Isolation and characterisation of mesenchymal stem cells from rat bone marrow and the endosteal niche: A comparative study

Within bone, mesenchymal stromal cells (MSCs) exist within the bone marrow stroma (BM-MSC) and the endosteal niche, as cells lining compact bone (CB-MSCs). This study isolated and characterised heterogeneous MSC populations from each niche and subsequently investigated the effects of extensive cell expansion, analysing population doublings (PDs)/cellular senescence, colony-forming efficiencies (CFEs), MSC cell marker expression, and osteogenic/adipogenic differentiation. CB-MSCs and BM-MSCs demonstrated similar morphologies and PDs, reaching 100 PDs. Both populations exhibited consistent telomere lengths (12–17 kb), minimal senescence, and positive telomerase expression. CB-MSCs (PD15) had significantly lower CFEs than PD50. CB-MSCs and BM-MSCs both expressed MSC (CD73/CD90/CD105); embryonic (Nanog) and osteogenic markers (Runx2, osteocalcin) but no hematopoietic markers (CD45). CB-MSCs (PD15) strongly expressed Oct4 and p16INK4A. At early PDs, CB-MSCs possessed a strong osteogenic potency and low potency for adipogenesis, whilst BM-MSCs possessed greater overall bipotentiality for osteogenesis and adipogenesis. At PD50, CB-MSCs demonstrated reduced potency for both osteogenesis and adipogenesis, compared to BM-MSCs at equivalent PDs. This study demonstrates similarities in proliferative and mesenchymal cell characteristics between CB-MSCs and BM-MSCs, but contrasting multipotentiality. Such findings support further comparisons of human CB-MSCs and BM-MSCs, facilitating selection of optimal MSC populations for regenerative medicine purposes

The Bolocam Galactic Plane Survey: Survey Description and Data Reduction

We present the Bolocam Galactic Plane Survey (BGPS), a 1.1 mm continuum survey at 33" effective resolution of 170 square degrees of the Galactic Plane visible from the northern hemisphere. The survey is contiguous over the range -10.5 < l < 90.5, |b| < 0.5 and encompasses 133 square degrees, including some extended regions |b| < 1.5. In addition to the contiguous region, four targeted regions in the outer Galaxy were observed: IC1396, a region towards the Perseus Arm, W3/4/5, and Gem OB1. The BGPS has detected approximately 8400 clumps over the entire area to a limiting non-uniform 1-sigma noise level in the range 11 to 53 mJy/beam in the inner Galaxy. The BGPS source catalog is presented in a companion paper (Rosolowsky et al. 2010). This paper details the survey observations and data reduction methods for the images. We discuss in detail the determination of astrometric and flux density calibration uncertainties and compare our results to the literature. Data processing algorithms that separate astronomical signals from time-variable atmospheric fluctuations in the data time-stream are presented. These algorithms reproduce the structure of the astronomical sky over a limited range of angular scales and produce artifacts in the vicinity of bright sources. Based on simulations, we find that extended emission on scales larger than about 5.9' is nearly completely attenuated (> 90%) and the linear scale at which the attenuation reaches 50% is 3.8'. Comparison with other millimeter-wave data sets implies a possible systematic offset in flux calibration, for which no cause has been discovered. This presentation serves as a companion and guide to the public data release through NASA's Infrared Processing and Analysis Center (IPAC) Infrared Science Archive (IRSA). New data releases will be provided through IPAC IRSA with any future improvements in the reduction.Comment: Accepted for publication in Astrophysical Journal Supplemen

Development of mathematical pathways for VET students to articulate to related higher education courses: a focus on engineering

Australia needs more qualified professionals in the Science, Technology, Engineering, and Mathematics (STEM) areas. The national focus on widening participation in higher education (HE) includes strengthening pathways from vocational education and training (VET). VET students often lack the mathematics skills necessary to articulate successfully to their chosen university degrees. Current approaches such as bridging and foundation mathematics programs are not tailored or sufficiently contextualised for VET articulants. This project is developing a mathematics pathway designed to improve the readiness of VET engineering diploma graduates for higher education study in engineering degree programs. Arrangements are flexible so that students can complete these pathways either as part of their engineering diploma as a VET student or as part of preparatory study at the diploma level at university. Many VET students are granted credit when entering a HE course in engineering and can transfer directly to second year units which may assume a level of mathematical knowledge by the university. However, in the VET Diploma of Engineering Technical (MEM50212), there is only one core unit in mathematics (MEM30012A) equivalent to year 9 level and there are two mathematics electives, MEM23004A and MEM23007A, which are part of the advanced diploma and often not taught by many TAFE providers due to student demand and staff capabilities. The lack of required mathematics often leaves the student with a large gap in the required knowledge for success in HE. The project has been underway for over a year and significant progress has been made in developing the pathway for engineering. To date, the mathematical knowledge outcomes from the VET courses have been mapped to the requirements of the HE courses at the University of Tasmania, Flinders University and James Cook University. Gaps in mathematical knowledge have been identified. A formal articulation agreement has been established through TasTAFE and the University of Tasmania where current VET students will be able to enroll in the university foundation mathematics units and receive credit towards their VET diploma in engineering. In addition to the foundation units, the students need to do an online component. This consists of a few compulsory topics which are not covered in the foundation units with supporting examples, practice problems, practical application and self-assessed quizzes for each mathematics topic covered in the foundation units, contextualised to engineering. VET students are applied learners and therefore often struggle with the transition to HE. The online component of the pathway is designed to support the student by providing the context to the mathematics they are learning. Another advantage of the pathway is that it exposes the VET students to HE units and the university environment while satisfying the university mathematics entry requirements

Simulations of the Milky Way's central molecular zone -- I. Gas dynamics

We use hydrodynamical simulations to study the Milky Way's central molecular zone (CMZ). The simulations include a non-equilibrium chemical network, the gas self-gravity, star formation and supernova feedback. We resolve the structure of the interstellar medium at sub-parsec resolution while also capturing the interaction between the CMZ and the bar-driven large-scale flow out to R\sim 5\kpc. Our main findings are as follows: (1) The distinction between inner ($R\lesssim120$~pc) and outer ($120\lesssim R\lesssim450$~pc) CMZ that is sometimes proposed in the literature is unnecessary. Instead, the CMZ is best described as single structure, namely a star-forming ring with outer radius $R\simeq 200$~pc which includes the 1.3$^\circ$ complex and which is directly interacting with the dust lanes that mediate the bar-driven inflow. (2) This accretion can induce a significant tilt of the CMZ out of the plane. A tilted CMZ might provide an alternative explanation to the $\infty$-shaped structure identified in Herschel data by Molinari et al. 2011. (3) The bar in our simulation efficiently drives an inflow from the Galactic disc ($R\simeq 3$~kpc) down to the CMZ ($R\simeq200$~pc) of the order of $1\rm\,M_\odot\,yr^{-1}$, consistent with observational determinations. (4) Supernova feedback can drive an inflow from the CMZ inwards towards the circumnuclear disc of the order of $\sim0.03\,\rm M_\odot\,yr^{-1}$. (5) We give a new interpretation for the 3D placement of the 20 and 50 km s$^{-1}$ clouds, according to which they are close ($R\lesssim30$~pc) to the Galactic centre, but are also connected to the larger-scale streams at $R\gtrsim100$~pc.Comment: Accepted for publication in MNRAS. Movies of the simulations can be found at: https://www.youtube.com/channel/UCwnzfO-xLxzRDz9XsexfPo

The Bolocam Galactic Plane Survey: II. Catalog of The Image Data

We present a catalog of 8358 sources extracted from images produced by the Bolocam Galactic Plane Survey (BGPS). The BGPS is a survey of the millimeter dust continuum emission from the northern Galactic plane. The catalog sources are extracted using a custom algorithm, Bolocat, which was designed specifically to identify and characterize objects in the large-area maps generated from the Bolocam instrument. The catalog products are designed to facilitate follow-up observations of these relatively unstudied objects. The catalog is 98% complete from 0.4 Jy to 60 Jy over all object sizes for which the survey is sensitive ( \u3c 3\u27.5). We find that the sources extracted can best be described as molecular clumps-large dense regions in molecular clouds linked to cluster formation. We find that the flux density distribution of sources follows a power law with dN/dS alpha S(-2.4+/-0.1) and that the mean Galactic latitude for sources is significantly below the midplane: \u3c b \u3e = (-0 degrees.095 +/- 0 degrees.001)

The Bolocam Galactic Plane Survey IV: 1.1 and 0.35 mm Dust Continuum Emission in the Galactic Center Region

The Bolocam Galactic Plane Survey (BGPS) data for a six square degree region of the Galactic plane containing the Galactic center is analyzed and compared to infrared and radio continuum data. The BGPS 1.1 mm emission consists of clumps interconnected by a network of fainter filaments surrounding cavities, a few of which are filled with diffuse near-IR emission indicating the presence of warm dust or with radio continuum characteristic of HII regions or supernova remnants. New 350 {\mu}m images of the environments of the two brightest regions, Sgr A and B, are presented. Sgr B2 is the brightest mm-emitting clump in the Central Molecular Zone and may be forming the closest analog to a super star cluster in the Galaxy. The Central Molecular Zone (CMZ) contains the highest concentration of mm and sub-mm emitting dense clumps in the Galaxy. Most 1.1 mm features at positive longitudes are seen in silhouette against the 3.6 to 24 {\mu}m background observed by the Spitzer Space Telescope. However, only a few clumps at negative longitudes are seen in absorption, confirming the hypothesis that positive longitude clumps in the CMZ tend to be on the near-side of the Galactic center, consistent with the suspected orientation of the central bar in our Galaxy. Some 1.1 mm cloud surfaces are seen in emission at 8 {\mu}m, presumably due to polycyclic aromatic hydrocarbons (PAHs). A ~0.2\degree (~30 pc) diameter cavity and infrared bubble between l \approx 0.0\degree and 0.2\degree surrounds the Arches and Quintuplet clusters and Sgr A. The bubble contains several clumpy dust filaments that point toward Sgr A\ast; its potential role in their formation is explored. [abstract truncated]Comment: 76 pages, 22 figures, published in ApJ: http://iopscience.iop.org/0004-637X/721/1/137

Efficient replication bypass of size-expanded DNA base pairs in bacterial cells

Author Manuscript 2012 September 06Supersize me! Size-expanded DNA bases (xDNA) are able to encode natural DNA sequences in replication. In vitro experiments with a DNA polymerase show nucleotide incorporation opposite the xDNA bases with correct pairing. In vivo experiments using E. coli show that two xDNA bases (xA and xC, see picture) encode the correct replication partners.National Institutes of Health (U.S.) (CA80024)National Institutes of Health (U.S.) (GM63587)Stanford Graduate Fellowshi

Food systems sustainability : an examination of different viewpoints on food system change

Global food insecurity levels remain stubbornly high. One of the surest ways to grasp the scale and consequence of global inequality is through a food systems lens. In a predominantly urban world, urban food systems present a useful lens to engage a wide variety of urban (and global) challenges—so called ‘wicked problems.’ This paper describes a collaborative research project between four urban food system research units, two European and two African. The project purpose was to seek out solutions to what lay between, across and within the different approaches applied in the understanding of each city’s food system challenges. Contextual differences and immediate (perceived) needs resulted in very different views on the nature of the challenge and the solutions required. Value positions of individuals and their disciplinary “enclaves” presented further boundaries. The paper argues that finding consensus provides false solutions. Rather the identification of novel approaches to such wicked problems is contingent of these differences being brought to the fore, being part of the conversation, as devices through which common positions can be discovered, where spaces are created for the realisation of new perspectives, but also, where difference is celebrated as opposed to censored

Enhanced hydrogen production from thermochemical processes

To alleviate the pressing problem of greenhouse gas emissions, the development and deployment of sustainable energy technologies is necessary. One potentially viable approach for replacing fossil fuels is the development of a H2 economy. Not only can H2 be used to produce heat and electricity, it is also utilised in ammonia synthesis and hydrocracking. H2 is traditionally generated from thermochemical processes such as steam reforming of hydrocarbons and the water-gas-shift (WGS) reaction. However, these processes suffer from low H2 yields owing to their reversible nature. Removing H2 with membranes and/or extracting CO2 with solid sorbents in situ can overcome these issues by shifting the component equilibrium towards enhanced H2 production via Le Chatelier's principle. This can potentially result in reduced energy consumption, smaller reactor sizes and, therefore, lower capital costs. In light of this, a significant amount of work has been conducted over the past few decades to refine these processes through the development of novel materials and complex models. Here, we critically review the most recent developments in these studies, identify possible research gaps, and offer recommendations for future research

Effects of antiplatelet therapy on stroke risk by brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases: subgroup analyses of the RESTART randomised, open-label trial

Background Findings from the RESTART trial suggest that starting antiplatelet therapy might reduce the risk of recurrent symptomatic intracerebral haemorrhage compared with avoiding antiplatelet therapy. Brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases (such as cerebral microbleeds) are associated with greater risks of recurrent intracerebral haemorrhage. We did subgroup analyses of the RESTART trial to explore whether these brain imaging features modify the effects of antiplatelet therapy