Ley servir y sus efectos en la mejora del servicio administrativo en la municipalidad distrital de Santa María -año 2017 al 2018-

El panorama y tendencia actual es mejorar la calidad de los servicios en las entidades públicas, en razón a ello se ha expedido nuevas normas con el propósito de que se mejore la atención del servicio y por otro lado, que los servidores públicos mejoren en su calidad de vida en virtud de la ley SERVIR, de allí es que proponemos como Objetivo: Determinar de qué forma la Ley N° 30057, Ley de servidores del Régimen del Servicio Civil, SERVIR mejora el servicio a los usuarios en la Municipalidad Distrital de Santa María entre los años 2017 al 2018. Métodos: Para esta investigación, hemos utilizado el tipo de estudio descriptivo, asimismo, el enfoque es mixto, la población es igual que la muestra, siendo en número de 30 personas (funcionarios administrativos, servidores, abogados especialistas y estudiantes de derecho de la UNJFSC), la muestra sometida a encuesta de manera libre ha manifestado su parecer de acuerdo a las preguntas que se presentaron a través de un cuestionario cuyos reactivos buscan comprobar nuestra pretensión. En esta tesis se analiza si la ley n° 30057, Ley de servidores del Régimen del Servicio Civil, SERVIR, una vez aplicada trae resultados favorables en la atención del público. Resultados: en esta investigación se demuestra que, en efecto al aplicar la Ley N° 30057, Ley de servidores del Régimen del Servicio Civil, SERVIR hay una sustancial mejora en la calidad del servicio y, por tanto, en la atención en la administración pública. Conclusión: En consecuencia, la investigación nos permite aseverar que, de aplicarse la Ley SERVIR de manera integral, habría una mejora tanto en la atención a los usuarios de todos los entes administrativas y los administrados y de igual manera los servidores tendrían una mejora en sus ingresos y que redundaría en su calidad de vid

The BED finger domain protein MIG-39 halts migration of distal tip cells in Caenorhabditis elegans

AbstractOrgans are often formed by the extension and branching of epithelial tubes. An appropriate termination of epithelial tube extension is important for generating organs of the proper size and morphology. However, the mechanism by which epithelial tubes terminate their extension is mostly unknown. Here we show that the BED-finger domain protein MIG-39 acts to stop epithelial tube extension in Caenorhabditis elegans. The gonadal leader cells, called distal tip cells (DTCs), migrate in a U-shaped pattern during larval development and stop migrating at the young adult stage, generating a gonad with anterior and posterior U-shaped arms. In mig-39 mutants, however, DTCs overshot their normal stopping position. MIG-39 promoted the deceleration of DTCs, leading to the proper timing and positioning of the cessation of DTC migration. Among three Rac GTPase genes, mutations in ced-10 and rac-2 enhanced the overshoot of anterior DTCs, while they suppressed that of posterior DTCs of mig-39 mutants. On the other hand, the mutation in mig-2 suppressed both the anterior and posterior DTC defects of mig-39. Genetic analyses suggested that MIG-39 acts in parallel with Rac GTPases in stopping DTC migration. We propose a model in which the anterior and posterior DTCs respond in an opposite manner to the levels of Rac activities in the cessation of DTC migration

Accuracy of wind observations from open-ocean buoys: Correction for flow distortion

The comparison of equivalent neutral winds obtained from (a) four WHOI buoys in the subtropics and (b) scatterometer estimates at those locations reveals a root-mean-square (RMS) difference of 0.56-0.76 m/s. To investigate this RMS difference, different buoy wind error sources were examined. These buoys are particularly well suited to examine two important sources of buoy wind errors because: (1) redundant anemometers and a comparison with numerical flow simulations allow us to quantitatively assess flow distortion errors, and (2) one-minute sampling at the buoys allows us to examine the sensitivity of buoy temporal sampling/averaging in the buoy-scatterometer comparisons. The inter-anemometer difference varies as a function of wind direction relative to the buoy wind vane and is consistent with the effects of flow distortion expected based on numerical flow simulations. Comparison between the anemometers and scatterometer winds supports the interpretation that the inter-anemometer disagreement, which can be up to 5% of the wind speed, is due to flow distortion. These insights motivate an empirical correction to the individual anemometer records and subsequent comparison with scatterometer estimates show good agreement

Controlling transmembrane protein concentration and orientation in supported lipid bilayers

The trans-membrane protein – proteorhodopsin (pR) has been incorporated into supported lipid bilayers (SLB). In-plane electric fields have been used to manipulate the orientation and concentration of these proteins, within the SLB, through electrophoresis leading to a 25-fold increase concentration of pR

Micrometre and nanometre scale patterning of binary polymer brushes, supported lipid bilayers and proteins

Binary polymer brush patterns were fabricated via photodeprotection of an aminosilane with a photo-cleavable nitrophenyl protecting group. UV exposure of the silane film through a mask yields micrometre-scale amine-terminated regions that can be derivatised to incorporate a bromine initiator to facilitate polymer brush growth via atom transfer radical polymerisation (ATRP). Atomic force microscopy (AFM) and imaging secondary ion mass spectrometry (SIMS) confirm that relatively thick brushes can be grown with high spatial confinement. Nanometre-scale patterns were formed by using a Lloyd's mirror interferometer to expose the nitrophenyl-protected aminosilane film. In exposed regions, protein-resistant poly(oligo(ethylene glycol)methyl ether methacrylate) (POEGMEMA) brushes were grown by ATRP and used to define channels as narrow as 141 nm into which proteins could be adsorbed. The contrast in the pattern can be inverted by (i) a simple blocking reaction after UV exposure, (ii) a second deprotection step to expose previously intact protecting groups, and (iii) subsequent brush growth via surface ATRP. Alternatively, two-component brush patterns can be formed. Exposure of a nitrophenyl-protected aminosilane layer either through a mask or to an interferogram, enables growth of an initial POEGMEMA brush. Subsequent UV exposure of the previously intact regions allows attachment of ATRP initiator sites and growth of a second poly(cysteine methacrylate) (PCysMA) brush within photolithographically-defined micrometre or nanometre scale regions. POEGMEMA brushes resist deposition of liposomes, but fluorescence recovery after photobleaching (FRAP) studies confirm that liposomes readily rupture on PCysMA “corrals” defined within POEGMEMA “walls”. This leads to the formation of highly mobile supported lipid bilayers that exhibit similar diffusion coefficients to lipid bilayers formed on surfaces such as glass

Programmable Assembly of DNA-Functionalized Liposomes by DNA

This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano, copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see http://dx.doi.org/10.1021/nn1030093Bionanotechnology involves the use of biomolecules to control both the structure and property of nanomaterials. One of the most studied examples is DNA-directed assembly of inorganic nanoparticles such as gold nanoparticles (AuNPs). However, systematic studies on DNA-linked soft nanoparticles, such as liposomes, are still lacking. We herein report the programmable assembly and systematic characterization of DNA-linked liposomes as a function of liposome size, charge, fluidity, composition, DNA spacer, linker DNA sequence, and salt concentration for direct comparison to DNA-directed assembly of AuNPs. Similar to the assemblies of AuNPs, sharp melting transitions were observed for liposomes where the first derivative of the melting curve full width at half-maximum (fwhm) is equal to or less than 1 °C for all of the tested liposomes, allowing sequence specific DNA detection. We found that parameters such as liposome size, charge, and fluidity have little effect on the DNA melting temperature. Cryo-TEM studies showed that programmable assemblies can be obtained and that the majority of the liposomes maintained a spherical shape in the assembled state. While liposome and AuNP systems are similar in many aspects, there are also important differences that can be explained by their respective physical properties.University of Waterloo || Natural Sciences and Engineering Research Council |

One-pot synthesis of hierarchical porous layered hybrid materials based on aluminosilicate sheets and organic functional pillars

Layered hybrid materials (LHMs) based on ordered silicoaluminate sheets linked with organic fragments, perpendicularly located and stabilized in the interlayer space, were synthesized by a one-pot direct hydrothermal process in the absence of structural directing agents (SDAs) and using bridged silsesquioxanes as organosilicon precursors. By following the synthesis described here, the preliminary preparation of inorganic layered precursors, post-synthesis swelling and/or pillaring treatments can be avoided. The physico-chemical and structural characteristics of the materials were studied by chemical and thermogravimetrical analyses, X-ray diffraction, TEM microscopy, spectroscopic techniques (NMR and FTIR) and textural measurements. The complete exchange of intracrystalline sodium cations by protons, without substantial structural alteration of the hybrid materials, facilitated the generation of hybrid materials, which contained acid and base sites located in the inorganic (silicoaluminate layers) and in the organic interlayer linkers, respectively, with the resultant acid base materials showing promise as active and selective catalysts.The authors thank financial support to Spanish Government by Consolider-Ingenio MULTICAT CSD2009-00050, MAT2011-29020-C02-01 and Severo Ochoa Excellence Program SEV-2012-0267. AG and JMM thank pre-doctoral fellowships from MINECO for economical support.Gaona Cordero, A.; Moreno, JM.; Velty, A.; Díaz Morales, UM.; Corma Canós, A. (2014). One-pot synthesis of hierarchical porous layered hybrid materials based on aluminosilicate sheets and organic functional pillars. 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Fabrication of microstructured binary polymer brush “corrals” with integral pH sensing for studies of proton transport in model membrane systems

Binary brush structures consisting of poly(cysteine methacrylate) (PCysMA) “corrals” enclosed within poly(oligoethylene glycol methyl ether methacrylate) (POEGMA) “walls” are fabricated simply and efficiently using a two-step photochemical process. First, the C–Cl bonds of 4-(chloromethyl)phenylsilane monolayers are selectively converted into carboxylic acid groups by patterned exposure to UV light through a mask and POEGMA is grown from unmodified chlorinated regions by surface-initiated atom-transfer radical polymerisation (ATRP). Incorporation of a ratiometric fluorescent pH indicator, Nile Blue 2-(methacryloyloxy)ethyl carbamate (NBC), into the polymer brushes facilitates assessment of local changes in pH using a confocal laser scanning microscope with spectral resolution capability. Moreover, the dye label acts as a radical spin trap, enabling removal of halogen end-groups from the brushes via in situ dye addition during the polymerisation process. Second, an initiator is attached to the carboxylic acid-functionalised regions formed by UV photolysis in the patterning step, enabling growth of PCysMA brushes by ATRP. Transfer of the system to THF, a poor solvent for PCysMA, causes collapse of the PCysMA brushes. At the interface between the collapsed brush and solvent, selective derivatisation of amine groups is achieved by reaction with excess glutaraldehyde, facilitating attachment of aminobutyl(nitrile triacetic acid) (NTA). The PCysMA brush collapse is reversed on transfer to water, leaving it fully expanded but only functionalized at the brush–water interface. Following complexation of NTA with Ni2+, attachment of histidine-tagged proteorhodopsin and lipid deposition, light-activated transport of protons into the brush structure is demonstrated by measuring the ratiometric response of NBC in the POEGMA walls