Confronting conflicts:history teachers’ reactions on spontaneous controversial remarks

Sometimes, things don't go to plan. Current events come into the classroom, especially the history classroom. How should students' responses to current affairs be dealt with there? How should students' desire to voice their opinions be handled if their opinion is unpopular. What if the student is simply wrong? How far can moral relativism be acknowledged, explored and scrutinised in the history classroom, when the topic under discussion is controversial and urgent? Working in the Netherlands and Belgium, Wansink, Patist, Zuiker, Savenije and Janssenswillen have developed and refined ways of doing this. In this article they provide an overview of researchers' thinking on the issue, and clear strategies and guidelines for what a history teacher might do to ensure that any unplanned discussion is, at least, respectful, engaging and rigorous

Pituitary stem cells produce paracrine WNT signals to control the expansion of their descendant progenitor cells

In response to physiological demand, the pituitary gland generates new hormonesecreting cells from committed progenitor cells throughout life. It remains unclear to what extent pituitary stem cells (PSCs), which uniquely express SOX2, contribute to pituitary growth and renewal. Moreover, neither the signals that drive proliferation nor their sources have been elucidated. We have used genetic approaches in the mouse, showing that the WNT pathway is essential for proliferation of all lineages in the gland. We reveal that SOX2+ stem cells are a key source of WNT ligands. By blocking secretion of WNTs from SOX2+ PSCs in vivo, we demonstrate that proliferation of neighbouring committed progenitor cells declines, demonstrating that progenitor multiplication depends on the paracrine WNT secretion from SOX2+ PSCs. Our results indicate that stem cells can hold additional roles in tissue expansion and homeostasis, acting as paracrine signalling centres to coordinate the proliferation of neighbouring cells

Draught beer hygiene: cleaning of dispense tap nozzles

Draught beer quality can be compromised by the growth of spoilage microorganisms. Whilst best practice for assuring dispense hygiene is broadly recognized, it is not always fully or regularly implemented. In some markets, tap nozzles are removed and stored overnight at room temperature in carbonated (soda) water. The next morning they are returned (sometimes after rinsing) to the dispense tap. The effectiveness of this approach is compared with soaking in diluted line-cleaning solution (UK best practice)or a solution containing hypochlorous acid (commercial sanitizing tablets). Two novel approaches – ozonated water and use of ultrasonics – were also evaluated. Bioluminescence analysis of microbial attachment to the inner surfaces of nozzles showed that soaking in carbonated water resulted in gross contamination. Sanitizing tablets achieved ‘commercial sterility’ and a 4-log reduction in bioluminescence compared with carbonated water. The efficacy of hypochlorous acid was confirmed by incubating cleaned nozzles in fresh beer without any increase in turbidity. Diluted line-cleaning solution was less effective and achieved a 2-log reduction. Ultrasonics reduced microbial attachment but effectiveness was aligned to increasing process time. Soaking in ozonated water was without antimicrobial impact. This work has shown carbonated water to be ineffective in cleaning microbiologically contaminated nozzles. This is a concern as these microorganisms derive from the dispense line, the environment and likely human interaction. To minimize the risks of transfer to dispensed product or back-contaminating the dispense line, soaking draught beer nozzles in an effective sanitizing solution is strongly recommended

Ultrasound-assisted extraction of polyphenols from potato peels: profiling and kinetic modelling

peer-reviewedUltrasound‐assisted extraction (UAE) at 33 and 42 kHz has been investigated in the extraction of polyphenols from peels of two potato varieties, cream‐skinned Lady Claire (LC) and pink‐skinned Lady Rosetta (LR), commonly used in snack food production. Extraction efficacy between the UAE‐untreated (control) and the UAE‐treated extracts was assessed on the total phenolic content and antioxidant capacities (DPPH and FRAP). Application of UAE showed significantly higher recovery of phenolic compounds compared to solid–liquid extraction process alone. Lower ultrasonic frequency (33 kHz) was more effective in recovering polyphenols compared to 42 kHz ultrasonic treatment. The liquid chromatography‐tandem mass spectrometry revealed that chlorogenic acid and caffeic acid were the most prevalent phenolics in LR peels, whereas caffeic acid was dominant in LC peels. Peleg's equation showed a good correlation (R2 > 0.92) between the experimental values and the predicted values on the kinetics of UAE of phenolic compounds.The authors acknowledge financial support from the ‘NovTechIng’ project funded under the Food Institutional Research Measure (Project No. FIRM/11/F/050) by the Irish Department of Agriculture, Food and Marine

An in vitro spinal cord injury model to screen neuroregenerative materials

Implantable 'structural bridges' based on nanofabricated polymer scaffolds have great promise to aid spinal cord regeneration. Their development (optimal formulations, surface functionalizations, safety, topographical influences and degradation profiles) is heavily reliant on live animal injury models. These have several disadvantages including invasive surgical procedures, ethical issues, high animal usage, technical complexity and expense. In vitro 3-D organotypic slice arrays could offer a solution to overcome these challenges, but their utility for nanomaterials testing is undetermined. We have developed an in vitro model of spinal cord injury that replicates stereotypical cellular responses to neurological injury in vivo, viz. reactive gliosis, microglial infiltration and limited nerve fibre outgrowth. We describe a facile method to safely incorporate aligned, poly-lactic acid nanofibre meshes (±poly-lysine + laminin coating) within injury sites using a lightweight construct. Patterns of nanotopography induced outgrowth/alignment of astrocytes and neurons in the in vitro model were strikingly similar to that induced by comparable materials in related studies in vivo. This highlights the value of our model in providing biologically-relevant readouts of the regeneration-promoting capacity of synthetic bridges within the complex environment of spinal cord lesions. Our approach can serve as a prototype to develop versatile bio-screening systems to identify materials/combinatorial strategies for regenerative medicine, whilst reducing live animal experimentation.EPSRC Doctoral Training Centre in regenerative medicine (EP/F500491/1

Surfactants-based remediation as an effective approach for removal of environmental pollutants—A review

Deterioration of environmental quality and equilibrium by rampant industrial expansion, accelerated urbanization and unchecked population growth has become a high-priority concern. The release of an alarming number of toxic polluting agents such as volatile organic compounds, dyes, heavy metals, pharmaceuticals, pesticides, industrial wastes, and personal care products due to natural or anthropogenic activities pose direct adverse effects on human health and living entities. This issue is inescapably increased because of the lack of efficient technologies for the proper disposal, management, and recycling of waste. It is of paramount importance to track alternative solutions to address these pollution problems for an eco-sustainable environment. Conventional remediation techniques are either inefficient, cumbersome or restricted due to certain techno-economic limitations. Environmental compatibility and high pollutant-removal efficacy make surfactants valuable for removal of organic pollutants and toxic heavy metal ions from different mediums. In this review, we present recent and up-to-date information on micelles/surfactants-assisted abatement of a vast number of toxic agents of emerging concern from water/wastewater including volatile organic compounds, personal care products, pharmaceutically active residues, toxic metals, dye pollutants, pesticides, and petroleum hydrocarbons. Based on the literature survey, it can be concluded that micelles-assisted water and soil treatment technology can have a better future on large-scale decontamination of wastewater. Though bio-surfactants are environmentally friendlier matrices and have successfully been employed for environmental decontamination; their large-scale applicability is challenging owing to high costs. Additional research efforts on the development and employment of novel bio-surfactants might render wastewater treatment technology greener, smarter and economical