Thinking about the Future: Young People in Low-Income Families

This paper examines the orientations to the future of young people living in low-income families in the U.K. and Portugal following the 2008 Global Financial Crisis and the contexts in which they are socially reproduced. It is based on data from comparative research on families and food poverty, funded by the European Research Council. The study focused on parents and young people aged 11–16 living in low-income families in three European countries (the U.K., Portugal and Norway); only the U.K. and Portuguese data were analysed here. Given the study was concerned with the consequences of low income for food insecurity, we primarily sought to understand how young people manage in the present; however, the project also affords a theoretical and methodological opportunity to explore young people’s thoughts about the future as they begin to transition to adulthood. We found that, when asked about the future, young people responded in different ways: some said they did not think about the future; others mentioned their dreams, but considered them unrealisable. while others expressed hopes that were more concrete and achievable. Precarity constrained the control that young people and their families exercised over their lives. We argue that young people’s aspirations and time horizons are framed in relation to the present and the temporalities of the life course, the public discourses to which they are subjected and the limited access of their families to resources provided by the labour market and the state

School meals as a resource for low-income families in three European countries: a comparative case approach

In the context of successive global crises and rising household food insecurity in wealthy European countries there is renewed attention to the role of school meals as a welfare intervention. However, little is known about the extent to which school meals are a resource for low-income families living in different contexts. Drawing on a mixed methods study of food in low-income families in three European countries, this paper adopts a realist ontological stance and an embedded case study approach to address this question. The research concerns low-income families with children aged 11–15 years in the aftermath of the 2008 financial crisis in the UK, Portugal and Norway. Based on a comparative, multi-layered analysis of macro-, meso- and micro-level contexts, we argue that publicly funded, nutritious school meals protect children from the direct effects of poverty on their food security, whilst underfunded and weakly regulated school food provision compounds children’s experiences of disadvantage and exclusion. The paper concludes with recommendations for public policies that conceptualise school meals as a collective resource, like education, to which young people as bearers of the right to food are entitled.info:eu-repo/semantics/publishedVersio

Halpha Morphologies and Environmental Effects in Virgo Cluster Spiral Galaxies

We describe the various Halpha morphologies of Virgo Cluster and isolated spiral galaxies, and associate the Halpha morphologies with the types of environmental interactions which have altered the cluster galaxies. The spatial distributions of Halpha and R-band emission are used to divide the star formation morphologies of the 52 Virgo Cluster spirals into several categories: normal (37%), anemic (6%), enhanced (6%), and (spatially) truncated (52%). Truncated galaxies are further subdivided based on their inner star formation rates into truncated/normal (37%), truncated/compact (6%), truncated/anemic (8%), and truncated/enhanced (2%). The fraction of anemic galaxies is relatively small (6-13%) in both environments, suggesting that starvation is not a major factor in the reduced star formation rates of Virgo spirals. The majority of Virgo spiral galaxies have their Halpha disks truncated (52%), whereas truncated Halpha disks are rarer in isolated galaxies (12%). Most of the Halpha-truncated galaxies have relatively undisturbed stellar disks and normal-to-slightly enhanced inner disk star formation rates, suggesting that ICM-ISM stripping is the main mechanism causing the reduced star formation rates of Virgo spirals. In other galaxies, the Halpha morphologies are more consistent with a tidal origin or perhaps outer cluster HI accretion. These results indicate that most Virgo spiral galaxies experience ICM-ISM stripping, many experience significant tidal effects, and many experience both. (abridged).Comment: Accepted by Astrophysical Journal. 16 pages, 15 figures, including 9 in low-resolution jpg format. Higher resolution postscript versions of these figures are available from http://www1.union.edu/~koopmanr/radfig.htm

The malaria testing and treatment landscape in Kenya: results from a nationally representative survey among the public and private sector in 2016

Abstract Background Since 2004, Kenya’s national malaria treatment guidelines have stipulated artemisinin-based combination therapy (ACT) as first-line treatment for uncomplicated malaria, and since 2014, confirmatory diagnosis of malaria in all cases before treatment has been recommended. A number of strategies to support national guidelines have been implemented in the public and private sectors in recent years. A nationally-representative malaria outlet survey, implemented across four epidemiological zones, was conducted between June and August 2016 to provide practical evidence to inform strategies and policies in Kenya towards achieving national malaria control goals. Results A total of 17,852 outlets were screened and 2271 outlets were eligible and interviewed. 78.3% of all screened public health facilities stocked both malaria diagnostic testing and quality-assured ACT (QAACT). Sulfadoxine–pyrimethamine (SP) for intermittent preventive treatment in pregnancy was available in 70% of public health facilities in endemic areas where it is recommended for treatment. SP was rarely found in the public sector outside of the endemic areas (< 0.5%). The anti-malaria stocking private sector had lower levels of QAACT (46.7%) and malaria blood testing (20.8%) availability but accounted for majority of anti-malarial distribution (70.6% of the national market share). More than 40% of anti-malarials were distributed by unregistered pharmacies (37.3%) and general retailers (7.1%). QAACT accounted for 58.2% of the total anti-malarial market share, while market share for non-QAACT was 15.8% and for SP, 24.8%. In endemic areas, 74.9% of anti-malarials distributed were QAACT. Elsewhere, QAACT market share was 49.4% in the endemic-prone areas, 33.2% in seasonal-transmission areas and 37.9% in low-risk areas. Conclusion Although public sector availability of QAACT and malaria diagnosis is relatively high, there is a gap in availability of both testing and treatment that must be addressed. The private sector in Kenya, where the majority of anti-malarials are distributed, is also critical for achieving universal coverage with appropriate malaria case management. There is need for a renewed commitment and effective strategies to ensure access to affordable QAACT and confirmatory testing in the private sector, and should consider how to address malaria case management among informal providers responsible for a substantial proportion of the anti-malarial market share

A translational synthetic biology platform for rapid access to gram-scale quantities of novel drug-like molecules

Plants are an excellent source of drug leads. However availability is limited by access to source species, low abundance and recalcitrance to chemical synthesis. Although plant genomics is yielding a wealth of genes for natural product biosynthesis, the translation of this genetic information into small molecules for evaluation as drug leads represents a major bottleneck. For example, the yeast platform for artemisinic acid production is estimated to have taken >150 person years to develop. Here we demonstrate the power of plant transient transfection technology for rapid, scalable biosynthesis and isolation of triterpenes, one of the largest and most structurally diverse families of plant natural products. Using pathway engineering and improved agro-infiltration methodology we are able to generate gram-scale quantities of purified triterpene in just a few weeks. In contrast to heterologous expression in microbes, this system does not depend on re-engineering of the host. We next exploit agro-infection for quick and easy combinatorial biosynthesis without the need for generation of multi-gene constructs, so affording an easy entrée to suites of molecules, some new-to-nature, that are recalcitrant to chemical synthesis. We use this platform to purify a suite of bespoke triterpene analogs and demonstrate differences in anti-proliferative and anti-inflammatory activity in bioassays, providing proof of concept of this system for accessing and evaluating medicinally important bioactives. Together with new genome mining algorithms for plant pathway discovery and advances in plant synthetic biology, this advance provides new routes to synthesize and access previously inaccessible natural products and analogs and has the potential to reinvigorate drug discovery pipelines

The miR-155-PU.1 axis acts on Pax5 to enable efficient terminal B cell differentiation.

A single microRNA (miRNA) can regulate the expression of many genes, though the level of repression imparted on any given target is generally low. How then is the selective pressure for a single miRNA/target interaction maintained across long evolutionary distances? We addressed this problem by disrupting in vivo the interaction between miR-155 and PU.1 in mice. Remarkably, this interaction proved to be key to promoting optimal T cell-dependent B cell responses, a previously unrecognized role for PU.1. Mechanistically, miR-155 inhibits PU.1 expression, leading to Pax5 down-regulation and the initiation of the plasma cell differentiation pathway. Additional PU.1 targets include a network of genes whose products are involved in adhesion, with direct links to B-T cell interactions. We conclude that the evolutionary adaptive selection of the miR-155-PU.1 interaction is exercised through the effectiveness of terminal B cell differentiation

Conserved transcriptomic profiles underpin monogamy across vertebrates

Social monogamy, typically characterized by the formation of a pair bond, increased territorial defense, and often biparental care, has independently evolved multiple times in animals. Despite its independent origins, several homologous brain regions, neuropeptides and their receptors have been shown to play a conserved role in regulating social affiliation and parental care, but little is known is known about the neuromolecular mechanisms underlying monogamy on a genomic scale. Here, we compare neural transcriptomes of reproductive males in monogamous and nonmonogamous species pairs of Peromyscus mice, Microtus voles, parid songbirds, dendrobatid frogs, and Xenotilapia species of cichlid fishes. We find that while evolutionary divergence time between species or clades did not explain gene expression similarity, characteristics of mating system correlated with neural gene expression patterns and neural gene expression varies concordantly across vertebrates when species transition to monogamy. Our study provides evidence of a universal transcriptomic mechanism underlying the evolution of monogamy in vertebrates

Metformin is a metabolic modulator and radiosensitiser in rectal cancer

Resistance to neoadjuvant chemoradiation therapy, is a major challenge in the management of rectal cancer. Increasing evidence supports a role for altered energy metabolism in the resistance of tumours to anti-cancer therapy, suggesting that targeting tumour metabolism may have potential as a novel therapeutic strategy to boost treatment response. In this study, the impact of metformin on the radiosensitivity of colorectal cancer cells, and the potential mechanisms of action of metformin-mediated radiosensitisation were investigated. Metformin treatment was demonstrated to significantly radiosensitise both radiosensitive and radioresistant colorectal cancer cells in vitro. Transcriptomic and functional analysis demonstrated metformin-mediated alterations to energy metabolism, mitochondrial function, cell cycle distribution and progression, cell death and antioxidant levels in colorectal cancer cells. Using ex vivo models, metformin treatment significantly inhibited oxidative phosphorylation and glycolysis in treatment naïve rectal cancer biopsies, without affecting the real-time metabolic profile of non-cancer rectal tissue. Importantly, metformin treatment differentially altered the protein secretome of rectal cancer tissue when compared to non-cancer rectal tissue. Together these data highlight the potential utility of metformin as an anti-metabolic radiosensitiser in rectal cancer

Prevalence and correlates of alcohol dependence disorder among TB and HIV infected patients in Zambia.

OBJECTIVES: To determine the prevalence and correlates of alcohol dependence disorders in persons receiving treatment for HIV and Tuberculosis (TB) at 16 Primary Health Care centres (PHC) across Zambia. METHODS: 649 adult patients receiving treatment for HIV and/or TB at PHCs in Zambia (363 males, 286 females) were recruited between 1st December 2009 and 31st January 2010. Data on socio-demographic variables, clinical disease features (TB and HIV), and psychopathological status were collected. The Mini International Neuropsychiatric Interview (MINI) was used to diagnose alcohol dependence disorder. Correlates of alcohol dependence were analyzed for men only, due to low prevalence in women. Univariable and multivariable logistic regression models were used to estimate odds ratios (OR) and 95% confidence intervals (CI), using general estimating equations to allow for within-PHC clustering. RESULTS: The prevalence of alcohol dependence was 27.2% (95%CI: 17.7-39.5%) for men and 3.9% (95%CI: 1.4-0.1%) for women. Factors associated with alcohol dependence disorder in men included being single, divorced or widowed compared with married (adjusted OR = 1.47, 95%CI: 1.00-2.14) and being unemployed (adjusted OR=1.30, 95%CI: 1.01-1.67). The highest prevalence of alcohol dependence was among HIV-test unknown TB patients (34.7%), and lowest was among HIV positive patients on treatment but without TB (14.1%), although the difference was not statistically significant (p=0.38). CONCLUSIONS: Male TB/HIV patients in this population have high prevalence of alcohol dependence disorder, and prevalence differs by HIV/TB status. Further work is needed to explore interventions to reduce harmful drinking in this population