A comparison of efficiency parameters of SSR markers and genetic diversity analysis in Amorphophallus paeoniifolius (Dennst.) Nicolson

ABSTRACT Amorphophallus (elephant foot yam) is an herbaceous edible aroid crop which belongs to the family Araceae. The study was undertaken to identify the efficient SSR primer that could differentiate a set of 12 elephant foot yam genotypes. Various efficiency parameters, namely, Polymorphism Information Content (PIC), Marker Index (MI), Resolving Power (RP) and Diversity Index (DI) were studied for 11 primers. The relationship between the parameters was studied using Spearman rank correlation coefficient. Discrimination analysis was done to find out the most effective parameter. Finally Principal Coordinate Analysis (PCoA) and dendrogram was done to find out the genetic diversity among the germplasm. The SSR markers under this investigation will facilitate further studies in population genetics and utilization of A. paeoniifolius

Genetic diversity assessment in jute (<em>Corchorus </em>species) utilizing inter simple sequence repeat and simple sequence repeat markers

316-326Fifteen inter simple sequence repeat (ISSR) and five simple sequence repeat (SSR) markers were amplified in ninety eight accessions of both the cultivated species of jute Corchorus olitorius and Corchorus capsularis with the objective to study the genetic diversity of both the species at molecular level. Allelic variability, total number of alleles, and higher polymorphic information content (PIC) suggested ISSR as efficient marker system utilized for the study. Jaccard’s similarity coefficients based on ISSR and combined ISSR-SSR analysis gave useful information for appropriate parent selection for future hybridization programme. Principal coordinate analysis (PCoA) supported UPGMA based cluster analysis and species speciation occurred at molecular level