Peran Corporate Entrepreneurship pada Persaingan dan Keberlangsungan Bisnis CV. Alam Mulia

Kegiatan praktik kerja magang dilakukan di perusahaan CV.Alam Mulia pada divisi Marketing Communication selama 3 bulan. CV.Alam Mulia merupakan perusahaan penyedia jasa pelayanan pernikahan yang berfokus untuk melayani di wilayah jabodetabek dan jawabarat. Selama melakukan praktik kerja magang, penulis berfokus untuk melihat bagaimana implementasi teori corporate entrepreneurship yang dilakukan dalam perusahaan ini, dengan melalui job description yang diberikan selama periode magang, penulis mendapatkan beberapa implementasi corporate entrepreneurship yang sudah dilakukan perusahaan, dengan mengetahui hal tersebut diharapkan penulis dapat memberi masukan untuk perusahaan agar dapat bertahan terutama di masa pandemi covid-19 saat ini. Adapun tujuan dari praktik kerja magang ini adalah agar mendapatkan pengalaman dan pemahaman secara langsung terkait teori yang penulis pilih yang telah diajarkan pada perkuliahan

Analisis Pengaruh Prosocial Motivation yang dimediasi oleh Creativity in Social Work terhadap Social Entrepreneurial Intention

Penelitian ini bertitik tolak dari maraknya permasalahan sosial di Indonesia yang tidak kunjung terselesaikan, mulai dari isu lingkungan, tingginya angka kemiskinan dan pengangguran yang disebabkan karena kualitas pendidikan di Indonesia yang tidak merata sehingga sebagian masyarakat tidak mampu bersaing dalam pekerjaan. Faktor lain juga disebabkan dari kurangnya minat masyarakat dalam membangun wirausaha sendiri dan lebih memilih untuk berkarir menjadi karyawan swasta atau PNS setelah lulus dari bangku sekolah. Dalam hal ini keberadaan social entrepreneurship diharapkan dapat berkontribusi penuh dalam mengurangi permasalahan sosial di Indonesia. Penelitian ini bertujuan untuk menganalisis seberapa besar minat mahasiswa dalam membangun bisnis sosial dengan mempertimbangkan faktor prosocial motivation dan creativity in social work. Metode yang digunakan pada penelitian ini adalah deskriptif kuantitatif melalui penyebaran kuesioner online kepada sejumlah 158 responden mahasiswa Universitas Pendidikan Indonesia dengan kriteria yang sudah pernah melakukan pengabdian sosial dan belum memiliki bisnis, serta teknik pengambilan sampel yang digunakan adalah non-probability sampling jenis purposive sampling. Pengolahan data menggunakan metode PLS-SEM dengan software SmartPLS 3.0 untuk menguji bagaimana hubungan konstruk dengan melihat apakah terdapat atau tidak adanya pengaruh antar konstruk yang diteliti. Hasil penelitian menunjukan bahwa prosocial motivation dan creativity in social work berpengaruh positif dan signifikan terhadap social entrepreneurial intention, dan creativity in social work berpengaruh memediasi hubungan antara prosocial motivation dan social entrepreneurial intention. Sehingga dapat disimpulkan bahwa creativity in social work berperan memediasi parsial hubungan antara prosocial motivation dengan social entrepreneurial intention

PROFIL PASTING DAN MUTU FISIK TEPUNG KACANG BAMBARA BOGOR BERDASARKAN WARNA KULIT ARI

Kacang Bambara varietas Bogor merupakan tanaman lokal berasal dari Afrika yang memiliki variasi warna kulit dan dapat diolah menjadi tepung. Tujuan penelitian ini adalah mempelajari pengaruh warna kulit ari terhadap profil pasting dan mutu fisik tepung kacang Bambara Bogor serta potensi pemanfaatannya. Penelitian meliputi pembuatan tepung kacang Bambara Bogor dengan kulit ari berwarna putih, ungu muda, ungu tua, dan hitam. Rancangan penelitian menggunakan rancangan acak lengkap 1 faktor dengan dua ulangan. Analisis yang dilakukan meliputi profil pasting yang terdiri dari suhu pasting, viskositas puncak, waktu puncak, viskositas breakdown, dan setback viscosity. Analisis fisik meliputi rendemen, tingkat kecerahan, daya serap air dari tepung kacang Bambara Bogor. Hasil penelitian menunjukkan bahwa warna kulit ari yang semakin gelap mengakibatkan penurunan pada parameter suhu pasting, viskositas puncak, breakdown, dan setback viscosity, sedangkan waktu puncak cenderung meningkat. Selain itu, warna kulit ari yang semakin gelap menyebabkan tingkat kecerahan semakin meningkat, sedangkan rendemen dan daya serap air semakin menurun. Berdasarkan profil pasting dan daya serap air, tepung kacang Bambara Bogor cocok untuk cookies, produk rehidrasi, dan produk yang membutuhkan kestabilan pasta pada suhu rendah, seperti saos dan pudding. Kata kunci : Vigna subterranea (L.) Verdcourt, tepung kacang bogor, variasi warna epidermis, gelatinisasi, mutu fisi

Swarm Micro UAVs for Area Mapping in GPS-denied Areas

Using micro–Unmanned Aerial Vehicles (UAVs) or Micro Aerial Vehicles (MAVs) for mapping and cartography applications has the potential to change the way UAVs and MAVs are used, especially in treacherous and GPS-denied locations. Mapping in the past would take place with a person using various tools to map or survey any given area, but there has been a fundamental switch to autonomous vehicles that can accomplish the task in less time and with less effort. Autonomous mapping practices are typically completed using an individual UAV that maps using Simultaneous Localization and Mapping (SLAM) or photogrammetry. This project aims to use swarm robotics to map complex environments and harsh terrain using MAVs with a quicker, more accurate, and more precise method. The scope of the mapping procedure will cover difficult-to-reach locations such as cliffs, abandoned buildings, and forests. It also covers areas that would take too long for a surveyor to do by hand, such as construction sites and large indoor spaces like warehouses, factories, or historical buildings that cannot be modified for surveying. The swarm will utilize an emergent-like behavior to map in any given location without collisions of MAVs or ground objects

53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination.

53BP1 activity drives genome instability and lethality in BRCA1-deficient mice by inhibiting homologous recombination (HR). The anti-recombinogenic functions of 53BP1 require phosphorylation-dependent interactions with PTIP and RIF1/shieldin effector complexes. While RIF1/shieldin blocks 5'-3' nucleolytic processing of DNA ends, it remains unclear how PTIP antagonizes HR. Here, we show that mutation of the PTIP interaction site in 53BP1 (S25A) allows sufficient DNA2-dependent end resection to rescue the lethality of BRCA1Δ11 mice, despite increasing RIF1 "end-blocking" at DNA damage sites. However, double-mutant cells fail to complete HR, as excessive shieldin activity also inhibits RNF168-mediated loading of PALB2/RAD51. As a result, BRCA1Δ1153BP1S25A mice exhibit hallmark features of HR insufficiency, including premature aging and hypersensitivity to PARPi. Disruption of shieldin or forced targeting of PALB2 to ssDNA in BRCA1D1153BP1S25A cells restores RNF168 recruitment, RAD51 nucleofilament formation, and PARPi resistance. Our study therefore reveals a critical function of shieldin post-resection that limits the loading of RAD51.We thank Anthony Tubbs for comments on the paper; Jennifer Mehalko and Dom Esposito (Protein Expression Laboratory, Frederick National Laboratory for Cancer Research) for transgenic constructs; Karim Baktiar, Diana Haines, and Elijah Edmonson (Pathology/Histotechnology Laboratory, Frederick National Laboratory for Cancer Research) for rodent necropsy, pathology analysis, and imaging; Joseph Kalen and Nimit Patel (Small Animal Imaging Program, Frederick National Laboratory for Cancer Research) for X-ray computed tomography (CT) scan imaging; Jennifer Wise and Kelly Smith for assistance with animal work; Davide Robbiani and Kai Ge for antibodies; Dan Durocher for shieldin constructs; David Goldstein and the CCR Genomics core for sequencing support; and Neil Johnson for discussions. Research in the J.M.S. laboratory is supported by NIH grant R01CA197506. Research in the N.M. laboratory is supported by NIH grant R01 227001. The A.N. laboratory is supported by the Intramural Research Program of the NIH, an Ellison Medical Foundation Senior Scholar in Aging Award (AG-SS-2633-11), the Department of Defense Idea Expansion (W81XWH-15-2-006) and Breakthrough (W81XWH-16-1-599) Awards, the Alex's Lemonade Stand Foundation Award, and an NIH Intramural FLEX Award.S

Epigenomic modifications mediating antibody maturation

Epigenetic modifications, such as histone modifications, DNA methylation status, and non-coding RNAs (ncRNA), all contribute to antibody maturation during somatic hypermutation (SHM) and class-switch recombination (CSR). Histone modifications alter the chromatin landscape and, together with DNA primary and tertiary structures, they help recruit Activation-Induced Cytidine Deaminase (AID) to the immunoglobulin (Ig) locus. AID is a potent DNA mutator, which catalyzes cytosine-to-uracil deamination on single-stranded DNA to create U:G mismatches. It has been shown that alternate chromatin modifications, in concert with ncRNAs and potentially DNA methylation, regulate AID recruitment and stabilize DNA repair factors. We, hereby, assess the combination of these distinct modifications and discuss how they contribute to initiating differential DNA repair pathways at the Ig locus, which ultimately leads to enhanced antibody-antigen binding affinity (SHM) or antibody isotype switching (CSR). We will also highlight how misregulation of epigenomic regulation during DNA repair can compromise antibody development and lead to a number of immunological syndromes and cancer

A Role for FACT in RNA Polymerase II Promoter-Proximal Pausing

FACT (facilitates chromatin transcription) is an evolutionarily conserved histone chaperone that was initially identified as an activity capable of promoting RNA polymerase II (Pol II) transcription through nucleosomes in vitro. In this report, we describe a global analysis of FACT function in Pol II transcription in Drosophila. We present evidence that loss of FACT has a dramatic impact on Pol II elongation-coupled processes including histone H3 lysine 4 (H3K4) and H3K36 methylation, consistent with a role for FACT in coordinating histone modification and chromatin architecture during Pol II transcription. Importantly, we identify a role for FACT in the maintenance of promoter-proximal Pol II pausing, a key step in transcription activation in higher eukaryotes. These findings bring to light a broader role for FACT in the regulation of Pol II transcription

The DSIF Subunits Spt4 and Spt5 Have Distinct Roles at Various Phases of Immunoglobulin Class Switch Recombination

Class-switch recombination (CSR), induced by activation-induced cytidine deaminase (AID), can be divided into two phases: DNA cleavage of the switch (S) regions and the joining of the cleaved ends of the different S regions. Here, we show that the DSIF complex (Spt4 and Spt5), a transcription elongation factor, is required for CSR in a switch-proficient B cell line CH12F3-2A cells, and Spt4 and Spt5 carry out independent functions in CSR. While neither Spt4 nor Spt5 is required for transcription of S regions and AID, expression array analysis suggests that Spt4 and Spt5 regulate a distinct subset of transcripts in CH12F3-2A cells. Curiously, Spt4 is critically important in suppressing cryptic transcription initiating from the intronic Sμ region. Depletion of Spt5 reduced the H3K4me3 level and DNA cleavage at the Sα region, whereas Spt4 knockdown did not perturb the H3K4me3 status and S region cleavage. H3K4me3 modification level thus correlated well with the DNA breakage efficiency. Therefore we conclude that Spt5 plays a role similar to the histone chaperone FACT complex that regulates H3K4me3 modification and DNA cleavage in CSR. Since Spt4 is not involved in the DNA cleavage step, we suspected that Spt4 might be required for DNA repair in CSR. We examined whether Spt4 or Spt5 is essential in non-homologous end joining (NHEJ) and homologous recombination (HR) as CSR utilizes general repair pathways. Both Spt4 and Spt5 are required for NHEJ and HR as determined by assay systems using synthetic repair substrates that are actively transcribed even in the absence of Spt4 and Spt5. Taken together, Spt4 and Spt5 can function independently in multiple transcription-coupled steps of CSR

Polo kinase recruitment via the constitutive centromere-associated network at the kinetochore elevates centromeric RNA

The kinetochore, a multi-protein complex assembled on centromeres, is essential to segregate chromosomes during cell division. Deficiencies in kinetochore function can lead to chromosomal instability and aneuploidy-a hallmark of cancer cells. Kinetochore function is controlled by recruitment of regulatory proteins, many of which have been documented, however their function often remains uncharacterized and many are yet to be identified. To identify candidates of kinetochore regulation we used a proteome-wide protein association strategy in budding yeast and detected many proteins that are involved in post-translational modifications such as kinases, phosphatases and histone modifiers. We focused on the Polo-like kinase, Cdc5, and interrogated which cellular components were sensitive to constitutive Cdc5 localization. The kinetochore is particularly sensitive to constitutive Cdc5 kinase activity. Targeting Cdc5 to different kinetochore subcomplexes produced diverse phenotypes, consistent with multiple distinct functions at the kinetochore. We show that targeting Cdc5 to the inner kinetochore, the constitutive centromere-associated network (CCAN), increases the levels of centromeric RNA via an SPT4 dependent mechanism