The Links Between Migration, Poverty and Health: Evidence From Khayelitsha and Mitchell's Plain

In the mid-1950s, the City of Cape Town was part of a wider area demarcated as a Coloured Labour Preference Area. The free movement of African people into the city was strictly controlled and the residential areas were segregated along racial lines. In terms of Apartheid's grand design, an area designated Mitchell's Plain was demarcated for occupation by Coloured people in 1973 while another designated Khayelitsha was allocated for African people. The two areas were incorporated in one magisterial district, Mitchell's Plain, in the mid-1980s. A sample survey of the area was conducted in late November and early December 2000 with a focus on labour market issues. Its aim was to capture occupants of households aged 18 or older. The survey data has been interrogated to describe the connections between migration, poverty and health in a city where recent rapid urbanisation is changing the demographic profile significantly. As a consequence, the need to provide adequate infrastructure, decent housing and employment poses a daunting challenge ten years after the new democracy has been ushered in.

Participatory evaluation and application of climate smart agriculture practices in mixed smallholder farming systems: a case-study in the semi-arid regions of Kenya

In the first phase of the CCAFS Program (Climate Change, Agriculture and Food security), climate-smart agriculture practices (CSAP) were identified and needed to be tested. In the semi-arid maize-growing areas of Kenya, dry-tolerant varieties and maize-legume intercropping appeared as the most appropriate CSAP, and this paper presents farmer's evaluation of these farming systems. During on-station and on-farm trials in Makueni County, participatory evaluation of intercropping systems of five maize varieties and four beans varieties was conducted. In total, 150 farmers participated; they scored each variety on the basis of several phenotypic criteria and provided an overall score for the variety. Results emphasized the complexity of their varieties' perception. In order to explain the overall score by different agronomic and socio-economic factors, a cumulative mixed model effect was estimated, including random effects for each farmer. Dry-tolerant varieties had a significant lower score, as GLP92 for beans and TEGO for maize. Socio-economic factors including age and gender of the participants influenced the overall score of varieties. We demonstrated that farmers who already purchased improved seed tended to give lower score. Finally, an OLS regression allowed exploring the weight of each phenotypic criterion in the overall score of a maize or bean varieties. This analysis revealed that farmer's perception of a good variety is complex and rely on multiple criteria unlike most of the breeding program mainly based on yield oriented indicators

Trend and Variability in Interannual Air Temperature Over South West Mau Forest, 1985 - 2015

The research is sponsored by Jomo Kenyatta University of Agriculture and Technology and National Commission for Science, Technology and Innovation Kenya. Abstract Globally high altitude forest regions are considered to be more prone to rapid warming. These regions have also shown great seasonal and inter annual temperatures variability. In Kenya mean annual temperatures increased by 1.00C since 1960. Going by global trends it is plausible to argue that high altitude forest areas in Kenya might have shown great seasonal and inter annual temperatures variability over time. This study assessed interannual trend and variability as well as change point detection in average annual air temperature in South West Mau Forest, Kenya between 1985 and 2015. South West Mau Forest is an indigenous montane ecosystem with a tropical montane climate. Annual average air temperature over the South West Mau forest pointed towards climate warming of 0.01880C per year (Kendall’s tau = 0.3677, p value = 0.0033) but with low interannual variability (CV= 0.11%). A shift in the annual average air temperature of 0.3680C at p= 0.0051 was detected between 1985-1998 and 1999- 2015. There was a weak positive anomaly in the annual average air temperature with a slope of 0.0192 and R2 = 0.3074. Overall the region experienced climate warming. Keywords: Climate warming, Trend, Variability, Average temperature DOI: 10.7176/JNSR/11-16-04 Publication date:August 31st 202

Carbon Sequestration by the Above Ground Biomass Pool in the South West Mau Forest of Kenya, 1985 - 2015

Forests are important for regulation of the global carbon balance. Increase in forest biomass enhances atmospheric carbon sequestration while decrease in forest biomass contributes to carbon dioxide emissions. World over, forest biomass has been declining due to forest loss and degradation. The South West Mau has experienced significant forest loss since 1964. The decline is posited to have significant impacts on carbon sequestration, carbon storage, carbon dioxide emissions and status of atmospheric carbon dioxide. This study assessed interannual trend and variability as well as change point detection in carbon sequestration in South West Mau Forest, Kenya between 1985 and 2015. Above ground biomass carbon sequestration was quantified based on the Carnegie-Ames-Stanford Approach (CASA) and carbon fraction for tropical climate domain. Carbon sequestration dynamics were characterized by increase-decrease cycles of approximately 3 years and low interannual variability (CV= 9.13). It emerged that South West Mau Forest was a net carbon emitter with a carbon sequestration balance of -588.40 Kg/ha between 1985 and 2015. Keywords: Forest, Carbon sequestration, Carnegie-Ames-Stanford Approach, Above ground net primary production DOI: 10.7176/JEES/10-8-05 Publication date:August 31st 202

Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Host

Adult schistosomes live in the host's bloodstream where they import nutrients such as glucose across their body surface (the tegument). The parasite tegument is an unusual structure since it is enclosed not by the typical one but by two closely apposed lipid bilayers. Within the tegument two glucose importing proteins have been identified; these are schistosome glucose transporter (SGTP) 1 and 4. SGTP4 is present in the host interactive, apical tegumental membranes, while SGTP1 is found in the tegumental basal membrane (as well as in internal tissues). The SGTPs act by facilitated diffusion. To examine the importance of these proteins for the parasites, RNAi was employed to knock down expression of both SGTP genes in the schistosomula and adult worm life stages. Both qRT-PCR and western blotting analysis confirmed successful gene suppression. It was found that SGTP1 or SGTP4-suppressed parasites exhibit an impaired ability to import glucose compared to control worms. In addition, parasites with both SGTP1 and SGTP4 simultaneously suppressed showed a further reduction in capacity to import glucose compared to parasites with a single suppressed SGTP gene. Despite this debility, all suppressed parasites exhibited no phenotypic distinction compared to controls when cultured in rich medium. Following prolonged incubation in glucose-depleted medium however, significantly fewer SGTP-suppressed parasites survived. Finally, SGTP-suppressed parasites showed decreased viability in vivo following infection of experimental animals. These findings provide direct evidence for the importance of SGTP1 and SGTP4 for schistosomes in importing exogenous glucose and show that these proteins are important for normal parasite development in the mammalian host

The Tegument of the Human Parasitic Worm Schistosoma mansoni as an Excretory Organ: The Surface Aquaporin SmAQP Is a Lactate Transporter

Adult schistosomes are intravascular parasites that metabolize imported glucose largely via glycolysis. How the parasites get rid of the large amounts of lactic acid this generates is unknown at the molecular level. Here, we report that worms whose aquaporin gene (SmAQP) has been suppressed using RNAi fail to rapidly acidify their culture medium and excrete less lactate compared to controls. Functional expression of SmAQP in Xenopus oocytes demonstrates that this protein can transport lactate following Michaelis-Menten kinetics with low apparent affinity (Km = 41±5. 8 mM) and with a low energy of activation (Ea = 7.18±0.7 kcal/mol). Phloretin, a known inhibitor of lactate release from schistosomes, also inhibits lactate movement in SmAQP-expressing oocytes. In keeping with the substrate promiscuity of other aquaporins, SmAQP is shown here to be also capable of transporting water, mannitol, fructose and alanine but not glucose. Using immunofluorescent and immuno-EM, we confirm that SmAQP is localized in the tegument of adult worms. These findings extend the proposed functions of the schistosome tegument beyond its known capacity as an organ of nutrient uptake to include a role in metabolic waste excretion

Comparative performance of the Mbita trap, CDC light trap and the human landing catch in the sampling of Anopheles arabiensis, An. funestus and culicine species in a rice irrigation in western Kenya

BACKGROUND: Mosquitoes sampling is an important component in malaria control. However, most of the methods used have several shortcomings and hence there is a need to develop and calibrate new methods. The Mbita trap for capturing host-seeking mosquitoes was recently developed and successfully tested in Kenya. However, the Mbita trap is less effective at catching outdoor-biting Anopheles funestus and Anopheles arabiensis in Madagascar and, thus, there is need to further evaluate this trap in diverse epidemiological settings. This study reports a field evaluation of the Mbita trap in a rice irrigation scheme in Kenya METHODS: The mosquito sampling efficiency of the Mbita trap was compared to that of the CDC light trap and the human landing catch in western Kenya. Data was analysed by Bayesian regression of linear and non-linear models. RESULTS: The Mbita trap caught about 17%, 60%, and 20% of the number of An. arabiensis, An. funestus, and culicine species caught in the human landing collections respectively. There was consistency in sampling proportionality between the Mbita trap and the human landing catch for both An. arabiensis and the culicine species. For An. funestus, the Mbita trap portrayed some density-dependent sampling efficiency that suggested lowered sampling efficiency of human landing catch at low densities. The CDC light trap caught about 60%, 120%, and 552% of the number of An. arabiensis, An. funestus, and culicine species caught in the human landing collections respectively. There was consistency in the sampling proportionality between the CDC light trap and the human landing catch for both An. arabiensis and An. funestus, whereas for the culicines, there was no simple relationship between the two methods. CONCLUSIONS: The Mbita trap is less sensitive than either the human landing catch or the CDC light trap. However, for a given investment of time and money, it is likely to catch more mosquitoes over a longer (and hence more representative) period. This trap can therefore be recommended for use by community members for passive mosquito surveillance. Nonetheless, there is still a need to develop new sampling methods for some epidemiological settings. The human landing catch should be maintained as the standard reference method for use in calibrating new methods for sampling the human biting population of mosquitoes

Critical amino acid residues in proteins: a BioMart integration of Reactome protein annotations with PRIDE mass spectrometry data and COSMIC somatic mutations

The reversible phosphorylation of serine, threonine and tyrosine hydroxyl groups is an especially prominent form of post-translational modification (PTM) of proteins. It plays critical roles in the regulation of diverse processes, and mutations that directly or indirectly affect these phosphorylation events have been associated with many cancers and other pathologies. Here, we describe the development of a new BioMart tool that gathers data from three different biological resources to provide the user with an integrated view of phosphorylation events associated with a human protein of interest, the complexes of which the protein (modified or not) is a part, the reactions in which the protein and its complexes participate and the somatic mutations that might be expected to perturb those functions. The three resources used are the Reactome, PRIDE and COSMIC databases. The Reactome knowledgebase contains annotations of phosphorylated human proteins linked to the reactions in which they are phosphorylated and dephosphorylated, to the complexes of which they are parts and to the reactions in which the phosphorylated proteins participate as substrates, catalysts and regulators. The PRIDE database holds extensive mass spectrometry data from which protein phosphorylation patterns can be inferred, and the COSMIC database holds records of somatic mutations found in human cancer cells. This tool supports both flexible, user-specified queries and standard (‘canned’) queries to retrieve frequently used combinations of data for user-specified proteins and reactions. We demonstrate using the Wnt signaling pathway and the human c-SRC protein how the tool can be used to place somatic mutation data into a functional perspective by changing critical residues involved in pathway modulation, and where available, check for mass spectrometry evidence in PRIDE supporting identification of the critical residue

Mainstreaming forecast based action into national disaster risk management systems: experience from drought risk management in Kenya

Drought and food security crises heighten risks to lives and livelihoods in East Africa. In recent years, a shift towards acting in advance of such events has gained momentum, notably among the humanitarian and development community. This shift is premised on tools that link climate forecasts with pre-agreed actions and funding, known as Forecast-based Action (FbA), or anticipatory action more widely. While FbA approaches have been developed by a number of humanitarian agencies, the key to scaling-up is mainstreaming these approaches into national risk management systems. This paper addresses this gap in the context of drought risk management in Kenya. We analyse Kenya's current drought management system to assess the potential usability of climate forecast information within the existing system, and outline steps towards improved usability of climate information. Further, we note the critical importance of enabling institutions and reliable financing to ensure that information can be consistently used to trigger early action. We discuss the implications of this for scaling-up FbA into national risk management systems

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London