Evaluation of genotypic and phenotypic methods for differentiation of the members of the Anginosus group streptococci

The terminology and classification of the Anginosus group streptococci has been inconsistent. We tested the utility of 16S rRNA gene and tuf gene sequencing and conventional biochemical tests for the reliable differentiation of the Anginosus group streptococci. Biochemical testing included Rapid ID 32 Strep, API Strep, Fluo-Card Milleri, Wee-tabs, and Lancefield antigen typing. Altogether, 61 Anginosus group isolates from skin and soft tissue infections and four reference strains were included. Our results showed a good agreement between 16S rRNA gene and tuf gene sequencing. Using the full sequence was less discriminatory than using the first part of the 16S rRNA gene. The three species could not be separated with the API 20 Strep test. Streptococcus intermedius could be differentiated from the other two species by β-galactosidase (ONPG) and β-N-acetyl-glucosaminidase reactions. Rapid ID 32 Strep β-glucosidase reaction was useful in separating S. anginosus strains from S. constellatus. In conclusion, both 16S rRNA gene and tuf gene sequencing can be used for the reliable identification of the Anginosus group streptococci. S. intermedius can be readily differentiated from the other two species by phenotypic tests; however, 16S rRNA gene or tuf gene sequencing may be needed for separating some strains of S. constellatus from S. anginosus

Pre-sorting and pen size effects on the stress responses at loading and unloading and transport losses in market weight pigs

Transport losses represent three challenges for the US swine industry and these are: 1) pig well-being, 2) increased rules and regulations, and 3) direct financial losses to producers and packers. Therefore; improving the well-being of pigs during transport and reducing the incidence of dead and non-ambulatory pigs is a priority for the US swine industry (NPB, 2007). Johnson et al. (2010) studied the effects of grow-finish pen size and manually pre-sorting pigs the day before loading on the stress responses at loading and unloading and transport losses in market weight pigs. The authors reported that pigs loaded from large pens (192 pigs/pen) that were pre-sorted from pen mates had 66% fewer dead and non-ambulatory pigs at the harvest facility compared to pigs loaded from small pens (32 pigs/pen) that were sorted from pen mates during loading. However, it is unclear if the reduction in transport losses was due to pen size and/or pre-sorting before marketing. Therefore, the objectives of the current two research trials were to 1) determine the effects of pre-sorting prior to loading on stress responses and transport losses at the harvest facility in the market weight pig; and 2) determine the effects of grow-finish pen size on stress responses and transport losses at the harvest facility in the market weight pig

Deep neck infection complicating lymphadenitis caused by Streptococcus intermedius in an immunocompetent child

BACKGROUND: Streptococcus intermedius belongs to the Streptococcus anginosus group. It is part of the normal flora of the human mouth, but it can be etiologically associated with deep-site infections. CASE PRESENTATION: We present a case of deep neck infection complicating Streptococcus intermedius lymphadenitis, which developed in an immunocompetent 14-year-old boy with a history of recent dental work. The infection was ultimately eradicated by a combined medical and surgical approach. CONCLUSION: Our report suggests that combined medical and surgical therapy is essential for the complete resolution of deep infections caused by Streptococcus intermedius. Molecular biological techniques can be useful in guiding the diagnostic investigation and providing insight into the possibility of occult abscesses, which are particularly common with Streptococcus intermedius infections

Spatial kinematics of Brightest Cluster Galaxies and their close companions from Integral Field Unit spectroscopy

We present Integral Field Unit (IFU) spectroscopy of four brightest cluster galaxies (BCGs) at z~0.1. Three of the BCGs have close companions within a projected radius of 20 kpc and one has no companion within that radius. We calculate the dynamical masses of the BCGs and their companions to be 1.4x10^11<M_dyn (M_solar)<1.5x10^12. We estimate the probability that the companions of the BCGs are bound using the observed masses and velocity offsets. We show that the lowest mass companion (1:4) is not bound while the two nearly equal mass (1:1.45 and 1:1.25) companions are likely to merge with their host BCGs in 0.35 Gyr in major, dry mergers. We conclude that some BCGs continue to grow from major merging even at z~0. We analyse the stellar kinematics of these systems using the \lambda_R parameter developed by the SAURON team. This offers a new and unique means to measure the stellar angular momentum of BCGs and make a direct comparison to other early-type galaxies. The BCGs and their companions have similar ellipticities to those of other early-type galaxies but are more massive. We find that not all these massive galaxies have low \lambda_R_e as one might expect. One of the four BCGs and the two massive companions are found to be fast-rotating galaxies with high angular momentum, thereby providing a new test for models of galaxy evolution and the formation of Intra-Cluster Light.Comment: 5 pages. Accepted for publication in MNRAS Letter

Systemic long-term metabolic effects of acute non-severe paediatric burn injury

A growing body of evidence supports the concept of a systemic response to non-severe thermal trauma. This provokes an immunosuppressed state that predisposes paediatric patients to poor recovery and increased risk of secondary morbidity. In this study, to understand the long-term systemic effects of non-severe burns in children, targeted mass spectrometry assays for biogenic amines and tryptophan metabolites were performed on plasma collected from child burn patients at least three years post injury and compared to age and sex matched non-burn (healthy) controls. A panel of 12 metabolites, including urea cycle intermediates, aromatic amino acids and quinolinic acid were present in significantly higher concentrations in children with previous burn injury. Correlation analysis of metabolite levels to previously measured cytokine levels indicated the presence of multiple cytokine-metabolite associations in the burn injury participants that were absent from the healthy controls. These data suggest that there is a sustained immunometabolic imprint of non-severe burn trauma, potentially linked to long-term immune changes that may contribute to the poor long-term health outcomes observed in children after burn injury

The relation between stellar populations, structure and environment for dwarf elliptical galaxies from the MAGPOP-ITP

Dwarf galaxies, as the most numerous type of galaxy, offer the potential to study galaxy formation and evolution in detail in the nearby Universe. Although they seem to be simple systems at first view, they remain poorly understood. In an attempt to alleviate this situation, the MAGPOP EU Research and Training Network embarked on a study of dwarf galaxies named MAGPOP-ITP (Peletier et al., 2007). In this paper, we present the analysis of a sample of 24 dwarf elliptical galaxies (dEs) in the Virgo Cluster and in the field, using optical long-slit spectroscopy. We examine their stellar populations in combination with their light distribution and environment. We confirm and strengthen previous results that dEs are, on average, younger and more metal-poor than normal elliptical galaxies, and that their [alpha/Fe] abundance ratios scatter around solar. This is in accordance with the downsizing picture of galaxy formation where mass is the main driver for the star formation history. We also find new correlations between the luminosity-weighted mean age, the large-scale asymmetry, and the projected Virgocentric distance. We find that environment plays an important role in the termination of the star formation activity by ram pressure stripping of the gas in short timescales, and in the transformation of disky dwarfs to more spheroidal objects by harassment over longer timescales. This points towards a continuing infall scenario for the evolution of dEs.Comment: Accepted for publication in MNRAS: 22 pages, 13 figures and 9 table

A mature cluster with X-ray emission at z=2.07

We report evidence of a fully established galaxy cluster at z=2.07, consisting of a ~20sigma overdensity of red, compact spheroidal galaxies spatially coinciding with extended X-ray emission detected with XMM-Newton. We use VLT VIMOS and FORS2 spectra and deep Subaru, VLT and Spitzer imaging to estimate the redshift of the structure from a prominent z=2.07 spectroscopic redshift spike of emission-line galaxies, concordant with the accurate 12-band photometric redshifts of the red galaxies. Using NICMOS and Keck AO observations, we find that the red galaxies have elliptical morphologies and compact cores. While they do not form a tight red sequence, their colours are consistent with that of a >1.3$~Gyr population observed at z~2.1. From an X-ray luminosity of .2*10^43 erg s^-1 and the stellar mass content of the red galaxy population, we estimate a halo mass of 5.3-8*10^13 Msun, comparable to the nearby Virgo cluster. These properties imply that this structure could be the most distant, mature cluster known to date and that X-ray luminous, elliptical-dominated clusters are already forming at substantially earlier epochs than previously known.Comment: 14 pages, 12 figures; accepted for publication in Astronomy & Astrophysic

The evolution of the brightest cluster galaxies since z∼ 1 from the ESO Distant Cluster Survey (EDisCS)

We present K-band data for the brightest cluster galaxies (BCGs) from the ESO Distant Cluster Survey (EDisCS). These data are combined with the photometry published by Aragón-Salamanca, Baugh & Kauffmann and a low-redshift comparison sample built from the BCG catalogue of von der Linden et al. BCG luminosities are measured inside a metric circular aperture with 37 kpc diameter. In agreement with previous studies, we find that the K-band Hubble diagram for BCGs exhibits very low scatter (∼0.35) over a redshift range of 0 2 and evolved passively thereafter. In contrast with some previous studies, we do not detect any significant change in the stellar mass of the BCG since z∼ 1. These results do not seem to depend on the velocity dispersion of the parent cluster. We also find that there is a correlation between the 1D velocity dispersion of the clusters (σcl) and the K-band luminosity of the BCGs (after correcting for passive-evolution). The clusters with large velocity dispersions, and therefore masses, tend to have brighter BCGs, i.e. BCGs with larger stellar masses. This dependency, although significant, is relatively weak: the stellar mass of the BCGs changes only by ∼70 per cent over a two order of magnitude range in cluster mass. Furthermore, this dependency does not change significantly with redshift. We have compared our observational results with the hierarchical galaxy formation and evolution model predictions of De Lucia & Blaizot. We find that the models predict colours which are in reasonable agreement with the observations because the growth in stellar mass is dominated by the accretion of old stars. However, the stellar mass in the model BCGs grows by a factor of 3-4 since z= 1, a growth rate which seems to be ruled out by the observations. The models predict a dependency between the BCG's stellar mass and the velocity dispersion (mass) of the parent cluster in the same sense as the data, but the dependency is significantly stronger than observed. However, one major difficulty in this comparison is that we have measured magnitudes inside a fixed metric aperture while the models compute total luminositie

A novel point-of-care testing strategy for sexually transmitted infections among pregnant women in high-burden settings: Results of a feasibility study in Papua New Guinea

© 2016 The Author(s). Sexually transmitted and genital infections in pregnancy are associated with an increased risk of adverse maternal and neonatal health outcomes. High prevalences of sexually transmitted infections have been identified among antenatal attenders in Papua New Guinea. Papua New Guinea has amongst the highest neonatal mortality rates worldwide, with preterm birth and low birth weight major contributors to neonatal mortality. The overall aim of our study was to determine if a novel point-of-care testing and treatment strategy for the sexually transmitted and genital infections Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Trichomonas vaginalis (TV) and Bacterial vaginosis (BV) in pregnancy is feasible in the high-burden, low-income setting of Papua New Guinea. Methods: Women attending their first antenatal clinic visit were invited to participate. CT/NG and TV were tested using the GeneXpert platform (Cepheid, USA), and BV tested using BVBlue (Gryphus Diagnostics, USA). Participants received same-day test results and antibiotic treatment as indicated. Routine antenatal care including HIV and syphilis screening were provided. Results: Point-of-care testing was provided to 125/222 (56 %) of women attending routine antenatal care during the three-month study period. Among the 125 women enrolled, the prevalence of CT was 20.0 %; NG, 11.2 %; TV, 37.6 %; and BV, 17.6 %. Over half (67/125, 53.6 %) of women had one or more of these infections. Most women were asymptomatic (71.6 %; 47/67). Women aged 24 years and under were more likely to have one or more STI compared with older women (odds ratio 2.38; 95 % CI: 1.09, 5.21). Most women with an STI received treatment on the same day (83.6 %; 56/67). HIV prevalence was 1.6 % and active syphilis 4.0 %. Conclusion: Point-of-care STI testing and treatment using a combination of novel, newly-available assays was feasible during routine antenatal care in this setting. This strategy has not previously been evaluated in any setting and offers the potential to transform STI management in pregnancy and to prevent their associated adverse health outcomes

Discovery of the X-ray selected galaxy cluster XMMU J0338.8+0021 at z = 1.49 - Indications for a young system with a forming brightest galaxy

We report on the discovery of a galaxy cluster at z = 1.490 originally selected as an extended X-ray source in the XMM-Newton Distant Cluster Project. Further observations carried out with the VLT-FORS2 spectrograph allowed the spectroscopic confirmation of seven secure cluster members, providing a median system redshift of z = 1.490 +/- 0.009. The color magnitude diagram of XMMU J0338.8+0021 reveals the presence of a well populated red sequence with z-H ~ 3, albeit with an apparent significant scatter in color. Since we do not detect indications for strong star formation activity in any of the objects, the color spread could indicate different stellar ages of the member galaxies. In addition, we found the brightest cluster galaxy in a very active dynamical state, with an interacting, merging companion located at a physical projected distance of d ~ 20kpc. From the X-ray luminosity we estimate a cluster mass of M200 ~ 1.2 x 10^(14) Msun. The data seem to suggest a scenario in which XMMU J0338.8+0021 is a young system, possibly caught in a moment of active ongoing mass assembly.Comment: 6 pages, 4 figures, 2 Table