Aspects of nucleolar metabolism in herpesvirus infected cells

The inhibition of ribosomal RNA production has been studied in Hela cells infected with pseudorabies virus (PrV). This showed that both synthesis and processing of ribosomal RNA was affected. Processing in PrV-infected Hela cells was at a reduced rate but did follow a similar pattern to that of mock-infected cells. The effect on synthesis of ribosomal RNA was more pronounced and was followed by a concomitant decrease in the total nucleolar area of the infected Hela cells. Polyacrylamide gel electrophoresis of fractions from mock-infected and PrV-infected Hela cells showed that a number of virus induced polypeptides were associated with the nucleus, nucleolus and the nucleolar chromatin. Some of these polypeptides were immediate early viral gene products. A number of viral induced polypeptides were shown to be associated with the nuclei and nucleoli of mock-infected Hela cells after incubation in vitro of isolated nuclei and cell extracts of both mock-infected and infected Hela cells. The RNA polymerase activity of isolated nuclei of PrV- infected Hela cells was lower than that of mock-infected. This was true both for total and for a-amanitin resistant activities. The difference in RNA polymerase activity between mock-infected and infected Hela cells remained unchanged after incubation of the nuclei in the presence of the detergent sarkosyl. Again incubation in the presence of extracts of mock-infected or infected cells did not alter relative activities in the normal assay whereas RMA polymerase activity in mock-infected Hela cell nuclei was reduced after a combined exposure to infected cell extracts followed by treatment with aarkosyl. This suggests that its inhibition of ribosomal RNA synthesis in Hela cells after infection with pseudorabies virus is by interference at the initiation level of the transcription process rather than the elongation level. It may be mediated by a viral-induced protein(s) associated with the nucleolar and nucleolar chromatin cell fractions

Constraining anomalous Higgs boson couplings to virtual photons

We present a study of Higgs boson production in vector boson fusion and in association with a vector boson and its decay to two vector bosons, with a focus on the treatment of virtual loops and virtual photons. Our analysis is performed with the JHU generator framework. Comparisons are made to several other frameworks, and the results are expressed in terms of an effective field theory. New features of this study include a proposal on how to handle singularities involving Higgs boson decays to light fermions via photons, calculation of the partial Higgs boson width in the presence of anomalous couplings to photons, a comparison of the next-to-leading-order electroweak corrections to effects from effective couplings, and phenomenological observations regarding the special role of intermediate photons in analysis of LHC data in the effective field theory framework. Some of these features are illustrated with projections for experimental measurements with the full LHC and HL-LHC datasets.Comment: 32 pages, 14 figure

FPGA-Based Tracklet Approach to Level-1 Track Finding at CMS for the HL-LHC

During the High Luminosity LHC, the CMS detector will need charged particle tracking at the hardware trigger level to maintain a manageable trigger rate and achieve its physics goals. The tracklet approach is a track-finding algorithm based on a road-search algorithm that has been implemented on commercially available FPGA technology. The tracklet algorithm has achieved high performance in track-finding and completes tracking within 3.4 $\mu$s on a Xilinx Virtex-7 FPGA. An overview of the algorithm and its implementation on an FPGA is given, results are shown from a demonstrator test stand and system performance studies are presented.Comment: Submitted to proceedings of Connecting The Dots/Intelligent Trackers 2017, Orsay, Franc

Optimasi Portofolio Resiko Menggunakan Model Markowitz MVO Dikaitkan dengan Keterbatasan Manusia dalam Memprediksi Masa Depan dalam Perspektif Al-Qur`an

Risk portfolio on modern finance has become increasingly technical, requiring the use of sophisticated mathematical tools in both research and practice. Since companies cannot insure themselves completely against risk, as human incompetence in predicting the future precisely that written in Al-Quran surah Luqman verse 34, they have to manage it to yield an optimal portfolio. The objective here is to minimize the variance among all portfolios, or alternatively, to maximize expected return among all portfolios that has at least a certain expected return. Furthermore, this study focuses on optimizing risk portfolio so called Markowitz MVO (Mean-Variance Optimization). Some theoretical frameworks for analysis are arithmetic mean, geometric mean, variance, covariance, linear programming, and quadratic programming. Moreover, finding a minimum variance portfolio produces a convex quadratic programming, that is minimizing the objective function ðð¥with constraintsð ð 𥠥 ðandð´ð¥ = ð. The outcome of this research is the solution of optimal risk portofolio in some investments that could be finished smoothly using MATLAB R2007b software together with its graphic analysis

Measurement of the top quark mass using charged particles in pp collisions at root s=8 TeV

Peer reviewe

Measurement of t(t)over-bar normalised multi-differential cross sections in pp collisions at root s=13 TeV, and simultaneous determination of the strong coupling strength, top quark pole mass, and parton distribution functions

Peer reviewe

MUSiC : a model-unspecific search for new physics in proton-proton collisions at root s=13TeV

Results of the Model Unspecific Search in CMS (MUSiC), using proton-proton collision data recorded at the LHC at a centre-of-mass energy of 13 TeV, corresponding to an integrated luminosity of 35.9 fb(-1), are presented. The MUSiC analysis searches for anomalies that could be signatures of physics beyond the standard model. The analysis is based on the comparison of observed data with the standard model prediction, as determined from simulation, in several hundred final states and multiple kinematic distributions. Events containing at least one electron or muon are classified based on their final state topology, and an automated search algorithm surveys the observed data for deviations from the prediction. The sensitivity of the search is validated using multiple methods. No significant deviations from the predictions have been observed. For a wide range of final state topologies, agreement is found between the data and the standard model simulation. This analysis complements dedicated search analyses by significantly expanding the range of final states covered using a model independent approach with the largest data set to date to probe phase space regions beyond the reach of previous general searches.Peer reviewe

Measurement of prompt open-charm production cross sections in proton-proton collisions at root s=13 TeV

The production cross sections for prompt open-charm mesons in proton-proton collisions at a center-of-mass energy of 13TeV are reported. The measurement is performed using a data sample collected by the CMS experiment corresponding to an integrated luminosity of 29 nb(-1). The differential production cross sections of the D*(+/-), D-+/-, and D-0 ((D) over bar (0)) mesons are presented in ranges of transverse momentum and pseudorapidity 4 < p(T) < 100 GeV and vertical bar eta vertical bar < 2.1, respectively. The results are compared to several theoretical calculations and to previous measurements.Peer reviewe

Search for new particles in events with energetic jets and large missing transverse momentum in proton-proton collisions at root s=13 TeV

A search is presented for new particles produced at the LHC in proton-proton collisions at root s = 13 TeV, using events with energetic jets and large missing transverse momentum. The analysis is based on a data sample corresponding to an integrated luminosity of 101 fb(-1), collected in 2017-2018 with the CMS detector. Machine learning techniques are used to define separate categories for events with narrow jets from initial-state radiation and events with large-radius jets consistent with a hadronic decay of a W or Z boson. A statistical combination is made with an earlier search based on a data sample of 36 fb(-1), collected in 2016. No significant excess of events is observed with respect to the standard model background expectation determined from control samples in data. The results are interpreted in terms of limits on the branching fraction of an invisible decay of the Higgs boson, as well as constraints on simplified models of dark matter, on first-generation scalar leptoquarks decaying to quarks and neutrinos, and on models with large extra dimensions. Several of the new limits, specifically for spin-1 dark matter mediators, pseudoscalar mediators, colored mediators, and leptoquarks, are the most restrictive to date.Peer reviewe