Democratic Middle Ground in Nepal: A Perspective from the North American Nepali Diaspora

The call of our time is to safeguard the accomplishments of the 1990 Peoples Movement, to restore sovereignty to the people, and to work towards the middle ground to resolve the nation\u27s core problems. History teaches us that recognizing, adopting and adhering to the middle path takes much vision and courage. The natural instinct is to stick to one\u27s own interpretation of the world (usually based on narrow self-interest) and to shun ideas and individuals that require a moderation of one\u27s views. However, success in politics and statecraft, more so than in any other area of human affairs, is hinged to the middle ground in a way that ultimately requires friend and foe to migrate sufficiently towards each other so that the peoples\u27 business can move forward and flourish. We urge all political forces in Nepal to recognize that great achievements in the affairs of nations come about when leaders practice the art of compromise. There is no dishonor for Nepal\u27s monarch and political leaders if they follow the path of the likes of Gandhi, Nehru and Mandela.\u2

The genetic architecture of the human cerebral cortex

The cerebral cortex underlies our complex cognitive capabilities, yet little is known about the specific genetic loci that influence human cortical structure. To identify genetic variants that affect cortical structure, we conducted a genome-wide association meta-analysis of brain magnetic resonance imaging data from 51,665 individuals. We analyzed the surface area and average thickness of the whole cortex and 34 regions with known functional specializations. We identified 199 significant loci and found significant enrichment for loci influencing total surface area within regulatory elements that are active during prenatal cortical development, supporting the radial unit hypothesis. Loci that affect regional surface area cluster near genes in Wnt signaling pathways, which influence progenitor expansion and areal identity. Variation in cortical structure is genetically correlated with cognitive function, Parkinson's disease, insomnia, depression, neuroticism, and attention deficit hyperactivity disorder

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Genomic and phylogenetic analysis of a multidrug-resistant Burkholderia contaminans strain isolated from a patient with ocular infection

ABSTRACT: Objectives: The genus Burkholderia comprises rod-shaped, non-spore-forming, obligately aerobic Gram-negative bacteria that is found across diverse ecological niches. Burkholderia contaminans, an emerging pathogen associated with cystic fibrosis, is frequently isolated from contaminated medical devices in hospital settings. The aim of this study was to understand the genomic characteristics, antimicrobial resistance profile and virulence determinants of B. contaminans strain SBC01 isolated from the eye of a patient hit by a cow's tail. Methods: A hybrid sequence of isolate SBC01 was generated using Illumina HiSeq and Oxford Nanopore Technology platforms. Unicycler was used to assemble the hybrid genomic sequence. The draft genome was annotated using the NCBI Prokaryotic Genome Annotation Pipeline. Antimicrobial susceptibility testing was performed by VITEK®2. Antimicrobial resistance and virulence genes were identified using validated bioinformatics tools. Results: The assembled genome size is 8 841 722 bp with a G+C content of 66.33% distributed in 19 contigs. Strain SBC01 was found to possess several antimicrobial resistance and efflux pump genes. The isolate was susceptible to tetracyclines, meropenem and ceftazidime. Many genes encoding potential virulence factors were identified. Conclusion: Burkholderia contaminans SBC01 belonging to sequence type 482 (ST482) is a multidrug-resistant strain containing diverse antimicrobial resistance genes, revealing the risks associated with infections by new Burkholderia spp. The large G+C-rich genome has a myriad of virulence factors, highlighting its pathogenic potential. Thus, while providing insights into the antimicrobial resistance and virulence potential of this uncommon species, the present analysis will aid in understanding the evolution and speciation in the Burkholderia genus

Data from: Female density-dependent chemical warfare underlies fitness effects of group sex ratio in flour beetles

In animals, skewed sex ratios can affect individual fitness either via sexual (e.g. intersexual conflict or intrasexual mate competition) or non-sexual interactions (e.g. sex-specific resource competition). Because most analyses of sex ratio focus on sexual interactions, the relative importance of sexual vs. non-sexual mechanisms remains unclear. We tested both mechanisms in the flour beetle Tribolium castaneum, where male-biased sex ratios increase female fitness relative to unbiased or female-biased groups. Although flour beetles show both sexual and non-sexual (resource) competition, we found that sexual interactions did not explain female fitness. Instead, female fecundity was dramatically reduced even after a brief exposure to flour conditioned by other females. Earlier studies suggested that secreted toxins might mediate density-dependent population growth in flour beetles. We identified ethyl- and methyl- benzoquinone (EBQ and MBQ; “quinones”), as components of adult stink glands that regulate female fecundity. In female-biased groups (i.e. at high female density), females upregulated quinones and suppressed each other’s reproduction. In male-biased groups, low female density and associated low quinone levels maximized fecundity. Thus, females appear to use quinones as weapons for female-specific, density-dependent interference competition. Our results underscore the importance of non-sexual interference competition that may often underlie the fitness consequences of skewed sex ratios

KhanEtal_AmNat_AllDataFiles

Raw data for all figures in the manuscrip

Democratic Middle Ground in Nepal: A Perspective from the North American Nepali Diaspora

The call of our time is to safeguard the accomplishments of the 1990 People’s Movement, to restore sovereignty to the people, and to work towards the middle ground to resolve the nation’s core problems. History teaches us that recognizing, adopting and adhering to the middle path takes much vision and courage. The natural instinct is to stick to one’s own interpretation of the world (usually based on narrow self-interest) and to shun ideas and individuals that require a moderation of one’s views. However, success in politics and statecraft, more so than in any other area of human affairs, is hinged to the middle ground in a way that ultimately requires friend and foe to migrate sufficiently towards each other so that the peoples’ business can move forward and flourish. We urge all political forces in Nepal to recognize that great achievements in the affairs of nations come about when leaders practice the art of compromise. There is no dishonor for Nepal’s monarch and political leaders if they follow the path of the likes of Gandhi, Nehru and Mandela.Nepal Study Center, University of New Mexic