Silencing of the human microsomal glucose-6-phosphate translocase induces glioma cell death: Potential new anticancer target for curcumin

AbstractG6P translocase (G6PT) is thought to play a crucial role in transducing intracellular signaling events in brain tumor-derived cancer cells. In this report, we investigated the contribution of G6PT to the control of U-87 brain tumor-derived glioma cell survival using small interfering RNA (siRNA)-mediated suppression of G6PT. Three siRNA constructs were generated and found to suppress up to 91% G6PT gene expression. Flow cytometry analysis of propidium iodide/annexin-V-stained cells indicated that silencing the G6PT gene induced necrosis and late apoptosis. The anticancer agent curcumin, also inhibited G6PT gene expression by more than 90% and triggered U-87 glioma cells death. Overexpression of recombinant G6PT rescued the cells from curcumin-induced cell death. Targeting G6PT expression may provide a new mechanistic rationale for the action of chemopreventive drugs and lead to the development of new anti-cancer strategies

The immunopathology of ANCA-associated vasculitis.

The small-vessel vasculitides are a group of disorders characterised by variable patterns of small blood vessel inflammation producing a markedly heterogeneous clinical phenotype. While any vessel in any organ may be involved, distinct but often overlapping sets of clinical features have allowed the description of three subtypes associated with the presence of circulating anti-neutrophil cytoplasmic antibodies (ANCA), namely granulomatosis with polyangiitis (GPA, formerly known as Wegener's Granulomatosis), microscopic polyangiitis (MPA) and eosinophilic granulomatosis with polyangiitis (eGPA, formerly known as Churg-Strauss syndrome). Together, these conditions are called the ANCA-associated vasculitidies (AAV). Both formal nomenclature and classification criteria for the syndromes have changed repeatedly since their description over 100 years ago and may conceivably do so again following recent reports showing distinct genetic associations of patients with detectable ANCA of distinct specificities. ANCA are not only useful in classifying the syndromes but substantial evidence implicates them in driving disease pathogenesis although the mechanism by which they develop and tolerance is broken remains controversial. Advances in our understanding of the pathogenesis of the syndromes have been accompanied by some progress in treatment, although much remains to be done to improve the chronic morbidity associated with the immunosuppression required for disease control

Observation of Top Quark Production in Proton-Nucleus Collisions

Peer reviewe

Effects of some botanical extracts on the control of major insect pests of okra in the Sudan Savannah Agro-Ecological Zone of Nigeria

Two location trials were conducted in 2018 dry season at the Teaching and Research Farm, Faculty of Agriculture, University of Maiduguri (Unimaid) and demonstration site of the Mohamet Lawan College of Agriculture (MOLCA) Maiduguri to assess the insecticidal potentials of three botanicals and a control in the management of Attractomorpha acutipennis, Amarasca biguttula biguttula, Aphis gossypii and Bemisia tabaci infestation on okra. These treatments were laid out in randomized complete block design (RCBD) replicated three times. Data collected include the number of damaged and undamaged leaves and the number of insect pests. Using Analysis of variance (ANOVA), results showed that the extracts significantly (p < 0.05) reduced the population of the insects and the number of damaged leaves when compared with control in both locations. Plots treated with Balanite aegytiaca extract recorded the lowers population of Attractomorpha acutipennis, A. biguttula biguttula, A. gossypii and B. tabaci. Similarly, Balanite aegytiaca extract recorded the lower leaves damaged than plots treated with Eucalyptus camaldulensis extract in both locations. Among all the treatments evaluated, Plots treated with Balanite aegytiaca extract significantly recorded the least number of A. gossypii and A. biguttula biguttula in both locations. However, plots treated with Balanite aegytiaca extract significantly gave the least number of B. tabaci at MOLCA while plots treated with Eucalyptus camaldulensis extract gave the least number of the insect at UNIMAID. There was no significant difference in the performance of Balanite aegytiaca and Eucalyptus camaldulensis extract. Balanite aegytiaca extract also recorded the least number of damaged leaves in both locations

Reconstruction of plasma edge density profile from Lithium beam data using statistical analysis

[No abstract available