Norepinephrine-induced loss of phosphatidylinositol from isolated rat liver plasma membrane Effects of divalent cations

AbstractNorepinephrine at 5 μM induces a rapid (60 s) and specific loss of phosphatidylinositol (PtdIns) when added to isolated rat liver plasma membranes. The hormone action is inhibited by the α-adrenergic antagonist phentolamine (20 μM). Depletion of Mg2+ and Ca2+ singly or in combination from the incubation buffer mimicks the hormone effect on PtdIns breakdown. No further effect on PtdIns degradation could be measured when norepinephrine was added to the cation-depleted buffers. Addition of the Ca2+ ionophore A23187 to the isolated membranes has no effect. It is concluded that PtdIns degradation can be provoked in isolated rat liver plasma membrane through α-adrenergic receptor activation and that this effect is dependent on divalent cations in the sense that loss of cations from the membrane allows degradation to commence

The Spectroscopic Orbit of the Planetary Companion Transiting HD209458

We report a spectroscopic orbit with period P = 3.52433 +/- 0.00027 days for the planetary companion that transits the solar-type star HD209458. For the metallicity, mass, and radius of the star we derive [Fe/H] = 0.00 +/- 0.02, M = 1.1 +/- 0.1 solar masses, and R = 1.3 +/- 0.1 solar radii. This is based on a new analysis of the iron lines in our HIRES template spectrum, and also on the absolute magnitude and color of the star, and uses isochrones from four different sets of stellar evolution models. Using these values for the stellar parameters we reanalyze the transit data and derive an orbital inclination of i = 85.2 +/- 1.4 degrees. For the planet we derive a mass of Mp = 0.69 +/- 0.05 Jupiter masses, a radius of Rp = 1.54 +/- 0.18 Jupiter radii, and a density of 0.23 +/- 0.08 grams per cubic cm.Comment: 11 pages, 1 figure, 2 tables, LaTex, aastex, accepted for publication by ApJ Letter

Quantitative analysis of stover quality in the Lancaster variety of corn

Peer Reviewe

Perspectives in the selection of a semiexotic maize for forage use

Peer Reviewe

A search for p-modes and other variability in the binary system 85 Pegasi using MOST photometry

Context: Asteroseismology has great potential for the study of metal-poor stars due to its sensitivity to determine stellar ages. Aims: Our goal was to detect p-mode oscillations in the metal-poor sub-dwarf 85 Peg A and to search for other variability on longer timescales. Methods: We have obtained continuous high-precision photometry of the binary system 85 Pegasi with the MOST space telescope in two seasons (2005 & 2007). Furthermore, we redetermined vsini for 85 Peg A using high resolution spectra obtained through the ESO archive, and used photometric spot modeling to interpret long periodic variations. Results: Our frequency analysis yields no convincing evidence for p-modes significantly above a noise level of 4 ppm. Using simulated p-mode patterns we provide upper RMS amplitude limits for 85 Peg A. The light curve shows evidence for variability with a period of about 11 d and this periodicity is also seen in the follow up run in 2007; however, as different methods to remove instrumental trends in the 2005 run yield vastly different results, the exact shape and periodicity of the 2005 variability remain uncertain. Our re-determined vsini value for 85 Peg A is comparable to previous studies and we provide realistic uncertainties for this parameter. Using these values in combination with simple photometric spot models we are able to reconstruct the observed variations. Conclusions: The null-detection of p-modes in 85 Peg A is consistent with theoretical values for pulsation amplitudes in this star. The detected long-periodic variation must await confirmation by further observations with similar or better precision and long-term stability. If the 11 d periodicity is real, rotational modulation of surface features on one of the components is the most likely explanation.Comment: 11 pages, 9 figures, accepted for publication in A&

HD 174884: a strongly eccentric, short-period early-type binary system discovered by CoRoT

Accurate photometric CoRoT space observations of a secondary seismological target, HD 174884, led to the discovery that this star is an astrophysically important double-lined eclipsing spectroscopic binary in an eccentric orbit (e of about 0.3), unusual for its short (3.65705d) orbital period. The high eccentricity, coupled with the orientation of the binary orbit in space, explains the very unusual observed light curve with strongly unequal primary and secondary eclipses having the depth ratio of 1-to-100 in the CoRoT 'seismo' passband. Without the high accuracy of the CoRoT photometry, the secondary eclipse, 1.5 mmag deep, would have gone unnoticed. A spectroscopic follow-up program provided 45 high dispersion spectra. The analysis of the CoRoT light curve was performed with an adapted version of PHOEBE that supports CoRoT passbands. The final solution was obtained by simultaneous fitting of the light and the radial velocity curves. Individual star spectra were derived by spectrum disentangling. The uncertainties of the fit were derived by bootstrap resampling and the solution uniqueness was tested by heuristic scanning. The results provide a consistent picture of the system composed of two late B stars. The Fourier analysis of the light curve fit residuals yields two components, with orbital frequency multiples and an amplitude of about 0.1 mmag, which are tentatively interpreted as tidally induced pulsations. An extensive comparison with theoretical models is carried out by means of the Levenberg-Marquardt minimization technique and the discrepancy between models and the derived parameters is discussed. The best fitting models yield a young system age of 125 million years which is consistent with the eccentric orbit and synchronous component rotation at periastron.Comment: 15 pages, 12 figures. Accepted for publication by A&

A Spitzer Transmission Spectrum for the Exoplanet GJ 436b, Evidence for Stellar Variability, and Constraints on Dayside Flux Variations

In this paper we describe a uniform analysis of eight transits and eleven secondary eclipses of the extrasolar planet GJ 436b obtained in the 3.6, 4.5, and 8.0 micron bands using the IRAC instrument on the Spitzer Space Telescope between UT 2007 June 29 and UT 2009 Feb 4. We find that the best-fit transit depths for visits in the same bandpass can vary by as much as 8% of the total (4.7 sigma significance) from one epoch to the next. Although we cannot entirely rule out residual detector effects or a time-varying, high-altitude cloud layer in the planet's atmosphere as the cause of these variations, we consider the occultation of active regions on the star in a subset of the transit observations to be the most likely explanation. We reconcile the presence of magnetically active regions with the lack of significant visible or infrared flux variations from the star by proposing that the star's spin axis is tilted with respect to our line of sight, and that the planet's orbit is therefore likely to be misaligned. These observations serve to illustrate the challenges associated with transmission spectroscopy of planets orbiting late-type stars; we expect that other systems, such as GJ 1214, may display comparably variable transit depths. Our measured 8 micron secondary eclipse depths are consistent with a constant value, and we place a 1 sigma upper limit of 17% on changes in the planet's dayside flux in this band. Averaging over the eleven visits gives us an improved estimate of 0.0452% +/- 0.0027% for the secondary eclipse depth. We combine timing information from our observations with previously published data to produce a refined orbital ephemeris, and determine that the best-fit transit and eclipse times are consistent with a constant orbital period. [ABRIDGED]Comment: 26 pages, 18 figures, 7 tables in emulateapj format. Accepted for publication in Ap

Cellular Ser/Thr-Kinase Assays Using Generic Peptide Substrates

High-throughput cellular profiling has successfully stimulated early drug discovery pipelines by facilitating targeted as well as opportunistic lead finding, hit annotation and SAR analysis. While automation-friendly universal assay formats exist to address most established drug target classes like GPCRs, NHRs, ion channels or Tyr-kinases, no such cellular assay technology is currently enabling an equally broad and rapid interrogation of the Ser/Thr-kinase space. Here we present the foundation of an emerging cellular Ser/Thr-kinase platform that involves a) coexpression of targeted kinases with promiscuous peptide substrates and b) quantification of intracellular substrate phosphorylation by homogeneous TR-FRET. Proof-of-concept data is provided for cellular AKT, B-RAF and CamK2δ assays. Importantly, comparable activity profiles were found for well characterized B-Raf inhibitors in TR-FRET assays relying on either promiscuous peptide substrates or a MEK1(WT) protein substrate respectively. Moreover, IC50-values correlated strongly between cellular TR-FRET assays and a gold standard Ba/F3 proliferation assay for B-Raf activity. Finally, we expanded our initial assay panel by screening a kinase-focused cDNA library and identified starting points for >20 cellular Ser/Thr-kinase assays