17β-estradiol abrogates apoptosis inhibiting PKCδ, JNK and p66Shc activation in C2C12 cells

17β-Estradiol (E2) protects several non-reproductive tissues from apoptosis, including skeletal muscle. Previously, we showed that E2 at physiological concentrations prevented apoptosis induced by H2O2 in skeletal myoblasts. As we have also demonstrated a clear beneficial action of this hormone on skeletal muscle mitochondria, the present work further characterizes the signaling mechanisms modulated by E2 that are involved in mitochondria protection, which ultimately result in antiapoptosis. Here, we report that E2 through estrogen receptors (ERs) inhibited the H2O2-induced PKCδ and JNK activation, which results in the inhibition of phosphorylation and translocation to mitochondria of the adaptor protein p66Shc. In conjunction, the inhibition by the hormone of this H2O2-triggered signaling pathway results in protection of mitochondrial potential membrane. Our results provide basis for a putative mechanism by which E2 exerts beneficial effects on mitochondria, against oxidative stress, in skeletal muscle cells. J. Cell. Biochem. 116: 1454-1465, 2015.Fil: la Colla, Anabela Belén. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico Bahia Blanca. Instituto de Ciencias Biologicas y Biomedicas del Sur; ArgentinaFil: Boland, Ricardo Leopoldo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico Bahia Blanca. Instituto de Ciencias Biologicas y Biomedicas del Sur; ArgentinaFil: Vasconsuelo, Andrea Anahi. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico Bahia Blanca. Instituto de Ciencias Biologicas y Biomedicas del Sur; Argentin

The Effect of Pre-Analytical Variability on the Measurement of MRM-MS-Based Mid- to High-Abundance Plasma Protein Biomarkers and a Panel of Cytokines

Blood sample processing and handling can have a significant impact on the stability and levels of proteins measured in biomarker studies. Such pre-analytical variability needs to be well understood in the context of the different proteomics platforms available for biomarker discovery and validation. In the present study we evaluated different types of blood collection tubes including the BD P100 tube containing protease inhibitors as well as CTAD tubes, which prevent platelet activation. We studied the effect of different processing protocols as well as delays in tube processing on the levels of 55 mid and high abundance plasma proteins using novel multiple-reaction monitoring-mass spectrometry (MRM-MS) assays as well as 27 low abundance cytokines using a commercially available multiplexed bead-based immunoassay. The use of P100 tubes containing protease inhibitors only conferred proteolytic protection for 4 cytokines and only one MRM-MS-measured peptide. Mid and high abundance proteins measured by MRM are highly stable in plasma left unprocessed for up to six hours although platelet activation can also impact the levels of these proteins. The levels of cytokines were elevated when tubes were centrifuged at cold temperature, while low levels were detected when samples were collected in CTAD tubes. Delays in centrifugation also had an impact on the levels of cytokines measured depending on the type of collection tube used. Our findings can help in the development of guidelines for blood collection and processing for proteomic biomarker studies

Dietary fish oil supplements depress milk fat yield and alter milk fatty acid composition in lactating cows fed grass silage-based diets

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Fish Oil Increases the Duodenal Flow of Long Chain Polyunsaturated Fatty Acids and trans-11 18:1 and Decreases 18:0 in Steers via Changes in the Rumen Bacterial Community

Ruminant fat is rich in SFA, partly due to the biohydrogenation of dietary PUFA to SFA in the rumen. This process can be inhibited by the dietary inclusion of fish oil. The only bacteria isolated from the rumen capable of converting PUFA to SFA are closely related to Clostridium proteoclasticum. The aim of this study was to investigate if a correlation could be found in vivo between dietary fish oil inclusions and the composition of the ruminal bacterial community and specifically of C. proteoclasticum. Six Hereford × Friesian steers, prepared with ruminal and duodenal cannulae, received grass silage plus 1 of 3 concentrates resulting in total dietary fish oil contents of 0, 1, or 3% of dry matter. A dual flow marker technique was employed to estimate the relative flow of fatty acids. Steers fed the 3% fish oil diet had 100% increases in trans 18:1 flow, whereas 18:0 flow declined to 39% of steers fed the control diet. 16S ribosomal RNA-based denaturing gradient gel electrophoresis profiles obtained from ruminal digesta showed major changes in the bacterial community within steers fed the 3% fish oil diet. Quantitative PCR indicated only a weak relation between numbers of C. proteoclasticum and 18:0 flow between treatments and in individual steers (P < 0.05, but the percentage variance accounted for only 22.8) and did not provide unambiguous evidence that numbers of C. proteoclasticum in the rumen dictate the ratios of SFA:PUFA available for absorption by the animal. Understanding which microbes biohydrogenate PUFA in the rumen is key to developing novel strategies to improve the quality of ruminant products

IMPLEMENTASI PROGRAM KELUARGA HARAPAN (PKH) DALAM MENGENTASKAN KEMISKINAN DI KOTA SAMARINDA

Novia Andriani Ahnadi, 2019 Program Keluarga Harapan (PKH) is a conditional assistance program that is given to very poor households with the requirements of the health and education component. This program began in 2007 which was later announced as a national program in 2012. In this study the analytical tool used to test is hypothesis testing using the Chi Square method, this method is used to see the effect of PKH to become a government solution in alleviating community poverty through education and health facilities.The results of this study are that there is a very strong relationship between poor household groups with the help of PKH or it can be said that PKH can be a government solution to alleviate community poverty through education and health facilities with sigma exact values is 0.815 then greater than the critical point of 0.05 (0.815 0.05). The implementation of PKH activities on faculties and health facilities from 2010 - 2017 showed a change in the positive attitudes of PKH participants and there was also a decrease in the number of poor people within that year. Where there was a decrease in the level of poor people in Kota Samarinda from 2010 to 2017 there was a decline in the poor population by 18.02%.

IMPLEMENTASI PROGRAM KELUARGA HARAPAN (PKH) DALAM MENGENTASKAN KEMISKINAN DI KOTA SAMARINDA

Novia Andriani Ahnadi, 2019 Program Keluarga Harapan (PKH) is a conditional assistance program that is given to very poor households with the requirements of the health and education component. This program began in 2007 which was later announced as a national program in 2012. In this study the analytical tool used to test is hypothesis testing using the Chi Square method, this method is used to see the effect of PKH to become a government solution in alleviating community poverty through education and health facilities.The results of this study are that there is a very strong relationship between poor household groups with the help of PKH or it can be said that PKH can be a government solution to alleviate community poverty through education and health facilities with sigma exact values is 0.815 then greater than the critical point of 0.05 (0.815> 0.05). The implementation of PKH activities on faculties and health facilities from 2010 - 2017 showed a change in the positive attitudes of PKH participants and there was also a decrease in the number of poor people within that year. Where there was a decrease in the level of poor people in Kota Samarinda from 2010 to 2017 there was a decline in the poor population by 18.02%