Exploring Lifestyle Habits, Physical Activity, Anxiety and Basic Psychological Needs in a Sample of Portuguese Adults during COVID-19

This cross-sectional study aimed to characterize the lifestyle habits, anxiety levels and basic psychological needs (BPN), in Portuguese adults during the Coronavirus Disease 2019 (COVID-19) pandemic, including a comparison between genders and age groups. In total, 1404 adults (36.4 ± 11.7 years; 69.6% female) answered sociodemographic data and three instruments: the International Physical Activity Questionnaire, the Basic Need General Satisfaction Scale and the State-Trait Anxiety Inventory. Males revealed higher values for the total energy expenditure (z = -2.26; p = 0.024; η 2 = 0.004) and for the level of satisfaction of competence (z = -2.62; p = 0.009; η 2 = 0.005). Females showed higher scores for the anxiety state (z = -7.87; p ≤ 0.001; η 2 = 0.044) and anxiety trait (z = -6.49; p ≤ 0.001; η 2 = 0.030). Regarding age, higher values for the anxiety trait (p ≤ 0.001; η H 2 = 0.030) were found in the 18-34 years-old group compared to all the other age groups, also presenting significantly higher values of total energy expenditure (χ² = 13.93; p = 0.008; η H 2 = 0.007) when compared to the 35-44 years-old group. Significant differences were observed between the 18-34 years-old group and the other age groups for the satisfaction of competence (χ² = 40.97; p ≤ 0.001; η H 2 = 0.026), except for the >65 years-old group. Strategies for promoting well-being during periods of social isolation should consider the role of psychological dimensions and lifestyle habits according to the gender or age group.info:eu-repo/semantics/publishedVersio

Impact of Early Postnatal Androgen Exposure on Voice Development

Background: the impact of early postnatal androgen exposure on female laryngeal tissue may depend on certain characteristics of this exposure. We assessed the impact of the dose, duration, and timing of early androgen exposure on the vocal development of female subjects who had been treated for adrenocortical tumor (ACT) in childhood.Methods: the long-term effects of androgen exposure on the fundamental vocal frequency (F0), vocal pitch, and final height and the presence of virilizing signs were examined in 9 adult (age, 18.4 to 33.5 years) and 10 adolescent (13.6 to 17.8 years) female ACT patients. We also compared the current values with values obtained 0.9 years to 7.4 years after these subjects had undergone ACT surgery, a period during which they had shown normal androgen levels.Results: of the 19 subjects, 17 (89%) had been diagnosed with ACT before 4 years of age, 1 (5%) at 8.16 years, and 1 (5%) at 10.75 years. Androgen exposure (2 to 30 months) was sufficiently strong to cause pubic hair growth in all subjects and clitoromegaly in 74% (14/19) of the subjects, but did not reduce their height from the target value. Although androgen exposure induced a remarkable reduction in F0 (132 Hz) and moderate pitch virilization in 1 subject and partial F0 virilization, resulting in F0 of 165 and 169 Hz, in 2 subjects, the majority had normal F0 ranging from 189 to 245 Hz.Conclusions: Female laryngeal tissue is less sensitive to androgen exposure between birth and adrenarche than during other periods. Differential larynx sensitivity to androgen exposure in childhood and F0 irreversibility in adulthood are age-, concentration-, duration-, and timing-dependent events that may also be affected by exposure to inhibitory or stimulatory hormones. Further studies are required to better characterize each of these factors.Brazilian State of Parana Secretary of Science, Technology and Higher Education (SETI)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Raul Carneiro Hospital Association for Childhood Protection (AHPIRC)American Lebanese Syrian Associated Charities (ALSAC)SETIAHPIRCALSACInst Pesquisa Pele Pequeno Principe, Curitiba, Parana, BrazilHosp Pequeno Principe, Curitiba, Parana, BrazilFac Pequeno Principe, Curitiba, Parana, BrazilInst Voz Maringa, Maringa, Parana, BrazilUniversidade Federal de São Paulo, Dept Fonoaudiol, São Paulo, BrazilClin Voz, Curitiba, Parana, BrazilIrmandade Santa Casa Misericordia São Paulo, São Paulo, BrazilUniv Fed Parana, Dept Saude Comunitaria, BR-80060000 Curitiba, Parana, BrazilUniversidade Federal de São Paulo, Dept Fonoaudiol, São Paulo, BrazilWeb of Scienc

Libidibia ferrea loaded in bacterial nanocellulose: evaluation of antimicrobial activity and wound care / Libidibia ferrea loaded in bacterial nanocellulose: evaluation of antimicrobial activity and wound care

The effects of Bacterial Nanocellulose (BNC) loaded with Libidibia ferrea (Lf) hydroalcoholic extract were investigated on the healing process of burn in diabetic and non-diabetic animals. In vivo assay was performed with 36 male rats, with streptozotocin-induced diabetes and burns induced by contact. Animals were divided into Nd-BNC (Non-diabetic + Bacterial nanocellulose membranes); Nd-BNC-Lf (Non-diabetic + Bacterial nanocellulose membranes + Libidibia ferrea); D-BNC (Diabetic + Bacterial nanocellulose membranes); D-BNC-Lf (Diabetic + Bacterial nanocellulose membranes + Libidibia ferrea). Wounds were evaluated for 28 days histologically. Lf extract and BNC-Lf extract showed antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The severe degree of infection, granulation and inflammation observed after 14 days in diabetic rats (exposed or not to Lf extract), disappeared after 21 days. On the 28th day, there was no histological difference among the groups. BNC-Lf extract demonstrated to have antimicrobial activity, however as an wound dressing, both BNC or BNC-Lf extract were effective in the healing of second-degree burn wounds

Discrete moving frames on lattice varieties and lattice based multispace

In this paper, we develop the theory of the discrete moving frame in two different ways. In the first half of the paper, we consider a discrete moving frame defined on a lattice variety and the equivalence classes of global syzygies that result from the first fundamental group of the variety. In the second half, we consider the continuum limit of discrete moving frames as a local lattice coalesces to a point. To achieve a well-defined limit of discrete frames, we construct multispace, a generalization of the jet bundle that also generalizes Olver’s one dimensional construction. Using interpolation to provide coordinates, we prove that it is a manifold containing the usual jet bundle as a submanifold. We show that continuity of a multispace moving frame ensures that the discrete moving frame converges to a continuous one as lattices coalesce. The smooth frame is, at the same time, the restriction of the multispace frame to the embedded jet bundle. We prove further that the discrete invariants and syzygies approximate their smooth counterparts. In effect, a frame on multispace allows smooth frames and their discretisations to be studied simultaneously. In our last chapter we discuss two important applications, one to the discrete variational calculus, and the second to discrete integrable systems. Finally, in an appendix, we discuss a more general result concerning equicontinuous families of discretisations of moving frames, which are consistent with a smooth frame

A large outbreak of Legionnaires’ Disease in an industrial town in Portugal

Background We describe the investigation and control of an outbreak of Legionnaires’ disease in Portugal in October, November and December 2014. Methods Confirmed cases were individuals with pneumonia, laboratory evidence of Legionella pneumophila serogroup 1 and exposure, by residence, occupational or leisure to the affected municipalities. 49 possible sources were reduced to four potential sources, all industries with wet cooling system, following risk assessment. We geo-referenced cases’ residences and the location of cooling towers defining four study areas 10 km buffer centered on each cooling tower system. We compared the number of cases with expected numbers, calculated from the outbreak's attack rates applied to 2011 census population. Using Stones’ Test, we tested observed to expected ratios for decline in risk, with distance up to 10 km four directions. Isolates of Legionella pneumophila were compared using molecular methods. Results We identified 403 cases, 377 of which were confirmed, 14 patients died. Patients became ill between 14 October and 2 December. A NE wind and thermal inversion were recorded during the estimated period of exposure. Disease risk was highest in people living south west from all of the industries identified and decreased with distance (p < 0.001). 71 clinical isolates demonstrated an identical SBT profile to an isolate from a cooling tower. Whole genome sequencing identified an unusual L. pneumophila subsp. fraseri serogroup 1 as the outbreak causative strain, and confirmed isolates’ relatedness. Conclusions Industrial wet cooling systems, bacteria with enhanced survival characteristics and a combination of climatic conditions contributed to the second largest outbreak of Legionnaires’ disease recorded internationally.info:eu-repo/semantics/publishedVersio

Futsal as a potential talent development modality for soccer - a quantitative assessment of high-level soccer coach and player perceptions

A contemporary issue in soccer talent development is the potential use of futsal as a training tool. This paper utilised survey methods to quantitatively assess the perspectives of high-level coaches working in youth player education, and players of futsal and 11-a-side soccer (n=77). Respondents completed surveys before and following a showcase game between an Under-21 international futsal team competing against a Premier League Under-21 soccer team. Responses were positive about futsal generally as a training aid and specifically, skills transfer from futsal into soccer performance. The majority of respondents (89.6%) indicated ‘post-game’ that they considered futsal could be useful for soccer talent development. Similarly, 90.9% stated they would consider its integration in their training/games program. Analysis of opinions on futsal regarding its constraints and the impact its specific rules may have on skill acquisition generally reported positive perceptions: 89.6% of participants suggested that pitch size improved skills (strongly agree=33.8%, agree=55.8%), 88.3% believed the pass back rule enhanced ball reception skills (strongly agree=27.3%, agree=61%), and 89.2% perceived that futsal can help develop ‘multifunctional’ players (strongly agree=27.3%, agree=62.3%). This knowledge of the constraints and potential for skill acquisition linked to futsal participation is a first step in aiding soccer coaches to understand potential learning returns from its inclusion in talent development policies and programs

Match-Play and Performance Test Responses of Soccer Goalkeepers: A Review of Current Literature.

Goalkeepers are typically the last defensive line for soccer teams aiming to minimise goals being conceded, with match rules permitting ball handling within a specific area. Goalkeepers are also involved in initiating some offensive plays, and typically remain in close proximity to the goal line while covering ~ 50% of the match distances of outfield players; hence, the competitive and training demands of goalkeepers are unique to their specialised position. Indeed, isolated performance tests differentiate goalkeepers from outfield players in multiple variables. With a view to informing future research, this review summarised currently available literature reporting goalkeeper responses to: (1) match play (movement and skilled/technical demands) and (2) isolated performance assessments (strength, power, speed, aerobic capacity, joint range of motion). Literature searching and screening processes yielded 26 eligible records and highlighted that goalkeepers covered ~ 4-6 km on match day whilst spending ~ 98% of time at low-movement intensities. The most decisive moments are the 2-10 saves·match-1 performed, which often involve explosive actions (e.g. dives, jumps). Whilst no between-half performance decrements have been observed in professional goalkeepers, possible transient changes over shorter match epochs remain unclear. Isolated performance tests confirm divergent profiles between goalkeepers and outfield players (i.e. superior jump performance, reduced [Formula: see text]2max values, slower sprint times), and the training of soccer goalkeepers is typically completed separately from outfield positions with a focus primarily on technical or explosive drills performed within confined spaces. Additional work is needed to examine the physiological responses to goalkeeper-specific training and match activities to determine the efficacy of current preparatory strategies

The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability

Chromobacterium violaceum is one of millions of species of free-living microorganisms that populate the soil and water in the extant areas of tropical biodiversity around the world. Its complete genome sequence reveals (i) extensive alternative pathways for energy generation, (ii) ≈500 ORFs for transport-related proteins, (iii) complex and extensive systems for stress adaptation and motility, and (iv) wide-spread utilization of quorum sensing for control of inducible systems, all of which underpin the versatility and adaptability of the organism. The genome also contains extensive but incomplete arrays of ORFs coding for proteins associated with mammalian pathogenicity, possibly involved in the occasional but often fatal cases of human C. violaceum infection. There is, in addition, a series of previously unknown but important enzymes and secondary metabolites including paraquat-inducible proteins, drug and heavy-metal-resistance proteins, multiple chitinases, and proteins for the detoxification of xenobiotics that may have biotechnological applications