Competitividad local de la horticultura en Santa Maria JaJalpa, municipio de Tenango Del valle, Estado de Mexico

La horticultura en Santa Maria Jajalpa no alcanza el nivel de competitividad exigido por el mercado nacional e internacional. Sin embargo los productores utilizan los recursos naturales disponibles y los ingresos aportados por el trabajo externo para mantener la producción de hortalizas. Esta actividad económica es desarrollada por los integrantes de la familia y peones, la producción se destina al consumo y para la venta en el mercado local y a los intermediarios. En el estudio se analizan fuentes estadísticas, documentales y la información obtenida por medio de un cuestionario aplicado en unidades de producción seleccionadas.La horticultura en Santa Maria Jajalpa no alcanza el nivel de competitividad exigido por el mercado nacional e internacional. Sin embargo los productores utilizan los recursos naturales disponibles y los ingresos aportados por el trabajo externo para mantener la producción de hortalizas. Esta actividad económica es desarrollada por los integrantes de la familia y peones, la producción se destina al consumo y para la venta en el mercado local y a los intermediarios. En el estudio se analizan fuentes estadísticas, documentales y la información obtenida por medio de un cuestionario aplicado en unidades de producción seleccionadas

The role of iron-reducing bacteria in the corrosion of carbon steel: a new microbiologically influenced corrosion mechanism

It was not too long ago that the ability of microorganisms to capture energy by engaging in electron transfer reactions with extracellular solid materials was discovered. This discovery has shifted the paradigms regarding the requirements for life and has prompted tremendous research interest in the challenges and opportunities this type of metabolism poses for humankind. Possibly, in no other field does the microbial extracellular electron transfer reactions have more direct implications today than in the corrosion of steel infrastructure. Due to the strong dependence of our society on iron and its alloys, understanding how microorganisms influence the corrosion of these materials through extracellular electron transfer reactions becomes a matter of substantial importance for industries across the globe. In this dissertation, I aimed to answer how the iron-reducing bacterium Shewanella oneidensis influences the corrosion of carbon steel. It was in this organism where researchers witnessed for the first time the ability of microorganisms to transfer electrons to extracellular substrates, and since then, S. oneidensis has become a model for the understanding of microbial extracellular electron transfer reactions. Because Shewanella spp. are also frequently found in steel infrastructure undergoing corrosion, the next logical question to address is whether or not Shewanella spp. could use extracellular electron uptake to accelerate the corrosion of steel. Addressing the aforementioned questions was the goal in the making of this dissertation. In Chapter 1, I will introduce my motivation to pursue these fascinating research questions, present my hypotheses and goals, and provide a literature review on the ecophysiology of the genus Shewanella, our current understanding of the microbiologically influenced corrosion of steel, and the existing knowledge on the role that iron-reducing bacteria play in the corrosion of carbon steel. In Chapter 2, I evaluate the ability of S. oneidensis to accelerate the corrosion of carbon steel by removing the iron oxide passivating layer through dissimilatory iron reduction, and I test the hypothesis that the presence of short-chain deprotonated dicarboxylic acids would exacerbate corrosion driven by S. oneidensis by accelerating the dissolution of ferric iron and increasing the microbial iron respiration rates. I found that the short-chain deprotonated dicarboxylic acids oxalate, malonate, and succinate accelerate the corrosion of carbon steel driven by S. oneidensis up to 2.6 times more relative to the sterile control experiment without dicarboxylates. The three deprotonated dicarboxylic acids tested enhanced the dissolution of ferric iron, but interestingly this did not result in increased iron respiration rates. My results suggest that a complex array of competing biological (e.g., microbial iron reduction), chemical (e.g., ligand-assisted iron dissolution), and physical (e.g., adsorption of corrosion products) processes drive the accelerated corrosion of carbon steel by iron-reducing bacteria in the presence of iron-binding ligands. In Chapter 3, I offer a novel approach to test the ability of microorganisms to take electrons directly from carbon steel while evaluating the role of hydrogen consumption metabolism and direct electron uptake in the corrosion of carbon steel driven by S. oneidensis. I performed experiments with carbon steel and a S. oneidensis strain incapable of consuming hydrogen and a strain incapable of engaging in direct electron uptake. The results showed that S. oneidensis accelerates the corrosion of carbon steel up to four times more when compared to abiotic experiments and that direct electron uptake is the most significant corrosion mechanism in S. oneidensis. Finally, in Chapter 4, I offer my insights on the new research questions that emerge based on the findings of this dissertation and provide my ideas on the contributions and the limitations of my Ph.D. research

Identification of novel transcriptional regulators involved in macrophage differentiation and activation in U937 cells

Background Monocytes and macrophages play essential role in innate immunity. Understanding the underlying mechanism of macrophage differentiation and the identification of regulatory mechanisms will help to find new strategies to prevent their harmful effects in chronic inflammatory diseases and sepsis. Results Maturation of blood monocytes into tissue macrophages and subsequent inflammatory response was mimicked in U937 cells of human histocytic lymphoma origin. Whole genome array analysis was employed to evaluate gene expression profile to identify underlying transcriptional networks implicated during the processes of differentiation and inflammation. In addition to already known transcription factors (i.e. MAFB, EGR, IRF, BCL6, NFkB, AP1, Nur77), gene expression analysis further revealed novel genes (i.e. MEF2, BRI, HLX, HDAC5, H2AV, TCF7L2, NFIL3) previously uncharacterized to be involved in the differentiation process. A total of 58 selected genes representing cytokines, chemokines, surface antigens, signaling molecules and transcription factors were validated by real time PCR and compared to primary monocyte-derived macrophages. Beside the verification of several new genes, the comparison reveals individual heterogeneity of blood donors. Conclusion Up regulation of MEF2 family, HDACs, and H2AV during cell differentiation and inflammation sheds new lights onto regulation events on transcriptional and epigenetic level controlling these processes. Data generated will serve as a source for further investigation of macrophages differentiation pathways and related biological responses

Enfrentando los riesgos socionaturales

El objetivo del libro es comprender la magnitud de los Riesgos Socionaturales en México y Latinoamérica, para comprender el peligro que existe por algún tipo de desastre, ya sea inundaciones, sismos, remoción en masa, entre otros, además conocer qué medidas preventivas, correctivas y de contingencias existen para estar atentos ante alguna señal que la naturaleza esté enviando y así evitar alguna catástrofe. El libro se enfoca en los aspectos básicos de análisis de los peligros, escenarios de riesgo, vulnerabilidad y resiliencia, importantes para la gestión prospectiva o preventiva

The Fourteenth Data Release of the Sloan Digital Sky Survey: First Spectroscopic Data from the extended Baryon Oscillation Spectroscopic Survey and from the second phase of the Apache Point Observatory Galactic Evolution Experiment

The fourth generation of the Sloan Digital Sky Survey (SDSS-IV) has been in operation since July 2014. This paper describes the second data release from this phase, and the fourteenth from SDSS overall (making this, Data Release Fourteen or DR14). This release makes public data taken by SDSS-IV in its first two years of operation (July 2014-2016). Like all previous SDSS releases, DR14 is cumulative, including the most recent reductions and calibrations of all data taken by SDSS since the first phase began operations in 2000. New in DR14 is the first public release of data from the extended Baryon Oscillation Spectroscopic Survey (eBOSS); the first data from the second phase of the Apache Point Observatory (APO) Galactic Evolution Experiment (APOGEE-2), including stellar parameter estimates from an innovative data driven machine learning algorithm known as "The Cannon"; and almost twice as many data cubes from the Mapping Nearby Galaxies at APO (MaNGA) survey as were in the previous release (N = 2812 in total). This paper describes the location and format of the publicly available data from SDSS-IV surveys. We provide references to the important technical papers describing how these data have been taken (both targeting and observation details) and processed for scientific use. The SDSS website (www.sdss.org) has been updated for this release, and provides links to data downloads, as well as tutorials and examples of data use. SDSS-IV is planning to continue to collect astronomical data until 2020, and will be followed by SDSS-V.Comment: SDSS-IV collaboration alphabetical author data release paper. DR14 happened on 31st July 2017. 19 pages, 5 figures. Accepted by ApJS on 28th Nov 2017 (this is the "post-print" and "post-proofs" version; minor corrections only from v1, and most of errors found in proofs corrected

Recovering the origins of the lenticular galaxy NGC 3115 using multiband imaging

A detailed study of the morphology of lenticular galaxies is an important way to understand how this type of galaxy is formed and evolves over time. Decomposing a galaxy into its components (disc, bulge, bar, ...) allows recovering the colour gradients present in each system, its star formation history, and its assembly history. We use GALFITM to perform a multiwavelength structural decomposition of the closest lenticular galaxy, NGC 3115, resulting in the description of its stellar light into several main components: a bulge, a thin disc, a thick disc, and also evidence of a bar. We report the finding of central bluer stellar populations in the bulge, as compared to the colour of the galaxy outskirts, indicating either the presence of an active galactic nucleus (AGN) and/or recent star formation activity. From the spectral energy distribution results, we show that the galaxy has a low luminosity AGN component, but even excluding the effect of the nuclear activity, the bulge is still bluer than the outer-regions of the galaxy, revealing a recent episode of star formation. Based on all of the derived properties, we propose a scenario for the formation of NGC 3115 consisting of an initial gas-rich merger, followed by accretions and feedback that quench the galaxy, until a recent encounter with the companion KK084 that reignited the star formation in the bulge, provoked a core displacement in NGC 3115 and generated spiral-like features. This result is consistent with the two-phase formation scenario, proposed in previous studies of this galaxy

Genome-wide association study of inhaled corticosteroid response in admixed children with asthma

Background Inhaled corticosteroids (ICS) are the most widely prescribed and effective medication to control asthma symptoms and exacerbations. However, many children still have asthma exacerbations despite treatment, particularly in admixed populations, such as Puerto Ricans and African Americans. A few genome‐wide association studies (GWAS) have been performed in European and Asian populations, and they have demonstrated the importance of the genetic component in ICS response. Objective We aimed to identify genetic variants associated with asthma exacerbations in admixed children treated with ICS and to validate previous GWAS findings. Methods A meta‐analysis of two GWAS of asthma exacerbations was performed in 1347 admixed children treated with ICS (Hispanics/Latinos and African Americans), analysing 8.7 million genetic variants. Those with P ≤ 5 × 10−6 were followed up for replication in 1697 asthmatic patients from six European studies. Associations of ICS response described in published GWAS were followed up for replication in the admixed populations. Results A total of 15 independent variants were suggestively associated with asthma exacerbations in admixed populations (P ≤ 5 × 10−6). One of them, located in the intergenic region of APOBEC3B and APOBEC3C, showed evidence of replication in Europeans (rs5995653, P = 7.52 × 10−3) and was also associated with change in lung function after treatment with ICS (P = 4.91 × 10−3). Additionally, the reported association of the L3MBTL4‐ARHGAP28 genomic region was confirmed in admixed populations, although a different variant was identified. Conclusions and clinical relevance This study revealed the novel association of APOBEC3B and APOBEC3C with asthma exacerbations in children treated with ICS and replicated previously identified genomic regions. This contributes to the current knowledge about the multiple genetic markers determining responsiveness to ICS which could lead in the future the clinical identification of those asthma patients who are not able to respond to such treatment

Multi-ancestry genome-wide association study of asthma exacerbations

Altres ajuts: European Regional Development Fund "ERDF A way of making Europe"; Allergopharma-EAACI award 2021; SysPharmPedia grant from the ERACoSysMed 1st Joint Transnational Call from the European Union under the Horizon 2020; Sandler Family Foundation; American Asthma Foundation; RWJF Amos Medical Faculty Development Program; National Heart, Lung, and Blood Institute of the National Institutes of Health (R01HL117004, R01HL128439, R01HL135156, X01HL134589, R01HL141992, R01HL141845); National Institute of Health and Environmental Health Sciences (R01ES015794, R21ES24844); National Institute on Minority Health and Health Disparities (NIMHD) (P60MD006902, R01MD010443, R56MD013312); National Institute of General Medical Sciences (NIGMS) (RL5GM118984); Tobacco-Related Disease Research Program (24RT-0025, 27IR-0030); National Human Genome Research Institute (NHGRI) (U01HG009080); GlaxoSmithKline and Utrecht Institute for Pharmaceutical Sciences; Slovenian Research Agency (P3-0067); SysPharmPediA grant, co-financed by the Ministry of Education, Science and Sport Slovenia (MIZS) (C3330-16-500106); NHS Research Scotland; Wellcome Trust Biomedical Resource (099177/Z/12/Z); Genotyping National Centre (CeGEN) CeGen-PRB3-ISCIII (AC15/00015); UK Medical Research Council and Wellcome (102215/2/13/2); University of Bristol; Swedish Heart-Lung Foundation, Swedish Research Council; Region Stockholm (ALF project and database maintenance); NHS Chair of Pharmacogenetics via the UK Department of Health; Innovative Medicines Initiative (IMI) (115010); European Federation of Pharmaceutical Industries and Associations (EFPIA); Spanish National Cancer Research Centre; Fundación Canaria Instituto de Investigación Sanitaria de Canarias (PIFIISC19/17); Erasmus Medical Center; Erasmus University Rotterdam; Netherlands Organization for the Health Research and Development (ZonMw); the Research Institute for Diseases in the Elderly (RIDE); Ministry of Education, Culture and Science; Ministry for Health, Welfare and Sports; European Commission (DG XII); Municipality of Rotterdam; German Federal Ministry of Education and Research (Bundesministerium für Bildung und Forschung, BMBF); U.S. National Institutes of Health (HL07966); European Social Fund "ESF Investing in your future"; Ministerio de Ciencia, Innovación y Universidades; Universidad de La Laguna (ULL); European Academy of Allergy and Clinical Immunology (EAACI); European Respiratory Society (ERS) (LTRF202101-00861); Ministry of Education, Science and Sport of the Republic of Slovenia (C3330-19-252012); Singapore Ministry of Education Academic Research Fund; Singapore Immunology Network (SIgN); National Medical Research Council (NMRC Singapore); Biomedical Research Council (BMRC Singapore); Agency for Science Technology and Research (A*STAR Singapore, N-154-000-038-001, R-154-000-191-112, R-154-000-404-112, R-154-000-553-112, R-154-000-565-112, R-154-000-630-112, R-154-000-A08-592, R-154-000-A27-597, R-154-000-A91-592, R-154-000-A95-592, R-154-000-B99-114, BMRC/01/1/21/18/077, BMRC/04/1/21/19/315, SIgN-06-006, SIgN-08-020, NMRC/1150/2008, H17/01/a0/008); Sime Darby Technology Centre; First Resources Ltd; Genting Plantation; Olam International; U.S. National Institutes of Health (HL138098).Background: Asthma exacerbations are a serious public health concern due to high healthcare resource utilization, work/school productivity loss, impact on quality of life, and risk of mortality. The genetic basis of asthma exacerbations has been studied in several populations, but no prior study has performed a multi-ancestry meta-analysis of genome-wide association studies (meta-GWAS) for this trait. We aimed to identify common genetic loci associated with asthma exacerbations across diverse populations and to assess their functional role in regulating DNA methylation and gene expression. Methods: A meta-GWAS of asthma exacerbations in 4989 Europeans, 2181 Hispanics/Latinos, 1250 Singaporean Chinese, and 972 African Americans analyzed 9.6 million genetic variants. Suggestively associated variants (p ≤ 5 × 10) were assessed for replication in 36,477 European and 1078 non-European asthma patients. Functional effects on DNA methylation were assessed in 595 Hispanic/Latino and African American asthma patients and in publicly available databases. The effect on gene expression was evaluated in silico. Results: One hundred and twenty-six independent variants were suggestively associated with asthma exacerbations in the discovery phase. Two variants independently replicated: rs12091010 located at vascular cell adhesion molecule-1/exostosin like glycosyltransferase-2 (VCAM1/EXTL2) (discovery: odds ratio (OR) = 0.82, p = 9.05 × 10 and replication: OR = 0.89, p = 5.35 × 10) and rs943126 from pantothenate kinase 1 (PANK1) (discovery: OR = 0.85, p = 3.10 × 10 and replication: OR = 0.89, p = 1.30 × 10). Both variants regulate gene expression of genes where they locate and DNA methylation levels of nearby genes in whole blood. Conclusions: This multi-ancestry study revealed novel suggestive regulatory loci for asthma exacerbations located in genomic regions participating in inflammation and host defense

Whole-Genome Sequencing of Pharmacogenetic Drug Response in Racially Diverse Children with Asthma

RATIONALE: Albuterol, a bronchodilator medication, is the first-line therapy for asthma worldwide. There are significant racial/ethnic differences in albuterol drug response. OBJECTIVES: To identify genetic variants important for bronchodilator drug response (BDR) in racially diverse children. METHODS: We performed the first whole-genome sequencing pharmacogenetics study from 1,441 children with asthma from the tails of the BDR distribution to identify genetic association with BDR. MEASUREMENTS AND MAIN RESULTS: We identified population-specific and shared genetic variants associated with BDR, including genome-wide significant (P \u3c 3.53 × 10 CONCLUSIONS: The lack of minority data, despite a collaboration of eight universities and 13 individual laboratories, highlights the urgent need for a dedicated national effort to prioritize diversity in research. Our study expands the understanding of pharmacogenetic analyses in racially/ethnically diverse populations and advances the foundation for precision medicine in at-risk and understudied minority populations