O AVANÇO DA OCUPAÇÃO ANTRÓPICA EM ÁREA DE RISCO NA SERRA DO TEPEQUÉM - RR

A serra do Tepequém, no Norte do estado de Roraima, tem passado por intensas modificações em sua paisagem ocasionada tanto por ação natural como antrópica. A ocupação na região tem contribuído para retirada da vegetação e abertura de estradas, o que intensifica a vulnerabilidade do solo e os impactos ambientais e antrópicos. Na serra, as construções ocupam áreas próximas a encostas, rede de drenagem e feições erosivas lineares que apresentam dinâmica constante em decorrência da ação climática

Histone variant MacroH2A1 is downregulated in prostate cancer and influences malignant cell phenotype

Background: Prostate cancer (PCa), a major cause of cancer-related morbidity and mortality worldwide and mostly asymptomatic at earliest stages, is characterized by disruption of genetic and epigenetic balance. A better understanding of how those mechanisms orchestrate disease might improve diagnostic and prognostic tools, allowing for improvements in treatment efficacy. Replacement of canonical histones, an epigenetic mechanism, is highly conserved among species and altered expression of histones variants (e.g., MacroH2A1) has been associated with tumorigenesis. H2AFY gene encodes two isoforms of H2A histone variant MacroH2A1: MacroH2A1.1 and MacroH2A1.2. Specifically, MacroH2A1.1 isoform inhibits cell proliferation and promotes cellular differentiation. Because the contribution of this histone variant to carcinogenesis has been reported in several cancer types, but not for PCa, we aimed to investigate the contribution of MacroH2A1 for prostate carcinogenesis. Methods: MacroH2A1, MacroH2A1.1 and MacroH2A1.2 isoforms and the corresponding splicing regulators transcript levels were evaluated by RT-qPCR, in a tissue cohort composed by PCa, prostatic intraepithelial neoplasia (PIN) and normal prostate cases. Knockdown for MacroH2A1 and MacroH2A1.1 was performed through lentiviral transduction in DU145 cells, and MacroH2A1.1 overexpression was achieved in LNCaP cells by plasmid transfection, followed by functional assays. Biological and/or experimental replicates were performed when necessary, and specific statistical tests were applied to perform data analysis. Results: MacroH2A1.1 transcript levels were downregulated in PIN and primary PCa compared to normal prostate tissues. The same was found for QKI, a MacroH2A1.1's splicing regulator. Moreover, lower MacroH2A1.1 and QKI expression levels associated with less differentiated tumors (Gleason score ≥ 7). Interestingly, MacroH2A1.1, but more impressively DDX17 (AUC = 0.93; p < 0.0001) and QKI (AUC = 0.94; p < 0.0001), accurately discriminated cancerous from noncancerous prostate tissues. Furthermore, in PCa cell lines, total MacroH2A1 knockdown augmented malignant features, whereas MacroH2A1.1 overexpression impressively attenuated the malignant phenotype. Conclusions: Overall, our data, derived from primary PCa tissues and cell lines, anticipate a tumor suppressive role for MacroH2A1, particularly for the MacroH2A1.1 isoform, in prostate carcinogenesis

High-resolution monitoring of diffuse (sheet or interrill) erosion using structure-from-motion

Sheet erosion is common on agricultural lands, and understanding the dynamics of the erosive process as well as the quantification of soil loss is important for both soil scientists and managers. However, measuring rates of soil loss from sheet erosion has proved difficult due to requiring the detection of relatively small surface changes over extended areas. Consequently, such measurements have relied on the use of erosion plots, which have limited spatial coverage and have high operating costs. For measuring the larger erosion rates characteristic of rill and gully erosion, structure-from-motion (SfM) photogrammetry has been demonstrated to be a valuable tool. Here, we demonstrate the first direct validation of UAV-SfM measurements of sheet erosion using sediment collection data collected from erosion plots. Three erosion plots (12 m × 4 m) located at Lavras, Brazil, with bare soil exposed to natural rainfall from which event sediment and runoff was monitored, were mapped during two hydrological years (2016 and 2017), using a UAV equipped with a RGB camera. DEMs of difference (DoD) were calculated to detect spatial changes in the soil surface topography over time and to quantify the volumes of sediments lost or gained. Precision maps were generated to enable precision estimates for both DEMs to be propagated into the DoD as spatially variable vertical uncertainties. The point clouds generated from SfM gave mean errors of ~2.4 mm horizontally (xy) and ~1.9 mm vertically (z) on control and independent check points, and the level of detection (LoD) along the plots ranged from 1.4 mm to 7.4 mm. The soil loss values obtained by SfM were significantly (p < 0.001) correlated (r 2 = 95.55%) with those derived from the sediment collection. These results open up the possibility to use SfM for erosion studies where channelized erosion is not the principal mechanism, offering a cost-effective method for gaining new insights into sheet, and interrill, erosion processes

Search for the standard model Higgs boson decaying into two photons in pp collisions at sqrt(s)=7 TeV

A search for a Higgs boson decaying into two photons is described. The analysis is performed using a dataset recorded by the CMS experiment at the LHC from pp collisions at a centre-of-mass energy of 7 TeV, which corresponds to an integrated luminosity of 4.8 inverse femtobarns. Limits are set on the cross section of the standard model Higgs boson decaying to two photons. The expected exclusion limit at 95% confidence level is between 1.4 and 2.4 times the standard model cross section in the mass range between 110 and 150 GeV. The analysis of the data excludes, at 95% confidence level, the standard model Higgs boson decaying into two photons in the mass range 128 to 132 GeV. The largest excess of events above the expected standard model background is observed for a Higgs boson mass hypothesis of 124 GeV with a local significance of 3.1 sigma. The global significance of observing an excess with a local significance greater than 3.1 sigma anywhere in the search range 110-150 GeV is estimated to be 1.8 sigma. More data are required to ascertain the origin of this excess.Comment: Submitted to Physics Letters

Measurement of isolated photon production in pp and PbPb collisions at sqrt(sNN) = 2.76 TeV

Isolated photon production is measured in proton-proton and lead-lead collisions at nucleon-nucleon centre-of-mass energies of 2.76 TeV in the pseudorapidity range |eta|<1.44 and transverse energies ET between 20 and 80 GeV with the CMS detector at the LHC. The measured ET spectra are found to be in good agreement with next-to-leading-order perturbative QCD predictions. The ratio of PbPb to pp isolated photon ET-differential yields, scaled by the number of incoherent nucleon-nucleon collisions, is consistent with unity for all PbPb reaction centralities.Comment: Submitted to Physics Letters