Distributed Access Control with Blockchain

The specification and enforcement of network-wide policies in a single administrative domain is common in today's networks and considered as already resolved. However, this is not the case for multi-administrative domains, e.g. among different enterprises. In such situation, new problems arise that challenge classical solutions such as PKIs, which suffer from scalability and granularity concerns. In this paper, we present an extension to Group-Based Policy -- a widely used network policy language -- for the aforementioned scenario. To do so, we take advantage of a permissioned blockchain implementation (Hyperledger Fabric) to distribute access control policies in a secure and auditable manner, preserving at the same time the independence of each organization. Network administrators specify polices that are rendered into blockchain transactions. A LISP control plane (RFC 6830) allows routers performing the access control to query the blockchain for authorizations. We have implemented an end-to-end experimental prototype and evaluated it in terms of scalability and network latency.Comment: 7 pages, 9 figures, 2 table

The necropolis of Can Gambus-1 (Sabadell, Barcelona). New data on the funerary practices during the Middle Neolithic in the northeast of the Iberian Peninsula

In this paper we deal with the Neolithic burials at the site of Can Gambus-1, belonging of the so called Cultura de los Sepulcros de Fosa" (from end of the fifth to the beginning of the fourth millennia cal BC). The main aim is to present new data on the procedures of excavation of the pits, the materials used in the covering of the graves and the treatment given to some of the corpses. The detailed archaeological reconstruction of the funerary behaviour has been possible thanks to the exceptional state of preservation of the graves, the rigorous process of archaeological excavation, the anthropological study carried out, as well as to the achievement of a detailed taxonomic study of all the available evidences. Likewise, the grave goods recovered in the burials are presented. Their quantity and quality is outstanding compared with other similar funerary contexts. Probably this fact is due to the exclusive presence of adult individuals among the buried ones

Role of flow cytometry immunophenotyping in the diagnosis of leptomeningeal carcinomatosis

PURPOSE: To explore the contribution of flow cytometry immunophenotyping (FCI) in detecting leptomeningeal disease in patients with solid tumors. EXPERIMENTAL DESIGN: Cerebrospinal fluid (CSF) samples from 78 patients who received a diagnosis of epithelial-cell solid tumors and had clinical data suggestive of leptomeningeal carcinomatosis (LC) were studied. A novel FCI protocol was used to identify cells expressing the epithelial cell antigen EpCAM and their DNA content. Accompanying inflammatory cells were also described. FCI results (positive or negative for malignancy) were compared with those from CSF cytology and with the diagnosis established by the clinicians: patients with LC (n = 49), without LC (n = 26), and undetermined (n = 3). RESULTS: FCI described a wide range of EpCAM-positive cells with a hyperdiploid DNA content in the CSF of patients with LC. Compared with cytology, FCI showed higher sensitivity (75.5 vs 65.3) and negative predictive value (67.6 vs 60.5), and similar specificity (96.1 vs 100) and positive predictive value (97.4 vs 100). Concordance between cytology and FCI was high (Kp = 0.83), although misdiagnosis of LC did not show differences between evaluating the CSF with 1 or 2 techniques (P = .06). Receiver-operator characteristic curve analyses showed that lymphocytes and monocytes had a different distribution between patients with and without LC. CONCLUSION: FCI seems to be a promising new tool for improving the diagnostic examination of patients with suspicion of LC. Detection of epithelial cells with a higher DNA content is highly specific of LC, but evaluation of the nonepithelial cell compartment of the CSF might also be useful for supporting this diagnosis

Role of flow cytometry immunophenotyping in the diagnosis of leptomeningeal carcinomatosis

PURPOSE: To explore the contribution of flow cytometry immunophenotyping (FCI) in detecting leptomeningeal disease in patients with solid tumors. EXPERIMENTAL DESIGN: Cerebrospinal fluid (CSF) samples from 78 patients who received a diagnosis of epithelial-cell solid tumors and had clinical data suggestive of leptomeningeal carcinomatosis (LC) were studied. A novel FCI protocol was used to identify cells expressing the epithelial cell antigen EpCAM and their DNA content. Accompanying inflammatory cells were also described. FCI results (positive or negative for malignancy) were compared with those from CSF cytology and with the diagnosis established by the clinicians: patients with LC (n = 49), without LC (n = 26), and undetermined (n = 3). RESULTS: FCI described a wide range of EpCAM-positive cells with a hyperdiploid DNA content in the CSF of patients with LC. Compared with cytology, FCI showed higher sensitivity (75.5 vs 65.3) and negative predictive value (67.6 vs 60.5), and similar specificity (96.1 vs 100) and positive predictive value (97.4 vs 100). Concordance between cytology and FCI was high (Kp = 0.83), although misdiagnosis of LC did not show differences between evaluating the CSF with 1 or 2 techniques (P = .06). Receiver-operator characteristic curve analyses showed that lymphocytes and monocytes had a different distribution between patients with and without LC. CONCLUSION: FCI seems to be a promising new tool for improving the diagnostic examination of patients with suspicion of LC. Detection of epithelial cells with a higher DNA content is highly specific of LC, but evaluation of the nonepithelial cell compartment of the CSF might also be useful for supporting this diagnosis

Structural and functional evaluation of the palindromic alanine-rich antimicrobial peptide Pa-MAP2

© 2016 Published by Elsevier B.V.Recently, several peptides have been studied regarding the defence process against pathogenic microorganisms, which are able to act against different targets, with the purpose of developing novel bioactive compounds. The present work focuses on the structural and functional evaluation of the palindromic antimicrobial peptide Pa-MAP2, designed based on the peptide Pa-MAP from Pleuronectes americanus. For a better structural understanding, molecular modelling analyses were carried out, together with molecular dynamics and circular dichroism, in different media. Antibacterial activity against Gram-negative and positive bacteria was evaluated, as well as cytotoxicity against human erythrocytes, RAW 264.7, Vero and L6 cells. In silico docking experiments, lipid vesicle studies, and atomic force microscopy (AFM) imaging were carried out to explore the activity of the peptide. In vivo studies on infected mice were also done. The palindromic primary sequence favoured an α-helix structure that was pH dependent, only present on alkaline environment, with dynamic N- and C-terminals that are stabilized in anionic media. Pa-MAP2 only showed activity against Gram-negative bacteria, with a MIC of 3.2 μM, and without any cytotoxic effect. In silico, lipid vesicles and AFM studies confirm the preference for anionic lipids (POPG, POPS, DPPE, DPPG and LPS), with the positively charged lysine residues being essential for the initial electrostatic interaction. In vivo studies showed that Pa-MAP2 increases to 100% the survival rate of mice infected with Escherichia coli. Data here reported indicated that palindromic Pa-MAP2 could be an alternative candidate for use in therapeutics against Gram-negative bacterial infections.This work was supported by the Brazilian funding agencies CNPq, CAPES, FADPDF, FINEP and FUNDECT, by Fundação para a Ciência e a Tecnologia — Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal), and by Marie Skłodowska-Curie Research and Innovation Staff Exchange (MSCA-RISE, European Union) project INPACT (call H2020-MSCA-RISE-2014, grant agreement 644167). MRF also acknowledges FCT-MCTES fellowship SPRH/BD/100517/2014.info:eu-repo/semantics/publishedVersio