Abiotrofia cerebelar em um gato: relato de caso

RESUMO Um caso de abiotrofia cerebelar em um gato com 45 dias de idade foi diagnosticado no Laboratório de Patologia Animal, Hospital Veterinário da Universidade Federal de Campina Grande. O animal apresentava, havia 15 dias, apatia, anorexia, desidratação, ataxia, hipermetria, espasticidade dos membros torácicos e pélvicos, tremores de intenção, nistagmo, opistótono, déficit proprioceptivo e ausência de resposta de ameaça. Clinicamente, havia a suspeita de hipoplasia cerebelar, e, devido ao prognóstico desfavorável, o animal foi eutanasiado. Na necropsia, não foram observadas alterações macroscópicas. Microscopicamente, as lesões estavam restritas ao cerebelo e caracterizavam-se por alterações neurodegenerativas e necróticas, com desaparecimento segmentar dos neurônios de Purkinje. Nessas áreas, também se verificaram espaços em branco, denominado aspecto de cesto vazio, resultantes da perda dos neurônios de Purkinje, além de raros esferoides axonais e proliferação dos astrócitos de Bergmann. Em algumas áreas, a camada granular estava hipocelular e havia moderada gliose multifocal na camada molecular. O diagnóstico de abiotrofia cerebelar foi realizado com base nos dados epidemiológicos, clínicos e, principalmente, pelas alterações histopatológicas dos neurônios de Purkinje características da doença

Intestinal intussusception secondary to enteritis caused by Pythium insidiosum in a bitch: case report

ABSTRACT We report a case of intestinal intussusception secondary to enteritis caused by Pythium insidiosum infection in a 1-year-old mixed breed bitch that died after a 30-day history of bloody diarrhea, anorexia, weight loss, listlessness, dehydration, and pale mucous membranes. Necropsy revealed two areas of intussusception, one jejunum-jejunal and one ileum-cecum-colic. The first intussusception showed slight congestion of the intestinal loop, which was resolved by manual traction, while the second intussusception exhibited thickening, irregular yellow spots, and transmural congestion. Histologically, the jejunum-jejunal segment had ischemic lesions secondary and ileum-cecum-colic intussusception there was also necrotizing, pyogranulomatous enteritis associated with negative images of intralesional fungal hyphae that were well visualized by silver impregnation. Immunohistochemistry identified the hyphae as Pythium insidiosum. The diagnosis was made based on the histological changes and confirmed by immunohistochemistry. We can conclude that enteritis by P. insidiosum in dogs can occasionally cause intestinal intussusception and result in acute death

Brewer’s Spent Dry Yeast Modulates Immunity in Gilthead Sea Bream (Sparus aurata)

In this study, we evaluated the replacement of dietary protein sources like fishmeal (FM) and plant proteins (PP) by Brewer’s spent dry yeast (SDY) on the transcriptomic response (RNA-seq, NextSeq500 platform Illumina) in the liver, anterior-mid intestine, and head kidney in juveniles of gilthead sea bream (Sparus aurata). The inclusion of SDY at 30% in the experimental diet (40% crude protein, 16% crude lipid) resulted in a reduction in FM (10%) and PP (31.4%) contents. Using RNA-seq, a total of 19.4 million raw reads per library were obtained, from whose 99.8% of the sequenced data were retained. The alignment efficiency of uniquely mapped reads was 90.3, 89.5, and 89.8% for head kidney, liver, and anterior-mid intestine, respectively. In total, 218 differentially expressed genes (DEGs) were identified among all tissues, out of which, 141 were up- and 77 down-regluated. The enrichment analysis of DEGs revealed that SDY had a modulatory effect on several processes related to host’s immunity, oxygen’s carrier capacity, steroidogenesis, metabolism, and digestion. It is of special relevance the immunomodulatory effects of the tested ingredient as data from RNA-seq showed from the three target tissues analyzed. These results indicated that this ingredient in addition to being considered as a sustainable raw material for replacing conventional protein sources in aquafeeds may also be considered as a functional ingredient due to its content in β-glucans. The overall results of this study coupled with previous nutritional studies on this ingredient indicated the suitability of brewery’s by-products like SDY in aquafeeds for carnivorous species like gilthead seabream, as well as supporting a circular bioeconomy model that reuses, recovers, and recycles resources instead of producing wastes.info:eu-repo/semantics/publishedVersio

The BaBar detector: Upgrades, operation and performance

Contains fulltext : 121729.pdf (preprint version ) (Open Access