A lightning climatology of northeast India:1998-2007

About 55,000 lightning flashes were recorded by the Lightning Imaging Sensor (LIS) during the 10-year period (1998-2007) in northeast India (NEI) and were analyzed to study their total climatology, seasonal, annual and inter-annual variations. The results suggest that the western region of the northeast India is prone to a maximum amount of lightning activity and the northeast and eastern part is minimal in activity. The activity shifts west and east as the season advances. It is stagnant in the western and southwestern region in the last season of the year. The highest percentage of activity is observed during the pre-monsoon season. During the annual course the activity exhibits bimodal variation giving an average value of 8.4 flashes/pass. The time series analysis of the parameter shows that the activity over NEI shows a consistent increase from 1998 to 2002 and then onwards it shows a consistent decrease. The activity in December is a characteristic feature for the region. The highest magnitude of activity is noticed in the year 2001 with 20 flashes/pass

Cloud liquid water content responses to hygroscopic seeding of warm clouds

The cloud liquid water content (CLWC) data in time and space from a total of 96 pairs of target (T) and control (C) experiments were analysed in this study to compare the responses of CLWC to hygroscopic seeding of warm clouds. Our results of various approaches taken for this analysis have indicated significant modifications in the CLWC for the T clouds as against C clouds. Analysis of changes in CLWC in the T clouds after and before seeding has pointed out their increasing trend of values with increment in the number of seeded traverses in most cases. These results have shown that CLWC in the T clouds increases following the seeding treatment in the range 9-26. Similar comparisons in the C clouds have indicated obvious diminution in CLWC that lies in the range 5-11. These results clearly indicate the influence on microphysical growth and decay of such clouds that arises from hygroscopic seeding and not seeding of warm clouds. Analysis of spatial responses of CLWC to seeding has shown that the optimum effect of seeding may be achieved for a suitable cloud in the altitude range 5750-6250 ft (asl) in the Pune area. It is believed that this study has provided adequate support in favour of the hypothesis of hygroscopic seeding of warm clouds

Markov chain models for pre-monsoon season thunderstorms over Pune

The probabilistic distribution of the thunderstorm phenomenon during the pre-monsoon season (1 March to 18 June) over Pune, a tropical Indian station, has been examined with the help of Markov chain models using daily thunderstorm data for a period of 11 years (1970-80). The data have also been tested using Akaike's information criterion. This test has clearly indicated that the first-order Markov chain model is the best fit model for thunderstorm forecasting, which has described the appropriate period (8 days) of occurrence of thunderstorm phenomenon over Pune. Further, the steady-state probabilities and mean recurrence time of thunderstorm days and non-thunderstorm days have also been calculated for the first- and second-order Markov chain models. These computations have revealed that the observed and theoretical values of steady-state probabilities are realistically matched

Diurnal variation of lightning activity over the Indian region

Satellite (LIS) based lightning flash grid (0.5° × 0.5°) data for the Indian land mass region covering from 8°–33°N and 73°–86°E for a period of 4 years (1998–2001) were used to study the diurnal variation with one hour time resolution. The analysis revealed that there exists a strong diurnal cycle in the lightning activity with a prominent peak around 1000 UTC. An examination of seasonal diurnal variation suggests that the lightning activity was found highest in premonsoon and lowest in the postmonsoon seaso

Study of thunderstorm and rainfall activity over the Indian region

Thirty years (1951-1980) of mean monthly thunderstorms days (TS) and rainfall (RF) amounts for 260 Indian observatories spread uniformly over the country were used to obtain their monthly, seasonal and zonal percentage of occurrence from all India totals. The study has revealed that there is a time lag of one month in the occurrence of peak activity of TS and RF. Seasonal analysis of these two parameters suggest that rainfall yield associated with postmonsoon season TS seems to be higher than the premonsoon season. Six zone analysis of TS and RF has suggested that there exists a wide range of variation in both parameters month after month in that zones, but the 30 year mean percentage of occurrence seems to be more or less equal in magnitude in each zone

WEE1 inhibition sensitizes osteosarcoma to radiotherapy

<p>Abstract</p> <p>Background</p> <p>The use of radiotherapy in osteosarcoma (OS) is controversial due to its radioresistance. OS patients currently treated with radiotherapy generally are inoperable, have painful skeletal metastases, refuse surgery or have undergone an intralesional resection of the primary tumor. After irradiation-induced DNA damage, OS cells sustain a prolonged G₂cell cycle checkpoint arrest allowing DNA repair and evasion of cell death. Inhibition of WEE1 kinase leads to abrogation of the G₂arrest and could sensitize OS cells to irradiation induced cell death.</p> <p>Methods</p> <p>WEE1 expression in OS was investigated by gene-expression data analysis and immunohistochemistry of tumor samples. WEE1 expression in OS cell lines and human osteoblasts was investigated by Western blot. The effect of WEE1 inhibition on the radiosensitivity of OS cells was assessed by cell viability and caspase activation analyses after combination treatment. The presence of DNA damage was visualized using immunofluorescence microscopy. Cell cycle effects were investigated by flow cytometry and WEE1 kinase regulation was analyzed by Western blot.</p> <p>Results</p> <p>WEE1 expression is found in the majority of tested OS tissue samples. Small molecule drug PD0166285 inhibits WEE1 kinase activity. In the presence of WEE1-inhibitor, irradiated cells fail to repair their damaged DNA, and show higher levels of caspase activation. The inhibition of WEE1 effectively abrogates the irradiation-induced G₂arrest in OS cells, forcing the cells into premature, catastrophic mitosis, thus enhancing cell death after irradiation treatment.</p> <p>Conclusion</p> <p>We show that PD0166285, a small molecule WEE1 kinase inhibitor, can abrogate the G₂checkpoint in OS cells, pushing them into mitotic catastrophe and thus sensitizing OS cells to irradiation-induced cell death. This suggests that WEE1 inhibition may be a promising strategy to enhance the radiotherapy effect in patients with OS.</p

Down Regulation of a Matrix Degrading Cysteine Protease Cathepsin L, by Acetaldehyde: Role of C/EBPα

BACKGROUND: The imbalance between extra cellular matrix (ECM) synthesis and degradation is critical aspect of various hepatic pathologies including alcohol induced liver fibrosis. This study was carried out to investigate the effect of acetaldehyde on expression of an extra cellular matrix degrading protease cathepsin L (CTSL) in HepG2 cells. METHODOLOGY AND RESULTS: We measured the enzymatic activity, protein and, mRNA levels of CTSL in acetaldehyde treated and untreated cells. The binding of CAAT enhancer binding protein α (C/EBP α) to CTSL promoter and its key role in the transcription from this promoter and conferring responsiveness to acetaldehyde was established by site directed mutagenesis, electrophoretic mobility shift assay (EMSA), chromatin immunoprecipitation (ChIP) assays and siRNA technology. Acetaldehyde treatment significantly decreased CTSL activity and protein levels in HepG2 cells. A similar decrease in the mRNA levels and promoter activity was also observed. This decrease by acetaldehyde was attributed to the fall in the liver enriched transcription factor C/EBP α levels and it's binding to the CTSL promoter. Mutagenesis of C/EBP α binding motifs revealed the key role of this factor in CTSL transcription as well as conferring responsiveness to acetaldehyde. The siRNA mediated silencing of the C/EBP α expression mimicked the effect of acetaldehyde on CTSL levels and its promoter activity. It also abolished the responsiveness of this promoter to acetaldehyde. CONCLUSION: Acetaldehyde down regulates the C/EBP α mediated CTSL expression in hepatic cell lines. The decreased expression of CTSL may at least in part contribute to ECM deposition in liver which is a hallmark of alcoholic liver fibrosis

Protection of pancreatic INS-1 β-cells from glucose- and fructose-induced cell death by inhibiting mitochondrial permeability transition with cyclosporin A or metformin

Hyperglycemia is detrimental to β-cell viability, playing a major role in the progression of β-cell loss in diabetes mellitus. The permeability transition pore (PTP) is a mitochondrial channel involved in cell death. Recent evidence suggests that PTP inhibitors prevent hyperglycemia-induced cell death in human endothelial cells. In this work, we have examined the involvement of PTP opening in INS-1 cell death induced by high levels of glucose or fructose. PTP regulation was studied by measuring the calcium retention capacity in permeabilized INS-1 cells and by confocal microscopy in intact INS-1 cells. Cell death was analyzed by flow cytometry. We first reported that metformin and cyclosporin A (CsA) prevented Ca2+-induced PTP opening in permeabilized and intact INS-1 cells. We then showed that incubation of INS-1 cells in the presence of 30 mM glucose or 2.5 mM fructose induced PTP opening and led to cell death. As both metformin and CsA prevented glucose- and fructose- induced PTP opening, and hampered glucose- and fructose- induced cell death, we conclude that PTP opening is involved in high glucose- and high fructose- induced INS-1 cell death. We therefore suggest that preventing PTP opening might be a new approach to preserve β-cell viability

Search for rare quark-annihilation decays, B --> Ds(*) Phi

We report on searches for B- --> Ds- Phi and B- --> Ds*- Phi. In the context of the Standard Model, these decays are expected to be highly suppressed since they proceed through annihilation of the b and u-bar quarks in the B- meson. Our results are based on 234 million Upsilon(4S) --> B Bbar decays collected with the BABAR detector at SLAC. We find no evidence for these decays, and we set Bayesian 90% confidence level upper limits on the branching fractions BF(B- --> Ds- Phi) Ds*- Phi)<1.2x10^(-5). These results are consistent with Standard Model expectations.Comment: 8 pages, 3 postscript figues, submitted to Phys. Rev. D (Rapid Communications

Measurement of the polarisation of W bosons produced with large transverse momentum in pp collisions at sqrt(s) = 7 TeV with the ATLAS experiment

This paper describes an analysis of the angular distribution of W->enu and W->munu decays, using data from pp collisions at sqrt(s) = 7 TeV recorded with the ATLAS detector at the LHC in 2010, corresponding to an integrated luminosity of about 35 pb^-1. Using the decay lepton transverse momentum and the missing transverse energy, the W decay angular distribution projected onto the transverse plane is obtained and analysed in terms of helicity fractions f0, fL and fR over two ranges of W transverse momentum (ptw): 35 < ptw < 50 GeV and ptw > 50 GeV. Good agreement is found with theoretical predictions. For ptw > 50 GeV, the values of f0 and fL-fR, averaged over charge and lepton flavour, are measured to be : f0 = 0.127 +/- 0.030 +/- 0.108 and fL-fR = 0.252 +/- 0.017 +/- 0.030, where the first uncertainties are statistical, and the second include all systematic effects.Comment: 19 pages plus author list (34 pages total), 9 figures, 11 tables, revised author list, matches European Journal of Physics C versio