An AFM Approach of RBC Micro and Nanoscale Topographic Features During Storage

Blood gamma irradiation is the only available method to prevent transfusion-associated graft versus host disease (TA-GVHD). However, when blood is irradiated, determine blood shelf time is crucial. Non-irradiated blood has a self-time from 21 to 35 days when is preserved with an anticoagulated solution and stored at 4°C. During their storage, red blood cells (RBC) undergo a series of biochemical, biomechanical and molecular changes involving what is known as storage lesion (SL). SL include loss of structural integrity of RBC, a decrease of 2,3-diphosphatidylglyceric acid levels, and an increase of both ion potassium concentration and hemoglobin (Hb). On the other hand, Atomic force Microscopy (AFM) represents a versatile tool for a nano-scale high-resolution topographic analysis in biological systems. In order to evaluate SL in irradiated and non-irradiated blood, RBC topography and morphometric parameters were obtained from an AFM XE-BIO system. Cell viability was followed using flow cytometry. Our results showed that early markers as nanoscale roughness, allow us to evaluate blood quality since another perspective

Winter Forage Yield of Signal Grass (\u3cem\u3eBrachiaria brizantha \u3c/em\u3e(Hochst. Ex. A. Rich) R.D. Webster) at Different Cutting Frequency and N Fertilization

Signal grass (Brachiaria brizantha (Hochst. Ex. A. Rich) R.D. Webster) is widely grown in sub-humid tropical environments. In winter, shorter photoperiod and lower rainfall and temperatures reduce grass forage yield. Cutting frequency and N fertilization might reduce this negative effect (Whitehead, 1995; Pereira et al., 2014). The objective of the study was to determine winter forage yield total and by components of Signal grass under different cutting frequency and N fertilization

Critical Nitrogen and Nutritive Index in Cultivars of Cenchrus purpureus (Schumach) Morrone

Objective: to determine and compare the parameters of the nitrogen curve and the nutritive nitrogen index of cultivars of C. purpureus with and without nitrogen fertilization. Design / methodology / approach: Five cultivars of C. purpureus were evaluated, with or without fertilization, the dose was 141-43-20. The grass was harvested every 21 days with production of dry matter (DM) and nitrogen (N). Growth rates and Critical Nitrogen (Nc) were obtained. The negative power equation was used with its parameters Nc and the Nitrogen Decrease Coefficient (Cd). The Nutritive Nitrogen Index (INN) was obtained with the Nc parameter of each curve, divided by the N content of the cultivar in each sampling. The data were analyzed with a completely randomized design in a factorial arrangement of 5 (cultivars) and 2 (with or without fertilization). Results: The results of the cultivars show a Nc of 2.6 and a Cd of 0.65 fertilized and 1.14 of Nc and 0.68 of Cd without fertilization, in turn all of them supplemented the amount of N for their soil growth. The INN for the fertilized cultivars was 0.8 with classification of excess and sufficient for the first 56 days. Limitations of the study / implications: The variables to determine the availability of water and the balance of N, soil-plant, would further expand the knowledge of these tools. Findings / conclusions: the parameters obtained such as Nc and INN help us to determine the nutritional status of forages.Objective: To determine and compare the parameters of the nitrogen curve and the nutritive nitrogen index of cultivars of Cenchrus purpureus with and without nitrogenous fertilization.Design/Methodology/Approach: Five cultivars of C. purpureus with or without fertilization were evaluated, and the dose was 141N-43P-20K. The grass was harvested every 21 d with production of dry matter (DM) and nitrogen (N). Growth rates and Critical Nitrogen (Nc) were obtained. The negative power equation with its parameters Nc and the Nitrogen Decrease Coefficient (Cd) were used. The Nutritive Nitrogen Index (NNI) was obtained with the Nc parameter of each curve, divided by the N content of the cultivar in each sampling. The data were analyzed with a completely randomized design in a factorial arrangement of five (cultivars) and two levels of fertilization. Results: Nc of 2.6 and a Cd of 0.65 fertilized, and 1.14 of Nc and 0.68 of Cd without fertilization, and in turn all of them supplemented the amount of N for their growth from soil. The NNI was 0.8 for the fertilized cultivars, with classification of excess and sufficient for the first 56 d. Study Limitations/Implications: Variables to determine the availability of water and the balance of soil-plant N could further expand the knowledge of these tools. Findings/Conclusions: Parameters obtained such as Nc and NNI help to determine nutritional status of the fodders

Strategies for the coexistence of I.E.D. Rural Palermo

La presente investigación tuvo como objetivo reconstruir la historia del corregimiento de Tasajera para la preservación de la identidad cultural de los estudiantes. La investigación es de tipo cualitativa, la población participante son los estudiantes de cuarto grado de la Institución Educativa Rural Palermo. Como técnica de recolección de información se utilizó la entrevista y como instrumento se aplicó la historia de vida. Como conclusiones es importante inferir que a interior de la escuela existe poca tolerancia entre los estudiantes por lo que el reto, para la Institución Educativa es realizar estrategias que fortalezcan una sana convivencia de la mano con la libertad y la forma de pensar de la comunidad, debido a que existe una analogía con la educación, por lo que éstos factores benefician a las personas en situaciones problemáticas frecuentes en su cotidianidad.The objective of the present investigation was to reconstruct the history of the corregimiento of Tasajera for the preservation of the cultural identity of the students. The research is qualitative, the participating population is the fourth grade students of the Palermo Rural Educational Institution. As an information gathering technique, the interview was used and the history of life was applied as an instrument. As conclusions it is important to infer that within the school there is little tolerance among students so the challenge, for the Educational Institution is to implement strategies that strengthen a healthy coexistence hand in hand with democracy and citizenship training since it has a close relationship with education and therefore these aspects favor individuals so that they have and can carry in the situations that are presented to them on a daily basis

Applications of Wine Pomace in the Food Industry: Approaches and Functions

Winemaking generates large amounts ofwine pomace, also called grape pomace. This by-product has attracted the attention of food scientists and the food industry, due to its high content in nutrients and bioactive compounds. This review mainly focuses on the different published approaches to the use of wine pomace and its functions in the food industry. Traditionally, wine pomace has been used to obtain wine alcohol, food colorings, and grape seed oil. More recently, research has focused in the production of other value-added products, such as extracts of bioactive compounds, mainly phenols, recovery of tartaric acid, and the making of flours. The most common functions associated with wine pomace products are their use as antioxidants, followed by their use as fortifying, coloring, and antimicrobial agents. These products have mainly been applied to the preparation of meat and fish products and to, a lesser extent, cereal products.Autonomous Government of Castilla y León, Spain, through the research project BU282U13

Data for: Identification of inhalable rutile and polycyclic aromatic hydrocarbons (PAHs) nanoparticles in the atmospheric dust.

Analysis of road dust suggests that high rutile concentrations are deposited on the street and might be circulated in the air by wind, vehicle and pedestrian traffic. Atmospheric samples collected at pedestrian level are composed of a heterogeneous sample of micro and nanoparticles with a higher size range from 40 nm to 60 nm

Data for: Identification of inhalable rutile and polycyclic aromatic hydrocarbons (PAHs) nanoparticles in the atmospheric dust.

Analysis of road dust suggests that high rutile concentrations are deposited on the street and might be circulated in the air by wind, vehicle and pedestrian traffic. Atmospheric samples collected at pedestrian level are composed of a heterogeneous sample of micro and nanoparticles with a higher size range from 40 nm to 60 nm.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

On the forbidden graphene’s ZO (out-of-plane optic) phononic band-analog vibrational modes in fullerenes

The study of nanostructures’ vibrational properties is at the core of nanoscience research. They are known to represent a fingerprint of the system as well as to hint the underlying nature of chemical bonds. In this work, we focus on addressing how the vibrational density of states (VDOS) of the carbon fullerene family (Cn: n = 20 → 720 atoms) evolves from the molecular to the bulk material (graphene) behavior using density functional theory. We find that the fullerene’s VDOS smoothly converges to the graphene characteristic line-shape, with the only noticeable discrepancy in the frequency range of the out-of-plane optic (ZO) phonon band. From a comparison of both systems we obtain as main results that: (1) The pentagonal faces in the fullerenes impede the existence of the analog of the high frequency graphene’s ZO phonons, (2) which in the context of phonons could be interpreted as a compression (by 43%) of the ZO phonon band by decreasing its maximum allowed radial-optic vibration frequency. And 3) as a result, the deviation of fullerene’s VDOS relative to graphene may hold important thermodynamical implications, such as larger heat capacities compared to graphene at room-temperature. These results provide insights that can be extrapolated to other nanostructures containing pentagonal rings or pentagonal defects.TU Berlin, Open-Access-Mittel – 202

Size evolution of characteristic acoustic oscillations of fullerenes and its connection to continuum elasticity theory

We performed a theoretical investigation, based on density functional theory, on the vibrational properties of fullerenes and its dependence with size ($$20\rightarrow 720$$ atoms). Characteristic acoustic oscillations like the breathing (BM) and quadrupolar (QM) modes were located using the calculated vibrational density of states. In particular, it was obtained that the acoustic gap (lowest frequency value) corresponds to the QM five-fold degenerate frequency, as expected in cage-like quasi-spherical nanostructures. The main finding indicates a linear dependence for the vibrational periods of the BM and QM with the fullerenes size. The results obtained for the BM are consistent with a continuum elastic theory approach to describe the acoustic oscillations of a cage-like structure. Moreover, this behavior is also similar to that one found in metal nanoparticles with size in the range of 0.5–4 nm, indicating that the covalent nature of the bonding in fullerenes does not induce anomalous effects in their acoustic oscillations

Reliability and performances of real-time time-dependent auxiliary density functional theory

International audienceWe recently adapted the Auxiliary DFT framework as implemented in deMon2k to the simulation of time-dependent problems via the Runge and Gross equations. Our implementation of the so-called Real-Time-Time-Dependent ADFT (RT-TD-ADFT) fully benefits from the algorithms available in deMon2k to carry out variational density fitting, notably the MINRES algorithm recently proposed for self-consistent-field calculations. We test here MINRES for the first time in the context of RT-TD-ADFT. We report extensive benchmarks calculations to assess the reliability of the ADFT framework. These encompass the construction of absorption spectra in the gas phase and in solvent, the calculation of electronic stopping power curves, the irradiation of zeolites by swift ions and the investigation of charge migrations with attosecond time resolution. All our results are very encouraging. We show that even small auxiliary basis sets are sufficient to obtain results almost undisguisable from those obtained with large and flexible auxiliary bases. Overall, we establish the reliability of RT-TD-ADFT to simulate electronics dynamics in large or very large molecular systems