Estudio de factibilidad para la realización de hamburguesas a base de gluten de trigo en la localidad de Kennedy

Tablas, graficas, mapas, imágenesPara el desarrollo del proyecto se estudia la factibilidad de crear y ejecutar una empresa procesadora de hamburguesas a base de gluten de trigo, estas se caracterizan por ser un producto innovador dirigido a personas de diversas clases sociales, que pueden acceder a este novedoso producto por su bajo costo. Por esta razón se realiza un producto de buena calidad que presentan propiedades físicas y químicas muy particulares con relación a otros productos sustitutos, ofreciendo una nutrición rica y balanceada; estas cualidades hacen que el producto sea necesario para el consumidor, creando una demanda insatisfecha en el mercado; la cual se verifico mediante encuestas realizadas a los habitantes de esta zona y por consiguiente genera un nivel de oferta bajo del producto ya que la competencia es mínima. Teniendo en cuenta lo mencionado anteriormente se ha elegido desarrollar el proyecto en la localidad de Kennedy, ya que este sector cuenta con los servicios públicos necesarios y la planta procesadora posee buenas condiciones de infraestructura, además porque no existen normas o restricciones que impidan la ejecución del proyecto. Se establece por medio del estudio financiero que el procesamiento de las hamburguesas a base de gluten de trigo es viable, y que de igual forma la relación costo beneficio indica que por cada peso invertido en el desarrollo del proyecto se obtiene una ganancia adicional. Se determina que el presente proyecto se ubica en el sector secundario, en el subsector de industrias alimenticias diversas, con la actividad económica de fabricación de hamburguesas a base de gluten de trigo.No Abstrac

On the nature of the EXor accretion events: an unfrequent manifestation of a common phenomenology ?

We present the results of a comparison between classical and newly identified EXor based on literature data and aimed at recognizing possible differences or similarities of both categories. Optical and near-IR two-color diagrams, modalities of fluctuations, and derived values of the mass accretion rates are indicative of strong similarities between the two samples. We demonstrate how the difference between the outburst and the quiescence spectral energy distribution of all the EXor can be well fitted with a single blackbody, as if an additional thermal component appears during the outbursting phase. Temperatures of this additional component span between 1000 and 4500 K, while the radii of the emitting regions (assumed to be a uniform disk) span between 0.01 and 0.1 AU, sizes typical of the inner portions of the circumstellar disk. Spots persisting up to 50% of the outburst duration, not exceeding the 10% of the stellar surface, and with temperatures compatible with the EXor mass accretion rates, are able to account for both the appearance of the additional thermal component and the dust sublimation in the inner structures of the disk. We also compare the EXor events with the most significant color and magnitude fluctuations of active T Tauri stars finding that (i} burst accretion phenomena should also be important for this latter class; (ii} EXor events could be more frequent then those accidentally discovered. Remarkable is the case of the source V2493 Cyg, a T Tauri star recently identified as a strong outbursting object: new optical and near-IR photometric and spectroscopic data are presented trying to clarify its EXor or FUor nature.Comment: Accepted for publication in Ap

Myeloid C/EBPβ deficiency reshapes microglial gene expression and is protective in experimental autoimmune encephalomyelitis

Background CCAAT/enhancer binding protein β (C/EBPβ) is a transcription factor that regulates the expression of important pro-inflammatory genes in microglia. Mice deficient for C/EBPβ show protection against excitotoxic and ischemic CNS damage, but the involvement in this neuroprotective effect of the various C/EBPβ-expressing cell types is not solved. Since C/EBPβ-deficient microglia show attenuated neurotoxicity in culture, we hypothesized that specific C/EBPβ deficiency in microglia could be neuroprotective in vivo. In this study, we have tested this hypothesis by generating mice with myeloid C/EBPβ deficiency. Methods Mice with myeloid C/EBPβ deficiency were generated by crossing LysMCre and C/EBPβfl/fl mice. Primary microglial cultures from C/EBPβfl/fl and LysMCre-C/EBPβfl/fl mice were treated with lipopolysaccharide ± interferon γ (IFNγ) for 6 h, and gene expression was analyzed by RNA sequencing. Gene expression and C/EBPβ deletion were analyzed in vivo in microglia isolated from the brains of C/EBPβfl/fl and LysMCre-C/EBPβfl/fl mice treated systemically with lipolysaccharide or vehicle. Mice of LysMCre-C/EBPβfl/fl or control genotypes were subjected to experimental autoimmune encephalitis and analyzed for clinical signs for 52 days. One- or two-way ANOVA or Kruskal-Wallis with their appropriate post hoc tests were used. Results LysMCre-C/EBPβfl/fl mice showed an efficiency of C/EBPβ deletion in microglia of 100 and 90% in vitro and in vivo, respectively. These mice were devoid of female infertility, perinatal mortality and reduced lifespan that are associated to full C/EBPβ deficiency. Transcriptomic analysis of C/EBPβ-deficient primary microglia revealed C/EBPβ-dependent expression of 1068 genes, significantly enriched in inflammatory and innate immune responses GO terms. In vivo, microglial expression of the pro-inflammatory genes Cybb, Ptges, Il23a, Tnf and Csf3 induced by systemic lipopolysaccharide injection was also blunted by C/EBPβ deletion. CNS expression of C/EBPβ was upregulated in experimental autoimmune encephalitis and in multiple sclerosis samples. Finally, LysMCre-C/EBPβfl/fl mice showed robust attenuation of clinical signs in experimental autoimmune encephalitis. Conclusion This study provides new data that support a central role for C/EBPβ in the biology of activated microglia, and it offers proof of concept for the therapeutic potential of microglial C/EBPβ inhibition in multiple sclerosis

Rate and determinants of treatment response to different antiretroviral combination strategies in subjects presenting at HIV-1 diagnosis with advanced disease

<p>Abstract</p> <p>Background</p> <p>The optimal therapeutic strategies for patients presenting with advanced disease at HIV-1 diagnosis are as yet incompletely defined.</p> <p>Methods</p> <p>All patients presenting at two outpatient clinics in 2000-2009 with an AIDS-defining clinical condition or a CD4+ T cell count < 200/μL at HIV-1 diagnosis were analyzed for the presence of combined immunovirological response, defined by the concomitant presence of an absolute number of CD4+ T cells > 200 cells/μL and a plasma HIV-1 RNA copy number < 50/mL after 12 months of HAART.</p> <p>Results</p> <p>Among 102 evaluable patients, first-line regimens were protease inhibitors [PI]-based in 78 cases (77%) and efavirenz-based in 24 cases (23%). The overall response rate was 65% (95% CI: 55-74), with no differences by gender, age, nationality, route of transmission, hepatitis virus coinfections, presence of AIDS-defining clinical events, baseline HIV-1 viral load, or type of regimen (response rates with PI-based and efavirenz-based therapy: 63% and 71%, respectively, p = 0.474). Response rate was significantly better with higher baseline CD4+ T cell counts (78% with CD4+ ≥ 100/μL, compared to 50% with CD4+ < 100/μL; odds ratio: 3.5; 95% CI: 1.49-8.23, p = 0.003). Median time on first-line antiretroviral therapy was 24 months (interquartile range: 12-48). Switch to a second line treatment occurred in 57% of patients, mainly for simplification (57%), and was significantly more common with PI-based regimens [adjusted hazard ratios (AHR) with respect to efavirenz-based regimens: 3.88 for unboosted PIs (95% CI: 1.40-10.7, p = 0.009) and 4.21 for ritonavir-boosted PI (95%CI 1.7-10.4, p = 0.002)] and in older subjects (≥ 50 years) (AHR: 1.83; 95% CI: 1.02-3.31, p = 0.044). Overall mortality was low (3% after a median follow up of 48 months).</p> <p>Conclusions</p> <p>Our data indicate that a favorable immunovirological response is possible in the majority of naive patients presenting at HIV-1 diagnosis with AIDS or low CD4+ T cell counts, and confirm that starting HAART with a more compromised immune system may be associated with a delayed and sometimes partial immune recovery. Simpler regimens may be preferable in this particular population.</p

Near Infrared Spectroscopic Monitoring of EXor variables: First Results

We present low resolution (R approximately 250) spectroscopy in the near-IR (0.8 to 2.5um) of the EXor variables. These are the initial results (obtained during the period 2007-2008) from a long term photometric and spectroscopic program aimed to study the variability in the accretion processes of pre-Main Sequence (PMS) stars, by correlating the continuum fluctuations with the spectroscopical properties. Eight sources have been observed in different epochs, for a total of 25 acquired spectra. EXor spectra show a wide variety of emission features dominated by HI recombination (Paschen and Brackett series). We have investigated whether line and continuum variability could be due to a variable extinction, but such hypothesis is applicable only to the peculiar source PV Cep. By comparing the observed spectra with a wind model, mass loss rates in the range (2-10)x10^(-8) M_sun} yr^(-1) are derived, along with other wind parameters. Consistent results are also obtained by assuming that HI lines are due to accretion. CO overtone is also detected in the majority of the sources both in absorption and in emission. It appears to come from regions more compact than winds, likely the stellar photosphere (when in absorption) and the circumstellar disk (when in emission). NaI and CaI IR lines behave as the CO does, thus they are thought to arise in the same locations. For some targets multiple spectra correspond to different activity stages of the source. Those exhibiting the largest continuum variation at 2um (DeltaK > 1 mag) present a significant line flux fading during the continuum declining phases. In particular, CO absorption (emission) appears associated to inactive (active) stages, respectively.Comment: To be published in Ap

AGILE detection of extreme gamma-ray activity from the blazar PKS 1510-089 during March 2009. Multifrequency analysis

We report on the extreme gamma-ray activity from the FSRQ PKS 1510-089 observed by AGILE in March 2009. In the same period a radio-to-optical monitoring of the source was provided by the GASP-WEBT and REM. Moreover, several Swift ToO observations were triggered, adding important information on the source behaviour from optical/UV to hard X-rays. We paid particular attention to the calibration of the Swift/UVOT data to make it suitable to the blazars spectra. Simultaneous observations from radio to gamma rays allowed us to study in detail the correlation among the emission variability at different frequencies and to investigate the mechanisms at work. In the period 9-30 March 2009, AGILE detected an average gamma-ray flux of (311+/-21)x10^-8 ph cm^-2 s^-1 for E>100 MeV, and a peak level of (702+/-131)x10^-8 ph cm^-2 s^-1 on daily integration. The gamma-ray activity occurred during a period of increasing activity from near-IR to UV, with a flaring episode detected on 26-27 March 2009, suggesting that a single mechanism is responsible for the flux enhancement observed from near-IR to UV. By contrast, Swift/XRT observations seem to show no clear correlation of the X-ray fluxes with the optical and gamma-ray ones. However, the X-ray observations show a harder photon index (1.3-1.6) with respect to most FSRQs and a hint of harder-when-brighter behaviour, indicating the possible presence of a second emission component at soft X-ray energies. Moreover, the broad band spectrum from radio-to-UV confirmed the evidence of thermal features in the optical/UV spectrum of PKS 1510-089 also during high gamma-ray state. On the other hand, during 25-26 March 2009 a flat spectrum in the optical/UV energy band was observed, suggesting an important contribution of the synchrotron emission in this part of the spectrum during the brightest gamma-ray flare, therefore a significant shift of the synchrotron peak.Comment: 13 pages, 7 figures, 3 tables. Accepted for publication in Astronomy and Astrophysic

Hydrophilic antioxidant compounds in orange juice from different fruit cultivars: Composition and antioxidant activity evaluated by chemical and cellular based (Saccharomyces cerevisiae) assays

Antioxidant capacity was evaluated by a cellular model (Saccharomyces cerevisiae) and chemical methods (FRAP, TEAC and total phenols by Folin-Ciocalteu assay) in the hydrophilic fraction (phenolic compounds and ascorbic acid) of orange juices (OJs) from six varieties (Midknight, Delta Seedless, Rohde Red, Seedless, Early and clone Sambiasi), harvested in two seasons. The contents of phenolic compounds and ascorbic acid analyzed, respectively, by UPLC and HPLC were 370.04 76.97 mg/L and 52.05 6.69 mg/100 mL. Variety and season significantly influenced (p < 0.05) composition and antioxidant capacity. TEAC and FRAP values correlated well with individual hydrophilic compounds (R2 > 0.991) but no correlation with cellular assay was observed. An increase in survival rates between 23% and 38% was obtained, excepting for two varieties that showed no activity (Rohde Red and Seedless). Narirutin, naringin-d, ferulic acid-d2, didymin, neoeriocitrin and sinapic acid hexose and caffeic acid-d1 were the phenolic compounds which contributed to survival rates (R2 = 0.979, p < 0.01

The WEBT campaign on the BL Lac object PG 1553+113 in 2013. An analysis of the enigmatic synchrotron emission

A multifrequency campaign on the BL Lac object PG 1553+113 was organized by the Whole Earth Blazar Telescope (WEBT) in 2013 April-August, involving 19 optical, two near-IR, and three radio telescopes. The aim was to study the source behaviour at low energies during and around the high-energy observations by the Major Atmospheric Gamma-ray Imaging Cherenkov telescopes in April-July. We also analyse the UV and X-ray data acquired by the Swift and XMM-Newton satellites in the same period. The WEBT and satellite observations allow us to detail the synchrotron emission bump in the source spectral energy distribution (SED). In the optical, we found a general bluer-when-brighter trend. The X-ray spectrum remained stable during 2013, but a comparison with previous observations suggests that it becomes harder when the X-ray flux increases. The long XMM-Newton exposure reveals a curved X-ray spectrum. In the SED, the XMM-Newton data show a hard near-UV spectrum, while Swift data display a softer shape that is confirmed by previous Hubble Space Telescope/Cosmic Origins Spectrograph and International Ultraviolet Explorer observations. Polynomial fits to the optical-X-ray SED show that the synchrotron peak likely lies in the 4-30eV energy range, with a general shift towards higher frequencies for increasing X-ray brightness. However, the UV and X-ray spectra do not connect smoothly. Possible interpretations include: (i) orientation effects, (ii) additional absorption, (iii) multiple emission components, and (iv) a peculiar energy distribution of relativistic electrons. We discuss the first possibility in terms of an inhomogeneous helical jet mode

Multiwavelength behaviour of the blazar 3C 279: decade-long study from γ-ray to radio

We report the results of decade-long (2008–2018) γ-ray to 1 GHz radio monitoring of the blazar 3C 279, including GASP/WEBT, Fermi and Swift data, as well as polarimetric and spectroscopic data. The X-ray and γ-ray light curves correlate well, with no delay ≳3 h, implying general cospatiality of the emission regions. The γ-ray–optical flux–flux relation changes with activity state, ranging from a linear to a more complex dependence. The behaviour of the Stokes parameters at optical and radio wavelengths, including 43 GHz Very Long Baseline Array images, supports either a predominantly helical magnetic field or motion of the radiating plasma along a spiral path. Apparent speeds of emission knots range from 10 to 37c, with the highest values requiring bulk Lorentz factors close to those needed to explain γ-ray variability on very short time-scales. The Mg II emission line flux in the ‘blue’ and ‘red’ wings correlates with the optical synchrotron continuum flux density, possibly providing a variable source of seed photons for inverse Compton scattering. In the radio bands, we find progressive delays of the most prominent light-curve maxima with decreasing frequency, as expected from the frequency dependence of the τ = 1 surface of synchrotron self-absorption. The global maximum in the 86 GHz light curve becomes less prominent at lower frequencies, while a local maximum, appearing in 2014, strengthens toward decreasing frequencies, becoming pronounced at ∼5 GHz. These tendencies suggest different Doppler boosting of stratified radio-emitting zones in the jet