Numerical simulation of the under-expanded flow in the experimental conical nozzle helios-x

Estudios numéricos del campo de flujo para toberas convergentes-divergentes con longitud de garganta han reportado fluctuaciones del flujo con ondas de choque oblicuo en la sección de la garganta, para la condición de flujo sobreexpandido. Sin embargo, para otras condiciones del flujo, para un mismo tipo de tobera, el conocimiento es limitado. En el presente trabajo, el objetivo es determinar el comportamiento del flujo en la longitud de garganta y en la divergente, para una tobera cónica experimental clasificada como Helios-X, para la condición de flujo subexpandido. Se realizaron simulaciones numéricas 2D del campo de flujo con el código ANSYS-Fluent versión 12.1, aplicando el modelo RANS. Se emplearon las ecuaciones gobernantes para el flujo compresible, conservación de la masa, cantidad de movimiento, energía y de estado; así como, para la turbulencia el modelo SST  de Menter y para la viscosidad en función de la temperatura la ecuación de Sutherland. En la sección de la garganta, adyacente a la pared, el flujo presentó fluctuaciones, en la simetría axial el flujo presentó una aceleración escalonada; en la sección divergente, el flujo se desaceleró en cierta región, sin embargo, el flujo salió de la tobera a velocidad supersónica ligeramente mayor de Mach 3. Se concluye que en la sección de la longitud de garganta se presenta un patrón de flujo, así como, en la sección divergente.Numerical studies of the flow field for convergent-divergent nozzles with throat length, have reported fluctuations of the flow with oblique shock waves in the throat section, for the over-expanded flow condition. However, for other flow conditions, for the same type of nozzle, knowledge is limited. In the present work, the objective is to determine the behavior of the flow in the throat length and in the divergent, for an experimental conical nozzle classified as Helios-X, for the under-expanded flow condition. 2D numerical simulations of the flow field were performed with the ANSYS-Fluent version 12.1 code, applying the RANS model. The governing equations for compressible flow, conservation of mass, momentum, energy, and state were used; as well as, for turbulence, the Menter model SST  and for the viscosity as a function of temperature the Sutherland equation. In the section of the throat, adjacent to the wall, the flow presented fluctuations, in the axial symmetry the flow presented a stepped acceleration; in the divergent section, the flow slowed in a certain region, however, the flow exited the nozzle at a supersonic speed slightly greater than Mach 3. It is concluded that in the throat length section there is a flow pattern, as well as, in the divergent section

Simulación numérica del flujo sub-expandido en la tobera cónica experimental helios-x

Estudios numéricos del campo de flujo para toberas convergentes-divergentes con longitud de garganta, han reportado fluctuaciones del flujo con ondas de choque oblicuo en la sección de la garganta, para la condición de flujo sobre-expandido. Sin embargo, para otras condiciones del flujo, para un mismo tipo de tobera, el conocimiento es limitado. En el presente trabajo, el objetivo es determinar el comportamiento del flujo en la longitud de garganta y en la divergente, para una tobera cónica experimental clasificada como Helios-X, para la condición de flujo sub-expandido. Se realizaron simulaciones numéricas 2D del campo de flujo con el código ANSYS-Fluent versión 12.1, aplicando el modelo RANS. Se emplearon las ecuaciones gobernantes para el flujo compresible, conservación de la masa, cantidad de movimiento, energía y de estado; así como, para la turbulencia el modelo SST k − ω de Menter y para la viscosidad en función de la temperatura la ecuación de Sutherland. En la sección de la garganta, adyacente a la pared, el flujo presentó fluctuaciones, en la simetría axial el flujo presentó una aceleración escalonada; en la sección divergente, el flujo se desaceleró en cierta región, sin embargo, el flujo salió de la tobera a velocidad supersónica ligeramente mayor de Mach 3. Se concluye que en la sección de la longitud de garganta se presenta un patrón de flujo, así como, en la sección divergente.//Numerical studies of the flow field for convergentdivergent nozzles with throat length, have reported fluctuations of the flow with oblique shock waves in the throat section, for the over-expanded flow condition. However, for other flow conditions, for the same type of nozzle, knowledge is limited. In the present work, the objective is to determine the behavior of the flow in the throat length and in the divergent, for an experimental conical nozzle classified as Helios-X, for the under-expanded flow condition. 2D numerical simulations of the flow field were performed with the ANSYS-Fluent version 12.1 code, applying the RANS model. The governing equations for compressible flow, conservation of mass, momentum, energy, and state were used; as well as, for turbulence, the Menter model SST k − ω and for the viscosity as a function of temperature the Sutherland equation. In the section of the throat, adjacent to the wall, the flow presented fluctuations, in the axial symmetry the flow presented a stepped acceleration; in the divergent section, the flow slowed in a certain region, however, the flow exited the nozzle at a supersonic speed slightly greater than Mach 3. It is concluded that in the throat length section there is a flow pattern, as well as, in the divergent section

Simulación numérica del flujo subexpandido en la tobera cónica experimental helios-x

Numerical studies of the flow field for convergent-divergent nozzles with throat length, have reported fluctuations of the flow with oblique shock waves in the throat section, for the over-expanded flow condition. However, for other flow conditions, for the same type of nozzle, knowledge is limited. In the present work, the objective is to determine the behavior of the flow in the throat length and in the divergent, for an experimental conical nozzle classified as Helios-X, for the under-expanded flow condition. 2D numerical simulations of the flow field were performed with the ANSYS-Fluent version 12.1 code, applying the RANS model. The governing equations for compressible flow, conservation of mass, momentum, energy, and state were used; as well as, for turbulence, the Menter model SST  and for the viscosity as a function of temperature the Sutherland equation. In the section of the throat, adjacent to the wall, the flow presented fluctuations, in the axial symmetry the flow presented a stepped acceleration; in the divergent section, the flow slowed in a certain region, however, the flow exited the nozzle at a supersonic speed slightly greater than Mach 3. It is concluded that in the throat length section there is a flow pattern, as well as, in the divergent section.Estudios numéricos del campo de flujo para toberas convergentes-divergentes con longitud de garganta han reportado fluctuaciones del flujo con ondas de choque oblicuo en la sección de la garganta, para la condición de flujo sobreexpandido. Sin embargo, para otras condiciones del flujo, para un mismo tipo de tobera, el conocimiento es limitado. En el presente trabajo, el objetivo es determinar el comportamiento del flujo en la longitud de garganta y en la divergente, para una tobera cónica experimental clasificada como Helios-X, para la condición de flujo subexpandido. Se realizaron simulaciones numéricas 2D del campo de flujo con el código ANSYS-Fluent versión 12.1, aplicando el modelo RANS. Se emplearon las ecuaciones gobernantes para el flujo compresible, conservación de la masa, cantidad de movimiento, energía y de estado; así como, para la turbulencia el modelo SST  de Menter y para la viscosidad en función de la temperatura la ecuación de Sutherland. En la sección de la garganta, adyacente a la pared, el flujo presentó fluctuaciones, en la simetría axial el flujo presentó una aceleración escalonada; en la sección divergente, el flujo se desaceleró en cierta región, sin embargo, el flujo salió de la tobera a velocidad supersónica ligeramente mayor de Mach 3. Se concluye que en la sección de la longitud de garganta se presenta un patrón de flujo, así como, en la sección divergente

The Role of FAS Receptor Methylation in Osteosarcoma Metastasis.

Osteosarcoma is the most frequent primary malignant bone tumor with an annual incidence of about 400 cases in the United States. Osteosarcoma primarily metastasizes to the lungs, where FAS ligand (FASL) is constitutively expressed. The interaction of FASL and its cell surface receptor, FAS, triggers apoptosis in normal cells; however, this function is altered in cancer cells. DNA methylation has previously been explored as a mechanism for altering FAS expression, but no variability was identified in the CpG island (CGI) overlapping the promoter. Analysis of an expanded region, including CGI shores and shelves, revealed high variability in the methylation of certain CpG sites that correlated significantly with FAS mRNA expression in a negative manner. Bisulfite sequencing revealed additional CpG sites, which were highly methylated in the metastatic LM7 cell line but unmethylated in its parental non-metastatic SaOS-2 cell line. Treatment with the demethylating agent, 5-azacytidine, resulted in a loss of methylation in CpG sites located within the FAS promoter and restored FAS protein expression in LM7 cells, resulting in reduced migration. Orthotopic implantation of 5-azacytidine treated LM7 cells into severe combined immunodeficient mice led to decreased lung metastases. These results suggest that DNA methylation of CGI shore sites may regulate FAS expression and constitute a potential target for osteosarcoma therapy, utilizing demethylating agents currently approved for the treatment of other cancers

Cdk5: Multitasking between physiological and pathological conditions

Cyclin-dependent kinase 5 (Cdk5) is a peculiar proline-directed serine/threonine kinase. Unlike the other members of the Cdk family, Cdk5 is not directly involved in cell cycle regulation, being normally associated with neuronal processes such as migration, cortical layering and synaptic plasticity. This kinase is present mainly in post-mitotic neurons and its activity is tightly regulated by the interaction with the specific activators, p35 and p39. Despite its pivotal role in CNS development, Cdk5 dysregulation has been implicated in different pathologies, such as Alzheimer's disease (AD), amyotrophic lateral sclerosis (ALS), Parkinson's disease (PD) and, most recently, prion-related encephalopathies (PRE). In these neurodegenerative conditions, Cdk5 overactivation and relocalization occurs upon association with p25, a truncated form of the normal activator p35. This activator switching will cause a shift in the phosphorylative pattern of Cdk5, with an alteration both in targets and activity, ultimately leading to neuronal demise. In AD and PRE, two disorders that share clinical and neuropathological features, Cdk5 dysregulation is a linking event between the major neuropathological markers: amyloid plaques, tau hyperphosphorylation and synaptic and neuronal loss. Moreover, this kinase was shown to be involved in abortive cell cycle re-entry, a feature recently proposed as a possible step in the neuronal apoptosis mechanism of several neurological diseases. This review focuses on the role of Cdk5 in neurons, namely in the regulation of cytoskeletal dynamics, synaptic function and cell survival, both in physiological and in pathological conditions, highlighting the relevance of Cdk5 in the main mechanisms of neurodegeneration in Alzheimer's disease and other brain pathologies

2023 EELS field tests at Athabasca Glacier as an icy moon analogue environment

JPL is developing a versatile and highly intelligent Exobiology Extant Life Surveyor (EELS) robot that would enable access to subsurface oceans and near-surface liquid reservoirs through existing conduits, such as the vents at the south pole of Enceladus or the putative geysers on Europa. A key mobility requirement for future vent exploration missions will be the ability to carefully descend and hold position in the vent to collect and analyze samples while withstanding plume forces without human intervention. Furthermore, this must be accomplished in a highly uncertain environment, requiring versatile hardware and intelligent autonomy. To work towards that goal, we have prototyped the EELS 1.0 and EELS 1.5 robots for horizontal and vertical mobility, respectively, in icy terrain. Autonomous surface mobility of EELS 1.0 was previously validated in a variety of terrain, including snowy mountains, ice rinks, and desert sand. Vertical mobility of EELS 1.5 was developed on laboratory ice walls. This paper presents the first mobility trials for both robots on large-scale, natural icy terrain: the Athabasca Glacier located in Alberta, Canada, a terrestrial analogue to the surfaces and subsurfaces of icy moons. This paper provides a preliminary written record of the test campaign’s four major trials: 1) surface mobility with EELS 1.0, 2) vertical mobility with EELS 1.5, 3) science instrument validation, and 4) terramechanics experiments. During this campaign, EELS 1.5 successfully held position and descended ~1.5 m vertically in an icy conduit and EELS 1.0 demonstrated surface mobility on icy surfaces with undulations and slopes. A miniaturized capillary electrophoresis (CE) instrument built to the form factor of an EELS module was tested in flowing water on the glacier and successfully demonstrated automated sampling and in-situ analysis. Terramechanics experiments designed to better understand the interaction between different ice properties and the screws that propel the robot forwards were performed on horizontal and vertical surfaces. In this paper we report the outcomes of the four tests and discuss their implications for potential future icy missions. The field test also demonstrated EELS’s ability to support Earth science missions. Another potential near-term follow-on could be a technology demonstration on the Moon. This paper is a high level report on the execution of the field test. Data and results will be detailed in subsequent publications