300 research outputs found

    Consequências Cardiorrespiratórias do Cigarro Eletrônico: Perspectivas Atuais

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    This paper presents a comprehensive review of the cardiovascular impacts of electronic cigarette use, based on a critical analysis of the available scientific literature. The results highlight a variety of significant findings, including immediate acute effects such as increased heart rate and blood pressure after electronic cigarette use, as well as potential long-term adverse effects such as endothelial dysfunction and oxidative stress. While some studies suggest that electronic cigarettes may represent a less harmful alternative to conventional smoking, there is still considerable uncertainty regarding their long-term effects on cardiovascular health. These findings underscore the importance of further research to elucidate underlying mechanisms and inform public health policies and clinical practices related to electronic cigarette use.Este trabalho apresenta uma revisão abrangente dos impactos cardiovasculares do uso de cigarros eletrônicos, com base em uma análise crítica da literatura científica disponível. Os resultados destacam uma variedade de descobertas significativas, incluindo efeitos agudos imediatos, como aumento da frequência cardíaca e pressão arterial após o uso de cigarros eletrônicos, bem como potenciais efeitos adversos a longo prazo, como disfunção endotelial e estresse oxidativo. Embora alguns estudos sugiram que os cigarros eletrônicos podem representar uma alternativa menos prejudicial ao tabagismo convencional, ainda há incerteza considerável sobre seus efeitos a longo prazo na saúde cardiovascular. Essas descobertas destacam a importância de uma pesquisa adicional para elucidar os mecanismos subjacentes e informar políticas de saúde pública e práticas clínicas relacionadas ao uso de cigarros eletrônicos

    Análise clínica e terapêutica do tumor odontogênico: ameloblastoma

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    Introdução: O ameloblastoma é um tumor benigno de crescimento lento e invasivo, geralmente assintomático, sendo descoberto na maioria das vezes, através de exames radiográficos. Objetivo: verificar as evidências científicas acerca da análise clínica e terapêutica do tumor odontogênico ameloblastoma. Metodologia: Trata-se de uma revisão sistemática com abordagem qualitativa, realizada em setembro de 2020 com busca nas bases de dados: BVS, SCIELO, LILACS e MEDLINE, usando a estratégia PICO. Descritores selecionados: Ameloblastoma, Neoplasias Mandibulares, Tumores Odontogênicos, associados ao operador booleano And. Inclusão de estudos entre 2010 a 2022 com textos na íntegra, relevantes e disponível em português, inglês ou espanhol. Exclusão de textos incompletos, repetidos e sem relevância para temática. Resultados e discussão: Foram selecionados 24 estudos para construção da pesquisa. Os tipos de ameloblastomas são: unicístico, multicístico, periférico e o menos frequente ameloblastoma maligno. Os sintomas de apresentação incluem massa submucosa de crescimento lento, dentes com mobilidade, má oclusão, parestesia, dor e aproximadamente 35% dos pacientes podem ser assintomáticos. O diagnóstico é feito através da biópsia das células tumorais. Entretanto, o cirurgião-dentista ao suspeitar de ameloblastoma, após exames de imagem é indicado o encaminhamento para um especialista da área. Quanto ao tratamento, alguns autores indicam intervenção cirúrgica conservadora, e menos agressiva, como curetagem, enucleação, outros indicam uma cirurgia mais radical. Conclusão: O ameloblastoma é um tumor benigno, porém agressivo, de origem odontogênica, seu tratamento pode ser conservador ou radical, isso irá depender da extensão da lesão bem como da experiência do cirurgião-dentista para escolher o melhor tratamento para o paciente

    Novel genes and sex differences in COVID-19 severity

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    [EN] Here, we describe the results of a genome-wide study conducted in 11 939 coronavirus disease 2019 (COVID-19) positive cases with an extensive clinical information that were recruited from 34 hospitals across Spain (SCOURGE consortium). In sex-disaggregated genome-wide association studies for COVID-19 hospitalization, genome-wide significance (P < 5 × 10−8) was crossed for variants in 3p21.31 and 21q22.11 loci only among males (P = 1.3 × 10−22 and P = 8.1 × 10−12, respectively), and for variants in 9q21.32 near TLE1 only among females (P = 4.4 × 10−8). In a second phase, results were combined with an independent Spanish cohort (1598 COVID-19 cases and 1068 population controls), revealing in the overall analysis two novel risk loci in 9p13.3 and 19q13.12, with fine-mapping prioritized variants functionally associated with AQP3 (P = 2.7 × 10−8) and ARHGAP33 (P = 1.3 × 10−8), respectively. The meta-analysis of both phases with four European studies stratified by sex from the Host Genetics Initiative (HGI) confirmed the association of the 3p21.31 and 21q22.11 loci predominantly in males and replicated a recently reported variant in 11p13 (ELF5, P = 4.1 × 10−8). Six of the COVID-19 HGI discovered loci were replicated and an HGI-based genetic risk score predicted the severity strata in SCOURGE. We also found more SNP-heritability and larger heritability differences by age (<60 or ≥60 years) among males than among females. Parallel genome-wide screening of inbreeding depression in SCOURGE also showed an effect of homozygosity in COVID-19 hospitalization and severity and this effect was stronger among older males. In summary, new candidate genes for COVID-19 severity and evidence supporting genetic disparities among sexes are provided.S

    Mortality and pulmonary complications in patients undergoing surgery with perioperative SARS-CoV-2 infection: an international cohort study

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    Background: The impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on postoperative recovery needs to be understood to inform clinical decision making during and after the COVID-19 pandemic. This study reports 30-day mortality and pulmonary complication rates in patients with perioperative SARS-CoV-2 infection. Methods: This international, multicentre, cohort study at 235 hospitals in 24 countries included all patients undergoing surgery who had SARS-CoV-2 infection confirmed within 7 days before or 30 days after surgery. The primary outcome measure was 30-day postoperative mortality and was assessed in all enrolled patients. The main secondary outcome measure was pulmonary complications, defined as pneumonia, acute respiratory distress syndrome, or unexpected postoperative ventilation. Findings: This analysis includes 1128 patients who had surgery between Jan 1 and March 31, 2020, of whom 835 (74·0%) had emergency surgery and 280 (24·8%) had elective surgery. SARS-CoV-2 infection was confirmed preoperatively in 294 (26·1%) patients. 30-day mortality was 23·8% (268 of 1128). Pulmonary complications occurred in 577 (51·2%) of 1128 patients; 30-day mortality in these patients was 38·0% (219 of 577), accounting for 81·7% (219 of 268) of all deaths. In adjusted analyses, 30-day mortality was associated with male sex (odds ratio 1·75 [95% CI 1·28–2·40], p\textless0·0001), age 70 years or older versus younger than 70 years (2·30 [1·65–3·22], p\textless0·0001), American Society of Anesthesiologists grades 3–5 versus grades 1–2 (2·35 [1·57–3·53], p\textless0·0001), malignant versus benign or obstetric diagnosis (1·55 [1·01–2·39], p=0·046), emergency versus elective surgery (1·67 [1·06–2·63], p=0·026), and major versus minor surgery (1·52 [1·01–2·31], p=0·047). Interpretation: Postoperative pulmonary complications occur in half of patients with perioperative SARS-CoV-2 infection and are associated with high mortality. Thresholds for surgery during the COVID-19 pandemic should be higher than during normal practice, particularly in men aged 70 years and older. Consideration should be given for postponing non-urgent procedures and promoting non-operative treatment to delay or avoid the need for surgery. Funding: National Institute for Health Research (NIHR), Association of Coloproctology of Great Britain and Ireland, Bowel and Cancer Research, Bowel Disease Research Foundation, Association of Upper Gastrointestinal Surgeons, British Association of Surgical Oncology, British Gynaecological Cancer Society, European Society of Coloproctology, NIHR Academy, Sarcoma UK, Vascular Society for Great Britain and Ireland, and Yorkshire Cancer Research

    AI is a viable alternative to high throughput screening: a 318-target study

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    : High throughput screening (HTS) is routinely used to identify bioactive small molecules. This requires physical compounds, which limits coverage of accessible chemical space. Computational approaches combined with vast on-demand chemical libraries can access far greater chemical space, provided that the predictive accuracy is sufficient to identify useful molecules. Through the largest and most diverse virtual HTS campaign reported to date, comprising 318 individual projects, we demonstrate that our AtomNet® convolutional neural network successfully finds novel hits across every major therapeutic area and protein class. We address historical limitations of computational screening by demonstrating success for target proteins without known binders, high-quality X-ray crystal structures, or manual cherry-picking of compounds. We show that the molecules selected by the AtomNet® model are novel drug-like scaffolds rather than minor modifications to known bioactive compounds. Our empirical results suggest that computational methods can substantially replace HTS as the first step of small-molecule drug discovery

    Network-based approaches reveal potential therapeutic targets for host-directed antileishmanial therapy driving drug repurposing

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    Leishmania parasites are the causal agent of leishmaniasis, an endemic disease in more than 90 countries worldwide. Over the years, traditional approaches focused on the parasite when developing treatments against leishmaniasis. Despite numerous attempts, there is not yet a universal treatment, and those available have allowed for the appearance of resistance. Here, we propose and follow a host-directed approach that aims to overcome the current lack of treatment. Our approach identifies potential therapeutic targets in the host cell and proposes known drug interactions aiming to improve the immune response and to block the host machinery necessary for the survival of the parasite. We started analyzing transcription factor regulatory networks of macrophages infected with Leishmania major. Next, based on the regulatory dynamics of the infection and available gene expression profiles, we selected potential therapeutic target proteins. The function of these proteins was then analyzed following a multilayered network scheme in which we combined information on metabolic pathways with known drugs that have a direct connection with the activity carried out by these proteins. Using our approach, we were able to identify five host protein-coding gene products that are potential therapeutic targets for treating leishmaniasis. Moreover, from the 11 drugs known to interact with the function performed by these proteins, 3 have already been tested against this parasite, verifying in this way our novel methodology. More importantly, the remaining eight drugs previously employed to treat other diseases, remain as promising yet-untested antileishmanial therapies. IMPORTANCE This work opens a new path to fight parasites by targeting host molecular functions by repurposing available and approved drugs. We created a novel approach to identify key proteins involved in any biological process by combining gene regulatory networks and expression profiles. Once proteins have been selected, our approach employs a multilayered network methodology that relates proteins to functions to drugs that alter these functions. By applying our novel approach to macrophages during the Leishmania infection process, we both validated our work and found eight drugs already approved for use in humans that to the best of our knowledge were never employed to treat leishmaniasis, rendering our work as a new tool in the box available to the scientific community fighting parasites

    CONVIVENDO COM O VITILIGO: IMPACTO DA DOENÇA NA QUALIDADE DE VIDA DAS PESSOAS

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    Introdução:O vitiligo é uma doença caracterizada por máculas hipocrômicas e/ou acrômicas assintomáticas, localizadas ou generalizadas no sistema tegumentar. A despigmentação visível na pele do indivíduo e, usualmente, exposta no cotidiano altera a imagem corporal, impactando na sua qualidade de vida. Objetivo: Avaliar a qualidade de vida das pessoas que convivem com o vitiligo. Método: Trata-se de um estudo transversal realizado com 86 indivíduos com vitiligo. Para a coleta de dados, utilizou-se o questionário Vitiligo-Specific Quality-of-Life Instrument para a qualidade de vida. Resultados: As mulheres (81,4%) em idade adulta (média de 39 anos) com vitiligo apresentaram médio impacto na qualidade de vida, e os fatores que interferiram na qualidade de vida estão relacionados aos cuidados intensificados com a pele, à progressão e à aparência da patologia. Conclusão: A patologia vitiligo interferiu na qualidade de vida dos indivíduos adultos. Os fatores que impactaram na qualidade de vida foram os cuidados intensificados com a pele em atividades de lazer, a preocupação com a progressão da doença e a aparência das manchas hipocrômicas

    LIVING WITH VITILIGO: IMPACT OF THE DISEASE ON THE INDIVIDUALS’ QUALITY OF LIFE

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    Introduction: Vitiligo is a disease characterized by asymptomatic hypochromic and/or achromic macules, localized or generalized in the integumentary system. The visible depigmentation in the skin of the individual and usually exposed in daily life changes the body image, impacting their quality of life. Objective: To evaluate the quality of life of people living with vitiligo. Method: This is a cross-sectional study carried out with 86 individuals with vitiligo. For data collection, the Vitiligo-Specific Quality-of-Life Instrument questionnaire was used for quality of life. Results: Adult women (81.4%) (mean age 39 years old) with vitiligo had a medium impact on quality of life, and the factors that interfered with quality of life are related to intensified skin care, progression, and appearance of the disease. Conclusion: The vitiligo pathology interfered in the quality of life of adults. The factors that impacted quality of life were intensified skin care in leisure activities, the progression of the disease and the appearance of hypochromic spots

    Characterization and molecular epidemiology of Staphylococcus aureus strains resistant to beta-lactams isolated from the milk of cows diagnosed with subclinical mastitis

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    Background and Aim: The term ESKAPE, recognized by the WHO, is an acronym, which refers to the pathogens Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp., which is extremely virulent and multidrug-resistant. Although the term is used to designate nosocomial pathogens, in a milking environment, strains of Methicillin-resistant S. aureus have been isolated from cattle diagnosed with clinical and subclinical mastitis. Resistant strains may be involved in the transfer of genes conferring resistance to beta-lactam antimicrobials among the species of microorganisms related to mastitis etiology. This study aimed to trace the phenotypic and genotypic profiles of susceptibility to beta-lactams in S. aureus isolated from milk of cattle diagnosed with subclinical mastitis obtained from different rural properties located in the North of Minas Gerais State, Brazil. Materials and Methods: Sixteen microorganisms previously identified as S. aureus isolated from milk of cattle diagnosed with subclinical mastitis were submitted to matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF), mass spectrometry, and polymerase chain reaction (PCR) analysis for microbial species confirmation. The S. aureus beta-lactams antimicrobial phenotypic resistance profile was investigated by disk diffusion method. PCR methods were also performed to investigate the S. aureus genotypic beta-lactams resistance profile. For this purpose, blaZ, mecA, mecALGA251, blaOxa23, and blaKPC genes were screened among S. aureus isolates. The genetic diversity of S. aureus by fingerprint random amplified polymorphic DNA (RAPD)-PCR was also performed in this study. Results: All isolates showed phenotypic resistance to at least three beta-lactams, among which was meropenem. None of the isolates tested positive for the genes mecALGA251, blaOxa23, and blaKPC; however, the presence of the genes blaZ and mecA was detected among the isolates. The fingerprint analysis divided isolates into two distinct groups and 15 different subgroups. Despite the presence of clonality among the isolates, the PCR-RAPD analysis unveiled a heterogeneous profile with genetic diversity among the S. aureus isolates. Conclusion: In this study, we identified beta-lactams resistant S. aureus strains isolated from the milk of cows diagnosed with subclinical mastitis. The S. aureus beta-lactams resistance was investigated using a phenotypic and genotypic approach. We believe that molecular epidemiology, improved knowledge, and genetic basis of resistance to beta-lactams might assist in asserting guidelines for better management practices of dealing with subclinical mastitis and mapping of origin of resistant pathogens in the studied Brazilian area

    Importância da identificação humana através de marcas de mordida:: uma revisão da literatura

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    La identificación a través de los arcos dentales es un aspecto de la odontología forense que verifica la individualidad de los elementos de la cavidad oral. Los dientes se diferencian en tamaño, forma, arreglo, desgaste, daño, edad, calidad, cantidad y hábitos del individuo. En este trabajo se realizó una revisión de literatura sobre la importancia de la identificación humana a través de marcas de mordida, enfatizando la importancia del cirujano dentista en el área de la pericia criminal. La investigación se realizó en las bases de datos PubMed, SciELO y BVS con los siguientes descriptores en portugués e inglés: la identificación humana y marcas de mordeduras, mordedura humana y pericial, mordeduras de humanos y expertos importantes arcos dentales, y la prueba pericial, odontología forense y marcas de mordida, odontología y prueba pericial, odontología legal e importancia pericial. Se consideraron los artículos publicados en los últimos 10 años. También se realizaron búsquedas en los periódicos RBOL y en la Revista Brasileira de Criminalística, considerando el mismo período de tiempo. Los artículos cuya pertinencia temática fue confirmada en la lectura completa formaron parte de la revisión, totalizando 22 artículos. En el trabajo realizado, obtuvimos informaciones que muestran la importancia del odontolegista en el ámbito de la pericia criminal, pudiendo hacer uso de varias técnicas para la resolución de casos a través de marcas de mordida humana
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