Morphologic and morphometric study of the musculus obliquus dorsalis of the dog

In the present investigation, the dorsal oblique muscle of the right ocular globe was removed from six adult dogs weighing 40-50 kg and analyzed by light microscopy. Muscle samples were taken from the central portion of the muscle belly, subsequently ultrafrozen, cut and stained with m-ATPase at pH 4.6. Fibers were classified as type I or type II according to their reaction to the m-ATPase and detailed morphologic and morphometric studies were made. The muscles showed two clearly distinct layers, a central layer and a peripheral layer, mainly composed of type II fibers. The fibers in the central layer were larger in size than those in the peripheral layer. The peculiar stratigraphy of the dorsal oblique muscle should be taken into account when performing analyses of this muscle and investigating the significance of the fiber types it contains.En el presente trabajo se han extraído, de los globos oculares derechos, de seis perros adultos de 40-50 kilogramos de peso, los músculos oblicuos dorsales y analizados con microscopia de luz. Las muestras musculares para su análisis se tomaron de la porción central del vientre muscular y fueron ultracongeladas, cortadas y teñidas por la técnica de m-ATPasa a pH 4,6. Las fibras se clasificaron en tipo I y tipo II en virtud a su reacción frente a la m-ATPasa, realizándose un detallado estudio morfológico y morfométrico. Los músculos presentaron dos estratos netamente diferenciados, uno central y otro periférico, compuestos mayoritariamente por fibras tipo II. Las fibras del estrato central presentaron un mayor tamaño que las correspondientes del estrato periférico. La peculiar estratigrafía del músculo oblicuo dorsal debería tenerse en consideración al analizar este músculo e investigar el significado de los tipos fibrilares que contien

Variations in the basic biokinematic pattern in three breeds of horses at the hand-led walk

In this study the lineal and temporal parameters of the stride of andalusian (n=10), angloarabian (n=6) and arabian (n=7) mature horses were compared at the walk. The speeds were similar in the three breeds (1.63±0.10 m/s), for what the Archivos de zootecnia vol. 48, núm. 183, p. 328. GALISTEO ET AL. statistical comparisons were carried out by an analysis of the variance, and a means comparison test (Tukey test). The stride lenght was shorter in arabians and andalusians than in angloarabians, while the overtracking lenght was bigger in the arabians. The duration of the forelimb cycle was bigger in angloarabians due to a bigger duration in this breed of the stance phase. The midstace position (expressed as a percentaje of the stride duration) differed among the three breeds, indicating that each one shows times of braking and propulsion of the forelimb different to the other ones; but not in the instant in which the hoof reaches the highest point in its trajectory. In the hind limb the differences were much less marked, since small differences were detected in the duration of the cycle between arabians and angloarabians, while the stride duration was longer in andalusians and angloarabians than in the Arabians; the braking and propulsion phases (determined by the position along the stride of the midstance position) didn’t differ among breeds, the same with the instant in which the hindhoof reach the highest point in its trajectory. It can be concluded that marked differences exist between equine breeds in the lineal parameters of the stride at the walk, and the temporal parameters of the forelimb, while in the hind limb are less marked, probably because this member is functionally committed in the propulsion of the horse.En este estudio se compararon los parámetros lineales y temporales del tranco de paso de caballos adultos de Pura Raza Española (n=10), Angloárabe (n=6) y Árabe (n=7). Las velocidades fueron similares en las tres razas (1,63±0,10 m/s), por lo que las comparaciones estadísticas se realizaron mediante un análisis de la varianza y un test de comparación de medias (Test de Tukey). La amplitud de tranco fue más corta en Árabes y Españoles que en Angloárabes, mientras que la longitud de sobrehuella fue mayor en los Árabes. La duración del ciclo del miembro torácico fue mayor en Angloárabes debido a una mayor duración en esta raza de la fase de apoyo. La posición de apoyo medio (expresada porcentualmente a la duración total del ciclo) difirió entre las tres razas, indicando que cada raza presenta tiempos de frenada y propulsión del miembro torácico diferentes a las demás; no así en el instante en el que el casco alcanza el punto más alto de su trayectoria. En el miembro pelviano las diferencias fueron mucho menos marcadas, ya que se detectaron pequeñas diferencias en la duración del ciclo entre Árabes y Angloárabes, mientras que la duración del tranco fue mayor en Españoles y Angloárabes que en los Árabes; los tiempos de frenada y propulsión (determinada por la posición a lo largo del tranco del apoyo medio) no difirió entre razas, lo mismo que el instante en que el casco pelviano se encuentra en el punto más alto de su trayectoria. Se puede concluir que existen marcadas diferencias entre razas equinas en los parámetros lineales del tranco al paso, al igual que sucede con los parámetros temporales del miembro torácico, mientras que en el pelviano son menos marcadas, probablemente porque este miembro está funcionalmente más comprometido que el torácico en la propulsión del caballo

Modeling Optical and Electronic Properties of Silica Nano-Clusters in Silicon Rich Oxide Films

Quantum effects are very important in nano scale systems such as molecules and clusters constituted of particles from a few to hundreds or a few thousands of atoms. Their optical and electronic properties are often dependent on the size of the systems and the way in which the atoms in these molecules or clusters are bonded. Generally, these nano-structures display optical and electronic properties significantly different of the bulk materials. Silica agglomerates expected in Silicon Rich Oxide (SRO) films have optical properties, which depend directly on size, and their rationalization can lead to new applications with a potential impact on many fields of science and technology. On the other hand, the room temperature photoluminescence (PL) of Si : SiO2 or Si : SiOx structures usually found in SRO has recently generated an enormous interest due to their possible applications in optoelectronic devices. However, the understanding of the emission mechanism is still under debate. In this research, we employed the Density Functional Theory with a functional B3LYP and a basis set 6-31 G* to calculate the electronic and optical properties of molecules and clusters of silicon dioxide. With the theoretical calculation of the structural and optical properties of silicon dioxide clusters is possible to evaluate the contribution of silica in the luminescent emission mechanism experimentally found in thin SRO films. It was found that silica contribution to the luminescent phenomenon in SRO thin films is less important than that of the silicon monoxide agglomerates because the number of silica structures, which may show emission in the visible spectrum, is much lower [1], compared to the number of silicon monoxide structures which emit in this region. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3192

A policy-based containerized filter for secure information sharing in organizational environments

In organizational environments, sensitive information is unintentionally exposed and sent to the cloud without encryption by insiders that even were previously informed about cloud risks. To mitigate the effects of this information privacy paradox, we propose the design, development and implementation of SecFilter, a security filter that enables organizations to implement security policies for information sharing. SecFilter automatically performs the following tasks: (a) intercepts files before sending them to the cloud; (b) searches for sensitive criteria in the context and content of the intercepted files by using mining techniques; (c) calculates the risk level for each identified criterion; (d) assigns a security level to each file based on the detected risk in its content and context; and (e) encrypts each file by using a multi-level security engine, based on digital envelopes from symmetric encryption, attribute-based encryption and digital signatures to guarantee the security services of confidentiality, integrity and authentication on each file at the same time that access control mechanisms are enforced before sending the secured file versions to cloud storage. A prototype of SecFilter was implemented for a real-world file sharing application that has been deployed on a private cloud. Fine-tuning of SecFilter components is described and a case study has been conducted based on document sharing of a well-known repository (MedLine corpus). The experimental evaluation revealed the feasibility and efficiency of applying a security filter to share information in organizational environmentsThis work has been partially supported by the Spanish “Ministerio de Economia y Competitividad” under the project grant TIN2016-79637-P “Towards Unification of HPC and Big Dataparadigms”

Morphometric study of the nerve fibers of the oculomotor nerve in dog

The intracranial portion of the right oculomotor nerves was extracted from six adult German Shepherd dogs. The nerves were studied using light and electron microscopy. Nerve area was calculated, and myelinated and unmyelinated fibers were analized to determine number, diameter and area. Also in the myelinated fibers the area and diameter of the correspondent axon, and thickness of the myeline sheath were measured. The mean number of fibers was 8543.50±1231.85 for myelinated and 1402.00±241.58 for unmyelinated. The mean fiber diameter was 10.23±0.68 μm for myelinated and 0.43±0.21 μm for unmyelinated. This study demonstrated that the oculomotor nerves in dogs have structural and ultrastructural characteristics that are similar to those from other specie

Valor pronóstico de la desnutrición en pacientes con insuficiencia cardíaca aguda y su influencia en la interpretación de marcadores de congestión venosa sistémica

Antecedentes La desnutrición es frecuente en los pacientes con insuficiencia cardíaca (IC). Dicha situación contribuye al incremento de la congestión sistémica dificultando el manejo clínico. Cuantificar la desnutrición y su relación con la congestión sistémica, es importante para optimizar el tratamiento durante la fase aguda. Material y métodos Estudio de cohortes retrospectivo en pacientes con diagnóstico de IC aguda. La población se estratificó según los índices de nutrición de CONUT (Controlling Nutritional Status) y PNI (Prognostic Nutritional Index) con el objetivo de analizar su relación con parámetros objetivos de congestión y el valor pronóstico al año de seguimiento. Resultados Se incluyeron un total de 309 pacientes, presentando más de la mitad algún grado de desnutrición al ingreso. El grado de congestión fue significativamente superior en los pacientes desnutridos, con una mayor proporción de «líneas b» y un mayor volumen plasmático relativo. Las concentraciones de la prohormona N-terminal del péptido natriurético cerebral (NT-proBNP), tanto al ingreso como al alta, también fueron significativamente superiores en los pacientes desnutridos, independientemente de la escala empleada. El análisis univariante identificó el índice de CONUT y PNI, como factores asociados a la mortalidad al año para todas las causas (HR 1, 62 [1, 22-2, 14]; p = 0, 001) y de PNI (HR 0, 65 [0, 53-0, 80]; p = < 0, 001), respectivamente. Conclusiones Un mayor grado de desnutrición (determinado mediante los índices de CONUT y PNI) en pacientes con IC aguda, se asoció a una mayor presencia de parámetros objetivos de congestión y a una mayor mortalidad al año para todas las causas. Background Malnutrition is frequent in patients with heart failure (HF) and contributes to increased systemic congestion, but also hinders its correct assessment, especially during decompensations. Estimating the degree of malnutrition and its relationship with systemic congestion is important to optimize treatment during decompensations. Material and methods Retrospective cohort study in patients with acute HF. The population was stratified according to CONUT (Controlling Nutritional Status) and PNI (Prognostic Nutritional Index) nutrition indices in order to analyse their relationship with objective parameters of congestion and the prognostic value of malnutrition. Results 309 patients were included. More than half presented some degree of malnutrition upon admission. The degree of congestion was significantly higher in malnourished patients, with a higher proportion of «comet tail artifacts» and a higher relative plasma volume. NT-proBNP concentrations, both on admission and at discharge, were also significantly higher in malnourished patients, regardless of the scale used. The univariate analysis identified the CONUT and PNI index as factors associated with one-year mortality from any cause (HR 1.62 [1.22-2.14]; p = 0.001) and PNI (HR 65 [0.53-0.80]; p = < 0.001), respectively. Conclusions A higher degree of malnutrition (determined by means of the CONUT and PNI indices) in patients with acute HF was associated with a higher presence of objective parameters of congestion and a higher one-year all-cause mortality

Whole genome sequencing of Shigella sonnei through PulseNet Latin America and Caribbean: advancing global surveillance of foodborne illnesses

Objectives Shigella sonnei is a globally important diarrhoeal pathogen tracked through the surveillance network PulseNet Latin America and Caribbean (PNLA&C), which participates in PulseNet International. PNLA&C laboratories use common molecular techniques to track pathogens causing foodborne illness. We aimed to demonstrate the possibility and advantages of transitioning to whole genome sequencing (WGS) for surveillance within existing networks across a continent where S. sonnei is endemic. Methods We applied WGS to representative archive isolates of S. sonnei (n = 323) from laboratories in nine PNLA&C countries to generate a regional phylogenomic reference for S. sonnei and put this in the global context. We used this reference to contextualise 16 S. sonnei from three Argentinian outbreaks, using locally generated sequence data. Assembled genome sequences were used to predict antimicrobial resistance (AMR) phenotypes and identify AMR determinants. Results S. sonnei isolates clustered in five Latin American sublineages in the global phylogeny, with many (46%, 149 of 323) belonging to previously undescribed sublineages. Predicted multidrug resistance was common (77%, 249 of 323), and clinically relevant differences in AMR were found among sublineages. The regional overview showed that Argentinian outbreak isolates belonged to distinct sublineages and had different epidemiologic origins. Conclusions Latin America contains novel genetic diversity of S. sonnei that is relevant on a global scale and commonly exhibits multidrug resistance. Retrospective passive surveillance with WGS has utility for informing treatment, identifying regionally epidemic sublineages and providing a framework for interpretation of prospective, locally sequenced outbreaks

The Spanish Infrared Camera onboard the EUSO-BALLOON (CNES) flight on August 24, 2014

The EUSO-Balloon (CNES) campaign was held during Summer 2014 with a launch on August 24. In the gondola, next to the Photo Detector Module (PDM), a completely isolated Infrared camera was allocated. Also, a helicopter which shooted flashers flew below the balloon. We have retrieved the Cloud Top Height (CTH) with the IR camera, and also the optical depth of the nonclear atmosphere have been inferred with two approaches: The first one is with the comparison of the brightness temperature of the cloud and the real temperature obtained after the pertinent corrections. The second one is by measuring the detected signal from the helicopter flashers by the IR Camera, considering the energy of the flashers and the location of the helicopter

The exposure of the hybrid detector of the Pierre Auger Observatory

The Pierre Auger Observatory is a detector for ultra-high energy cosmic rays. It consists of a surface array to measure secondary particles at ground level and a fluorescence detector to measure the development of air showers in the atmosphere above the array. The "hybrid" detection mode combines the information from the two subsystems. We describe the determination of the hybrid exposure for events observed by the fluorescence telescopes in coincidence with at least one water-Cherenkov detector of the surface array. A detailed knowledge of the time dependence of the detection operations is crucial for an accurate evaluation of the exposure. We discuss the relevance of monitoring data collected during operations, such as the status of the fluorescence detector, background light and atmospheric conditions, that are used in both simulation and reconstruction.Comment: Paper accepted by Astroparticle Physic