32 research outputs found

    Complicaciones neurológicas tras el tratamiento láser en el Síndrome de Transfusión Feto-fetal: una revisión sistemática

    Get PDF
    Memoria del Trabajo de Fin de Grado del curso 2016-2017, expuesto y entregado en la Facultad de Medicina de la Universidad de Málaga en junio de 2017 (archivo en PDF). Con Licencia Creative Commons – Reconocimiento – NoComercial – SinObraDerivada (LCC-by-nc-nd). Trabajo adaptado, ampliado y traducido por la autora y sus directores y enviado como preprint a la revista Journal of Perinatal Medicine, con el título: Neurological complications after therapy for fetal-fetal transfusion syndrome: a systematic review of the outcomes at 24 month. Aceptado y prepublicado online con el doi: 10.1515/jpm-2017-0217. Cita en formato Vancouver de este trabajo (para añadir fecha de consulta y URL/handle): Miralles-Gutiérrez A. Complicaciones neurológicas tras el tratamiento láser en el Síndrome de Transfusión Feto-fetal: una revisión sistemática [Trabajo de Fin de Grado]. Universidad de Málaga, Facultad de Medicina; 2017 [citado ___ ]. Disponible en: http://___RESUMEN Objetivo: El principal objetivo de este trabajo se centra en recopilar la evidencia científica disponible sobre la existencia de consecuencias o lesiones neurológicas a medio-largo plazo en gemelos afectos de Síndrome de Transfusión Feto-Fetal (STFF) tras el tratamiento con terapia laser. Método: Se ha realizado una revisión sistemática de los estudios comprendidos entre 2009 y enero de 2017 sobre el daño neurológico (cognición, desarrollo motor, habilidad comunicativa y parálisis cerebral) en gemelos tratados con terapia laser. Se han empleado como criterios la evaluación a los 2 años de edad y el uso de escalas Bailey y ASQ. La búsqueda electrónica y manual identificó 25 artículos, de los cuales 9 fueron incluidos para el análisis. Resultados: La tasa media global de afectación neurológica en el tratamiento láser fue de 14,07%. La tasa media de afectación cognitiva fue de 8,41%; de afectación motora un 11,14%; de afectación comunicativa fue de 16,5% y la de parálisis cerebral fue de 5,73%. Estos resultados fueron mayores que las tasas evaluadas en gemelos dicoriónicos, pero menores que las tasas evaluadas en amniorreducción y el tratamiento conservador. Conclusión: La técnica láser muestra una menor tasa de afectación neurológica a los 2 años de edad en comparación con otras técnicas terapéuticas, pero ésta sigue siendo mayor que las tasas obtenidas para gemelos dicoriónicos. Para confirmar estos hallazgos en la edad de evaluación y en edades posteriores son necesarios estudios protocolizados a largo plazo

    Psychosocial Impact of the COVID-19 Pandemic on Healthcare Professionals in Spain

    Get PDF
    In December 2019, a new virus called SARS-CoV-2 appeared, and the disease it produced was named COVID-19. After the pandemic situation was declared by the World Health Organization in March 2020, an increasing worsening of the mental health of the population, especially healthcare professionals, became apparent. To determine the psychosocial impact of the pandemic on healthcare professionals in Spain, a longitudinal study was conducted at two time points separated by 5 weeks (n time 1 = 169; n time 2 = 65). Participants completed an online questionnaire assessing depression, anxiety, and stress (Depression Anxiety Stress Scale, DASS-21), burnout (Maslach Burnout Inventory—MBI), resilience (Brief Resilience Scale—BRS), and health-related quality of life (SF-36 Health Questionnaire). The results showed high levels of depression, anxiety, stress, and burnout, as well as moderate levels of resilience and low levels of quality of life at the first assessment. Resilience was negatively related to negative experiences, and positively related to the quality of life, also at the first assessment. Overall, there was an improvement in the mental health of healthcare professionals when comparing the two assessments. In this improvement, having COVID-19 in the first assessment had a positive effect on perceived mental health in the second assessment

    A pair of non-optimal codons are necessary for the correct biosynthesis of the Aspergillus nidulans urea transporter, UreA

    Get PDF
    In both prokaryotic and eukaryotic genomes, synonymous codons are unevenly used. Such differential usage of optimal or non-optimal codons has been suggested to play a role in the control of translation initiation and elongation, as well as at the level of transcription and mRNA stability. In the case of membrane proteins, codon usage has been proposed to assist in the establishment of a pause necessary for the correct targeting of the nascent chains to the translocon. By using as a model UreA, the Aspergillus nidulans urea transporter, we revealed that a pair of non-optimal codons encoding amino acids situated at the boundary between the N-terminus and the first transmembrane segment are necessary for proper biogenesis of the protein at 37°C. These codons presumably regulate the translation rate in a previously undescribed fashion, possibly contributing to the correct interaction of ureA-translating ribosome-nascent chain complexes with the signal recognition particle and/or other factors, while the polypeptide has not yet emerged from the ribosomal tunnel. Our results suggest that the presence of the pair of non-optimal codons would not be functionally important in all cellular conditions. Whether this mechanismwould affect other proteins remains to be determined

    Expansion of Signal Transduction Pathways in Fungi by Extensive Genome Duplication

    Get PDF
    Plants and fungi use light and other signals to regulate development, growth, and metabolism. The fruiting bodies of the fungus Phycomyces blakesleeanus are single cells that react to environmental cues, including light, but the mechanisms are largely unknown [1]. The related fungus Mucor circinelloides is an opportunistic human pathogen that changes its mode of growth upon receipt of signals from the environment to facilitate pathogenesis [2]. Understanding how these organisms respond to environmental cues should provide insights into the mechanisms of sensory perception and signal transduction by a single eukaryotic cell, and their role in pathogenesis. We sequenced the genomes of P. blakesleeanus and M. circinelloides and show that they have been shaped by an extensive genome duplication or, most likely, a whole-genome duplication (WGD), which is rarely observed in fungi [3–6]. We show that the genome duplication has expanded gene families, including those involved in signal transduction, and that duplicated genes have specialized, as evidenced by differences in their regulation by light. The transcriptional response to light varies with the developmental stage and is still observed in a photoreceptor mutant of P. blakesleeanus. A phototropic mutant of P. blakesleeanus with a heterozygous mutation in the photoreceptor gene madA demonstrates that photosensor dosage is important for the magnitude of signal transduction. We conclude that the genome duplication provided the means to improve signal transduction for enhanced perception of environmental signals. Our results will help to understand the role of genome dynamics in the evolution of sensory perception in eukaryotes.Office of Science (USA) DE-AC02-05CH11231Ministerio de Economía y Competitividad BIO2005-25029-E , BIO2015-67148-RJunta de Andalucía P06-CVI-0165

    The state of the Martian climate

    Get PDF
    60°N was +2.0°C, relative to the 1981–2010 average value (Fig. 5.1). This marks a new high for the record. The average annual surface air temperature (SAT) anomaly for 2016 for land stations north of starting in 1900, and is a significant increase over the previous highest value of +1.2°C, which was observed in 2007, 2011, and 2015. Average global annual temperatures also showed record values in 2015 and 2016. Currently, the Arctic is warming at more than twice the rate of lower latitudes

    Procediment d'higiene de mans

    Get PDF
    Higiene de mans; Mesures de prevenció; Antisèpsia de mans; InfeccionsHigiene de manos; Medidas de prevención; Antisepsia de manos; InfeccionesHand hygiene; Prevention measures; Hand antisepsis; InfectionsL’objectiu d'aquest document és fer un treball de revisió, actualització i consens de diversos documents sobre la higiene de mans en l'àmbit hospitalari i a l'atenció primària, per crear-ne un de sol que faciliti la informació i la consulta als professionals, amb la finalitat de millorar el seu compliment de la higiene de mans

    Expansion of Signal Transduction Pathways in Fungi by Extensive Genome Duplication

    Get PDF
    [EN] Plants and fungi use light and other signals to regulate development, growth, and metabolism. The fruiting bodies of the fungus Phycomyces blakesleeanus are single cells that react to environmental cues, including light, but the mechanisms are largely unknown [1]. The related fungus Mucor circinelloides is an opportunistic human pathogen that changes its mode of growth upon receipt of signals from the environment to facilitate pathogenesis [2]. Understanding how these organisms respond to environmental cues should provide insights into the mechanisms of sensory perception and signal transduction by a single eukaryotic cell, and their role in pathogenesis. We sequenced the genomes of P. blakesleeanus and M. circinelloides and show that they have been shaped by an extensive genome duplication or, most likely, a whole-genome duplication (WGD), which is rarely observed in fungi [3-6]. We show that the genome duplication has expanded gene families, including those involved in signal transduction, and that duplicated genes have specialized, as evidenced by differences in their regulation by light. The transcriptional response to light varies with the developmental stage and is still observed in a photoreceptor mutant of P. blakesleeanus. A phototropic mutant of P. blakesleeanus with a heterozygous mutation in the photoreceptor gene madA demonstrates that photosensor dosage is important for the magnitude of signal transduction. We conclude that the genome duplication provided the means to improve signal transduction for enhanced perception of environmental signals. Our results will help to understand the role of genome dynamics in the evolution of sensory perception in eukaryotes.European funds (European Regional Development Fund, ERDF); Spanish Ministerio de Economı´a y Competitividad; Junta de Andalucí

    HTLV-1 infection in solid organ transplant donors and recipients in Spain

    Get PDF
    HTLV-1 infection is a neglected disease, despite infecting 10-15 million people worldwide and severe illnesses develop in 10% of carriers lifelong. Acknowledging a greater risk for developing HTLV-1 associated illnesses due to immunosuppression, screening is being widely considered in the transplantation setting. Herein, we report the experience with universal HTLV testing of donors and recipients of solid organ transplants in a survey conducted in Spain. All hospitals belonging to the Spanish HTLV network were invited to participate in the study. Briefly, HTLV antibody screening was performed retrospectively in all specimens collected from solid organ donors and recipients attended since the year 2008. A total of 5751 individuals were tested for HTLV antibodies at 8 sites. Donors represented 2312 (42.2%), of whom 17 (0.3%) were living kidney donors. The remaining 3439 (59.8%) were recipients. Spaniards represented nearly 80%. Overall, 9 individuals (0.16%) were initially reactive for HTLV antibodies. Six were donors and 3 were recipients. Using confirmatory tests, HTLV-1 could be confirmed in only two donors, one Spaniard and another from Colombia. Both kidneys of the Spaniard were inadvertently transplanted. Subacute myelopathy developed within 1 year in one recipient. The second recipient seroconverted for HTLV-1 but the kidney had to be removed soon due to rejection. Immunosuppression was stopped and 3 years later the patient remains in dialysis but otherwise asymptomatic. The rate of HTLV-1 is low but not negligible in donors/recipients of solid organ transplants in Spain. Universal HTLV screening should be recommended in all donor and recipients of solid organ transplantation in Spain. Evidence is overwhelming for very high virus transmission and increased risk along with the rapid development of subacute myelopathy
    corecore