High chromosomal instability in adenocarcinoma of the ileum arising from multifocal gastric heterotopia with gastritis cystica profunda

Adenocarcinoma of the small intestine arising from heterotopic gastric mucosa is extremely rare. In this report, we present the case of a 68-year-old woman who complained of abdominal pain, weight loss and subileus. Gross examination of resected small bowel revealed multiple flat polypous lesions with cysts in the ileal submucosa, one of which containing an ulcerated, stenosing tumour. On microscopic examination, an adenocarcinoma of the ileum arising from multifocal gastric heterotopia with secondary gastritis cystica profunda was diagnosed. Comparative genomic hybridization of the adenocarcinoma revealed chromosomal gains at 1q, 3q, 5p, 8q, 11p, 12p, 13q and losses at Xp, 4q, 8p, 10p, 14q, 17p, 20p, compatible with a high degree of genomic instability

Устройство автоматического регулирования жидкости

Although the significance of tumour site for estimating malignant potential in gastrointestinal stromal tumours (GISTs) has recently been recognized, site-specific genetic patterns have not to date been defined. This study examined 52 c-kit-positive primary GISTs (with a mean follow-up of 42.3 months in 51 cases) from three different locations (35 gastric, 12 small intestinal, and five colorectal) using comparative genomic hybridization (CGH). In general, tumour site correlated with key prognostic factors, including tumour size, mitotic rate, proliferative activity, and probable malignant potential. Furthermore, several DNA copy number changes showed a site-dependent pattern. These included losses at 14q (gastric 83%, intestinal 35%; p = 0.001), losses at 22q (gastric 46%, intestinal 82%; p = 0.02), losses at 1p (gastric 23%, intestinal 88%; p = 1 × 10-5), losses at 15q (gastric 14%, intestinal 59%; p = 0.002), losses at 9q (gastric 14%, intestinal 53%; p = 0.006), and gains at 5p (gastric 11%, intestinal 53%; p = 0.002). These data demonstrate strong site-dependent genetic heterogeneity in GISTs that may form a basis for subclassification. Prognostic evaluation of DNA copy number changes identified losses at 9q as a site-independent prognostic marker associated with shorter disease-free survival (p = 0.03) and overall survival (p = 0.002). Furthermore, 9q loss also appeared to carry prognostic value in predicting overall survival for patients with advanced or progressive GISTs (p = 0.003). Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd

Scientific drilling projects in ancient lakes: integrating geological and biological histories

Sedimentary sequences in ancient or long-lived lakes can reach several thousands of meters in thickness and often provide an unrivalled perspective of the lake's regional climatic, environmental, and biological history. Over the last few years, deep drilling projects in ancient lakes became increasingly multi- and interdisciplinary, as, among others, seismological, sedimentological, biogeochemical, climatic, environmental, paleontological, and evolutionary information can be obtained from sediment cores. However, these multi- and interdisciplinary projects pose several challenges. The scientists involved typically approach problems from different scientific perspectives and backgrounds, and setting up the program requires clear communication and the alignment of interests. One of the most challenging tasks, besides the actual drilling operation, is to link diverse datasets with varying resolution, data quality, and age uncertainties to answer interdisciplinary questions synthetically and coherently. These problems are especially relevant when secondary data, i.e., datasets obtained independently of the drilling operation, are incorporated in analyses. Nonetheless, the inclusion of secondary information, such as isotopic data from fossils found in outcrops or genetic data from extant species, may help to achieve synthetic answers. Recent technological and methodological advances in paleolimnology are likely to increase the possibilities of integrating secondary information, e.g., through molecular dating of molecular phylogenies. Some of the new approaches have started to revolutionize scientific drilling in ancient lakes, but at the same time, they also add a new layer of complexity to the generation and analysis of sediment core data. The enhanced opportunities presented by new scientific approaches to study the paleolimnological history of these lakes, therefore, come at the expense of higher logistic, communication, and analytical efforts. Here we review types of data that can be obtained in ancient lake drilling projects and the analytical approaches that can be applied to empirically and statistically link diverse datasets for creating an integrative perspective on geological and biological data. In doing so, we highlight strengths and potential weaknesses of new methods and analyses, and provide recommendations for future interdisciplinary deep drilling projects

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer‐reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state‐of‐the‐art handbook for basic and clinical researchers.DFG, 389687267, Kompartimentalisierung, Aufrechterhaltung und Reaktivierung humaner Gedächtnis-T-Lymphozyten aus Knochenmark und peripherem BlutDFG, 80750187, SFB 841: Leberentzündungen: Infektion, Immunregulation und KonsequenzenEC/H2020/800924/EU/International Cancer Research Fellowships - 2/iCARE-2DFG, 252623821, Die Rolle von follikulären T-Helferzellen in T-Helferzell-Differenzierung, Funktion und PlastizitätDFG, 390873048, EXC 2151: ImmunoSensation2 - the immune sensory syste

Colloidal properties of human transferrinreceptor

Abstract 3 1 Einleitung 5 1.1 Die zellulare Eisenaufnahme bei Vertebraten 5 1.2 Struktur und Bindungsverhalten des hTfR 7 1.3 Zielsetzung der Studie 8 2 Materialien und Methoden 11 2.1 Lichtstreuung 11 2.1.1 Die statische Lichtstreuung 12 2.1.2 Die Photonenkorrelationsspektroskopie 14 2.1.3 Experimentelle Durchführung 16 2.2 Fluoreszenz und Fluoreszenzkorrelationspektroskopie 19 2.2.1 Theorie der Fluoreszenzkorrelationsspektroskopie 21 2.2.2 Experimentelle Durchführung 23 2.3 Kalorimetrie 26 2.3.1 Differenz Scanning Kalorimetrie 27 2.3.2 Differenz-Titrations-Kalorimetrie 29 2.4 Analytische Ultrazentrifugation 31 2.4.1 Experimentelle Durchführung 32 2.5 Circulardichroismus (CD) 34 2.5.1 Experimentelle Durchführung 36 2.6 Proteinreinigung und Probenvorbereitung 37 2.6.1 Reinigung des Transferrinrezeptors 37 2.6.2 Probenvorbereitung 40 3 Ergebnisse 43 3.1 Kolloidale Eigenschaften 43 3.2 Aggregationsverhalten 46 3.3 Rezeptor-Ligand Wechselwirkungen 58 4 Diskussion 67 4.1 Kolloidale Eigenschaften 67 4.2 Aggregation 69 4.3 Rezeptor-Ligand Wechselwirkungen 78 4.4 Zusammenfassung und Ausblick 82 5 Literaturverzeichnis 85   Abbkürzungen 89   Danksagung 90   Lebenslauf 91Zusammenfassung Im Rahmen der vorliegenden Studie wurden die kolloidalen Eigenschaften des humanen Transferrinrezeptors (hTfR) in detergenzfreier Lösung mit Hilfe von Lichtstreuung und analytischer Ultrazentrifugation untersucht. Bei einer Temperatur von 293 K und pH 7,5 bildet hTfR definierte Aggregate mit einem apparenten hydrodynamischen Radius von 17 nm. Diese Aggregate bestehen aus 9 hTfR Dimeren, welche so angeordnet sind, daß eine torusförmige Struktur entsteht. Neben den Aggregaten konnten weder durch Lichtstreuung noch durch analytische Ultrazentrifugation freie Dimere nachgewiesen werden. Durch Erhöhen der Temperatur auf 340 K, Absenken des pH Wertes auf 5.0 oder N-Deglykosylierung läßt sich eine weitere Aggregation des hTfR induzieren. Diese Aggregation verläuft nach einem RLCA (reaction limited colloidal aggregation) Mechanismus. Im Verlauf der temperaturinduzierten Aggregation bilden sich mikrokristalline Strukturen. Daraus läßt sich schließen, daß neben der RLCA Aggregation auch ein Nukleationsprozess auftritt. Die Wechselwirkungen zwischen hTfR und seinem Liganden Transferrin wurden mit Hilfe der Fluoreszenzkorrelationsspektroskopie untersucht. Hierbei wurden die Bindungs- und Geschwindigkeitskonstanten der Reaktion bestimmt. Die experimentellen Befunde lassen sich mit einer Bindung von Transferrin an gleiche und unabhängige Bindungsstellen beschreiben.Abstract In the present study the colloidal properties of human Transferrinreceptor (hTfR) in detergent free solution were studied using light scattering techniques and analytical ultracentrifugation. At a temperature of 293 K and at pH 7.5, hTfR forms stable and clearly defined aggregates with an apparent hydrodynamic radius of 17 nm. Those aggregates include 9 receptor dimers, which form a toruslike structure. Receptor dimers could not be detected. Further aggregation can be induced by increasing the temperature to 340 K, lowering the pH to 5.0 or N-deglycosylation. Aggregation occurs according to a RLCA (reaction limited colloidal aggregation) scheme. Crystalline structures are formed during the temperature induced aggregation. Therefore a nucleation process must accompany the reaction limited aggregation. A simple modell for the aggreation of hTfR is given on the basis of DLVO theory. Interaction between hTfR and it´s ligand Transferrin were studied by fluorescence correlation spectroscopy. Binding and rate constants were determined. The experimental results are compatible with the binding of Transferrin to equal and independent binding sites

What do we really know about entrepreneurial orientation? Using quantitative empirical reviews to advance its concept and nomological network

In an overall effort to contribute to the steadily expanding EO literature, this cumulative dissertation aims to help the literature to advance with greater clarity, comprehensive modeling, and more robust research designs. To achieve this, the first paper of this dissertation focuses on the consistency and coherence in variable choices and modeling considerations by conducting a systematic quantitative review of the EO-performance literature. Drawing on the plethora of previous EO studies, the second paper employs a comprehensive meta-analytic structural equation modeling approach (MASEM) to explore the potential for unique component-level relationships among EO’s three core dimensions in antecedent to outcome relationships. The third paper draws on these component-level insights and performs a finer-grained replication of the seminal MASEM of Rosenbusch, Rauch, and Bausch (2013) that proposes EO as a full mediator between the task environment and firm performance. The fourth and final paper of this cumulative dissertation illustrates exigent endogeneity concerns inherent in observational EO-performance research and provides guidance on how researchers can move towards establishing causal relationships

What do we really know about entrepreneurial orientation? Using quantitative empirical reviews to advance its concept and nomological network

In an overall effort to contribute to the steadily expanding EO literature, this cumulative dissertation aims to help the literature to advance with greater clarity, comprehensive modeling, and more robust research designs. To achieve this, the first paper of this dissertation focuses on the consistency and coherence in variable choices and modeling considerations by conducting a systematic quantitative review of the EO-performance literature. Drawing on the plethora of previous EO studies, the second paper employs a comprehensive meta-analytic structural equation modeling approach (MASEM) to explore the potential for unique component-level relationships among EO’s three core dimensions in antecedent to outcome relationships. The third paper draws on these component-level insights and performs a finer-grained replication of the seminal MASEM of Rosenbusch, Rauch, and Bausch (2013) that proposes EO as a full mediator between the task environment and firm performance. The fourth and final paper of this cumulative dissertation illustrates exigent endogeneity concerns inherent in observational EO-performance research and provides guidance on how researchers can move towards establishing causal relationships

Forschendes Lernen im Fach Deutsch: Ein exemplarisches Forschungssetting für die Untersuchung von Lese- und Schreibprozessen

Schüler L. Forschendes Lernen im Fach Deutsch: Ein exemplarisches Forschungssetting für die Untersuchung von Lese- und Schreibprozessen. In: Winkel J, Busker M, Schüler L, Limberg H, Jaekel O, eds. Forschendes Lernen im Praxissemester an der Europa-Universität Flensburg. Erfahrungen - Empfehlungen - Perspektiven. Flensburg University Press; 2019: 41-60

Thermally Induced Aggregation of Human Transferrin Receptor Studied by Light-Scattering Techniques

AbstractThe thermal stability of transferrin receptor isolated from human placenta in detergent-free solution has been investigated by static light-scattering and photon correlation spectroscopy. In detergent-free solution at 293.2K, human transferrin receptor (hTfR) forms stable associates with a hydrodynamic radius of 16nm. With increasing temperature the particles get more compact, above 340K a phase transition takes, place and spontaneous aggregation of the receptor occurs. Under these conditions large clusters are formed that lead to fractal aggregates, coexisting with dendritic crystalline structures. The experimental findings are compatible with a model, which involves a reaction limited cluster-cluster aggregation mechanism in conjunction with a nucleation process. The molar enthalpy change associated with the phase transition was determined to be (1860±150) kJ/mol−1 at a transition temperature of (341.3±0.2) K