Selecting methods for ecosystem service assessment: a decision tree approach

A range of methods are available for assessing ecosystem services. Methods differ in their aims; from mapping and modelling the supply and demand of ecosystem services to appraising their economic and non-economic importance through valuation techniques. Comprehensive guidance for the selection of appropriate ecosystem service assessment methods that address the requirements of different decision-making contexts is lacking. This paper tackles this gap using the experience from 27 case studies which applied different biophysical, socio-cultural and monetary valuation methods to operationalise the ecosystem service concept towards sustainable land, water and urban management. A survey of the reasons why the case study teams selected particular methods revealed that stakeholder-oriented reasons, such as stakeholder participation, inclusion of local knowledge and ease of communication, and decision-oriented reasons, such as the purpose of the case study and the ecosystem services at stake, were key considerations in selecting a method. Pragmatic reasons such as available data, resources and expertise were also important factors. This information was used to develop a set of linked decision trees, which aim to provide guidance to researchers and practitioners in choosing ecosystem service assessment methods that are suitable for their context

Caspofungin Dose Escalation for Invasive Candidiasis Due to Resistant Candida albicans ▿

Previous in vivo studies have reported caspofungin dose escalation to be effective against Candida glabrata with reduced susceptibility. We hypothesized that higher doses of caspofungin would be effective against invasive candidiasis caused by the more virulent species Candida albicans, including isolates resistant to this echinocandin. Immunocompetent mice were inoculated with one of three C. albicans isolates, including one susceptible and two resistant isolates with different FKS1 hot spot 1 point mutations. Mice received daily caspofungin treatment for 7 days and were then followed off therapy for 2 weeks to assess survival. Kidney tissue and blood were collected, and fungal burden and serum (1→3)-β-d-glucan were measured. Significant differences in virulence were observed among the three C. albicans isolates, which translated into differences in responses to caspofungin. The most virulent of the resistant isolates studied (isolate 43001; Fks1p F641S) did not respond to caspofungin doses of up to 10 mg/kg of body weight, as there were no differences in survival (survival range, 0 to 12% with treatment), tissue burden, or (1→3)-β-d-glucan concentration compared to those for untreated controls. Higher doses of caspofungin did improve survival against the second resistant isolate (53264; Fks1p S645P) that demonstrated reduced virulence (5 and 10 mg/kg; 80% survival). In contrast, caspofungin doses as low as 1 mg/kg improved survival (85 to 95%) and reduced tissue burden and (1→3)-β-d-glucan concentration against the susceptible isolate (ATCC 90028). These data suggest that caspofungin dose escalation for invasive candidiasis may not be consistently effective against resistant C. albicans isolates, and this may be associated with the virulence of the strain