79 research outputs found
Contribution of siderophore systems to growth and urinary tract colonization of asymptomatic bacteriuria Escherichia coli
The molecular mechanisms that define asymptomatic bacteriuria (ABU) E. coli colonization of the human urinary tract remain to be properly elucidated. Here we utilize ABU E. coli strain 83972 as a model to dissect the contribution of siderophores to iron acquisition, growth, fitness and colonization of the urinary tract. We show that E. coli 83972 produces enterobactin, salmochelin, aerobactin and yersiniabactin, and examine the role of these systems using mutants defective in siderophore biosynthesis and uptake. Enterobactin and aerobactin contributed most to total siderophore activity and growth in defined iron-deficient media. No siderophores were detected in an 83972 quadruple mutant deficient in all four siderophore biosynthesis pathways; this mutant did not grow in defined iron-deficient media but grew in iron-limited pooled human urine due to iron uptake via the FecA ferric citrate receptor. In a mixed 1:1 growth assay with 83972 there was no fitness disadvantage of the 83972 quadruple biosynthetic mutant, demonstrating its capacity to act as a âcheaterâ and utilize siderophores produced by the wild-type strain for iron uptake. An 83972 enterobactin/salmochelin double receptor mutant was outcompeted by 83972 in human urine and the mouse urinary tract, indicating a role for catecholate receptors in urinary tract colonization
All-sky convolution for polarimetry experiments
We discuss all-sky convolution of the instrument beam with the sky signal in
polarimetry experiments, such as the Planck mission which will map the
temperature anisotropy and polarization of the cosmic microwave background
(CMB). To account properly for stray light (from e.g. the galaxy, sun, and
planets) in the far side-lobes of such an experiment, it is necessary to
perform the beam convolution over the full sky. We discuss this process in
multipole space for an arbitrary beam response, fully including the effects of
beam asymmetry and cross-polarization. The form of the convolution in multipole
space is such that the Wandelt-Gorski fast technique for all-sky convolution of
scalar signals (e.g. temperature) can be applied with little modification. We
further show that for the special case of a pure co-polarized, axisymmetric
beam the effect of the convolution can be described by spin-weighted window
functions. In the limits of a small angle beam and large Legendre multipoles,
the spin-weight 2 window function for the linear polarization reduces to the
usual scalar window function used in previous analyses of beam effects in CMB
polarimetry experiments. While we focus on the example of polarimetry
experiments in the context of CMB studies, we emphasise that the formalism we
develop is applicable to anisotropic filtering of arbitrary tensor fields on
the sphere.Comment: 8 pages, 1 figure; Minor changes to match version accepted by Phys.
Rev.
Harmonic analysis of cosmic microwave background data I: ring reductions and point-source catalogue
We present a harmonic model for the data analysis of an all-sky cosmic
microwave background survey, such as Planck, where the survey is obtained
through ring-scans of the sky. In this model, resampling and pixelisation of
the data are avoided. The spherical transforms of the sky at each frequency, in
total intensity and polarization, as well as the bright-point-source catalogue,
are derived directly from the data reduced onto the rings. Formal errors and
the most significant correlation coefficients for the spherical transforms of
the frequency maps are preserved. A clean and transparent path from the
original samplings in the time domain to the final scientific products is thus
obtained. The data analysis is largely based on Fourier analysis of rings; the
positional stability of the instrument's spin axis during these scans is a
requirement for the data model and is investigated here for the Planck
satellite. Brighter point sources are recognised and extracted as part of the
ring reductions and, on the basis of accumulated data, used to build a
catalogue. The analysis of the rings is performed iteratively, involving a
range of geometric and detector response calibrations. The reconstructed
spherical transforms of the sky form the input to the subsequent analysis
stages. Although the methods in this paper were developed with the data
processing for Planck in mind, many aspects should have wider application, such
as in the construction of real-space pixelised maps. (Abridged)Comment: 20 pages, 9 figures. To appear in MNRA
Recent trends and developments in pyrolysis-gas chromatography: review
Pyrolysis-gas chromatography (Py-GC) has become well established as a simple, quick and reliable analytical technique for a range of applications including the analysis of polymeric materials. Recent developments in Py-GC technology and instrumentation include laser pyrolysis and non-discriminating pyrolysis. Progress has also been made in the detection of low level polymer additives with the use of novel Py-GC devices. Furthermore, it has been predicted that future advances in separation technology such as the use of comprehensive two-dimensional gas chromatography will further enhance the analytical scope of Py-GC
The redshift evolution of the distribution of star formation among dark matter halos as seen in the infrared
Recent studies revealed a strong correlation between the star formation rate (SFR) and stellar mass of star-forming galaxies, the so-called star-forming main sequence. An empirical modeling approach (2-SFM) which distinguishes between the main sequence and rarer starburst galaxies is capable of reproducing most statistical properties of infrared galaxies. In this paper, we extend this approach by establishing a connection between stellar mass and halo mass with the technique of abundance matching. Based on a few, simple assumptions and a physically motivated formalism, our model successfully predicts the (cross-)power spectra of the cosmic infrared background (CIB), the cross-correlation between CIB and cosmic microwave background (CMB) lensing, and the correlation functions of bright, resolved infrared galaxies measured by Herschel, Planck, ACT and SPT. We use this model to infer the redshift distribution these observables, as well as the level of correlation between CIB-anisotropies at different wavelengths. We also predict that more than 90% of cosmic star formation activity occurs in halos with masses between 10^11.5 and 10^13.5 Msun. Taking into account subsequent mass growth of halos, this implies that the majority of stars were initially (at z>3) formed in the progenitors of clusters, then in groups at 0.5<z<3 and finally in Milky-Way-like halos at z<0.5. At all redshifts, the dominant contribution to the star formation rate density stems from halos of mass ~10^12 Msun, in which the instantaneous star formation efficiency is maximal (~70%). The strong redshift-evolution of SFR in the galaxies that dominate the CIB is thus plausibly driven by increased accretion from the cosmic web onto halos of this characteristic mass scale
Mass and Hot Baryons in Massive Galaxy Clusters from Subaru Weak Lensing and AMiBA SZE Observations
We present a multiwavelength analysis of a sample of four hot (T_X>8keV)
X-ray galaxy clusters (A1689, A2261, A2142, and A2390) using joint AMiBA
Sunyaev-Zel'dovich effect (SZE) and Subaru weak lensing observations, combined
with published X-ray temperatures, to examine the distribution of mass and the
intracluster medium (ICM) in massive cluster environments. Our observations
show that A2261 is very similar to A1689 in terms of lensing properties. Many
tangential arcs are visible around A2261, with an effective Einstein radius
\sim 40 arcsec (at z \sim 1.5), which when combined with our weak lensing
measurements implies a mass profile well fitted by an NFW model with a high
concentration c_{vir} \sim 10, similar to A1689 and to other massive clusters.
The cluster A2142 shows complex mass substructure, and displays a shallower
profile (c_{vir} \sim 5), consistent with detailed X-ray observations which
imply recent interaction. The AMiBA map of A2142 exhibits an SZE feature
associated with mass substructure lying ahead of the sharp north-west edge of
the X-ray core suggesting a pressure increase in the ICM. For A2390 we obtain
highly elliptical mass and ICM distributions at all radii, consistent with
other X-ray and strong lensing work. Our cluster gas fraction measurements,
free from the hydrostatic equilibrium assumption, are overall in good agreement
with published X-ray and SZE observations, with the sample-averaged gas
fraction of = 0.133 \pm 0.027, for our sample = (1.2 \pm
0.1) \times 10^{15} M_{sun} h^{-1}. When compared to the cosmic baryon fraction
f_b = \Omega_b/\Omega_m constrained by the WMAP 5-year data, this indicates
/f_b = 0.78 \pm 0.16, i.e., (22 \pm 16)% of the baryons are missing
from the hot phase of clusters.Comment: accepted for publication in ApJ; high resolution figures available at
http://www.asiaa.sinica.edu.tw/~keiichi/upfiles/AMiBA7/ms_highreso.pd
A meta-analysis of long-term effects of conservation agriculture on maize grain yield under rain-fed conditions
Conservation agriculture involves reduced tillage, permanent soil cover and crop rotations to enhance soil fertility and to supply food from a dwindling land resource. Recently, conservation agriculture has been promoted in Southern Africa, mainly for maize-based farming systems. However, maize yields under rain-fed conditions are often variable. There is therefore a need to identify factors that influence crop yield under conservation agriculture and rain-fed conditions. Here, we studied maize grain yield data from experiments lasting 5 years and more under rain-fed conditions. We assessed the effect of long-term tillage and residue retention on maize grain yield under contrasting soil textures, nitrogen input and climate. Yield variability was measured by stability analysis. Our results show an increase in maize yield over time with conservation agriculture practices that include rotation and high input use in low rainfall areas. But we observed no difference in system stability under those conditions. We observed a strong relationship between maize grain yield and annual rainfall. Our meta-analysis gave the following findings: (1) 92% of the data show that mulch cover in high rainfall areas leads to lower yields due to waterlogging; (2) 85% of data show that soil texture is important in the temporal development of conservation agriculture effects, improved yields are likely on well-drained soils; (3) 73% of the data show that conservation agriculture practices require high inputs especially N for improved yield; (4) 63% of data show that increased yields are obtained with rotation but calculations often do not include the variations in rainfall within and between seasons; (5) 56% of the data show that reduced tillage with no mulch cover leads to lower yields in semi-arid areas; and (6) when adequate fertiliser is available, rainfall is the most important determinant of yield in southern Africa. It is clear from our results that conservation agriculture needs to be targeted and adapted to specific biophysical conditions for improved impact
PRISM (Polarized Radiation Imaging and Spectroscopy Mission): A White Paper on the Ultimate Polarimetric Spectro-Imaging of the Microwave and Far-Infrared Sky
PRISM (Polarized Radiation Imaging and Spectroscopy Mission) was proposed to
ESA in response to the Call for White Papers for the definition of the L2 and
L3 Missions in the ESA Science Programme. PRISM would have two instruments: (1)
an imager with a 3.5m mirror (cooled to 4K for high performance in the
far-infrared---that is, in the Wien part of the CMB blackbody spectrum), and
(2) an Fourier Transform Spectrometer (FTS) somewhat like the COBE FIRAS
instrument but over three orders of magnitude more sensitive. Highlights of the
new science (beyond the obvious target of B-modes from gravity waves generated
during inflation) made possible by these two instruments working in tandem
include: (1) the ultimate galaxy cluster survey gathering 10e6 clusters
extending to large redshift and measuring their peculiar velocities and
temperatures (through the kSZ effect and relativistic corrections to the
classic y-distortion spectrum, respectively) (2) a detailed investigation into
the nature of the cosmic infrared background (CIB) consisting of at present
unresolved dusty high-z galaxies, where most of the star formation in the
universe took place, (3) searching for distortions from the perfect CMB
blackbody spectrum, which will probe a large number of otherwise inaccessible
effects (e.g., energy release through decaying dark matter, the primordial
power spectrum on very small scales where measurements today are impossible due
to erasure from Silk damping and contamination from non-linear cascading of
power from larger length scales). These are but a few of the highlights of the
new science that will be made possible with PRISM.Comment: 20 pages Late
The Atacama Cosmology Telescope: A Measurement of the DR6 CMB Lensing Power Spectrum and its Implications for Structure Growth
We present new measurements of cosmic microwave background (CMB) lensing over
sq. deg. of the sky. These lensing measurements are derived from the
Atacama Cosmology Telescope (ACT) Data Release 6 (DR6) CMB dataset, which
consists of five seasons of ACT CMB temperature and polarization observations.
We determine the amplitude of the CMB lensing power spectrum at
precision ( significance) using a novel pipeline that minimizes
sensitivity to foregrounds and to noise properties. To ensure our results are
robust, we analyze an extensive set of null tests, consistency tests, and
systematic error estimates and employ a blinded analysis framework. The
baseline spectrum is well fit by a lensing amplitude of
relative to the Planck 2018 CMB power spectra
best-fit CDM model and relative to
the best-fit model. From our lensing power
spectrum measurement, we derive constraints on the parameter combination
of
from ACT DR6 CMB lensing alone and
when combining ACT DR6 and Planck NPIPE
CMB lensing power spectra. These results are in excellent agreement with
CDM model constraints from Planck or
CMB power spectrum measurements. Our lensing measurements from redshifts
-- are thus fully consistent with CDM structure growth
predictions based on CMB anisotropies probing primarily . We find no
evidence for a suppression of the amplitude of cosmic structure at low
redshiftsComment: 45+21 pages, 50 figures. Prepared for submission to ApJ. Also see
companion papers Madhavacheril et al and MacCrann et a
The Atacama Cosmology Telescope: DR6 Gravitational Lensing Map and Cosmological Parameters
We present cosmological constraints from a gravitational lensing mass map
covering 9400 sq. deg. reconstructed from CMB measurements made by the Atacama
Cosmology Telescope (ACT) from 2017 to 2021. In combination with BAO
measurements (from SDSS and 6dF), we obtain the amplitude of matter
fluctuations at 1.8% precision,
and the Hubble
constant at
1.6% precision. A joint constraint with CMB lensing measured by the Planck
satellite yields even more precise values: ,
and . These measurements agree
well with CDM-model extrapolations from the CMB anisotropies measured
by Planck. To compare these constraints to those from the KiDS, DES, and HSC
galaxy surveys, we revisit those data sets with a uniform set of assumptions,
and find from all three surveys are lower than that from ACT+Planck
lensing by varying levels ranging from 1.7-2.1. These results motivate
further measurements and comparison, not just between the CMB anisotropies and
galaxy lensing, but also between CMB lensing probing on
mostly-linear scales and galaxy lensing at on smaller scales. We
combine our CMB lensing measurements with CMB anisotropies to constrain
extensions of CDM, limiting the sum of the neutrino masses to eV (95% c.l.), for example. Our results provide independent
confirmation that the universe is spatially flat, conforms with general
relativity, and is described remarkably well by the CDM model, while
paving a promising path for neutrino physics with gravitational lensing from
upcoming ground-based CMB surveys.Comment: 30 pages, 16 figures, prepared for submission to ApJ. Cosmological
likelihood data is here:
https://lambda.gsfc.nasa.gov/product/act/actadv_prod_table.html ; likelihood
software is here: https://github.com/ACTCollaboration/act_dr6_lenslike . Also
see companion papers Qu et al and MacCrann et al. Mass maps will be released
when papers are publishe
- âŠ