Evaluation of In-Vitro Antioxidant Activity of Biochanin A

This study was aimed to determine the in vitro antioxidant potential of biochanin A (BCA), an isoflavone phytochemical, by using various free radical scavenging assays. The free radical scavenging activity of BCA was evaluated by various standardized assays such as 1, 1-Diphenyl-2-Picryl Hydrazyl (DPPH●), 2, 2’-Azinobis-3-Ethylbenzothiazoline-6-Sulfonic Acid (ABTS●+), Ferric Reducing Antioxidant Power (FRAP),nitric oxide scavenging activity, reducing ability, hydroxy radical activity, superoxide anion scavenging activity, hydrogen peroxide radical, metal ion chelating activity  and phosphomolybdenum  assay. Four different concentrations of BCA (5, 10, 20, 40, μg/ml) were taken for evaluating the scavenging activity and which were compared with the antioxidant activity of standard ascorbic acid (AA). BCA showed good free radical scavenging activity, which was calculated as IC50. IC50 value of BCA was also comparable to Ascorbic acid (AA).Whereas AA was used as a standard. The scavenging activity of BCA was significantly elevated in a dose dependent manner. The BCA was exhibited a highest scavenging activity than the standard. The results obtained in the present study revealed that the BCA is an excellent free radical scavenger with the activity similar to that of AA. Keywords: Biochanin A, Antioxidant, Free radicals, Nitric oxides and Ascorbic aci

Biochanin A prevents buccal pouch carcinogenesis by enhancing carcinogen detoxification and antioxidant status in hamsters

Objective: This study objective was to ascertain the drug biochanin A (BCA) impacts on the management of oral squamous cell carcinoma (OSCC) caused by the chemical 7,12-dimethylbenz[a]anthracene (DMBA) in male Syrian golden hamsters. Methods: The oral cancer was induced by the application of a DMBA (0.5 %) thrice a week for the duration of fourteen weeks in the buccal pouch. The study assessed various parameters, including volume, incidence, and burden of tumors within the hamsters' buccal pouches. Additionally, it employed colorimetric methods to measure the levels of carcinogen-detoxifying agents, lipid peroxidation, and antioxidant activity. The histopathological examination was assessed in the buccal tissues, as well. Results: The hamsters exposed to DMBA exhibited the growth of tumors throughout the buccal pouches, accompanied by notable changes in lipid peroxidation, antioxidant levels, and carcinogen-detoxifying agents. However, oral administration of BCA (20 mg/kg bw) every second day for fourteen weeks showed significant alteration in the histopathological changes and reduced incidence, volume, and burden of tumors in DMBA-exposed hamsters. BCA established substantial anti-lipid peroxidation effects and the ability to enhance antioxidant and carcinogen-detoxifying agent levels in DMBA-exposed animals. Conclusion: The outcomes of this investigation emphasize the chemopreventive and antioxidant capability of BCA against DMBA-induced oral carcinoma in hamsters