First bioimpedance assessment for detecting eects caused by environmental stimuli on Grapevine (Vitis vinifera L.): preliminary results

Plants comprise biological tissues where bioimpedance could be measured.Changes in values of these impedance parameters within tissues are the result of changes inthe chemical composition of the sap, cellular depolarization, and ion transport in response toexternal stimuli such as water availability, diseases, and photosynthesis activity, among others.Therefore, the environmental eects on the plant change its morphology and physiology wouldbe related to bioimpedance. In this way, bioimpedance could become a novel and powerfultechnique used to analyze, in-situ and in real-time, the physiologic activity and status. In thisstudy, we assess the Evaluation Board AD5933EBZ as a bioimpedance low-cost measurementdevice to perform observations of module and phase of the bioimpedance, aimed to correlatethem with the eects of environmental stimuli such as irrigation, CO2 concentration, sunexposure and temperature level in the grapevine.Fil: Barboni, Leonardo. Universidad de la Republica. Facultad de Ingeniería; UruguayFil: Fourment, Mercedes. Universidad de la Republica. Facultad de Agricultura; UruguayFil: Tachini, Ramiro. Universidad de la Republica. Facultad de Agricultura; UruguayFil: Martinez, Mauro Sebastian. Universidad de la Republica. Facultad de Agricultura; UruguayFil: Quero, Gaston. Universidad de la Republica. Facultad de Agricultura; UruguayFil: Dell'osa, Antonio Héctor. Universidad Nacional de Tierra del Fuego. Instituto de Desarrollo Economico E Innovacion; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Austral de Investigaciones Científicas; Argentina4th Latin American Conference on BioimpedanceSan Luis PotosíMéxicoSocieda Mexicana de Ingeniería Biomédic

Variation in the species richness of parasitoid wasps (Ichneumonidae: Pimplinae and Rhyssinae) across sites on different continents

The old idea that parasitoid wasps (Ichneumonidae) show an inverse latitudinal diversity gradient has recently been challenged, but how ichneumonid species richness varies across the globe is still not well understood. We carried out field inventories in 21 sites on three continents to clarify this question, focusing on the subfamilies Pimplinae and Rhyssinae. Our total sampling effort was 628 Malaise trap months and the total catch exceeded 65 000 individuals. Our main focus was in two intensively inventoried areas in Amazonia, together yielding 257 Malaise trap months and 26 390 ichneumonid individuals. To expand the scope and assess global species diversity patterns of the Pimplinae and Rhyssinae, we compiled published species lists from a total of 97 study localities around the world. The highest observed species richness in any locality, 105 species, was found in one of our field sites in Peruvian Amazonia. None of the other localities reported more than 70 species, even the ones with a sampling effort comparable to ours. Despite the local thoroughness of our field inventories in Amazonia, data analyses indicated that a substantial proportion of the parasitoid wasp species occurring in each site remained unobserved. The highest local species richness values were reported from the tropics. Nevertheless parasitoid wasps are still too sparsely sampled to draw solid conclusions about whether or not their species richness follows a particular latitudinal trend, and if so, where their richness peaks.The study was partly funded by the Kone Foundation, Finland (a grant awarded to the project: Biodiversity and multiple trophic interactions lead by Ilari E. Sääksjärvi). Isrrael C. Gómez thanks the support of the following foundations: the Amazon Conservation Association (Peru), Conservation International Foundation‐Peru and the Turku University Foundation. Marc Pollet is indebted to the Leopold III Fund (Belgium) for providing support for the Chilean survey. Field work in Los Queules and Huinay (Chile) was funded by research projects FONDECYT 1050745 and “Ayudas para la realización de estancias de investigación en el centro científico de la Fundación Huinay” (CSIC‐Endesa), grants to J. L. Nieves‐Aldrey

Electrochemical biosensor for glycine detection in biological fluids

We present herein the very first amperometric biosensor for the quantitative determination of glycine in diverse biological fluids. The biosensor is based on a novel quinoprotein that catalyzes the oxidation of glycine with high specificity. This process is coupled to the redox conversion of Prussian blue in the presence of hydrogen peroxide originating from the enzymatic reaction. The optimized tailoring of the biosensor design consists of the effective encapsulation of the quinoprotein in a chitosan matrix with the posterior addition of an outer Nafion layer, which is here demonstrated to suppress matrix interference. This is particularly important in the case of ascorbic acid, which is known to influence the redox behavior of the Prussian blue. The analytical performance of the biosensor demonstrates fast response time (&lt;7 s), acceptable reversibility, reproducibility, and stability (&lt;6% variation) as well as a wide linear range of response (25?500 ?M) that covers healthy (and even most unhealthy) physiological levels of glycine in blood/serum, urine and sweat. A total of 6 real samples from healthy patients and animals were analyzed: two serum, two urine and two sweat samples. The results were validated via commercially available fluorescence kit, displaying discrepancy of less than 9% in all the samples. The unique analytical features and effortless preparation of the new glycine biosensor position it at the forefront of current technologies towards decentralized clinical applications and sport performance monitoring.QC 20210524</p

QTLs detection for phenological traits within the Rosaceae family.

International audienc

Patterns in species size, size, and latitudinal range of East Atlantic fishes: Depth and latitudinal covariation.

7 páginas, 5 figuras, 2 tablas.Examination of latitudinal patterns in species richness, size, and distributional range of East Atlantic fish, based on a compilation of data encompassing the full latitudinal and depth distribution of 1746 East Atlantic fish species, showed that species richness declined towards higher latitudes at a rate of c. I% of the number of species present, in five-degree bands, for each degree of latitude for both te1eosts and elasmobranchs, regardless of habitat. However, the latitudinal patterns in maximum fish size and latitudinal range differed between teleosts and elasmobranchs, and changed with habitat. No clear evidence was obtained that the latitudinal range occupied increased with latitude, indicating that Rapoport's rule does not apply to E Atlantic fishes. Rather, the latitudinal patterns in species richness, size, and distributional range of benthic Atlantic fish were depth-dependent, because species richness, average maximum size, and the average latitudinal range increased with depth, and declined with latitude. The importance of accounting for this depth-latitude covariation in the distribution of marine fish demonstrated here, together with recent evidence obtained for deep-sea benthic macrofauna, points to depth and latitude as the main factors in the distribution of marine animals.This manuscript was partially funded by a grant from the Spanish Interministerial Commission of Science and Technology (CICYT MAR-91-0860).Peer reviewe