Euclid space mission: a cosmological challenge for the next 15 years

Euclid is the next ESA mission devoted to cosmology. It aims at observing most of the extragalactic sky, studying both gravitational lensing and clustering over $\sim$15,000 square degrees. The mission is expected to be launched in year 2020 and to last six years. The sheer amount of data of different kinds, the variety of (un)known systematic effects and the complexity of measures require efforts both in sophisticated simulations and techniques of data analysis. We review the mission main characteristics, some aspects of the the survey and highlight some of the areas of interest to this meetingComment: to appear in Proceedings IAU Symposium No. 306, 2014, "Statistical Challenges in 21st Century Cosmology", A.F. Heavens, J.-L. Starck & A. Krone-Martins, ed

Restoration of catalytic functions in Cre recombinase mutants by electrostatic compensation between active site and DNA substrate

Two conserved catalytic arginines, Arg-173 and Arg-292, of the tyrosine site-specific recombinase Cre are essential for the transesterification steps of strand cleavage and joining in native DNA substrates containing scissile phosphate groups. The active site tyrosine (Tyr-324) provides the nucleophile for the cleavage reaction, and forms a covalent 3′-phosphotyrosyl intermediate. The 5′-hydroxyl group formed during cleavage provides the nucleophile for the joining reaction between DNA partners, yielding strand exchange. Previous work showed that substitution of the scissile phosphate (P) by methylphosphonate (MeP) permits strand cleavage by a Cre variant lacking Arg-292. We now demonstrate that MeP activation and cleavage are not blocked by substitution of Arg-173 or even simultaneous substitutions of Arg-173 and Arg-292 by alanine. Furthermore, Cre(R173A) and Cre(R292A) are competent in strand joining, Cre(R173A) being less efficient. No joining activity is detected with Cre(R173A, R292A). Consistent with their ability to cleave and join strands, Cre(R173A) and Cre(R292A) can promote recombination between two MeP-full-site DNA partners. These findings shed light on the overall contribution of active site electrostatics, and tease apart distinctive contributions of the individual arginines, to the chemical steps of recombination. They have general implications in active site mechanisms that promote important phosphoryl transfer reactions in nucleic acids

Cellular shear adhesion force measurement and simultaneous imaging by atomic force microscope

This paper presents a sensitive and fast cellular shear adhesion force measurement method using an atomic force microscope (AFM). In the work, the AFM was used both as a tool for the imaging of cells on the nano-scale and as a force sensor for the measurement of the shear adhesion force between the cell and the substrate. After the cell imaging, the measurement of cellular shear adhesion forces was made based on the different positions of the cell on the nano-scale. Moreover, different pushing speeds of probe and various locations of cells were used in experiments to study their influences. In this study, the measurement of the cell adhesion in the upper portion of the cell is different from that in the lower portion. It may reveal that the cancer cells have the metastasis tendency after cultured for 16 to 20 hours, which is significant for preventing metastasis in the patients diagnosed with early cancer lesions. Furthermore, the cellular shear adhesion forces of two types of living cancer cells were obtained based on the measurements of AFM cantilever deflections in the torsional and vertical directions. The results demonstrate that the shear adhesion force of cancer cells is twice as much as the same type of cancer cells with TRAIL. The method can also provide a way for the measurement of the cellular shear adhesion force between the cell and the substrate, and for the simultaneous exploration of cells using the AFM imaging and manipulatio

Determination of work of adhesion of biological cell under AFM bead indentation

Hertz contact theory has been widely used for the determination of cell elasticity based on AFM indentation experiments. In light of the adhesive contact between AFM tip and cell, this study applied Johnson–Kendall–Roberts (JKR) model to fit the indentation force–displacement (F–D) curves reported previously. A MIN6 cell has been modeled as first a sphere and then a flattened cell with different thicknesses. The results have shown that both basic JKR model and “generalized” JKR model can best describe the unloading force–displacement behaviors of the indentation curves. The Young׳s modulus of the cell and the work of adhesion of the cell–indenter interface are obtained. In comparison to the Hertzian contact model, the JKR model provides obviously better fitting to the experimental results, indicating that the adhesion is significant in the cell interaction

A conceptual modeling methodology based on niches and granularity

This paper presents a methodology for conceptual modeling which is based on a new modeling primitive, the niche, and associated constructs granularity and reconciliation. A niche is an environment where entities interact for a specific purpose, playing specific roles, and according to the norms and constraints of that environment. Granularity refers to the relative level of power or influence of an entity within a niche. Reconciliation is a relationship from N entities onto one reconciled entity, and represents explicitly a situation where two or more different perspectives of the same entity have been reconciled, by negotiation, into a single consensus view. The methodology we propose provides a systematic method of designing conceptual models along with a process for normalising inappropriate relationships. Normalising is a prescriptive process for identifying and remedying inconsistencies within a model based on granularities. Drawing on a number of case studies, we show how niches and granularity make complexity easier to manage, highlight inaccuracies in a model, identify opportunities for achieving project goals, and reduce semantic heterogeneity

Euclid preparation XVIII. The NISP photometric system

Euclid will be the first space mission to survey most of the extragalactic sky in the 0.95–2.02 µm range, to a 5 σ point-source median depth of 24.4 AB mag. This unique photometric dataset will find wide use beyond Euclid’s core science. In this paper, we present accurate computations of the Euclid YE, JE, and HE passbands used by the Near-Infrared Spectrometer and Photometer (NISP), and the associated photometric system. We pay particular attention to passband variations in the field of view, accounting for, among other factors, spatially variable filter transmission and variations in the angle of incidence on the filter substrate using optical ray tracing. The response curves’ cut-on and cut-off wavelengths – and their variation in the field of view – are determined with ∼0.8 nm accuracy, essential for the photometric redshift accuracy required by Euclid. After computing the photometric zero points in the AB mag system, we present linear transformations from and to common ground-based near-infrared photometric systems, for normal stars, red and brown dwarfs, and galaxies separately. A Python tool to compute accurate magnitudes for arbitrary passbands and spectral energy distributions is provided. We discuss various factors, from space weathering to material outgassing, that may slowly alter Euclid’s spectral response. At the absolute flux scale, the Euclid in-flight calibration program connects the NISP photometric system to Hubble Space Telescope spectrophotometric white dwarf standards; at the relative flux scale, the chromatic evolution of the response is tracked at the milli-mag level. In this way, we establish an accurate photometric system that is fully controlled throughout Euclid’s lifetime

Euclid preparation. XVIII. The NISP photometric system

Euclid will be the first space mission to survey most of the extragalactic sky in the 0.95-2.02 $\mu$m range, to a 5$\sigma$ point-source median depth of 24.4 AB mag. This unique photometric data set will find wide use beyond Euclid's core science. In this paper, we present accurate computations of the Euclid Y_E, J_E and H_E passbands used by the Near-Infrared Spectrometer and Photometer (NISP), and the associated photometric system. We pay particular attention to passband variations in the field of view, accounting among others for spatially variable filter transmission, and variations of the angle of incidence on the filter substrate using optical ray tracing. The response curves' cut-on and cut-off wavelengths - and their variation in the field of view - are determined with 0.8 nm accuracy, essential for the photometric redshift accuracy required by Euclid. After computing the photometric zeropoints in the AB mag system, we present linear transformations from and to common ground-based near-infrared photometric systems, for normal stars, red and brown dwarfs, and galaxies separately. A Python tool to compute accurate magnitudes for arbitrary passbands and spectral energy distributions is provided. We discuss various factors from space weathering to material outgassing that may slowly alter Euclid's spectral response. At the absolute flux scale, the Euclid in-flight calibration program connects the NISP photometric system to Hubble Space Telescope spectrophotometric white dwarf standards; at the relative flux scale, the chromatic evolution of the response is tracked at the milli-mag level. In this way, we establish an accurate photometric system that is fully controlled throughout Euclid's lifetime.Comment: 33 pages, 25 figures, accepted for publication in A&

Planck early results I : The Planck mission

Peer reviewe