Shining new light on mammalian diving physiology using wearable near-infrared spectroscopy

Investigation of marine mammal dive-by-dive blood distribution and oxygenation has been limited by a lack of non-invasive technology for use in freely diving animals. Here, we developed a non-invasive near-infrared spectroscopy (NIRS) device to measure relative changes in blood volume and haemoglobin oxygenation continuously in the blubber and brain of voluntarily diving harbour seals. Our results show that seals routinely exhibit preparatory peripheral vasoconstriction accompanied by increased cerebral blood volume approximately 15 s before submersion. These anticipatory adjustments confirm that blood redistribution in seals is under some degree of cognitive control that precedes the mammalian dive response. Seals also routinely increase cerebral oxygenation at a consistent time during each dive, despite a lack of access to ambient air. We suggest that this frequent and reproducible reoxygenation pattern, without access to ambient air, is underpinned by previously unrecognised changes in cerebral drainage. The ability to track blood volume and oxygenation in different tissues using NIRS will facilitate a more accurate understanding of physiological plasticity in diving animals in an increasingly disturbed and exploited environment

Interviewing suspects: examining the association between skills, questioning, evidence disclosure, and interview outcomes

The interviewing of suspects is an important element in the investigation of crime. However, studies concerning actual performance of investigators when undertaking such interviews remain sparse. Nevertheless, in England and Wales, since the introduction of a prescribed framework over 20 years ago, field studies have generally shown an improvement in interviewing performance, notwithstanding ongoing concerns largely relating to the more demanding aspects (such as building/maintaining rapport, intermittent summarising and the logical development of topics). Using a sample of 70 real-life interviews, the present study examined questioning and various evidence disclosure strategies (which have also been found demanding), examining their relationships between interview skills and interview outcomes. It was found that when evidence was disclosed gradually (but revealed later), interviews were generally both more skilled and involved the gaining of comprehensive accounts, whereas when evidence was disclosed either early or very late, interviews were found to be both less skilled and less likely to involve this outcome. These findings contribute towards an increased research base for the prescribed framework

Increased metabolic rate of hauled-out harbor seals (Phoca vitulina) during the molt

Harbor seals (Phoca vitulina) live in cold temperate or polar seas and molt annually, renewing their fur over a period of approximately four weeks. Epidermal processes at this time require a warm skin and therefore to avoid an excessive energy cost at sea during the molt, harbour seals and many other pinnipeds increase the proportion of time hauled out on land. We predicted that metabolic rate during haulout would be greater during the molt to sustain an elevated skin temperature in order to optimize skin and hair growth. To examine this, we measured post-haulout oxygen consumption (V̇O2) in captive harbor seals during molt and post-molt periods. We recorded greater V̇O2 of seals while molting than when the molt was complete. Post-haulout V̇O2 increased faster and reached a greater maximum at 40 minutes during the molt. Thereafter, V̇O2 decreased but still remained greater suggesting that while metabolic rate was relatively high throughout haulouts, it was most pronounced in the first 40 minutes. Air temperature, estimated heat increment of feeding (eHIF) and mass also explained 15.5% of V̇O2 variation over 180 minutes post-haulout, suggesting that the environment, feeding state and body size influenced the metabolic rate of individual animals. These results show that moulting seals have greater metabolic rates when hauled out, especially during the early stages of the haulout period. As a consequence, human disturbance that changes the haulout behaviour of molting seals will increase their energy costs and potentially extend the duration of the molt

One way or another? Criminal investigators' beliefs regarding the disclosure of evidence in interviews with suspects in England and Wales

The research base concerning interviews with suspects remains to be comprehensively developed. For example, the extant literature provides differing views regarding how best to undertake the important interview task of disclosing evidence. In the current study, using a self-report questionnaire, 224 investigators based in England and Wales were asked as to their own preferred methods. Most respondents advocated a gradual method of disclosing evidence, stating that this approach would better reveal inconsistencies and obtain a complete version of events (similar to the reasoning of those who preferred disclosing evidence later). Those who advocated revealing evidence early stated this approach would more likely elicit confessions. Several respondents would not commit to one single method, arguing that their chosen strategy was contextually dependent. The study’s findings suggest that it remains arguable as to whether there is one best approach to evidence disclosure and/or whether particular circumstances should influence interviewing strategies

BrAPI-an application programming interface for plant breeding applications

Motivation: Modern genomic breeding methods rely heavily on very large amounts of phenotyping and genotyping data, presenting new challenges in effective data management and integration. Recently, the size and complexity of datasets have increased significantly, with the result that data are often stored on multiple systems. As analyses of interest increasingly require aggregation of datasets from diverse sources, data exchange between disparate systems becomes a challenge. Results: To facilitate interoperability among breeding applications, we present the public plant Breeding Application Programming Interface (BrAPI). BrAPI is a standardized web service API specification. The development of BrAPI is a collaborative, community-based initiative involving a growing global community of over a hundred participants representing several dozen institutions and companies. Development of such a standard is recognized as critical to a number of important large breeding system initiatives as a foundational technology. The focus of the first version of the API is on providing services for connecting systems and retrieving basic breeding data including germplasm, study, observation, and marker data. A number of BrAPI-enabled applications, termed BrAPPs, have been written, that take advantage of the emerging support of BrAPI by many databases

Source Handler telephone interactions with covert human intelligence sources: An exploration of question types and intelligence yield

Law Enforcement Agencies gather intelligence in order to prevent criminal activity and pursue criminals. In the context of human intelligence collection, intelligence elicitation relies heavily upon the deployment of appropriate evidence‐based interviewing techniques (a topic rarely covered in the extant research literature). The present research gained unprecedented access to audio recorded telephone interactions (N = 105) between Source Handlers and Covert Human Intelligence Sources (CHIS) from England and Wales. The research explored the mean use of various question types per interaction and across all questions asked in the sample, as well as comparing the intelligence yield for appropriate and inappropriate questions. Source Handlers were found to utilise vastly more appropriate questions than inappropriate questions, though they rarely used open‐ended questions. Across the total interactions, appropriate questions (by far) were associated with the gathering of much of the total intelligence yield. Implications for practise are discussed

Global patient outcomes after elective surgery: prospective cohort study in 27 low-, middle- and high-income countries.

BACKGROUND: As global initiatives increase patient access to surgical treatments, there remains a need to understand the adverse effects of surgery and define appropriate levels of perioperative care. METHODS: We designed a prospective international 7-day cohort study of outcomes following elective adult inpatient surgery in 27 countries. The primary outcome was in-hospital complications. Secondary outcomes were death following a complication (failure to rescue) and death in hospital. Process measures were admission to critical care immediately after surgery or to treat a complication and duration of hospital stay. A single definition of critical care was used for all countries. RESULTS: A total of 474 hospitals in 19 high-, 7 middle- and 1 low-income country were included in the primary analysis. Data included 44 814 patients with a median hospital stay of 4 (range 2-7) days. A total of 7508 patients (16.8%) developed one or more postoperative complication and 207 died (0.5%). The overall mortality among patients who developed complications was 2.8%. Mortality following complications ranged from 2.4% for pulmonary embolism to 43.9% for cardiac arrest. A total of 4360 (9.7%) patients were admitted to a critical care unit as routine immediately after surgery, of whom 2198 (50.4%) developed a complication, with 105 (2.4%) deaths. A total of 1233 patients (16.4%) were admitted to a critical care unit to treat complications, with 119 (9.7%) deaths. Despite lower baseline risk, outcomes were similar in low- and middle-income compared with high-income countries. CONCLUSIONS: Poor patient outcomes are common after inpatient surgery. Global initiatives to increase access to surgical treatments should also address the need for safe perioperative care. STUDY REGISTRATION: ISRCTN5181700

Big data and understanding change in the context of planning transport systems

This paper considers the implications of so-called ‘big data’ for the analysis, modelling and planning of transport systems. The primary conceptual focus is on the needs of the practical context of medium-term planning and decision-making, from which perspective the paper seeks to achieve three goals: (i) to try to identify what is truly ‘special’ about big data; (ii) to provoke debate on the future relationship between transport planning and big data; and (iii) to try to identify promising themes for research and application. Differences in the information that can be derived from the data compared to more traditional surveys are discussed, and the respects in which they may impact on the role of models in supporting transport planning and decision-making are identified. It is argued that, over time, changes to the nature of data may lead to significant differences in both modelling approaches and in the expectations placed upon them. Furthermore, it is suggested that the potential widespread availability of data to commercial actors and travellers will affect the performance of the transport systems themselves, which might be expected to have knock-on effects for planning functions. We conclude by proposing a series of research challenges that we believe need to be addressed and warn against adaptations based on minimising change from the status quo

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead