A new method for extracting conodonts and radiolarians from chert with NaOH solution

Microfossils are important components of sedi- mentary rocks used for palaeontological, biostratigraphic, palaeoenvironmental and palaeoclimatic investigations. They are usually extracted from rocks using an acid solution, which might vary depending on the embedding rock lithology. Here we propose a new method using common NaOH (sodium hydroxide; soda) to digest cherts (micro- and cryptocrystalline quartz) instead of the standard technique based on HF (hydrofluoric acid). This new method allows the collection of undamaged specimens of different kinds of microfossils, such as conodonts, radiolarians, teeth and dermal scales, the miner- ology of which is still preserved (e.g. biogenic apatite in cono- donts). The use of soda is thus recommended, as it is less dangerous, less expensive, and it better preserves the extracted microfossils both in shape and mineralogy

The Special LHC Interconnections: Technologies, Organization and Quality Control

In addition to the standard interconnections (IC) of the continuous cryostat of the Large Hadron Collider (LHC), there exists a variety of special ones related to specific components and assemblies, such as cryomagnets of the insertion regions, electrical feedboxes and superconducting links. Though they are less numerous, their specificities created many additional IC types, requiring a larger variety of assembly operations and quality control techniques, keeping very high standards of quality. Considerable flexibility and adaptability from all the teams involved (CERN staff, collaborating institutes, contractors) were the key points to ensure the success of this task. This paper first describes the special IC and presents the employed technologies which are generally adapted from the standard work. Then, the organization adopted for this non-repetitive work is described. Examples of non-conformities that were resolved are also discussed. Figures of merit in terms of quality and productivity are given and compared with standard IC wor

The Quality Control of the LHC Continuous Cryostat Interconnections

The interconnections between the Large Hadron Collider (LHC) magnets have required some 40 000 TIG welded joints and 65 000 electrical splices. At the level of single joints and splices, non-destructive techniques find limited application: quality control is based on the qualification of the process and of operators, on the recording of production parameters and on production samples. Visual inspection and process audits were the main techniques used. At the level of an extended chain of joints and splices - from a 53.5 m half-cell to a complete 2.7 km arc sector - quality control is based on vacuum leak tests, electrical tests and RF microwave reflectometry that progressively validated the work performed. Subsequent pressure tests, cryogenic circuits flushing with high pressure helium and cool-downs revealed a few unseen or new defects. This paper presents an overview of the quality control techniques used, seeking lessons applicable to similar large, complex projects

The LHC Continuous Cryostat Interconnections: The Organization of a Logistically Complex Worksite Requiring Strict Quality Standards and High Output

The interconnections of the Large Hadron Collider (LHC) continuous cryostat have been completed in fall 2007: 1695 interconnections magnet to magnet and 224 interconnections between the continuous cryostat and the cryogenic distribution line have been executed along the 27 km of the LHC. The very tight schedule, the complexity of the interconnection sequence, the strict quality standards applied have required the creation of an ad hoc organization in order to steer and coordinate the activities on the worksite dispersed along the whole accelerator ring. The concatenation of construction and test phases carried out by CERN staff, CERN collaborating institutes and contractors have led to the necessity of a common approach and of a very effective information flow. In this paper, after having recalled the main technical challenges, we review the organizational choices that have been taken and we briefly analyze the development of the worksite in term of allocated resources and production

High resolution structures of mutants of residues that affect access to the ligand-binding cavity of human lipocalin-type prostaglandin D Synthase

Lipocalin-type prostaglandin D synthase (L-PGDS) catalyzes the isomerisation of the 9,11-endoperoxide group of PGH2 (Prostaglandin H2) to produce PGD2 (Prostaglandin D2) with 9-hydroxy and 11-keto groups. The product of the reaction, PGD2, is the precursor of several metabolites involved in many regulatory events. L-PGDS, the first member of the important lipocalin family to be recognized as an enzyme, is also able to bind and transport small hydrophobic molecules and was formerly known as \u3b2-trace protein, the second most abundant protein in human cerebro-spinal fluid. Previous structural work on the mouse and human proteins has focused on the identification of the amino acids responsible and the proposal of a mechanism for catalysis. In this paper we present the X-ray structures of the apo and holo forms (bound to PEG) of the C65A mutant of human L-PGDS to 1.40 \uc5 resolution and of the double mutant C65A K59A to 1.60 \uc5 resolution. We have also studied the apo forms of the double mutants C65A W54F and C65A W112F and the triple mutant C65A W54F W112F. Mutation of the lysine residue does not seem to affect the binding of PEG to the ligand-binding cavity and mutation of a single or both tryptophanes appears to have the same effect on the position of these two aromatic residues at the entrance of the cavity. We have also identified a solvent molecule in an invariant position in the cavity of virtually all the molecules present in the 9 asymmetric units of the crystals that we have examined. Taken together our observations indicate that the residues we have mutated appear to indeed play a role in the entrance-exit process of the substrate and/or other ligands to the binding cavity of the lipocalin

High resolution structures of mutants of residues that affect access to the ligand-binding cavity of human lipocalin-type prostaglandin D synthase

Lipocalin-type prostaglandin D synthase (L-PGDS) catalyzes the isomerisation of the 9,11-endoperoxide group of PGH2 (Prostaglandin H2) to produce PGD2 (Prostaglandin D2) with 9-hydroxy and 11-keto groups. The product of the reaction, PGD2, is the precursor of several metabolites involved in many regulatory events. L-PGDS, the first member of the important lipocalin family to be recognized as an enzyme, is also able to bind and transport small hydrophobic molecules and was formerly known as \u3b2-trace protein, the second most abundant protein in human cerebro-spinal fluid. Previous structural work on the mouse and human proteins has focused on the identification of the amino acids responsible and the proposal of a mechanism for catalysis. In this paper we present the X-ray structures of the apo and holo forms (bound to PEG) of the C65A mutant of human L-PGDS to 1.40 \uc5 resolution and of the double mutant C65A K59A to 1.60 \uc5 resolution. We have also studied the apo forms of the double mutants C65A W54F and C65A W112F and the triple mutant C65A W54F W112F. Mutation of the lysine residue does not seem to affect the binding of PEG to the ligand-binding cavity and mutation of a single or both tryptophanes appears to have the same effect on the position of these two aromatic residues at the entrance of the cavity. We have also identified a solvent molecule in an invariant position in the cavity of virtually all the molecules present in the 9 asymmetric units of the crystals that we have examined.Taken together our observations indicate that the residues we have mutated appear to indeed play a role in the entrance-exit process of the substrate and/or other ligands to the binding cavity of the lipocalin

Mechanical Design Aspects of The LHC Beam screen

Forty-four kilometers of the LHC beam vacuum system [1,2] will be equipped with a perforated co-axial liner, the so-called beam screen. Operating between 5 K and 20 K, the beam screen reduces heat loads to the 1.9 K helium bath of the superconducting magnets and minimises dynamic vacuum effects. Constructed from low magnetic permeability stainless steel with a 50 mm inner layer of high purity copper, the beam screen must provide a maximum aperture for the beam whilst resisting the induced forces due to eddy currents at magnet quench. The mechanical engineering challenges are numerous, and include stringent requirements on geometry, material selection, manufacturing techniques and cleanliness. The industrial fabrication of these 16 metre long UHV components is now in its prototyping phase. A description of the beam screen is given, together with details of the experimental programme aimed at validating the design choices, and results of the first industrial prototypes

Bio-chronostratigraphic calibration of the Upper Carnian-Lower Norian magnetostratigraphic scale at Pizzo Mondello (Sicani Mountains, Sicily).

Pizzo Mondello section is known since 15 years because of the continuous Late Triassic pelagic record of great significance for the establishment of an integrated chronostratigraphy of the Late Triassic (Gullo et al. 1996; Muttoni et al. 2001, 2004). During the last 4 years, Pizzo Mondello section has been studied in detail to provide a new and high resolution integrated bio-chronostratigraphy for the calibration of the magnetostratigraphy and chemostratigraphy proposed by Muttoni et al. (2001, 2004), and now it is one of the GSSP candidates for the definition of the base of the Norian. The lowest 143 m of the Cherty Limestone, straddling the C/N boundary have been studied in detail. The preliminary data of the ongoing research have been presented in all the meetings of the STS from Albuquerque 2007 and here we summarize the final results. The key correlation to the standard marine Triassic Scale is provided by the ammonoids. They are relatively rare, however the available collections document the Upper Carnian Discotropites plinii and Gonionotites italicus Subzones, from meter 15 to meter 80 from the base of the section. The following 15 meters are poor in ammonoids, while higher up the lower part of the Lower Norian Guembelites jandianus Zone is documented by Dimorphites cf. n. sp.1 of Krystyn, 1980. Conodonts are very abundant and have a great potential as practical tool for global correlations. The abundance of specimens at Pizzo Mondello gave the opportunity to point out clear relationships among the five most widespread Upper Camian/Lower Norian conodont genera (Paragondolella, Carnepigondolella, Metapolygnathus, Epigondolella and Norigondolella) and to identify trends of the generic turnovers (Mazza et al. 2010). The two biomarkers so far proposed as possible marker events for the GSSP were the FAD of E. quadrata (sample FNP88A) and the FAD of M. communisti (sample NA35). However, the FAD of E. quadrata occurs within the Gonionotites italicus Subzone, while the FAD of M. communisti is on its top. Halobiids are extremely common in the Cherty Limestone and they have also a great potential for large scale correlations. Nine species of Halobia have been recognized: Halobia carnica, H. lenticularis, H. simplex, H. superba, H. cf. rugosa, H. radiata, H. austriaca, H. styriaca and H. mediterranea. The best possible marker for the base of the Norian is the first occurrence of Halobia austriaca, that is recorded in the middle of the interval between the record of the Gonionotites italicus Subzone and the Guembelites jandianus Zone. Radiolarians were found in few samples but with high diversity assemblages. In the upper Gonionotites italicus Subzone to the Guembelites jandianus Zone there is an overlap of species previously considered Late Carnian with species usually regarded as Early Norian. About 4 m above the FAD of E. quadrata, in the Gonionotites italicus Subzone, the first assemblage with Capnuchosphaera deweveri Kozur & Mostler, Capnuchosphaera tricornis De Wever, Kahlerosphaera norica Kozur & Mock and Xiphothecaella longa Kozur & Mock, usually referred to Early Norian, occurs. These integrated bio-chronostratigraphic studies lead to identify some possible GSSP marker events especially on conodonts and halobiids, which occur in the upper part of magnetozone PM 4n, within PM 4r and in the lower part of PM 5n. Possibly the most suitable magnetostratigraphic event to recognize the basal Norian is the base of magnetozone PM 5n, as already suggested by Krystyn et al. 2002 and Muttoni et al. 2004

Single hadron response measurement and calorimeter jet energy scale uncertainty with the ATLAS detector at the LHC

The uncertainty on the calorimeter energy response to jets of particles is derived for the ATLAS experiment at the Large Hadron Collider (LHC). First, the calorimeter response to single isolated charged hadrons is measured and compared to the Monte Carlo simulation using proton-proton collisions at centre-of-mass energies of sqrt(s) = 900 GeV and 7 TeV collected during 2009 and 2010. Then, using the decay of K_s and Lambda particles, the calorimeter response to specific types of particles (positively and negatively charged pions, protons, and anti-protons) is measured and compared to the Monte Carlo predictions. Finally, the jet energy scale uncertainty is determined by propagating the response uncertainty for single charged and neutral particles to jets. The response uncertainty is 2-5% for central isolated hadrons and 1-3% for the final calorimeter jet energy scale.Comment: 24 pages plus author list (36 pages total), 23 figures, 1 table, submitted to European Physical Journal

Measurements of Higgs boson production and couplings in diboson final states with the ATLAS detector at the LHC

Measurements are presented of production properties and couplings of the recently discovered Higgs boson using the decays into boson pairs, H →γ γ, H → Z Z∗ →4l and H →W W∗ →lνlν. The results are based on the complete pp collision data sample recorded by the ATLAS experiment at the CERN Large Hadron Collider at centre-of-mass energies of √s = 7 TeV and √s = 8 TeV, corresponding to an integrated luminosity of about 25 fb−1. Evidence for Higgs boson production through vector-boson fusion is reported. Results of combined fits probing Higgs boson couplings to fermions and bosons, as well as anomalous contributions to loop-induced production and decay modes, are presented. All measurements are consistent with expectations for the Standard Model Higgs boson