makes a significant contribution to GHGEs and in line with national legislation in the UK, the NHS has recently committed to achieving net zero emissions by 2050. The management of asthma and COPD largely depends on the prescribing of medications that are delivered through inhalers. In the UK, the use of pressurized metered dose inhalers (pMDIs), which rely on hydrofluorocarbon (HFC) propellants accounts for 3.5% of the NHS’s total carbon footprint. In contrast, dry powder inhalers (DPIs) have a much lower carbon footprint due to the absence of a HFC propellant. Here we review evidence of the impact of inhaler choices across four domains: environmental impact, clinical effectiveness, cost effectiveness and patient preferences. We find that as well as a lower global‐warming potential, DPIs have additional benefits over pMDIs in other domains and should be considered first line where clinically appropriate

Reconciling atmospheric and oceanic views of the transient climate response to emissions

The Transient Climate Response to Emissions (TCRE), the ratio of surface warming and cumulative carbon emissions, is controlled by a product of thermal and carbon contributions. The carbon contribution involves the airborne fraction and the ratio of ocean saturated and atmospheric carbon inventories, with this ratio controlled by ocean carbonate chemistry. The evolution of the carbon contribution to the TCRE is illustrated in a hierarchy of models: a box model of the atmosphere‐ocean and an Earth system model, both integrated for 1,000 years, and a suite of Earth system models integrated for 140 years. For all models, there is the same generic carbonate chemistry response: An acidifying ocean during emissions leads to a decrease in the ratio of the ocean saturated and atmospheric carbon inventories and the carbon contribution to the TCRE. Hence, ocean carbonate chemistry is important in controlling the magnitude of the TCRE and its evolution in time

Food-web structure in relation to environmental gradients and predator-prey ratios in tank-bromeliad ecosystems

Little is known of how linkage patterns between species change along environmental gradients. The small, spatially discrete food webs inhabiting tank-bromeliads provide an excellent opportunity to analyse patterns of community diversity and food-web topology (connectance, linkage density, nestedness) in relation to key environmental variables (habitat size, detrital resource, incident radiation) and predators: prey ratios. We sampled 365 bromeliads in a wide range of understorey environments in French Guiana and used gut contents of invertebrates to draw the corresponding 365 connectance webs. At the bromeliad scale, habitat size (water volume) determined the number of species that constitute food-web nodes, the proportion of predators, and food-web topology. The number of species as well as the proportion of predators within bromeliads declined from open to forested habitats, where the volume of water collected by bromeliads was generally lower because of rainfall interception by the canopy. A core group of microorganisms and generalist detritivores remained relatively constant across environments. This suggests that (i) a highly-connected core ensures food-web stability and key ecosystem functions across environments, and (ii) larger deviations in food-web structures can be expected following disturbance if detritivores share traits that determine responses to environmental changes. While linkage density and nestedness were lower in bromeliads in the forest than in open areas, experiments are needed to confirm a trend for lower food-web stability in the understorey of primary forests

A census of metals and baryons in stars in the local Universe

We combine stellar metallicity and stellar mass estimates for a large sample of galaxies drawn from the SDSS DR2 spanning wide ranges in physical properties, in order to derive an inventory of the total mass of metals and baryons locked up in stars today. Physical parameter estimates are derived from galaxy spectra with high S/N (>20). Coadded spectra of galaxies with similar velocity dispersions, absolute r-band magnitudes and 4000\AA-break values are used for those regions of parameter space where individual spectra have lower S/N. We estimate the total density of metals and of baryons in stars and, from these two quantities, we obtain a mass- and volume-averaged stellar metallicity of =1.04+-0.14 Z_sun, i.e. consistent with solar. We also study how metals are distributed in galaxies according to their mass, morphology and age, and we then compare these distributions with the corresponding distributions of stellar mass. We find that the bulk of metals locked up in stars in the local Universe reside in massive, bulge-dominated galaxies, with red colours and high 4000\AA-break values corresponding to old stellar populations. Bulge-dominated and disc-dominated galaxies contribute similar amounts to the total stellar mass density, but have different fractional contributions to the mass density of metals in stars, in agreement with the mass-metallicity relation. Bulge-dominated galaxies contain roughly 40% of the total amount of metals in stars, while disc-dominated galaxies less than 25%. Finally, at a given galaxy stellar mass, we define two characteristic ages as the median of the distributions of mass and metals as a function of age. These characteristic ages decrease progressively from high-mass to low-mass galaxies, consistent with the high formation epochs of stars in massive galaxies.Comment: replaced with accepted version, minor changes, references adde

Telehealth-delivered cognitive rehabilitation for people with cognitive impairment as part of the post-COVID syndrome: protocol for a randomised controlled trial as part of the CICERO (Cognitive Impairment in Long COVID: Phenotyping and Rehabilitation) study

BackgroundBetween 25 and 75% of people with persistent post-acute sequelae of SARS-CoV-2 infection (PASC) experience cognitive difficulties, compromising functional ability, quality of life, and activities of daily living, including work. Despite this significant morbidity, there is a paucity of interventions for this disorder that have undergone evaluation within a formal trial setting. Therefore, we have developed a cognitive rehabilitation programme, specifically designed to address the cognitive symptoms of PASC, notably impaired attention and processing speed, while also accounting for other PASC symptoms (fatigue, post-exertional malaise) that may aggravate the cognitive impairment. This study protocol outlines a randomised controlled trial (RCT) designed to evaluate the effectiveness of this programme compared to standard clinical care.MethodsThis is a multi-centre, parallel-group, individually randomised controlled trial, comparing standard clinical care with and without cognitive rehabilitation. We will recruit 120 non-hospitalised adults (aged 30–60 years) from three NHS sites in England with a history of COVID-19 infection and cognitive impairment persisting more than 3 months after the acute infection. Participants will be randomised (1:1) to the intervention or control groups, with the latter represented as a provision of standard clinical care without cognitive rehabilitation. The cognitive rehabilitation programme consists of ten 1-hour sessions, delivered weekly. Outcomes will be collected at baseline, 3, and 6 months, with participant-defined goal-attainment scores, relating to functional goals, at 3 months as the primary outcome measure. Secondary outcomes will be cognitive function, measures of quality of life, social functioning, mental health, fatigue, sleep, post-exertional malaise, and social and health care service use. We will also evaluate the health-economic benefits of cognitive rehabilitation in this population.DiscussionCognitive impairment in PASC is a major cause of functional disability with no effective treatment. Accordingly, we will undertake an RCT of cognitive rehabilitation, the protocol of which is published here. If this trial is successful in delivering improvements in trial outcomes, it will address a major unmet need relating to this emergent disorder, with a significant impact on affected individuals and the wider health economy.Trial registrationClinicalTrials.gov NCT05731570. Registered on February 16, 202

Inhibition of Ubc13-mediated ubiquitination by GPS2 regulates multiple stages of B cell development

Non-proteolytic ubiquitin signaling mediated by Lys63 ubiquitin chains plays a critical role in multiple pathways that are key to the development and activation of immune cells. Our previous work indicates that GPS2 (G-protein Pathway Suppressor 2) is a multifunctional protein regulating TNF signaling and lipid metabolism in the adipose tissue through modulation of Lys63 ubiquitination events. However, the full extent of GPS2-mediated regulation of ubiquitination and the underlying molecular mechanisms are unknown. Here, we report that GPS2 is required for restricting the activation of TLR and BCR signaling pathways and the AKT/FOXO1 pathway in immune cells based on direct inhibition of Ubc13 enzymatic activity. Relevance of this regulatory strategy is confirmed in vivo by B cell-targeted deletion of GPS2, resulting in developmental defects at multiple stages of B cell differentiation. Together, these findings reveal that GPS2 genomic and non-genomic functions are critical for the development and cellular homeostasis of B cells