Suplementação materna de juçara (Euterpe Edulis Mart): na modulação dos marcadores inflamatórios e da microbiota da prole aos 21 dias de vida

Background. Maternal intake of TFAs in the perinatal period triggers a proinflammatory state and adiposity in offspring. However, phenol compounds contained in fruits are promising modulators of inflammation. Aim. This study investigated the effect of Jussara supplementation in the maternal diet on the development, microbiota and proinflammatory state of the colon in offspring exposed to perinatal TFAs. Methods. On the first day of pregnancy rats were divided into four groups: control diet (C), control diet with 0.5% Jussara supplementation (CJ), diet enriched with hydrogenated vegetable fat, rich in TFAs (T), or T diet supplemented with 0.5% Jussara (TJ) during pregnancy and lactation. Dams’ diets were maintained during pregnancy and lactation. After birth, litter sizes were adjusted to eight pups that remained with the mother. After 21 days, the offspring were decapitated. Trunk blood was collected and centrifuged. The RET, liver, colon and the fecal content were collected. Colon, liver and RET were used for quantification of mRNA relative levels by real-time PCR or perform Western Blotting. The genomic bacterial DNA in gut microbiota was performed by real-time PCR. The carcasses were eviscerated and used to determine lipid and protein content. Results. Maternal intake of TFAs resulted in increased IL-6 in RET and colon, TNF-�� e mRNA TLR-4 no cólon, MyD88 in the liver and a reduction in IL-10/TNF-α ratio and Bifidobacterium spp. in the offspring’s colon. However, Jussara supplementation restored the fecal content of Lactobacillus spp. e Bifidobacterium spp. (TJ vs T), increased colonic ZO-1 mRNA expression (TJ vs T), and decreased MyD88, p-NFkB p65 subunit (TJ e CJ vs T) and TNF-��R1 (TJ e CJ vs C) in the liver. These effects reduced IL-6 and TNF-α in the liver and colon, mRNA expression of IL-6R (CJ e TJ vs T; CJ vs C), TNF-��R1 (CJ e TJ vs T) and TLR-4 (CJ e TJ vs T) in the colon, increased of IL-10 in the RET and IL-10/TNF-α ratio in the liver (TJ vs T). Additionally, the jussara supplementation groups showed similar growth, less weight gain (CJ vs C, T and TJ) and better body composition, reflected by reduced lipid/protein ratio (CJ and TJ vs T) and higher carcass protein (TJ vs T). The jussara supplementation in maternal diet reduced lipids profile and glucose in offspring. Conclusion. The 0.5% Jussara supplementation prevented the adverse effects of TFAs, improved lipid profiles, glucose levels, body composition, restored gut microbiota and reduced low-grade inflammation in 21-day-old offspring via down-regulation of the NFkB signaling pathway. These findings suggests better paracellular barrier and could contribute to reduced inflammation and chronic disease development in later life.Introdução. Estudos prévios do nosso grupo de trabalho sugerem que a ingestão materna de ácidos graxos trans (AGT) promove aumento da adiposidade e estado próinflamatório na prole. Entretanto, compostos fenólicos presentes em frutos, a exemplo da Juçara, têm sido reportados como promissores na modulação da inflamação e estresse oxidativo em humanos e animais adultos. Objetivo. Investigar o efeito da suplementação materna com a Juçara (Euterpe edulis Mart.) sobre o desenvolvimento, microbiota e estado pró-inflamatório da prole com 21 dias de vida de ratas tratadas com ácidos graxos trans durante a gestação e lactação. Métodos. No primeiro dia de gestação, as ratas foram randomizadas em quatro grupos: dieta controle (C), dieta C suplementada com 0,5% de polpa da Juçara liofilizada (CJ), dieta enriquecida com gordura vegetal hidrogenada, rica em AGT (T) ou dieta T suplementada com 0,5% de polpa da Juçara liofilizada (TJ). As dietas foram mantidas durante todo o período de gestação e lactação. As proles fizeram parte dos grupos de suas respectivas mães e aos 21 dias de vida foram submetidos à eutanásia. O sangue, tecido adiposo retroperitoneal (RET), fígado, cólon e fezes do cólon foram coletados. Foram realizadas dosagens bioquímicas no soro. O cólon, fígado e RET foram utilizados para determinação dos níveis de RNAm por PCR em tempo real ou para realização de Western Blotting. O DNA genômico da microbiota colônica foi determinado por PCR em tempo real. As carcaças foram evisceradas e utilizadas para determinação da gordura e proteína. Resultados. A ingestão materna de AGT resultou em aumento de IL-6 no RET e cólon, TNF-�� e mRNA TLR-4 no cólon, MyDD88 no fígado e redução da relação IL-10/TNF-α e Bifidobacterium spp. no cólon. No entanto, a suplementação com a juçara na dieta materna restaurou Lactobacillus spp. e Bifidobacterium spp. (TJ vs T), aumentou a expressão gênica de ZO-1 no cólon (TJ vs T), reduziu a expressão protéica de MyD88, da subunidade p-NFκBp65 (TJ e CJ vs T) e TNF-��R1 (TJ e CJ vs C) no fígado, o que levou a redução das citocinas pró-inflamatórias IL-6 e TNF-α no fígado e cólon, da expressão gênica dos receptores IL-6R (CJ e TJ vs T; CJ vs C), TNF-��R1 (CJ e TJ vs T) e TLR-4 (CJ e TJ vs T) no cólon, aumento de IL-10 no RET e da relação IL-10/TNF-α no fígado (TJ vs T). Além disso, os grupos que receberam a suplementação com a Juçara na dieta materna apresentaram crescimento semelhante aos demais grupos, com menor ganho de peso (CJ vs C, T e TJ) e melhor composição corporal, refletida pelo menor peso relativo da gordura (CJ e TJ vs C), da relação gordura/proteína (CJ e TJ vs T) e maior proteína da carcaça (TJ vs T). A suplementação com a Juçara na dieta materna também reduziu o colesterol total (CJ e TJ vs T), triacilglicerol (CJ e TJ vs T e C) e glicose (CJ e TJ vs T; Grupo TJ vs C). Conclusão. A ingestão materna de juçara melhora a composição corporal, perfil lipídico, glicose sérica, restaura a microbiota e reduz os marcadores inflamatórios na prole de 21 dias de vida, envolvendo a redução da ativação da via de sinalização do NFκB. Estes resultados sugerem melhor função da barreira intestinal da prole e podem contribuir para o controle da inflamação e prevenção de doenças crônicas na vida adulta.Dados abertos - Sucupira - Teses e dissertações (2013 a 2016

Jussara (Euterpe edulis Mart.) Supplementation during Pregnancy and Lactation Modulates the Gene and Protein Expression of Inflammation Biomarkers Induced by trans-Fatty Acids in the Colon of Offspring

Maternal intake of trans-fatty acids (TFAs) in the perinatal period triggers a proinflammatory state in offspring. Anthocyanins contained in fruit are promising modulators of inflammation. This study investigated the effect of Jussara supplementation in the maternal diet on the proinflammatory state of the colon in offspring exposed to perinatal TFAs. On the first day of pregnancy rats were divided into four groups: control diet (C), control diet with 0.5% Jussara supplementation (CJ), diet enriched with hydrogenated vegetable fat, rich in TFAs (T), or T diet supplemented with 0.5% Jussara (TJ) during pregnancy and lactation. We showed that Jussara supplementation inmaternal diet (CJ and TJ groups) reduced carcass lipid/protein ratios, serumlipids, glucose, IL-6, TNF-alpha, gene expression of IL-6R, TNF-alpha R (P < 0.05), TLR-4 (P < 0.01), and increase Lactobacillus spp. (P < 0.05) in the colon of offspring compared to the T group. the IL-10 (P = 0.035) and IL-10/TNF-alpha ratio (P < 0.01) was higher in the CJ group than in the T group. the 0.5% Jussara supplementation reverses the adverse effects of perinatal TFAs, improving lipid profiles, glucose levels, body composition, and gut microbiota and reducing low-grade inflammation in the colon of 21-day-old offspring, and could contribute to reducing chronic disease development.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de São Paulo, Programa Posgrad Interdisciplinar Ciencias Saude, BR-11015020 Santos, SP, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Fisiol Nutr, São Paulo, BrazilUniversidade Federal de São Paulo, Inst Saude & Sociedade, Dept Biociencias, BR-11015020 Santos, SP, BrazilUniversidade Federal de São Paulo, Programa Posgrad Interdisciplinar Ciencias Saude, BR-11015020 Santos, SP, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Fisiol Nutr, São Paulo, BrazilUniversidade Federal de São Paulo, Inst Saude & Sociedade, Dept Biociencias, BR-11015020 Santos, SP, BrazilWeb of Scienc

MENOPAUSA E SUAS CONSEQUÊNCIAS PSICOLÓGICAS.

Introduction: Menopause, a natural stage in women's lives, marks the end of the reproductive period and is often accompanied by hormonal and physical changes. Furthermore, menopause can have significant implications for women's mental health, influencing their emotional and psychological states. Objective: This study aims to investigate the psychological consequences of menopause in women, exploring the emotional and behavioral impacts associated with this transition phase. Methodology: This is an integrative literature review, with an approach that allows the synthesis of studies with different methodologies, including qualitative, quantitative and mixed studies. Searches were carried out in electronic databases, such as PubMed, PsycINFO and Scopus, using keywords related to menopause and mental health. Studies published in the last 10 years were selected. Results: The results highlighted a variety of psychological consequences related to menopause. Many participants reported symptoms of anxiety, depression and mood swings. In addition, self-esteem and body image were affected in some cases. Quality of life was also perceived as compromised due to these emotional challenges. Final Considerations: The study highlights the importance of understanding the psychological implications of menopause and recognizing that this phase can be accompanied by significant emotional difficulties for some women. The multidimensional approach, which considers both the biological and psychological aspects of menopause, is essential to provide adequate support to women in this transition.Introdução: A menopausa, um estágio natural na vida de mulheres, marca o fim do período reprodutivo e é frequentemente acompanhada por mudanças hormonais e físicas. Além disso, a menopausa pode ter implicações significativas para a saúde mental das mulheres, influenciando seus estados emocionais e psicológicos. Objetivo: Este estudo tem como objetivo investigar as consequências psicológicas da menopausa em mulheres, explorando os impactos emocionais e comportamentais associados a essa fase de transição. Metodologia: Trata-se de uma revisão integrativa da literatura, sendo uma abordagem que permite a síntese de estudos de diferentes metodologias, incluindo estudos qualitativos, quantitativos e mistos. Foram realizadas buscas em bases de dados eletrônicas, como PubMed, PsycINFO e Scopus, utilizando palavras-chave relacionadas à menopausa e saúde mental. Foram selecionados estudos publicados nos últimos 10 anos. Resultados: Os resultados destacaram uma variedade de consequências psicológicas relacionadas à menopausa. Muitas participantes relataram sintomas de ansiedade, depressão e oscilações de humor. Além disso, a autoestima e a imagem corporal foram afetadas em alguns casos. A qualidade de vida também foi percebida como comprometida devido a esses desafios emocionais. Considerações Finais: O estudo evidencia a importância de compreender as implicações psicológicas da menopausa e reconhecer que essa fase pode ser acompanhada por dificuldades emocionais significativas para algumas mulheres. A abordagem multidimensional, que considera tanto os aspectos biológicos quanto os psicológicos da menopausa, é essencial para fornecer um suporte adequado às mulheres nessa transição

ARIA digital anamorphosis : Digital transformation of health and care in airway diseases from research to practice

Digital anamorphosis is used to define a distorted image of health and care that may be viewed correctly using digital tools and strategies. MASK digital anamorphosis represents the process used by MASK to develop the digital transformation of health and care in rhinitis. It strengthens the ARIA change management strategy in the prevention and management of airway disease. The MASK strategy is based on validated digital tools. Using the MASK digital tool and the CARAT online enhanced clinical framework, solutions for practical steps of digital enhancement of care are proposed.Peer reviewe

ARIA digital anamorphosis: Digital transformation of health and care in airway diseases from research to practice

Digital anamorphosis is used to define a distorted image of health and care that may be viewed correctly using digital tools and strategies. MASK digital anamorphosis represents the process used by MASK to develop the digital transformation of health and care in rhinitis. It strengthens the ARIA change management strategy in the prevention and management of airway disease. The MASK strategy is based on validated digital tools. Using the MASK digital tool and the CARAT online enhanced clinical framework, solutions for practical steps of digital enhancement of care are proposed

Anthocyanins as inflammatory modulators and the role of the gut microbiota

The health benefits of consuming fruits that are rich in polyphenols, especially anthocyanins, have been the focus of recent in vitro and in vivo investigations. Thus, greater attention is being directed to the reduction of the inflammatory process associated with the intestinal microbiota and the mechanism underlying these effects because the microbiota has been closely associated with the metabolism of these compounds in the gastrointestinal tract. Further interest lies in the ability of these metabolites to modulate the growth of specific intestinal bacteria. Thus, this review examines studies involving the action of the anthocyanins that are present in many fruits and their effect in the modulating the inflammatory process associated with the interaction between the host and the gut microbiota. The findings of both in vitro and in vivo studies suggest a potential antiinflammatory effect of these compounds, which seem to inhibit activation of the signaling pathway mediated by the transcription factor NF kappa B. This effect is associated with modulation of a beneficial gut microbiota, particularly an increase in Bifidobacterium strains. (C) 2015 Elsevier Inc. All rights reserved.Fundacao de Amparo a Pesquisa do Estado de Sao PauloUniv Fed Sao Paulo, Inst Saude & Soc, Dept Biociencias, Santos, SP, BrazilUniv Fed Sao Paulo, Inst Saude & Soc, Dept Biociencias, Santos, SP, BrazilFAPESP: 2013/25101-4Web of Scienc

Jussara (Euterpe edulis Mart.) supplementation during pregnancy and lactation modulates UCP-1 and inflammation biomarkers induced by trans-fatty acids in the brown adipose tissue of offspring

BACKGROUND &amp; AIMS: The brown adipose tissue (BAT) regulates energy expenditure via thermogenesis by uncoupling protein 1 (UPC-1). We investigated the effect of the maternal diet enriched with trans-fatty acids (TFAs) in combination or not with jussara fruit supplementation on the 21 day old offspring. Specifically, we looked at the proinflammatory state and the expression of UCP-1 in the offsprings' BAT. METHODS: We divided dams into four groups during pregnancy and lactation: control diet (C), C diet supplemented with 0.5% of jussara fruit rich in anthocyanins and monounsaturated fatty acids (CJ), a diet enriched with hydrogenated vegetable oil (T), rich in TFAs, or T diet supplemented with 0.5% of jussara fruit (TJ). RESULTS: We found that TFAs reduced growth and increased weight, total cholesterol, TNF-α and TNFRI in BAT of pups. Conversely, maternal supplementation with jussara preserved lean mass, decreased weight gain, carcass lipid, blood glucose and triacylglycerol in the offsprings, it also increased IL-10 levels in the BAT. Surprisingly, jussara supplementation in the TFAs group increased UCP-1 expression in the BAT. CONCLUSIONS: While TFAs are detrimental for the offsprings' health, jussara fruit improved body composition, and the metabolic and inflammatory parameters. Furthermore, the combination of both treatments (TFAs and jussara supplementation) seen in the TJ group raised UCP-1 expression. We believe that the bioactive compounds of jussara contributed to these effects. Our results showed that maintaining the caloric amount in the maternal diet but modifying its lipid composition with a low fat quality as well as adding a natural fruit could epigenetically program the BAT of 21 day old offspring