Erythrocyte enrichment in hematopoietic progenitor cell cultures based on magnetic susceptibility of the hemoglobin

Using novel media formulations, it has been demonstrated that human placenta and umbilical cord blood-derived CD34+ cells can be expanded and differentiated into erythroid cells with high efficiency. However, obtaining mature and functional erythrocytes from the immature cell cultures with high purity and in an efficient manner remains a significant challenge. A distinguishing feature of a reticulocyte and maturing erythrocyte is the increasing concentration of hemoglobin and decreasing cell volume that results in increased cell magnetophoretic mobility (MM) when exposed to high magnetic fields and gradients, under anoxic conditions. Taking advantage of these initial observations, we studied a noninvasive (label-free) magnetic separation and analysis process to enrich and identify cultured functional erythrocytes. In addition to the magnetic cell separation and cell motion analysis in the magnetic field, the cell cultures were characterized for cell sedimentation rate, cell volume distributions using differential interference microscopy, immunophenotyping (glycophorin A), hemoglobin concentration and shear-induced deformability (elongation index, EI, by ektacytometry) to test for mature erythrocyte attributes. A commercial, packed column high-gradient magnetic separator (HGMS) was used for magnetic separation. The magnetically enriched fraction comprised 80% of the maturing cells (predominantly reticulocytes) that showed near 70% overlap of EI with the reference cord blood-derived RBC and over 50% overlap with the adult donor RBCs. The results demonstrate feasibility of label-free magnetic enrichment of erythrocyte fraction of CD34+ progenitor-derived cultures based on the presence of paramagnetic hemoglobin in the maturing erythrocytes. © 2012 Jin et al

Normal modes for metric fluctuations in a class of higher-dimensional backgrounds

We discuss a gauge invariant approach to the theory of cosmological perturbations in a higher-dimensonal background. We find the normal modes which diagonalize the perturbed action, for a scalar field minimally coupled to gravity, in a higher-dimensional manifold M of the Bianchi-type I, under the assumption that the translations along an isotropic spatial subsection of M are isometries of the full, perturbed background. We show that, in the absence of scalar field potential, the canonical variables for scalar and tensor metric perturbations satisfy exactly the same evolution equation, and we discuss the possible dependence of the spectrum on the number of internal dimensions.Comment: 19 pages, LATEX, an explicit example is added to discuss the possible dependence of the perturbation spectrum on the number of internal dimensions. To apper in Class. Quantum Gra

Discriminating between the origins of remotely sensed circular structures:carbonate mounds, diapirs or periclinal folds? Purbeck Limestone Group, Weymouth Bay, UK

Many sedimentary rock successions contain plan-view circular structures, such as impacts, diapirs and carbonate build-ups. When remotely sensed, it can be difficult to discriminate between their formation mechanisms. Here we examine this problem by assessing the origins of circular structures imaged in high-resolution multibeam bathymetric data from Weymouth Bay, UK. The imagery shows 30–150 m across, concave-down structures within the upper Purbeck Limestone Group on the southern limb of the Purbeck Anticline. Similar structures have not been identified in the extensive outcrops around the bay. The morphology and geological setting of the structures are consistent with three different interpretations: carbonate mounds, periclinal folds and evaporite diapirs. However, none of these structures has been previously recorded in the upper Purbeck Limestone Group outcrops of this internationally renowned geological region. We apply a scoring system to 25 features of the circular structures to discriminate between these three alternative interpretations. This analysis indicates that evaporite diapirs are the least likely and carbonate mounds the most likely origin of the structures. The presence of carbonate mounds revises the upper Purbeck palaeofacies distribution in its type area and provides an analogue for the exploration for hydrocarbon reservoirs in lacustrine mounds

The Pre-Big Bang Scenario in String Cosmology

We review physical motivations, phenomenological consequences, and open problems of the so-called pre-big bang scenario in superstring cosmology.Comment: 250 pages, latex, 34 figures included using epsfi