Genomic Structure and Chromosomal Location of the Rat Gene Encoding the Zinc Finger Transcription Factor Kid-1

We have previously cloned and sequenced a novel zinc finger cDNA, Kid-1 , from the rat. Because of its developmentally regulated expression pattern and its suppression after renal injury, as well as its kindey-predominant expression, we propose that Kid-1 is likely to play an important role in renal gene regulation. Kid-1 encodes a predicted protein with 13 zinc fingers at the carboxy end and Krüppel-associated box (KRAB) A and B regions at the amino terminus. Expression of a Kid-1-GAL4 chimeric protein results in strong transcriptional repression of cotransfected constructs containing GAL4 binding sites and a chloramphenicol acetyl transferase gene driven by either a minimal promoter or a SV40 enhancer. We now report the cloning, structural organization, and chromosomal localization of the Kid-1 gene. The Kid-1 gene is composed of four exons and three introns, closely reflecting the organization of the Kid-1 protein. The KRAB A and B regions are encoded by the second and third exons, respectively. The entire zinc finger region is encoded by the fourth exon. Using a combination of linkage analysis and somatic cell hybrid analysis, Kid-1was mapped to rat chromosome (RNO) 10. Kid-1, Il3, and Sparc form a tight linkage group on RNO10. Regional sublocalization to RNO10q21.3-q22 was established by fluorescence in situ hybridization.

Magnetohydrodynamic experiments on cosmic magnetic fields

It is widely known that cosmic magnetic fields, i.e. the fields of planets, stars, and galaxies, are produced by the hydromagnetic dynamo effect in moving electrically conducting fluids. It is less well known that cosmic magnetic fields play also an active role in cosmic structure formation by enabling outward transport of angular momentum in accretion disks via the magnetorotational instability (MRI). Considerable theoretical and computational progress has been made in understanding both processes. In addition to this, the last ten years have seen tremendous efforts in studying both effects in liquid metal experiments. In 1999, magnetic field self-excitation was observed in the large scale liquid sodium facilities in Riga and Karlsruhe. Recently, self-excitation was also obtained in the French "von Karman sodium" (VKS) experiment. An MRI-like mode was found on the background of a turbulent spherical Couette flow at the University of Maryland. Evidence for MRI as the first instability of an hydrodynamically stable flow was obtained in the "Potsdam Rossendorf Magnetic Instability Experiment" (PROMISE). In this review, the history of dynamo and MRI related experiments is delineated, and some directions of future work are discussed.Comment: 25 pages, 26 figures, to appear in ZAM

Genomic Structure and Chromosomal Location of the Rat Gene Encoding the Zinc Finger Transcription Factor Kid-1

We have previously cloned and sequenced a novel zinc finger cDNA, Kid-1 , from the rat. Because of its developmentally regulated expression pattern and its suppression after renal injury, as well as its kindey-predominant expression, we propose that Kid-1 is likely to play an important role in renal gene regulation. Kid-1 encodes a predicted protein with 13 zinc fingers at the carboxy end and Krüppel-associated box (KRAB) A and B regions at the amino terminus. Expression of a Kid-1-GAL4 chimeric protein results in strong transcriptional repression of cotransfected constructs containing GAL4 binding sites and a chloramphenicol acetyl transferase gene driven by either a minimal promoter or a SV40 enhancer. We now report the cloning, structural organization, and chromosomal localization of the Kid-1 gene. The Kid-1 gene is composed of four exons and three introns, closely reflecting the organization of the Kid-1 protein. The KRAB A and B regions are encoded by the second and third exons, respectively. The entire zinc finger region is encoded by the fourth exon. Using a combination of linkage analysis and somatic cell hybrid analysis, Kid-1was mapped to rat chromosome (RNO) 10. Kid-1, Il3, and Sparc form a tight linkage group on RNO10. Regional sublocalization to RNO10q21.3-q22 was established by fluorescence in situ hybridization

Reduced Acrolein Detoxification in akr1a1a Zebrafish Mutants Causes Impaired Insulin Receptor Signaling and Microvascular Alterations

Abstract Increased acrolein (ACR), a toxic metabolite derived from energy consumption, is associated with diabetes and its complications. However, the molecular mechanisms are mostly unknown, and a suitable animal model with internal increased ACR does not exist for in vivo studying so far. Several enzyme systems are responsible for acrolein detoxification, such as Aldehyde Dehydrogenase (ALDH), Aldo‐Keto Reductase (AKR), and Glutathione S‐Transferase (GST). To evaluate the function of ACR in glucose homeostasis and diabetes, akr1a1a−/− zebrafish mutants are generated using CRISPR/Cas9 technology. Accumulated endogenous acrolein is confirmed in akr1a1a−/− larvae and livers of adults. Moreover, a series of experiments are performed regarding organic alterations, the glucose homeostasis, transcriptome, and metabolomics in Tg(fli1:EGFP) zebrafish. Akr1a1a−/− larvae display impaired glucose homeostasis and angiogenic retina hyaloid vasculature, which are caused by reduced acrolein detoxification ability and increased internal ACR concentration. The effects of acrolein on hyaloid vasculature can be reversed by acrolein‐scavenger l‐carnosine treatment. In adult akr1a1a−/− mutants, impaired glucose tolerance accompanied by angiogenic retina vessels and glomerular basement membrane thickening, consistent with an early pathological appearance in diabetic retinopathy and nephropathy, are observed. Thus, the data strongly suggest impaired ACR detoxification and elevated ACR concentration as biomarkers and inducers for diabetes and diabetic complications