Electricity: elektrisk bysykkel i samarbeid med Hard Rocx

In this paper I have cooperated with the bicycle manufacturer Hard Rocx on designing a new electric bike. Hard Rocx is a Norwegian bicycle manufacturer focusing on the development and promotion of carbon bikes for the terrain. In addition they develop road bikes, hybrid bikes, comfort / city bikes and childrens bicycles. I worked closely with the manufacturer for the entire project and together we came up with the concept Electricity. The target group for Electricity is the users in cities and urban areas. In the thesis I have focussed on various design methods and conducting them. Throughout the process my aim has been to retain the values to the manufacturer. I have implemented several different methods during the design of this product. In the early stages I used competitive analysis and in-depth interviews with the manufacturer and potential users. This was to obtain sufficient knowledge about the market and the users which the product was directed to. During concept development I used “moodboards” and “aesthetic guidelines” to visualize different design languages and moods. Furthermore, in idea development stage I used 2D drawing, 3D modeling, 1:1 sketching and mock up, function model and finally an aesthetic modelMaster i produktdesig

Electricity: elektrisk bysykkel i samarbeid med Hard Rocx

In this paper I have cooperated with the bicycle manufacturer Hard Rocx on designing a new electric bike. Hard Rocx is a Norwegian bicycle manufacturer focusing on the development and promotion of carbon bikes for the terrain. In addition they develop road bikes, hybrid bikes, comfort / city bikes and childrens bicycles. I worked closely with the manufacturer for the entire project and together we came up with the concept Electricity. The target group for Electricity is the users in cities and urban areas. In the thesis I have focussed on various design methods and conducting them. Throughout the process my aim has been to retain the values to the manufacturer. I have implemented several different methods during the design of this product. In the early stages I used competitive analysis and in-depth interviews with the manufacturer and potential users. This was to obtain sufficient knowledge about the market and the users which the product was directed to. During concept development I used “moodboards” and “aesthetic guidelines” to visualize different design languages and moods. Furthermore, in idea development stage I used 2D drawing, 3D modeling, 1:1 sketching and mock up, function model and finally an aesthetic mode

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Dynamic regulation of ROCK in tumor cells controls CXCR4-driven adhesion events

CXCR4 is a chemokine receptor often found aberrantly expressed on metastatic tumor cells. To investigate CXCR4 signaling in tumor cell adhesion, we stably overexpressed CXCR4 in MCF7 breast tumor cells. Cell attachment assays demonstrate that stimulation of the receptor with its ligand, CXCL12, promotes adhesion of MCF7-CXCR4 cells to both extracellular matrix and endothelial ligands. To more closely mimic the conditions experienced by a circulating tumor cell, we performed the attachment assays under shear stress conditions. We found that CXCL12-induced tumor cell attachment is much more pronounced under flow. ROCK is a serine/threonine kinase associated with adhesion and metastasis, which is regulated by CXCR4 signaling. Thus, we investigated the contribution of ROCK activity during CXC12-induced adhesion events. Our results demonstrate a biphasic regulation of ROCK in response to adhesion. During the initial attachment, inhibition of ROCK activity is required. Subsequently, re-activation of ROCK activity is required for maturation of adhesion complexes and enhanced tumor cell migration. Interestingly, CXCL12 partially reduces the level of ROCK activity generated by attachment, which supports a model in which stimulation with CXCL12 regulates tumor cell adhesion events by providing an optimal level of ROCK activity for effective migration