11 research outputs found

    Analysis of Single Cells by Capillary Electrophoresis with On-Column Derivatization and Laser-Induced Fluorescence Detection

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    On-column derivatization of single mammalian cells with capillary electrophoretic separation and laser-induced fluorescence detection is described. Individual cells are electrophorectically injected into the front end of the separation capillary, which is used as a chamber to lyse the cell and derivatize its contents for subsequent separation and detection. Reagents to lyse the cell and derivatize its contents are electrophoretically injected into the front end of the capillary (2.7 mm, 600-pL volume for a 17-µm -i.d. capillary) after the cell has been injected. Dopamine and five amino acids have been quantitatively determined in individual rat pheochromocytoma cells after on-column derivatization with naphthalene-2, 3-dicarboxaldehyde and CN-. Average values of compounds determined in these cells range from 180 ±110 amol/ cell for aspartic acid to 5.1 ± 1.5 fmol/cell for taurine. © 1995, American Chemical Society. All rights reserved

    Research and Science Today Supplement 1/2014

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    Search for Transient Gravitational-wave Signals Associated with Magnetar Bursts during Advanced LIGO's Second Observing Run

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    We present the results of a search for short- and intermediate-duration gravitational-wave signals from four magnetar bursts in Advanced LIGO' s second observing run. We find no evidence of a signal and set upper bounds on the root sum squared of the total dimensionless strain (h(rss)) from incoming intermediate-duration gravitational waves ranging from 1.1 x 10(-22) at 150 Hz to 4.4 x 10(-22) at 1550 Hz at 50% detection efficiency. From the known distance to the magnetar SGR 1806-20 (8.7 kpc), we can place upper bounds on the isotropic gravitational-wave energy of 3.4 x 10(44) erg at 150 Hz assuming optimal orientation. This represents an improvement of about a factor of 10 in strain sensitivity from the previous search for such signals, conducted during initial LIGO' s sixth science run. The short-duration search yielded upper limits of 2.1 x 10(44) erg for short white noise bursts, and 2.3 x 10(47) erg for 100 ms long ringdowns at 1500 Hz, both at 50% detection efficiency
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