Mid-infrared observations of the transitional disks around DH Tau, DM Tau, and GM Aur

Aims: We present mid-infrared observations and photometry of the transitional disks around the young stellar objects DH Tau, DM Tau, and GM Aur, obtained with VISIR/VLT in N band. Our aim is to resolve the inner region and the large-scale structures of these transitional disks, carrying potential signatures of intermediate or later stages of disk evolution and ongoing planet formation. Methods: We use the simultaneously observed standard-stars as PSF reference to constrain the radial flux profiles of our target objects. Subtracting the obtained standard-star profile from the corresponding science object profile yields the flux residuals produced by the star-disk system. A detection threshold takes into account the background standard deviation and also the seeing variations during the observations to evaluate the significance of these flux residuals. On the basis of a simple model for the dust re-emission, we derive constraints on the inner radius of the dust disk. Results: We spatially resolve the transitional disk around GM Aur and determine an inner-disk hole radius of 20.5(+1.0,-0.5) AU. The circumstellar disks around DH Tau and DM Tau are not spatially resolved but we are able to constrain the inner-disk hole radius to <15.5(+9.0,-2.0) AU and <15.5(+0.5,-0.5) AU, respectively. The performed photometry yields fluxes of 178+-31 mJy for DH Tau, 56+-6 mJy for DM Tau, and 229+-14 mJy for GM Aur.Comment: Accepted for publication in Astronomy & Astrophysics. (6 pages, including 7 figures and 5 tables

Substructure in the lens HE 0435-1223

We investigate the properties of dark matter substructure in the gravitational lens HE 0435-1223 (z_l=0.455) via its effects on the positions and flux ratios of the quadruply-imaged background quasar (z_s=1.689). We start with a smooth mass model, add individual, truncated isothermal clumps near the lensed images, and use the Bayesian evidence to compare the quality of different models. Compared with smooth models, models with at least one clump near image A are strongly favored. The mass of this clump within its Einstein radius is log(Mein/Msun) = 7.65 +0.87/-0.84. The Bayesian evidence provides weaker support for a second clump near image B, with log(Mein/Msun) = 6.55 +1.01/-1.51. We also examine models with a full population of substructure, and find the mass fraction in substructure at the Einstein radius to be f_sub > 0.00077, assuming the total clump masses follow a mass function dN/dM proportional to M^(-1.9) over the range M = 10^7-10^10 Msun. Few-clump and population models produce similar Bayesian evidence values, so neither type of model is objectively favored.Comment: 18 pages, 12 figures, 4 tables. MNRAS accepte

Overview on Multienzymatic Cascades for the Production of Non-canonical α-Amino Acids

SM-R thanks the University of Granada for the support provided by project PPJI2017-1 and the European Cooperation in Science and Technology (COST Action CA15133). Authors are also grateful to the Andalusian Regional Government through Endocrinology & Metabolism Group (CTS-202).The 22 genetically encoded amino acids (AAs) present in proteins (the 20 standard AAs together with selenocysteine and pyrrolysine), are commonly referred as proteinogenic AAs in the literature due to their appearance in ribosome-synthetized polypeptides. Beyond the borders of this key set of compounds, the rest of AAs are generally named imprecisely as non-proteinogenic AAs, even when they can also appear in polypeptide chains as a result of post-transductional machinery. Besides their importance as metabolites in life, many of D-α- and L-α-“non-canonical” amino acids (NcAAs) are of interest in the biotechnological and biomedical fields. They have found numerous applications in the discovery of new medicines and antibiotics, drug synthesis, cosmetic, and nutritional compounds, or in the improvement of protein and peptide pharmaceuticals. In addition to the numerous studies dealing with the asymmetric synthesis of NcAAs, many different enzymatic pathways have been reported in the literature allowing for the biosynthesis of NcAAs. Due to the huge heterogeneity of this group of molecules, this review is devoted to provide an overview on different established multienzymatic cascades for the production of non-canonical D-α- and L-α-AAs, supplying neophyte and experienced professionals in this field with different illustrative examples in the literature. Whereas the discovery of new or newly designed enzymes is of great interest, dusting off previous enzymatic methodologies by a “back and to the future” strategy might accelerate the implementation of new or improved multienzymatic cascades.University of Granada PPJI2017-1European Cooperation in Science and Technology (COST) CA15133Andalusian Regional Government through Endocrinology & Metabolism Group CTS-20

Directed Evolution of an Artificial Imine Reductase

Artificial metalloenzymes, resulting from incorporation of a metal cofactor within a host protein, have received increasing attention in the last decade. The directed evolution is presented of an artificial transfer hydrogenase (ATHase) based on the biotin-streptavidin technology using a straightforward procedure allowing screening in cell-free extracts. Two streptavidin isoforms were yielded with improved catalytic activity and selectivity for the reduction of cyclic imines. The evolved ATHases were stable under biphasic catalytic conditions. The X-ray structure analysis reveals that introducing bulky residues within the active site results in flexibility changes of the cofactor, thus increasing exposure of the metal to the protein surface and leading to a reversal of enantioselectivity. This hypothesis was confirmed by a multiscale approach based mostly on molecular dynamics and protein-ligand dockings

The ABC130 barrel module prototyping programme for the ATLAS strip tracker

For the Phase-II Upgrade of the ATLAS Detector, its Inner Detector, consisting of silicon pixel, silicon strip and transition radiation sub-detectors, will be replaced with an all new 100 % silicon tracker, composed of a pixel tracker at inner radii and a strip tracker at outer radii. The future ATLAS strip tracker will include 11,000 silicon sensor modules in the central region (barrel) and 7,000 modules in the forward region (end-caps), which are foreseen to be constructed over a period of 3.5 years. The construction of each module consists of a series of assembly and quality control steps, which were engineered to be identical for all production sites. In order to develop the tooling and procedures for assembly and testing of these modules, two series of major prototyping programs were conducted: an early program using readout chips designed using a 250 nm fabrication process (ABCN-25) and a subsequent program using a follow-up chip set made using 130 nm processing (ABC130 and HCC130 chips). This second generation of readout chips was used for an extensive prototyping program that produced around 100 barrel-type modules and contributed significantly to the development of the final module layout. This paper gives an overview of the components used in ABC130 barrel modules, their assembly procedure and findings resulting from their tests.Comment: 82 pages, 66 figure