Strategies Used by Employment Service Providers in the Job Development Process: Are they consistent with what employers want?

Historically, the role of job developers employed in the state/federal vocational rehabilitation program and the larger network of community-based rehabilitation programs has been to identify and secure paid employment for individuals with disabilities, particularly those with significant disabilities. This technical report describes the results of a study of job development/job placement professionals' strategies in the employment process, and compares these results to employer perceptions of the employment process from recent literature. The report also identifies implications for job development/placement practice based on this comparative analysis

FDTD calculations of the diffraction coefficient of vibrating wedges

pre-printThe full-vector Maxwell's equations finite-difference time-domain (FDTD) method is employed to calculate diffraction coefficients of vibrating conducting and dielectric wedges. Two-dimensional FDTD models of right-angle wedges are constructed to include total-field scattered-field incident plane-wave source conditions as well as convolutional perfectly matched-layer boundary conditions. These models are first validated by calculating the diffraction coefficient of a stationary perfect electrical conducting (PEC) right-angle wedge for comparison to uniform geometrical theory of diffraction (UTD) analytical solutions. Next, a brute-force FDTD technique for modeling wedge vibrations is utilized to calculate the Doppler diffraction coefficients of vibrating lossless and lossy wedges

Isospin-breaking interactions studied through mirror energy differences

Background: Information on charge-dependent (i.e., isospin-non-conserving) interactions is extracted from excited states of mirror nuclei. Purpose: Specifically, the purpose of the study is to extract effective isovector (Vpp 12Vnn) interactions which, in general, can either be of Coulomb or nuclear origin. Methods: A comprehensive shell-model description of isospin-breaking effects is used to fit data on mirror energy differences in the A = 42\u201354 region. The angular-momentum dependence of isospin-breaking interactions was determined from a systematic study of mirror energy differences. Results: The results reveal a significant isovector term, with a very strong spin dependence, beyond that expected of a two-body Coulomb interaction. Conclusions: The isospin-breaking terms that are extracted have a J dependence that is not consistent with the known CSB properties of the bare nucleon-nucleon interaction

Epidemiological and molecular investigations into environmental Salmonella in NSW, Australia: a One Health approach

Salmonella Wangata is a common yet poorly described serotype in New South Wales (NSW), Australia, that is likely being transmitted from an environmental reservoir. To date, routine follow up of notified cases by public health authorities have failed to identify a common source. This thesis aimed to investigate S. Wangata using a One Health approach. As a first step, data from humans, food, domestic animals and wildlife were collated and compared to establish an overview of the diversity of Salmonella serotypes in NSW. An investigation into notified human cases of S. Wangata was then conducted from November 2016 to April 2017 in NSW. Identified cases were interviewed and samples were collected from cases’ home environment. Samples were also collected from wildlife in rehabilitative care. Whole genome sequencing was used to determine the phylogeny and characteristics of S. Wangata. To explore if S. Wangata was associated with landscape risk factors, a conditional autoregressive analysis was performed on human surveillance data from between 1 January 2001 and 31 December 2015. It was found that Salmonella data from non-human sources were lacking. Putative risk factors for infection with S. Wangata were identified and isolates of S. Wangata were recovered from household and wildlife samples. Genomic analysis revealed that isolates of S. Wangata were clonal, supporting the hypothesis that S. Wangata is transmitted via an environmental source. The strong association between S. Wangata and environmental reservoirs was again supported by the spatial analysis, however it was unclear if there was a specific wildlife reservoir. This thesis presents the most detailed understanding of S. Wangata epidemiology to date. All the investigations in this thesis were underpinned by the integration of human, animal and environmental health and demonstrate the benefits of using a One Health approach in the investigation of environmental Salmonella

The development and characterisation of a bacterial artificial chromosome library for Fragaria vesca

<p>Abstract</p> <p>Background</p> <p>The cultivated strawberry <it>Fragaria ×ananassa </it>is one of the most economically-important soft-fruit species. Few structural genomic resources have been reported for <it>Fragaria </it>and there exists an urgent need for the development of physical mapping resources for the genus. The first stage in the development of a physical map for <it>Fragaria </it>is the construction and characterisation of a high molecular weight bacterial artificial chromosome (BAC) library.</p> <p>Methods</p> <p>A BAC library, consisting of 18,432 clones was constructed from <it>Fragaria vesca </it>f. <it>semperflorens </it>accession 'Ali Baba'. BAC DNA from individual library clones was pooled to create a PCR-based screening assay for the library, whereby individual clones could be identified with just 34 PCR reactions. These pools were used to screen the BAC library and anchor individual clones to the diploid <it>Fragaria </it>reference map (FV×FN).</p> <p>Findings</p> <p>Clones from the BAC library developed contained an average insert size of 85 kb, representing over seven genome equivalents. The pools and superpools developed were used to identify a set of BAC clones containing 70 molecular markers previously mapped to the diploid <it>Fragaria </it>FV×FN reference map. The number of positive colonies identified for each marker suggests the library represents between 4× and 10× coverage of the diploid <it>Fragaria </it>genome, which is in accordance with the estimate of library coverage based on average insert size.</p> <p>Conclusion</p> <p>This BAC library will be used for the construction of a physical map for <it>F. vesca </it>and the superpools will permit physical anchoring of molecular markers using PCR.</p

Immunophenotyping and Efficacy of Low Dose ATG in Non-Sensitized Kidney Recipients Undergoing Early Steroid Withdrawal: A Randomized Pilot Study

Rabbit antithymocyte globulin (ATG) is commonly used as an induction therapy in renal transplant recipients, but the ideal dosage in tacrolimus-based early steroid withdrawal protocols has not been established. The purpose of this pilot study was to determine the immunophenotyping and efficacy of lower dose ATG in low immunological-risk kidney transplant recipients. In this prospective study, 45 patients were randomized (1∶1) to our standard dose ATG (total dose 3.75 mg/kg)(sATG) vs. lower dose 2.25 mg/kg (lowATG). All patients underwent early steroid withdrawal within 7 days. The primary end point was biopsy-proven acute rejection at 12 months. Prospective immunophenotyping of freshly isolated PBMCs was performed at baseline, 3, 6, 12 months post-transplant. The rate of acute rejection was 17% and 10% in the sATG and lowATG, respectively. Effector memory T cells, Tregs and recent thymic emigrants T cells had similar kinetics post-transplant in both groups. No statistically significant differences were found in graft survival, patient survival or infections between the two groups, though there was a non-significant increase in leukopenia (43%v s. 30%), CMV (8% vs. 0) and BK (4% vs. 0) infections in sATG group vs. lowATG. In sum, in low immunological risk kidney recipients undergoing steroid withdrawal, low dose ATG seems to be efficacious in preventing acute rejection and depleting T cells with potentially lower infectious complications. A larger study is warranted to confirm these findings. Trial Registration ClinicalTrials.gov NCT0054840

Triplet energy differences and the low lying structure of Ga 62

Background: Triplet energy differences (TED) can be studied to yield information on isospin-non-conserving interactions in nuclei. Purpose: The systematic behavior of triplet energy differences (TED) of T=1, J\u3c0=2+ states is examined. The A=62 isobar is identified as having a TED value that deviates significantly from an otherwise very consistent trend. This deviation can be attributed to the tentative assignments of the pertinent states in Ga62 and Ge62. Methods: An in-beam \u3b3-ray spectroscopy experiment was performed to identify excited states in Ga62 using Gamma-Ray Energy Tracking In-Beam Nuclear Array with the S800 spectrometer at NSCL using a two-nucleon knockout approach. Cross-section calculations for the knockout process and shell-model calculations have been performed to interpret the population and decay properties observed. Results: Using the systematics as a guide, a candidate for the transition from the T=1, 2+ state is identified. However, previous work has identified similar states with different J\u3c0 assignments. Cross-section calculations indicate that the relevant T=1, 2+ state should be one of the states directly populated in this reaction. Conclusions: As spins and parities were not measurable, it is concluded that an unambiguous identification of the first T=1, 2+ state is required to reconcile our understanding of TED systematics

Protein status elicits compensatory changes in food intake and food preferences123

Background: Protein is an indispensable component within the human diet. It is unclear, however, whether behavioral strategies exist to avoid shortages

Healthy eating and lifestyle in pregnancy (HELP): a cluster randomised trial to evaluate the effectiveness of a weight management intervention for pregnant women with obesity on weight at 12 months postpartum

Objective: To assess whether a weight management intervention for pregnant women with obesity was effective in reducing body mass index (BMI) 12 months after giving birth. Methods: Pragmatic, cluster randomised controlled trial (RCT) with embedded cost-effectiveness analysis. 598 women with a BMI of ≥30 kg/m2 (between 12 and 20 weeks gestation) were recruited from 20 secondary care maternity units in England and Wales. BMI at 12 months postpartum was the primary outcome. A range of clinical and behavioural secondary outcomes were examined. Interventions: Women attending maternity units randomised to intervention were invited to a weekly weight management group, which combined expertise from a commercial weight loss programme with clinical advice from midwives. Both intervention and control participants received usual care and leaflets on diet and physical activity in pregnancy. Results: Mean (SD) BMI at 12 months postpartum was 36.0 kg/m2 (5.2) in the control group, and 37.5 kg/m2 (6.7) in the intervention group. After adjustment for baseline BMI, the intervention effect was −0.02 (95% CI −0.04 to 0.01). The intervention group had an improved healthy eating score (3.08, 95% CI 0.16 to 6.00, p < 0.04), improved fibre score (3.22, 1.07 to 5.37, p < 0.01) and lower levels of risky drinking at 12 months postpartum compared to the control group (OR 0.45, 0.27 to 0.74, p < 0.002). The net incremental monetary benefit was not statistically significantly different between arms, although the probability of the intervention being cost-effective was above 60%, at policy-relevant thresholds. Conclusions: There was no significant difference between groups on the primary outcome of BMI at 12 months. Analyses of secondary outcomes indicated improved healthy eating and lower levels of risky drinking. Trial registration: Current Controlled Trials ISRCTN25260464

Soil fungal community shift evaluation as a potential cadaver decomposition indicator

Fungi metabolise organic matter in situ and so alter both the bio-/physico-chemical properties and microbial community structure of the ecosystem. In particular, they are responsible reportedly for specific stages of decomposition. Therefore, this study aimed to extend previous bacteria-based forensic ecogenomics research by investigating soil fungal community and cadaver decomposition interactions in microcosms with garden soil (20 kg, fresh weight) and domestic pig (Sus scrofa domesticus) carcass (5 kg, leg). Soil samples were collected at depths of 0–10 cm, 10–20 cm and 20–30 cm on days 3, 28 and 77 in the absence (control −Pg) and presence (experimental +Pg) of Sus scrofa domesticus and used for total DNA extraction and nested polymerase chain reaction and denaturing gradient gel electrophoresis (PCR–DGGE) profiling of the 18S rRNA gene. The Shannon–Wiener (H′) community diversity indices were 1.25 ± 0.21 and 1.49 ± 0.30 for the control and experimental microcosms, respectively, while comparable Simpson species dominance (S) values were 0.65 ± 0.109 and 0.75 ± 0.015. Generally, and in contrast to parallel studies of the bacterial 16S rRNA and 16S rDNA profiles, statistical analysis (t-test) of the 18S dynamics showed no mathematically significant shifts in fungal community diversity (H′; p = 0.142) and dominance (S; p = 0.392) during carcass decomposition, necessitating further investigations