Occurrence, prevalence and molecular characterization of Norileca indica (Milne Edwards, 1840) (Isopoda: Cymothoidae) on bigeyescad Selar crumenophthalmus (Bloch) from Andaman Islands India

452-456The present investigation reports the occurrence and prevalence of the parasitic cymothoid, Norileca indica infesting bigeyescad, Selar crumenophthalmus from the Andaman Islands. Damage in the form of erosion of gill filament and necrosis in the gill chamber of the host fish, with a prevalence of 26.08% and a mean intensity of 1.5 is recorded. Molecular characterization of the mitochondrial COI and 28S rDNA genes is done to establish its phylogenetic status with its known congener. The present report is the first record of N. indica infesting S. crumenophthalmus from the Andaman Islands

Wsn integrated cloud computing for n-care system (ncs) using middleware services

AbstractThe number of wireless devices with powerful sensing capabilities is constantly growing. A mobile phone is an example of a device that is packed with several powerful sensors. Cloud computing is another area that been in focus over the last decade. Cloud computing can be defined as an architectural abstraction that provides scalability and reliability based on requirement. The challenge lies in the fact that sensors for different purposes are heterogeneous in nature. We propose a framework called the N-Care System that utilizes heterogeneous wireless networks to collect data, cloud services to provide additional computational capabilities and provides information for different types of end users. A wireless sensor network consisting of sensors that possess both sensing and transmitting capabilities forms a communication back-bone that can capture a wide variety of data. Multiple sensors are grouped in to a cluster that consists of an internet capable computing device called cluster head that collects data from the constituent sensor nodes and pushes it in to a cloud based database. End users can log in and access data from sensors that fall under the user’s domain

Clinical Asssessment of Sensorineural Hearing Loss among Diabetes Mellitus Patients

Background: Hearing impairment is known to hamper the quality of life among patients, especially among diabetics due to the association of neuropathy with diabetes. However, the prevalence and degree of the SNHL depends upon different factors, such as age, gender, disease duration of DM, family history and glycemic status of the patients. Therefore, this study aimed to assess the association of SNHL with DM duration and familial DM and gender preponderance among SNHL–DM patients. Methods: Total 140 patients with DM were assessed for hearing impairment using Rinne, Weber and Absolute Bone Conduction Tests along with pure tone audiometry. Patients’ glycaemic status was determined by estimating fasting blood glucose (FBG) and post prandial blood glucose (PPBG) levels. Independent t-test, chi-square, ANOVA and Pearson’s correlation tests along with linear regression model were used to find association and correlation using R software. Results: Out of 140 patients, 60 were suffering from SNHL and majority were suffering from bilateral minimal hearing loss. SNHL was significantly associated with family history, age, duration of DM, FBG and PPBG levels were(Pvalues:1.79E08, 4.41E-06 and 0.02), however, significant correlated with duration of DM, FBS and PPBG level (r value:0.14–0.41).Furthermore, significant SNHL at 500 and 8000Hz was observed in the present study (Pvalue:0.002). Conclusion: A conclusive proof was drawn that family history of DM serve as a valuable variable in assessing the SNHL among DM patients

A Comparative Study of the Effects of Anterior Nasal Packing versus Trans-Septal Suturing in Post-Septoplasty Patients

Introduction  Following  septoplasty,  the  usual  norm  is  to  pack  the  nose  using polyvinyl alcohol or  white petroleum  jelly impregnated gauze. But  due  to  side  effects  like  pain,  respiratory  problems, headache  or  pain  during  pack  removal,  trans-septal  suturing  is  recommended  as  it  provides  stability  and  prevents  septal  hematoma  formation  without  causing  the  above mentioned  complaints.  This  study  aims  to  conduct  an  in-depth  comparison  of  the  outcomes  of  the  two  aforementioned  methods  in  an  Indian  population. Materials and Methods  A  prospective  randomized  comparative  study  was  done  for  30  patients  between  18-50  years  undergoing  septoplasty,  while  excluding  those  requiring  additional  surgical  interventions.  Trans-septal  suturing  was  done  for  15  patients  and  polyvinyl alcohol  nasal  packing  for  the  rest.  Visual  Analogue  Scale  was  used  to  record  discomfort  levels  1  day  post-operatively.  A  vast  multitude  of  symptoms  were  assessed  during  regular  visits  for  3  months  after  surgery.  Results Post-operatively,  nasal  pain,  headache,  dyspnoea,  sleep  disturbance,  post-nasal  drip,  crusting,  and  epiphora  were  found  to  be   reduced  in  patients  who  underwent  trans-septal  suturing  when  compared  with  packing,  deeming  it  to  be  a  superior  choice  of  method.  However,  nasal  bleeding  was  comparable  in  both  groups. Conclusion Trans-septal  suturing  reduces  pack  related  problems  and  post-operative  complications,  without  significantly  increasing  the  duration  of  surgery.  Hence,  we  recommend  the  practice  of  placing  sutures  to  positively  improve  patients’  quality  of  life  post-surgery

Monograph on quality standards of Viscum angulatum B. Heyne ex DC.

The genus Viscum (Viscaceae) comprise of several species of hemi-parasitic plants with medicinal properties. Commonly known as mistletoes, these plants grow on other trees with the partial parasitic association. Viscum angulatum B.Heyne ex DC. is one such less explored leafless mistletoe of Asian countries with medicinal claims. In Ayurveda (Bandaka) and Siddha (Pulluri/Pulluruvi), many botanicals from mistletoe families have been attributed with medicinal properties. The objective of the current study is to develop a monograph on quality standards of V. angulatum occurring in high altitude hills of Western Ghats. Aerial parts of V. angulatum were collected and authenticated and preserved in FAA for microscopic studies and some quantity of the plant material was shade-dried and coarsely powdered. Successive extracts were subjected to chromatography and isolation - characterization of the major compounds. Leaf-less quadrangular stem was found to be a diagnostic macroscopic feature of this species. The preliminary phytochemical investigation of extracts showed presence of alkaloids, carbohydrates, coumarins, flavonoids, phytosterols, triterpenoids, saponins and tannins. HPTLC fingerprint of n-hexane and ethyl acetate extracts has been obtained for identification of extracts. The ethyl acetate extract yielded 10-hydroxyoleoside dimethyl ester which can be used as a marker compound for routine quality check of V. angulatum growing on Mussaenda hirsutissima. The set of data obtained in the present investigation can serve as a standard for the identification as well as further studies. These results on standards of this plant are the first report so far

Monograph on quality standards of Viscum angulatum B. Heyne ex DC.

320-332The genus Viscum (Viscaceae) comprise of several species of hemi-parasitic plants with medicinal properties. Commonly known as mistletoes, these plants grow on other trees with the partial parasitic association. Viscum angulatum B.Heyne ex DC. is one such less explored leafless mistletoe of Asian countries with medicinal claims. In Ayurveda (Bandaka) and Siddha (Pulluri/Pulluruvi), many botanicals from mistletoe families have been attributed with medicinal properties. The objective of the current study is to develop a monograph on quality standards of V. angulatum occurring in high altitude hills of Western Ghats. Aerial parts of V. angulatum were collected and authenticated and preserved in FAA for microscopic studies and some quantity of the plant material was shade-dried and coarsely powdered. Successive extracts were subjected to chromatography and isolation - characterization of the major compounds. Leaf-less quadrangular stem was found to be a diagnostic macroscopic feature of this species. The preliminary phytochemical investigation of extracts showed presence of alkaloids, carbohydrates, coumarins, flavonoids, phytosterols, triterpenoids, saponins and tannins. HPTLC fingerprint of n-hexane and ethyl acetate extracts has been obtained for identification of extracts. The ethyl acetate extract yielded 10-hydroxyoleoside dimethyl ester which can be used as a marker compound for routine quality check of V. angulatum growing on Mussaenda hirsutissima. The set of data obtained in the present investigation can serve as a standard for the identification as well as further studies. These results on standards of this plant are the first report so far

Rice-Magnaporthe transcriptomics reveals host defense activation induced by red seaweed-biostimulant in rice plants

Red seaweed extracts have been shown to trigger the biotic stress tolerance in several crops. However, reports on transcriptional modifications in plants treated with seaweed biostimulant are limited. To understand the specific response of rice to blast disease in seaweed-biostimulant-primed and non-primed plants, transcriptomics of a susceptible rice cultivar IR-64 was carried out at zero and 48 h post inoculation with Magnaporthe oryzae (strain MG-01). A total of 3498 differentially expressed genes (DEGs) were identified; 1116 DEGs were explicitly regulated in pathogen-inoculated treatments. Functional analysis showed that most DEGs were involved in metabolism, transport, signaling, and defense. In a glass house, artificial inoculation of MG-01 on seaweed-primed plants resulted in the restricted spread of the pathogen leading to the confined blast disease lesions, primarily attributed to reactive oxygen species (ROS) accumulation. The DEGs in the primed plants were defense-related transcription factors, kinases, pathogenesis-related genes, peroxidases, and growth-related genes. The beta-D-xylosidase, a putative gene that helps in secondary cell wall reinforcement, was downregulated in non-primed plants, whereas it upregulated in the primed plants indicating its role in the host defense. Additionally, Phenylalanine ammonia-lyase, pathogenesis-related Bet-v-I family protein, chalcone synthase, chitinases, WRKY, AP2/ERF, and MYB families were upregulated in seaweed and challenge inoculated rice plants. Thus, our study shows that priming rice plants with seaweed bio-stimulants resulted in the induction of the defense in rice against blast disease. This phenomenon is contributed to early protection through ROS, protein kinase, accumulation of secondary metabolites, and cell wall strengthening

Severe early onset preeclampsia: short and long term clinical, psychosocial and biochemical aspects

Preeclampsia is a pregnancy specific disorder commonly defined as de novo hypertension and proteinuria after 20 weeks gestational age. It occurs in approximately 3-5% of pregnancies and it is still a major cause of both foetal and maternal morbidity and mortality worldwide1. As extensive research has not yet elucidated the aetiology of preeclampsia, there are no rational preventive or therapeutic interventions available. The only rational treatment is delivery, which benefits the mother but is not in the interest of the foetus, if remote from term. Early onset preeclampsia (<32 weeks’ gestational age) occurs in less than 1% of pregnancies. It is, however often associated with maternal morbidity as the risk of progression to severe maternal disease is inversely related with gestational age at onset2. Resulting prematurity is therefore the main cause of neonatal mortality and morbidity in patients with severe preeclampsia3. Although the discussion is ongoing, perinatal survival is suggested to be increased in patients with preterm preeclampsia by expectant, non-interventional management. This temporising treatment option to lengthen pregnancy includes the use of antihypertensive medication to control hypertension, magnesium sulphate to prevent eclampsia and corticosteroids to enhance foetal lung maturity4. With optimal maternal haemodynamic status and reassuring foetal condition this results on average in an extension of 2 weeks. Prolongation of these pregnancies is a great challenge for clinicians to balance between potential maternal risks on one the eve hand and possible foetal benefits on the other. Clinical controversies regarding prolongation of preterm preeclamptic pregnancies still exist – also taking into account that preeclampsia is the leading cause of maternal mortality in the Netherlands5 - a debate which is even more pronounced in very preterm pregnancies with questionable foetal viability6-9. Do maternal risks of prolongation of these very early pregnancies outweigh the chances of neonatal survival? Counselling of women with very early onset preeclampsia not only comprises of knowledge of the outcome of those particular pregnancies, but also knowledge of outcomes of future pregnancies of these women is of major clinical importance. This thesis opens with a review of the literature on identifiable risk factors of preeclampsia

Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores

A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació