Transforming Growth Factor-Beta: Selective Increase in Glycosaminoglycan Synthesis by Cultures of Fibroblasts From Patients With Progressive Systemic Sclerosis

Transforming growth factor-beta (TGF-beta) has been found in all cells examined thus far, and has been shown to play an important role in inflammation and connective tissue formation. We now report that TGF-beta, alone or in combination with epidermal growth factor (EGF), led to a preferential increase in glycosaminoglycan synthesis by cultures of dermal fibroblasts from patients with progressive systemic sclerosis (PSS) when compared with normal fibroblasts (p < 0.001). Transforming growth factor-beta increased collagen synthesis to the same extent in both PSS and normal fibroblasts, whereas EGF had no stimulatory activity on collagen synthesis. The addition of EGE to cultures incubated with TGF-beta led to a decrease in collagen synthesis compared with the effect seen with TGF-beta alone (p <0.02). These studies suggest that TGF-beta may play an important role in the accumulation of connective tissue seen in PSS and that the combined action of multiple growth factors may modulate the synthetic activity of human dermal fibroblasts

Lymphocyte Adhesion to Psoriatic Dermal Endothelium: Mechanism and Modulation

Psoriasis is characterized by the hyperproliferation of keratinocytes in the epidermis and the accumulation of activated CD4+ T lymphocytes in the upper dermis. We have recently rested the hypothesis that the abnormal endothelial proliferation in the dermal papillae of psoriaric lesions may be mechanistically linked to the expression of endothelial ligands capable of promoting lymphocytes binding and extravasation. The results indicated that specialized endothelial cells lining the post-capillary venules of psoriatic lesions are capable of promoting the selective adherence of human CD4+ T cells and its memory subset. In contrast, B cells, CD8+ T cells, and CD45RA+ T cells are deficient in their capacities to bind. The adhesion process is energy and calcium dependent and involves tissue-specific lymphocyte receptors, with LFA-1 molecules playing an accessory role. We concluded that transformation of the dermal endothelium into a lymphocyte-receptive phenotype by defined growth factors or cytokines may represent a positive feedback mechanism promoting lymphocyte migration into the diseased sites

Estimating the Parameters of Sgr A*'s Accretion Flow Via Millimeter VLBI

Recent millimeter-VLBI observations of Sagittarius A* (Sgr A*) have, for the first time, directly probed distances comparable to the horizon scale of a black hole. This provides unprecedented access to the environment immediately around the horizon of an accreting black hole. We leverage both existing spectral and polarization measurements and our present understanding of accretion theory to produce a suite of generic radiatively inefficient accretion flow (RIAF) models of Sgr A*, which we then fit to these recent millimeter-VLBI observations. We find that if the accretion flow onto Sgr A* is well described by a RIAF model, the orientation and magnitude of the black hole's spin is constrained to a two-dimensional surface in the spin, inclination, position angle parameter space. For each of these we find the likeliest values and their 1-sigma & 2-sigma errors to be a=0(+0.4+0.7), inclination=50(+10+30)(-10-10) degrees, and position angle=-20(+31+107)(-16-29) degrees, when the resulting probability distribution is marginalized over the others. The most probable combination is a=0(+0.2+0.4), inclination=90(-40-50) degrees and position angle=-14(+7+11)(-7-11) degrees, though the uncertainties on these are very strongly correlated, and high probability configurations exist for a variety of inclination angles above 30 degrees and spins below 0.99. Nevertheless, this demonstrates the ability millimeter-VLBI observations, even with only a few stations, to significantly constrain the properties of Sgr A*.Comment: 10 pages, 7 figures, accepted by Ap

On the evolution of the Gamma- and X-ray luminosities of Pulsar Wind Nebulae

Pulsar wind nebulae are a prominent class of very high energy (E > 0.1 TeV) Galactic sources. Their Gamma-ray spectra are interpreted as due to inverse Compton scattering of ultrarelativistic electrons on the ambient photons, whereas the X-ray spectra are due to synchrotron emission. We investigate the relation between the Gamma- and-X-ray emission and the pulsars' spin-down luminosity and characteristic age. We find that the distance-independent Gamma- to X-ray flux ratio of the nebulae is inversely proportional to the spin-down luminosity, (\propto \dot{E}^-1.9), while it appears proportional to the characteristic age, (\propto tau_c^2.2), of the parent pulsar. We interpret these results as due to the evolution of the electron energy distribution and the nebular dynamics, supporting the idea of so-called relic pulsar wind nebulae. These empirical relations provide a new tool to classify unidentified diffuse Gamma-ray sources and to estimate the spin-down luminosity and characteristic age of rotation powered pulsars with no detected pulsation from the X- and Gamma-ray properties of the associated pulsar wind nebulae. We apply these relations to predict the spin-down luminosity and characteristic age of four (so far unpulsing) candidate pulsars associated to wind nebulae.Comment: Accepted for publication in ApJ (6 pages, 2 figures

Methods for detecting flaring structures in Sagittarius A* with high frequency VLBI

The super massive black hole candidate, Sagittarius A*, exhibits variability from radio to X-ray wavelengths on time scales that correspond to < 10 Schwarzschild radii. We survey the potential of millimeter-wavelength VLBI to detect and constrain time variable structures that could give rise to such variations, focusing on a model in which an orbiting hot spot is embedded in an accretion disk. Non-imaging algorithms are developed that use interferometric closure quantities to test for periodicity, and applied to an ensemble of hot-spot models that sample a range of parameter space. We find that structural periodicity in a wide range of cases can be detected on most potential VLBI arrays using modern VLBI instrumentation. Future enhancements of mm/sub-mm VLBI arrays including phased array processors to aggregate VLBI station collecting area, increased bandwidth recording, and addition of new VLBI sites all significantly aid periodicity detection. The methods described herein can be applied to other models of Sagittarius A*, including jet outflows and Magneto-Hydrodynamic accretion simulations.Comment: Submitted to Ap

GYOTO: a new general relativistic ray-tracing code

GYOTO, a general relativistic ray-tracing code, is presented. It aims at computing images of astronomical bodies in the vicinity of compact objects, as well as trajectories of massive bodies in relativistic environments. This code is capable of integrating the null and timelike geodesic equations not only in the Kerr metric, but also in any metric computed numerically within the 3+1 formalism of general relativity. Simulated images and spectra have been computed for a variety of astronomical targets, such as a moving star or a toroidal accretion structure. The underlying code is open source and freely available. It is user-friendly, quickly handled and very modular so that extensions are easy to integrate. Custom analytical metrics and astronomical targets can be implemented in C++ plug-in extensions independent from the main code.Comment: 20 pages, 11 figure

Simultaneous observations of PKS 2155-304 with H.E.S.S., Fermi, RXTE and ATOM: spectral energy distributions and variability in a low state

We report on the first simultaneous observations that cover the optical, X-ray, and high energy gamma-ray bands of the BL Lac object PKS 2155-304. The gamma-ray bands were observed for 11 days, between 25 August and 6 September 2008, jointly with the Fermi Gamma-ray Space Telescope and the H.E.S.S. atmospheric Cherenkov array, providing the first simultaneous MeV-TeV spectral energy distribution with the new generation of gamma-ray telescopes. The ATOM telescope and the RXTE and Swift observatories provided optical and X-ray coverage of the low-energy component over the same time period. The object was close to the lowest archival X-ray and Very High Energy state, whereas the optical flux was much higher. The light curves show relatively little (~30%$) variability overall when compared to past flaring episodes, but we find a clear optical/VHE correlation and evidence for a correlation of the X-rays with the high energy spectral index. Contrary to previous observations in the flaring state, we do not find any correlation between the X-ray and VHE components. Although synchrotron self-Compton models are often invoked to explain the SEDs of BL Lac objects, the most common versions of these models are at odds with the correlated variability we find in the various bands for PKS 2155-304.Comment: Accepted for publication in the Astrophysical Journa

The LOFT mission concept: a status update

The Large Observatory For x-ray Timing (LOFT) is a mission concept which was proposed to ESA as M3 and M4 candidate in the framework of the Cosmic Vision 2015-2025 program. Thanks to the unprecedented combination of effective area and spectral resolution of its main instrument and the uniquely large field of view of its wide field monitor, LOFT will be able to study the behaviour of matter in extreme conditions such as the strong gravitational field in the innermost regions close to black holes and neutron stars and the supra-nuclear densities in the interiors of neutron stars. The science payload is based on a Large Area Detector (LAD, >8m2 effective area, 2-30 keV, 240 eV spectral resolution, 1 degree collimated field of view) and a Wide Field Monitor (WFM, 2-50 keV, 4 steradian field of view, 1 arcmin source location accuracy, 300 eV spectral resolution). The WFM is equipped with an on-board system for bright events (e.g., GRB) localization. The trigger time and position of these events are broadcast to the ground within 30 s from discovery. In this paper we present the current technical and programmatic status of the mission