Alergia à proteína do leite de vaca, qualidade de vida e estilos parentais

Introduction: Cow’s milk protein allergy requires changes in family habits to maintain children’s health. Objective: This study evaluated the effects of cow’s milk protein allergy on the health of children, the quality of life of parents and children, and the adopted parental styles. Methods: Control case study. The case group consisted of children with cow’s milk protein allergy, from eight months to five years old, and those guardians, and the Control Group, for healthy children of the same age group, and their parents. The quality of life of the child (TNO-AZL Preschool Children Quality of Life) and the caregiver (SF-36) were evaluated; parental style (Parental Beliefs and Care Practices Scale); and socioeconomic and health data of the child. The Mann-Whitney test was used to compare the groups (p <0.05). Results: 76 dyads from the case group and 44 from the control group participated. Children with cow’s milk protein allergy had a lower quality of life in the health dimension, worse nutritional status, followed up with a larger number of health professionals. Those in charge of the case group offered less body stimulation to the children. Those in the control group had a lower quality of emotional life. Conclusions: Cow’s milk protein allergy had an impact on the health and nutritional status of children, on the corporal stimulation received by the children, and on the quality of emotional life of those guardians.Introdução: A alergia à proteína do leite de vaca requer alterações dos hábitos familiares para manutenção das condições de saúde das crianças. Objetivo: Este estudo analisou os efeitos da alergia à proteína do leite de vaca sobre a saúde de crianças, qualidade de vida de responsáveis e crianças e sobre os estilos parentais adotados. Método: Estudo caso controle. O grupo caso foi constituído por crianças com alergia à proteína do leite de vaca, de oito meses a cinco anos de idade, e seus responsáveis, e o Grupo Controle, por crianças saudáveis, de mesma faixa etária, e seus responsáveis. Foram avaliadas a qualidade de vida da criança (TNO-AZL Preschool Children Quality of Life) e do responsável (SF-36); estilo parental (Escala de Crenças Parentais e Práticas de Cuidado); e dados socioeconômicos e de saúde da criança. O teste de Mann-Whitney foi utilizado para comparar os grupos (p <0,05). Resultados: Participaram 26 díades do grupo caso e 44 do grupo controle. Crianças com alergia proteína do leite de vaca apresentaram menor qualidade de vida na dimensão saúde, pior estado nutricional, realizaram acompanhamento com maior número de profissionais da saúde. Os responsáveis do grupo caso ofereceram menor estimulação corporal às crianças. Responsáveis do grupo controle apresentaram menor qualidade de vida emocional. Conclusão: A alergia à proteína do leite de vaca impactou na saúde e no estado nutricional das crianças, na estimulação corporal recebida pelas crianças, e na qualidade de vida emocional dos responsáveis

Chloroplast genome assembly of Serjania erecta Raldk: comparative analysis reveals gene number variation and selection in protein-coding plastid genes of Sapindaceae

Serjania erecta Raldk is an essential genetic resource due to its anti-inflammatory, gastric protection, and anti-Alzheimer properties. However, the genetic and evolutionary aspects of the species remain poorly known. Here, we sequenced and assembled the complete chloroplast genome of S. erecta and used it in a comparative analysis within the Sapindaceae family. S. erecta has a chloroplast genome (cpDNA) of 159,297 bp, divided into a Large Single Copy region (LSC) of 84,556 bp and a Small Single Copy region (SSC) of 18,057 bp that are surrounded by two Inverted Repeat regions (IRa and IRb) of 28,342 bp. Among the 12 species used in the comparative analysis, S. erecta has the fewest long and microsatellite repeats. The genome structure of Sapindaceae species is relatively conserved; the number of genes varies from 128 to 132 genes, and this variation is associated with three main factors: (1) Expansion and retraction events in the size of the IRs, resulting in variations in the number of rpl22, rps19, and rps3 genes; (2) Pseudogenization of the rps2 gene; and (3) Loss or duplication of genes encoding tRNAs, associated with the duplication of trnH-GUG in X. sorbifolium and the absence of trnT-CGU in the Dodonaeoideae subfamily. We identified 10 and 11 mutational hotspots for Sapindaceae and Sapindoideae, respectively, and identified six highly diverse regions (tRNA-Lys — rps16, ndhC – tRNA-Val, petA – psbJ, ndhF, rpl32 – ccsA, and ycf1) are found in both groups, which show potential for the development of DNA barcode markers for molecular taxonomic identification of Serjania. We identified that the psaI gene evolves under neutrality in Sapindaceae, while all other chloroplast genes are under strong negative selection. However, local positive selection exists in the ndhF, rpoC2, ycf1, and ycf2 genes. The genes ndhF and ycf1 also present high nucleotide diversity and local positive selection, demonstrating significant potential as markers. Our findings include providing the first chloroplast genome of a member of the Paullinieae tribe. Furthermore, we identified patterns in variations in the number of genes and selection in genes possibly associated with the family’s evolutionary history

Search for direct production of charginos and neutralinos in events with three leptons and missing transverse momentum in √s = 7 TeV pp collisions with the ATLAS detector

A search for the direct production of charginos and neutralinos in final states with three electrons or muons and missing transverse momentum is presented. The analysis is based on 4.7 fb−1 of proton–proton collision data delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in three signal regions that are either depleted or enriched in Z-boson decays. Upper limits at 95% confidence level are set in R-parity conserving phenomenological minimal supersymmetric models and in simplified models, significantly extending previous results